Stable-Isotope Probing of plastics film for investigation of surface microbial community

• Project/Area Number: 22350067
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Environmental chemistry
• Research Institution: University of Tsukuba
• Principal Investigator: NAKAJIMA Toshiaki 筑波大学, 生命環境系, 筑波大学・生命環境系・ (80241777)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥18,070,000 (Direct Cost: ¥13,900,000、Indirect Cost: ¥4,170,000); Fiscal Year 2012: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2011: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000); Fiscal Year 2010: ¥8,710,000 (Direct Cost: ¥6,700,000、Indirect Cost: ¥2,010,000)
• Keywords: 生分解性物質 / 生分解性プラスチック / 安定同位体標識法 / モノマーリサイクル

Research Abstract

In this study, stable isotope probing of PHB film with 13C in order to explore the PHB degraders from different soil samples. The DNA Extracted from 13C labeled PHB film was subjected to ultracentrifugation. The resultant DNA was found labeled with 13C. It indicates that most of the microbes present on the surface of film might be PHB degraders. This 13C DNA was analyzed through 16S rRNA gene based T-RFLP and clonal analysis to identify different microbial communities. The results indicated the presence of different microbial communities on the surface of PHB films.

Research Products

• [Journal Article] Rapid and simple colorimetric assay for detecting the enzymatic degradation of biodegradable plastic films (2013)
  • Author(s): Y. Shinozaki, T. Watanabe, T. Nakajima-Kambe, H. K. Kitamoto
  • Journal Title: J. Biosci. Bioeng Volume: 115 Pages: 111-114
  • Peer Reviewed
• [Journal Article] Enzymatic degradation of polyester films by a cutinase-like enzyme from Pseudozyma antarctica: surface plasmon resonance and atomic force microscopy study (2013)
  • Author(s): Y. Shinozaki, Y. Kikkawa, S. Sato, T. Fukuoka, T. Watanabe, S. Yoshida, T. Nakajima-Kambe, H. K. Kitamoto
  • Journal Title: Appl. Microbiol. Biotechnol.
  • Peer Reviewed
• [Journal Article] Purification and properties of novel aliphatic-aromatic co-polyesters degrading enzymes from newly isolated Roseateles depolymerans strain TB-87 (2013)
  • Author(s): A. A. Shah, T. Eguchi, D. Mayumi, S. Kato, N. Shintani, N. R. Kamini, T. Nakajima-Kambe
  • Journal Title: Polym. Degrad. Stab Volume: 98 Pages: 609-618
  • Peer Reviewed
• [Journal Article] Biodegradable plastic-degrading enzyme from Pseudozyma antarctica: cloning, sequencing, and characterization. (2012)
  • Author(s): Y. Shinozaki, T. Morita, X. H. Cao, S. Yoshida, M. Koitabashi, T. Watanabe, K. Suzuki, Y. Sameshima-Yamashita, T. Nakajima-Kambe, T. Fujii, H. K. Kitamoto
  • Journal Title: Appl. Microbiol. Biotechnol.
  • Peer Reviewed
• [Journal Article] Purification, cloning and expression of an Aspergillus niger lipase for degradation of poly(lactic acid) and poly(epsilon-caprolactone). (2012)
  • Author(s): T. Nakajima-Kambe, N. G. Edwinoliver, H. Maeda, K. Thirunavukarasu, M. K. Gowthaman, K. Masaki, S. Mahalingam, N. R. Kamini
  • Journal Title: Polym. Degrad. Stab. Volume: 97 Pages: 139-144
  • Peer Reviewed
• [Journal Article] Purification and properties of novel aliphatic-aromatic co-polyesters degra ding enzymes from newly isolated Roseateles depolymerans strain TB-87 (2012)
  • Author(s): Shah, A. A.
  • Journal Title: Polymer Degradation and Stability Volume: 98 Issue: 2 Pages: 609-618
  • DOI: 10.1016/j.polymdegradstab.2012.11.013
  • Peer Reviewed
• [Journal Article] Purification, cloning and expression of an Aspergillus niger lipase for degradation of poly(lactic acid) and poly(epsilon-caprolactone) (2012)
  • Author(s): Nakajima-Kambe, T.
  • Journal Title: Polymer Degradation and Stability Volume: 97 Issue: 2 Pages: 139-144
  • DOI: 10.1016/j.polymdegradstab.2011.11.009
  • Peer Reviewed
• [Journal Article] Phyllosphere yeasts rapidly break down biodegradable plastics (2011)
  • Author(s): H. K. Kitamoto, Y. Shinozaki, X. H. Cao, T. Morita, M. Konishi, K. Tago, H. Kajiwara, M. Koitabashi, S. Yoshida, T. Watanabe, Y. Sameshima-Yamashita, T. Nakajima-Kambe, S. Tsushima
  • Journal Title: AMB Express Volume: 1 Pages: 44-44
  • Peer Reviewed
• [Journal Article] Phyllosphere yeasts rapidly break down biodegradable plastics (2011)
  • Author(s): Kitamoto, H.K
  • Journal Title: AMB Express Volume: 1 Issue: 1 Pages: 1-11
  • DOI: 10.1186/2191-0855-1-44
  • Peer Reviewed
• [Presentation] Enzymatic Degradation of Polyester Films by Cutinase-Like Enzyme from a Yeast Pseudozyma antarctica :a Surface Plasmon Resonance Analysis. (2012)
  • Author(s): Y. Shinozaki, Y. Kikkawa, S. Sato, T. Fukuoka, D. Kitamoto, T. Watanabe, T. Nakajima-Kambe
  • Organizer: The 9th SPSJ International Polymer Conference
  • Place of Presentation: 兵庫県神戸市
  • Year and Date: 2012-12-14
• [Presentation] Leptothrix sp. TB-71 と Roseateles depolymerans TB-87の共培養によるpoly(butylene succinate adipate)(PBSA) 分解速度の向上 (2012)
  • Author(s): 羽尾純一, 中島敏明, 野村暢彦, 内山裕夫
  • Organizer: 環境バイオテクノロジー学会2012年度大会
  • Place of Presentation: 京都府宇治市
  • Year and Date: 2012-06-25
• [Presentation] Purification and properties of novel aliphatic-aromatic copolyester degrading enzymes from newly isolated Roseateles depolymerans strain TB-87 (2012)
  • Author(s): A. A. Shah, T. Eguchi, F. Ichihashi, D. Mayumi, S. Kato, N. Shintani, T. Nakajima-Kambe
  • Organizer: The 1st Biotechnology World Congress
  • Place of Presentation: Dubai, UAE
  • Year and Date: 2012-02-14
• [Presentation] Stable-Isotope Probing of plastics film for investigation of surface microbial community. (2012)
  • Author(s): M. Ohshima, N. Nomura, H. Uchiyama, T. Nakajima-Kambe
  • Organizer: The 1st Biotechnology World Congress
  • Place of Presentation: Dubai, UAE
  • Year and Date: 2012-02-14
• [Presentation] Degradation of PBSA by PBSA depolymerase from Leptothrix sp.TB-71,and analysis of degradation products (2012)
  • Author(s): Y. Matsumoto, H. Uchiyama, N. Nomura, T. Nakajima-Kambe
  • Organizer: The 1st Biotechnology World Congress
  • Place of Presentation: Dubai, UAE
  • Year and Date: 2012-02-14
• [Presentation] Stable-Isotope Probing of plastics film for investigation of surface microbial community (2012)
  • Author(s): Ohshima, M.
  • Organizer: The 1st Biotechnology World Congress
  • Place of Presentation: Dubai, UAE
  • Year and Date: 2012-02-14
• [Presentation] Degradation of PBSA by PBSA depolymerase from Leptothrix sp.TB-71, and analysis of degradation products (2012)
  • Author(s): Matsumoto, Y.
  • Organizer: The 1st Biotechnology World Congress
  • Place of Presentation: Dubai, UAE
  • Year and Date: 2012-02-14
• [Presentation] Leptothrix sp. TB-71 とRoseateles depolymerans TB-87 の共培養によるpoly(butylene succinate adipate)(PBSA)分解速度の向上 (2012)
  • Author(s): 中島敏明
  • Organizer: 環境バイオテクノロジー学会 2012年度大会
  • Place of Presentation: 京都大学宇治キャンパス
• [Presentation] 埋設生分解性プラスチックの微生物群集構造と有用遺伝子の解析 (2011)
  • Author(s): 大嶋恵, 齋藤真理, 眞弓大介, 野村暢彦, 内山裕夫, 中島敏明
  • Organizer: 2011 年度土壌微生物学会大会
  • Place of Presentation: 宮城県大崎市
  • Year and Date: 2011-11-26
• [Presentation] 埋設生分解性プラスチックの微生物群集構造と有用遺伝子の解析 (2011)
  • Author(s): 大嶋恵
  • Organizer: 2011年度土壌微生物学会大会
  • Place of Presentation: シーガイア(宮城県大崎市)
  • Year and Date: 2011-11-26
• [Presentation] 埋設生分解性プラスチックの微生物群集構造と有用遺伝子の解析 (2011)
  • Author(s): 大嶋恵, 齋藤真理, 眞弓大介, 野村暢彦, 内山裕夫, 中島敏明
  • Organizer: 第27回日本微生物生態学会大会
  • Place of Presentation: 京都府京都市
  • Year and Date: 2011-10-08
• [Presentation] 埋設生分解性プラスチックの微生物群集構造と有用遺伝子の解析 (2011)
  • Author(s): 大嶋恵
  • Organizer: 第27回日本微生物生態学会大会
  • Place of Presentation: 京都大学(京都府京都市)
  • Year and Date: 2011-10-08
• [Presentation] SIP 法を用いた生分解性プラスチック分解菌の動態解析 (2011)
  • Author(s): 大嶋恵, 野村暢彦, 内山裕夫, 中島敏明
  • Organizer: 第60回高分子学会年次大会
  • Place of Presentation: 大阪府大阪市
  • Year and Date: 2011-05-26
• [Presentation] Leptothrix sp.TB-71 株由来 PBSA depolymerase の生産と分解機構の解明 (2011)
  • Author(s): 松本唯 , 中 島 敏 明
  • Organizer: 第60回高分子学会年次大会
  • Place of Presentation: 大阪府大阪市
  • Year and Date: 2011-05-26
• [Presentation] SIP法を用いた生分解性プラスチック分解菌の動態解析 (2011)
  • Author(s): 大嶋恵
  • Organizer: 第60回高分子学会年次大会
  • Place of Presentation: 大阪国際会議場(大阪府大阪市)
  • Year and Date: 2011-05-26
• [Presentation] 細菌由来 PBSA分解酵素の分解機構の解明. (2011)
  • Author(s): 松本唯, 中島敏明
  • Organizer: 高分子学会(グリーンケミストリー研究発表会)
  • Place of Presentation: 東京都千代田区
  • Year and Date: 2011-01-14
• [Presentation] 安定同位体ラベル法による生分解性プラスチック分解菌の動態解析 (2010)
  • Author(s): 齋藤真理, 野村暢彦, 内山裕夫, 中島敏明(2010)
  • Organizer: 日本微生物生態学会第26回大会
  • Place of Presentation: 茨城県つくば市
  • Year and Date: 2010-11-25
• [Presentation] 安定同位体ラベル法による生分解性プラスチック分解菌の動態解析 (2010)
  • Author(s): 齋藤真理
  • Organizer: 第26回日本微生物生態学会大会
  • Place of Presentation: 筑波大学(茨城県つくば市)
  • Year and Date: 2010-11-25
• [Book] 微生物酵素による生分解性プラスチックのモノマーリサイクル." リサイクル・廃棄物事典 (2012)
  • Author(s): 中島敏明
  • Total Pages: 503
  • Publisher: 産業調査会
• [Book] リサイクル・廃棄物事典 (2012)
  • Author(s): 中島敏明
  • Total Pages: 695
  • Publisher: 微生物酵素による生分解性プラスチックのモノマーリサイクル
• [Book] リサイクル・廃棄物事典(産業調査会) (2012)
  • Author(s): 中島敏明
  • Publisher: 微生物酵素による生分解性プラスチックのモノマーリサイクル(印刷中)
• [Book] 酵素利用技術大系NTS(小宮山眞編) (2010)
  • Author(s): 中島敏明
  • Publisher: ポリマーを分解する酵素
• [Remarks]
  • URL: http://researchmap.jp/nakajimatoshiaki
• [Patent(Industrial Property Rights)] 生分解性プラスチック分解酵素をコードする遺伝子の取得方法、それにより得られる新規遺伝子および酵素 (2011)
  • Inventor(s): 中島敏明
  • Industrial Property Rights Holder: 科学技術振興機構
  • Filing Date: 2011-01-28
• [Patent(Industrial Property Rights)] 新規ウレタン結合分解菌 (2010)
  • Inventor(s): 中島敏明
  • Industrial Property Rights Holder: 科学技術振興機構
  • Filing Date: 2010-12-08
  • Overseas
• [Patent(Industrial Property Rights)] 新規ウレタン結合分解菌 (2010)
  • Inventor(s): 中島敏明
  • Industrial Property Rights Holder: 科学技術振興機構
  • Filing Date: 2010-04-20
  • Overseas