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Development of microspectroscopic method measuring faint absorbance of transition metal complexes existing at liquid/liquid interface and evaluation of their interfacial functions

Research Project

Project/Area Number 22350034
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Analytical chemistry
Research InstitutionOsaka University (2011-2012)
Hiroshima University (2010)

Principal Investigator

TSUKAHARA Satoshi  大阪大学, 大学院・理学研究科, 教授 (50207338)

Co-Investigator(Kenkyū-buntansha) FUJIWARA Terufumi  広島大学, 大学院・理学研究科, 教授 (80127703)
OKAMOTO Yasuaki  広島大学, 大学院・理学研究科, 助教 (40213988)
Project Period (FY) 2010 – 2012
Project Status Completed (Fiscal Year 2012)
Budget Amount *help
¥13,780,000 (Direct Cost: ¥10,600,000、Indirect Cost: ¥3,180,000)
Fiscal Year 2012: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Fiscal Year 2011: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2010: ¥6,630,000 (Direct Cost: ¥5,100,000、Indirect Cost: ¥1,530,000)
Keywords液液界面 / 遷移金属錯体 / in situ顕微分光計測 / 極微吸収量 / 配向性 / 極微吸光量 / 界面機能
Research Abstract

In the present study, we mainly developed the following two methods, 1. measurements of brightness of small regions of summarized microscope images with a high sensitivity, 2. measurements of brightness of small regions using polarized light with a ultrahigh sensitivity. These methods enabled us to measure faint absorbances and absorption spectra of monolayers of tetraphenylporphinatomanganese(III) formed at the dodecane-toluene/water interface and those of chlorophyll a and b in Paramecium bursaria and Euglena, which belong to protozoa.

Report

(4 results)
  • 2012 Annual Research Report   Final Research Report ( PDF )
  • 2011 Annual Research Report
  • 2010 Annual Research Report
  • Research Products

    (43 results)

All 2013 2012 2011 2010 Other

All Journal Article (14 results) (of which Peer Reviewed: 14 results) Presentation (26 results) (of which Invited: 4 results) Remarks (3 results)

  • [Journal Article] TSUKAHARA, Satoshi, "Microscope Measurements of Lateral Diffusion of Fluorescent Rhodamine B at Toluene/Water Interface by Total Internal Reflection-Fluorescence Recovery after Photobleaching2013

    • Author(s)
      SHINOMORI, Naoki
    • Journal Title

      Chem. Lett

      Volume: 42 Issue: 4 Pages: 444-446

    • DOI

      10.1246/cl.121223

    • NAID

      10031162202

    • Related Report
      2012 Annual Research Report 2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Surface tension determination through measurements of resonance oscillation of a small surface using dielectric force by a localized alternating current electric field2013

    • Author(s)
      TSUKAHARA, Satoshi; TSURUTA, Tsuyoshi; FUJIWARA, Terufumi
    • Journal Title

      Analyst

      Volume: 138 Issue: 7 Pages: 2110-2117

    • DOI

      10.1039/c3an36260d

    • Related Report
      2012 Annual Research Report 2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Single-Molecule Lactonization of Octadecylrhodamine B at a Liquid-Liquid Interface2012

    • Author(s)
      MATSUI, Tomoko; TSUKAHARA, Satoshi; WATARAI, Hitoshi
    • Journal Title

      Langmuir

      Volume: 28 Issue: 44 Pages: 15428-15432

    • DOI

      10.1021/la302375z

    • Related Report
      2012 Annual Research Report 2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Chirality induction and amplification in methylene blue H-aggregates via D- and L-phenylalanine pre-adsorbed on the tungsten oxide nanocolloid surface2012

    • Author(s)
      ADACHI, Kenta; TANAKA, Shohei; YAMAZAKI, Suzuko; TAKECHI, Hideaki; TSUKAHARA, Satoshi; WATARAI, Hitoshi
    • Journal Title

      New J. Chem

      Volume: 36 Issue: 11 Pages: 2167-2170

    • DOI

      10.1039/c2nj40415j

    • NAID

      120005349036

    • Related Report
      2012 Annual Research Report 2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Charge Simulation Method Calculation of the Inhomogeneous Electric Field near the Dodecane/Water Interface Generated by Two Needle Electrodes2012

    • Author(s)
      TSUKAHARA, Satoshi; MATSUMOTO, Yuji; URASAKI, Singo; MURAMATSU, Masanori; FUJIWARA, Terufumi
    • Journal Title

      Solvent Extr. Res. Dev., Jpn

      Volume: 19 Pages: 29-39

    • NAID

      130005056947

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Charge Simulation Method Calculation of the Inhomogeneous Electric Field near the Dodecane/Water Interface Generated by Two Needle Electrodes2012

    • Author(s)
      TSUKAHARA, Satoshi
    • Journal Title

      Solvent Extraction Research and Development, Japan

      Volume: 19 Pages: 29-39

    • NAID

      130005056947

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Microscope Measurements for the Transient Formation of W/O Emulsions of Sodium Bis(2-ethylhexyl) Sulfosuccinate in the Dodecane/Water Interfacial Region2011

    • Author(s)
      TSUKAHARA, Satoshi; SHISHINO, Yuichi; FUJIWARA, Terufumi
    • Journal Title

      Langmuir

      Volume: 27 Issue: 12 Pages: 7392-7399

    • DOI

      10.1021/la1045173

    • Related Report
      2012 Final Research Report 2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] In Situ Fluorescence Microscopic Measurements of the Phase Transition Behavior of Dipalmitoyl Phosphatidylcholine Monolayers Prepared at Hydrocarbon/Water Interfaces2011

    • Author(s)
      TSUKAHARA, Satoshi; MUKAI, Kazuaki; WATANABE, Shota; FUJIWARA, Terufumi
    • Journal Title

      Solvent Extr. Res. Dev., Jpn.

      Volume: 18 Pages: 149-158

    • NAID

      130005056923

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Chemiluminescence from an Oxidation Reaction of Rhodamine B with Cerium(IV) in a Reversed Micellar Medium of Cetyltrimethylammonium Chloride in 1-Hexanol-Cyclohexane/Water2011

    • Author(s)
      Tamer H. A. HASANIN, Yusuke TSUNEMINE, Satoshi TSUKAHARA, Yasuaki OKAMOTO, Terufumi FUJIWARA
    • Journal Title

      Analytical Sciences

      Volume: 27 Issue: 3 Pages: 297

    • DOI

      10.2116/analsci.27.297

    • NAID

      10027906323

    • ISSN
      0910-6340, 1348-2246
    • Related Report
      2012 Final Research Report 2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] In Situ Fluorescence Microscopic Measurements of the Phase Transition Behavior of Dipalmitoyl Phosphatidylcholine Monolayers Prepared at Hydrocarbon/Water Interfaces2011

    • Author(s)
      TSUKAHARA, Satoshi
    • Journal Title

      Solvent Extraction Research and Development, Japan

      Volume: 18 Pages: 149-158

    • NAID

      130005056923

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Chemiluminescence from an Oxidation Reaction of Rhodamine B with Cerium(IV) in a Reversed Micellar Medium of Cetyltrimethylammonium Chloride in 1-Hexanol-Cyclohexane/Water.2011

    • Author(s)
      Tamer H.A.HASANIN
    • Journal Title

      Anal.Sci.

      Volume: 27 Pages: 297-304

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] In Situ Fluorescence Microscopic Measurements of Phase Transition Behavior of Dipalmitoyl Phosphatidylcholine Monolayers Prepared at Hydrocarbon/Water Interfaces.2011

    • Author(s)
      Satoshi Tsukahara
    • Journal Title

      Solvent Extr.Res.Dev.Jpn.

      Volume: 18(掲載決定)

    • NAID

      130005056923

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Acid-Base Behavior of Rhodamine B in a Reversed Micellar Medium of Cetyltrimethylammonium Chloride in 1-Hexanol-Cyclohexane/Water2010

    • Author(s)
      Tamer H. A. HASANIN, Satoshi TSUKAHARA, Terufumi FUJIWARA
    • Journal Title

      Analytical Sciences

      Volume: 26 Issue: 12 Pages: 1247-1254

    • DOI

      10.2116/analsci.26.1247

    • NAID

      10027574764

    • ISSN
      0910-6340, 1348-2246
    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Acid-Base Behavior of Rhodamine B in a Reversed Micellar Medium of Cetyltrimethylammonium Chloride in 1-Hexanol-Cyclohexane/Water.2010

    • Author(s)
      Tamer H.A.HASANIN
    • Journal Title

      Anal.Sci.

      Volume: 26 Pages: 1247-1254

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Presentation] 全内部反射-光退色後蛍光回復法による液液界面上のローダミンBの側方拡散係数の顕微測定2013

    • Author(s)
      篠森直樹, 塚原 聡
    • Organizer
      日本化学会第93春季年会
    • Place of Presentation
      立命館大学(草津市)
    • Related Report
      2012 Final Research Report
  • [Presentation] 全内部反射-光退色後蛍光回復法による液液界面上のローダミンBの側方拡散係数の顕微測定2013

    • Author(s)
      篠森直樹
    • Organizer
      日本化学会第93春季年会
    • Place of Presentation
      立命館大学(滋賀県草津市)
    • Related Report
      2012 Annual Research Report
  • [Presentation] 液液界面現象のin situ顕微分光計測2012

    • Author(s)
      塚原 聡
    • Organizer
      分析技術研究会2011 年度研究発表会
    • Place of Presentation
      パナソニックリゾート大阪(吹田市)
    • Year and Date
      2012-03-08
    • Related Report
      2012 Final Research Report
  • [Presentation] 水性二相界面での酵素による単一DNA分子の分解反応のin situ 顕微蛍光測定2012

    • Author(s)
      片山 和也, 塚原 聡
    • Organizer
      第31回溶媒抽出討論会
    • Place of Presentation
      石川県文教会館(金沢市)
    • Related Report
      2012 Final Research Report
  • [Presentation] Aerosol OT/水/イソオクタン系におけるトリプシンの溶媒抽出と構造変化2012

    • Author(s)
      豊川裕也, 塚原 聡, 岡本泰明, 藤原照文
    • Organizer
      第31回溶媒抽出討論会
    • Place of Presentation
      石川県文教会館(金沢市)
    • Related Report
      2012 Final Research Report
  • [Presentation] 水溶液中における単一金ナノ粒子の動的挙動の顕微測定2012

    • Author(s)
      田中真実, 塚原 聡
    • Organizer
      日本分析化学会第61 年会
    • Place of Presentation
      金沢大学(金沢市)
    • Related Report
      2012 Final Research Report
  • [Presentation] 液液界面へのレーザー光照射による金ナノ粒子の生成挙動のin situ 顕微測定とその解析2012

    • Author(s)
      橋本佑介, 塚原 聡, 岡本泰明, 石坂 昌司, 藤原照文
    • Organizer
      日本分析化学会第61年会
    • Place of Presentation
      金沢大学(金沢市)
    • Related Report
      2012 Final Research Report
  • [Presentation] 水性二相界面での酵素による単一DNA分子の分解反応のin situ 顕微蛍光測定2012

    • Author(s)
      片山 和也
    • Organizer
      第31回溶媒抽出討論会
    • Place of Presentation
      石川県文教会館(石川県金沢市)
    • Related Report
      2012 Annual Research Report
  • [Presentation] Aerosol OT/水/イソオクタン系におけるトリプシンの溶媒抽出と構造変化2012

    • Author(s)
      豊川 裕也
    • Organizer
      第31回溶媒抽出討論会
    • Place of Presentation
      石川県文教会館(石川県金沢市)
    • Related Report
      2012 Annual Research Report
  • [Presentation] 水溶液中における単一金ナノ粒子の動的挙動の顕微測定2012

    • Author(s)
      田中 真実
    • Organizer
      日本分析化学会第61年会
    • Place of Presentation
      金沢大学(石川県金沢市)
    • Related Report
      2012 Annual Research Report
  • [Presentation] 液液界面へのレーザー光照射による金ナノ粒子の生成挙動のin situ顕微測定とその解析2012

    • Author(s)
      橋本 佑介
    • Organizer
      日本分析化学会第61年会
    • Place of Presentation
      金沢大学(石川県金沢市)
    • Related Report
      2012 Annual Research Report
  • [Presentation] 液液界面における特異反応のリアルタイム顕微計測2011

    • Author(s)
      塚原 聡
    • Organizer
      日本分析化学会近畿支部第二回講演会
    • Place of Presentation
      大阪科学技術センター(大阪市)
    • Year and Date
      2011-12-09
    • Related Report
      2012 Final Research Report
  • [Presentation] 液液界面に形成したテトラフェニルポルフィナトマンガン(III)の会合体のin situ顕微分光測定と解析2011

    • Author(s)
      八坂栄次, 塚原 聡, 岡本泰明, 藤原照文
    • Organizer
      日本分析化学会中国四国支部 平成22年度広島地区分析技術講演会
    • Place of Presentation
      広島大学学士会館レセプションホール(東広島市)
    • Year and Date
      2011-03-08
    • Related Report
      2012 Final Research Report
  • [Presentation] 液液界面に形成したテトラフェニルポルフィナトマンガン(III)の会合体のin situ顕微分光測定と解析2011

    • Author(s)
      八坂栄次
    • Organizer
      日本分析化学会中国四国支部広島地区講演会
    • Place of Presentation
      広島大学学士会館(東広島市)
    • Year and Date
      2011-03-08
    • Related Report
      2010 Annual Research Report
  • [Presentation] In Situ Microscope Measurements of Dynamic Behaviors of Substances Existing at and near Liquid/Liquid Interfaces2011

    • Author(s)
      塚原 聡
    • Organizer
      第21回日本MRS学術シンポジウム
    • Place of Presentation
      波止場会館(横浜市)
    • Related Report
      2012 Final Research Report
  • [Presentation] Aerosol OT/水/イソオクタン系における水溶性色素の抽出と水相の塩濃度の関係2011

    • Author(s)
      豊川裕也, 塚原 聡, 岡本泰明, 藤原照文
    • Organizer
      第30回溶媒抽出討論会
    • Place of Presentation
      フェニックスシーガイアリゾート(宮崎市)
    • Related Report
      2012 Final Research Report
  • [Presentation] 液液界面現象のin situ顕微分光計測の現状と夢"2011

    • Author(s)
      塚原 聡
    • Organizer
      第30回溶媒抽出討論会, フェニックスシーガイアリゾート(宮崎市)
    • Place of Presentation
      宮崎市
    • Related Report
      2012 Final Research Report
  • [Presentation] In Situ Microscope Measurements of Dynamic Behaviors of Substances Existing at and near Liquid/Liquid Interfaces2011

    • Author(s)
      塚原 聡
    • Organizer
      第21回日本MRS学術シンポジウム
    • Place of Presentation
      波止場会館(神奈川県横浜市)
    • Related Report
      2011 Annual Research Report
    • Invited
  • [Presentation] Aerosol OT/水/イソオクタン系における水溶性色素の抽出と水相の塩濃度の関係2011

    • Author(s)
      豊川 裕也
    • Organizer
      第30回溶媒抽出討論会
    • Place of Presentation
      フェニックスシーガイアリゾート(宮崎県宮崎市)
    • Related Report
      2011 Annual Research Report
  • [Presentation] 液液界面現象のin situ顕微分光計測の現状と夢2011

    • Author(s)
      塚原 聡
    • Organizer
      第30回溶媒抽出討論会
    • Place of Presentation
      フェニックスシーガイアリゾート(宮崎県宮崎市)
    • Related Report
      2011 Annual Research Report
    • Invited
  • [Presentation] 液液界面における特異反応のin situ 高時空間分解計測への挑戦2010

    • Author(s)
      塚原 聡
    • Organizer
      日本分析化学会中国四国支部 周南地区講演会
    • Place of Presentation
      徳曹会館(周南市)
    • Year and Date
      2010-12-03
    • Related Report
      2012 Final Research Report
  • [Presentation] 液液界面における特異反応のin situ高時空間分解計測への挑戦2010

    • Author(s)
      塚原聡
    • Organizer
      日本分析化学会中国四国支部周南地区講演会
    • Place of Presentation
      徳曹会館(周南市)(招待講演)
    • Year and Date
      2010-12-03
    • Related Report
      2010 Annual Research Report
  • [Presentation] 液液界面に吸着したマンガン(III)ポルフィリン錯体の会合体のin situ顕微分光測定と解析2010

    • Author(s)
      八坂栄次
    • Organizer
      日本分析化学会第59年会
    • Place of Presentation
      東北大学川内北キャンパス(仙台市)
    • Year and Date
      2010-09-15
    • Related Report
      2010 Annual Research Report
  • [Presentation] 液液界面に吸着したマンガン(III)ポルフィリン錯体の会合体のin situ 顕微分光測定と解析2010

    • Author(s)
      八坂栄次, 塚原 聡, 岡本泰明, 藤原照文
    • Organizer
      日本分析化学会第59年会
    • Place of Presentation
      東北大学川内北キャンパス(仙台市)
    • Related Report
      2012 Final Research Report
  • [Presentation] 液液界面現象のin situ顕微分光計測

    • Author(s)
      塚原 聡
    • Organizer
      分析技術研究会 2011年度研究発表会
    • Place of Presentation
      パナソニックリゾート大阪(大阪府吹田市)
    • Related Report
      2011 Annual Research Report
    • Invited
  • [Presentation] 液液界面における特異反応のリアルタイム顕微計測

    • Author(s)
      塚原 聡
    • Organizer
      日本分析化学会近畿支部第二回講演会
    • Place of Presentation
      大阪科学技術センター(大阪府大阪市)
    • Related Report
      2011 Annual Research Report
    • Invited
  • [Remarks] 大阪大学大学院理学研究科化学専攻 分析化学研究室

    • URL

      http://www.chem.sci.osaka-u.ac.jp/lab/tsukahara/

    • Related Report
      2012 Final Research Report
  • [Remarks] 大阪大学大学院理学研究科化学専攻 分析化学研究室

    • URL

      http://www.chem.sci.osaka-u.ac.jp/lab/tsukahara/

    • Related Report
      2012 Annual Research Report
  • [Remarks] 大阪大学大学院理学研究科化学専攻 分析化学研究室

    • URL

      http://www.chem.sci.osaka-u.ac.jp/lab/tsukahara/index.html

    • Related Report
      2011 Annual Research Report

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Published: 2010-08-23   Modified: 2019-07-29  

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