| Budget Amount *help |
¥19,890,000 (Direct Cost: ¥15,300,000、Indirect Cost: ¥4,590,000)
Fiscal Year 2012: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2011: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2010: ¥10,530,000 (Direct Cost: ¥8,100,000、Indirect Cost: ¥2,430,000)
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| Research Abstract |
I show that a newly-developed second-order nonlinear spectroscopic technique allows me to draw topology diagrams of such proteins. Uniquely, one can determine "up" versus "down" alignment of molecules on a surface using this second-order nonlinear spectroscopy. In my novel but simple approach, an "alignment indicator" is introduced to each segment of a protein before immobilizing the protein on a glass surface, and the up versus down alignment of each segment is nonlinear-spectroscopically determined. This approach is very powerful and useful for proteins to which neither X-ray nor NMR is applicable.
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