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Supporting system for regenerative medicine by utilizing three-dimensional micro dynamic stimulations and active manipulations on the iPS cells

Research Project

Project/Area Number 22360093
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Dynamics/Control
Research InstitutionYamagata University

Principal Investigator

KOSAWADA Tadashi  山形大学, 理工学研究科, 教授 (10143083)

Co-Investigator(Renkei-kenkyūsha) FENG Zhonggang  山形大学, 理工学研究科, 准教授 (10332545)
GOTO Kaoru  山形大学, 医学部, 教授 (30234975)
Project Period (FY) 2010-04-01 – 2014-03-31
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥18,460,000 (Direct Cost: ¥14,200,000、Indirect Cost: ¥4,260,000)
Fiscal Year 2013: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Fiscal Year 2012: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2011: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2010: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000)
Keywords動的刺激 / 3次元刺激 / iPS細胞 / アクティブ力学操作 / 分化誘導 / 細胞増殖 / 振動ステージ / 振動アクチュエータ / 3次元アクチュエータ / 3次元振動ステージ / 3次元動的力学刺激 / 分化誘導促進 / 神経細胞 / 選択的力学刺激 / 3次元動的力学刺激 / マウスiPS細胞 / アクティブ力学作
Research Abstract

We have developed completely new small sized piezoelectric three-dimensional vibration stage and a micro vibration actuator, which enable us to enforce various micro dynamic stimulations upon the cultured living iPS cells colony as well as upon a single cell. The system, not like a conventional approach but based on the principle of mechanics, has an ability to support development of the regenerative medicine. The results suggest that utilization of appropriate dynamic stimulation may be a promising approach not only to accelerate iPS cells proliferation but also to improve differentiation efficiency of iPS cells into neurons as well as neuronal networking.

Report

(5 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Annual Research Report
  • 2011 Annual Research Report
  • 2010 Annual Research Report
  • Research Products

    (55 results)

All 2014 2013 2012 2011 2010 Other

All Journal Article (18 results) (of which Peer Reviewed: 14 results) Presentation (32 results) (of which Invited: 2 results) Book (2 results) Remarks (3 results)

  • [Journal Article] Analysis of the Contraction of Fibroblast-Collagen Gels and the Traction Force of Individual Cells by a Novel Elementary Structural Model2013

    • Author(s)
      Z. Feng, Y. Wagatsuma, S. Kobayashi, T. Kosawada, D. Sato, T. Nakamura, T. Kitajima, M. Umezu
    • Journal Title

      Proceedings of 35th Annual International Conference of the IEEE EMBS

      Pages: 6232-6235

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Analysis of the Contraction of Fibroblast-Collagen Gels and the Traction Force of Individual Cells by a Novel Elementary Structural Model2013

    • Author(s)
      Z. Feng, Y. Wagatsuma, S. Kobayashi, T. Kosawada, D. Sato, T. Nakamura, T. Kitajima, M. Umezu
    • Journal Title

      Proc. of 35th Annual International Conference of the IEEE EMBS

      Volume: 403 Pages: 6232-6235

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Non-invasive Stiffness Detection Method for Living Cell Nucleus by Using Piezoelectric Micro Sensor2012

    • Author(s)
      Ken-ichi Konno, Tadashi Kosawada, Yasushi Kaneyama, Hiroya Endo, Zhonggang Feng
    • Journal Title

      World Congress on Medical Physics and Biomedical Engineering

      Volume: 39 Pages: 290-293

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Novel Three-dimensional Micro Vibration Stage and Its Ability to Influence in Cellular Senescence2012

    • Author(s)
      Ken-ichi Konno, Tadashi Kosawada, Toru Ichita, Zhonggang Feng, Yasukazu Hozumi, Kaoru Goto
    • Journal Title

      World Congress on Medical Physics and Biomedical Engineering

      Volume: 39 Pages: 274-277

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] D&D2011 におけるダイナミクスと制御の研究動向2012

    • Author(s)
      小沢田 正, 他6名
    • Journal Title

      「7.細胞,組織,臓器のダイナミクスとその応用」, 日本機械学会論文集(C編)

      Volume: 78-789 Pages: 1306-1314

    • NAID

      130002051394

    • Related Report
      2013 Final Research Report
  • [Journal Article] Non-invasive Stiffness Detection Method for Living Cell Nucleus by Using Piezoelectric Micro Sensor2012

    • Author(s)
      Ken-ichi Konno, Tadashi Kosawada, Yasushi Kaneyama, Hiroya Endo, Zhonggang Feng
    • Journal Title

      IFMBE Proceedings

      Volume: 39 Pages: 290-293

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Novel Three-dimensional Micro Vibration Stage and Its Ability to Influence in Cellular Senescence2012

    • Author(s)
      Ken-ichi Konno, Tadashi Kosawada, Toru Ichita, Zhonggang Feng, Yasukazu Hozumi, Kaoru Goto
    • Journal Title

      IFMBE Proceedings

      Volume: 39 Pages: 274-277

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Non-invasive Stiffness Evaluation Method for Living Cellular Nucleus by Using Micro Piezoelectric Vibration Device2011

    • Author(s)
      Tadashi Kosawada, Ken-ichi Konno, Yasushi Kaneyama, Zhonggang Feng, Yasukazu Hozumi and Kaoru Goto
    • Journal Title

      International Symposium Physicochemical Field for Genetic Activities

      Pages: 38-38

    • Related Report
      2013 Final Research Report
  • [Journal Article] Non-invasive Stiffness Evaluation Method for Living Cellular Nucleus by Using Micro Piezoelectric Vibration Device2011

    • Author(s)
      T.Kosawada, K.Konno, Y.Kaneyama Z.Feng, Y.Hozumi, K.Goto
    • Journal Title

      Proc.International Symposium Physicochemical Field for Genetic Activities

      Pages: 38-38

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 動的力学刺激を用いたiPS細胞の培養コントロール2011

    • Author(s)
      阿部淳司, 今野健一, 市田徹, 工藤佳明, 小沢田正, 馮忠剛
    • Journal Title

      Proc.Dynamics & Design Conference 2011

      Volume: 11-2-204(CD-R0M) Pages: 6-6

    • Related Report
      2011 Annual Research Report
  • [Journal Article] ヒト骨芽細胞核の力学特性評価システムの開発2011

    • Author(s)
      金山寧, 今野健一, 遠藤洋也, 小沢田正, 馮忠剛
    • Journal Title

      Proc.Dynamics & Design Conference 2011

      Volume: 11-2-202(CD-ROM) Pages: 7-7

    • Related Report
      2011 Annual Research Report
  • [Journal Article] Dynamic actuation and sensing micro-device for mechanical response of cultured adhesive cells2010

    • Author(s)
      Ken-ichi Konno, Tadashi Kosawada, Masato Suzuki, Takeshi Nakamura, Zhonggang Feng, Yasukazu Hozumi, Kaoru Goto
    • Journal Title

      Microsystem Technologies

      Volume: Vol.16 Pages: 993-1000

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Three-dimensional Micro Vibration Stage and Its Application to Cell Culture2010

    • Author(s)
      Ken-ichi KONNO, Tadashi KOSAWADA, Ryota SATO, Zhonggang Feng, Yasukazu HOZUMI and Kaoru GOTO
    • Journal Title

      World Congress of Biomechanics 2010

      Volume: 31 Pages: 1409-1412

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Piezoelectric Micro Probe Device for Mechanical Stimulation and Its Detection for Living Cells2010

    • Author(s)
      Ken-ichi KONNO, Tadashi KOSAWADA, Takeshi NAKAMURA, Zhonggang Feng
    • Journal Title

      World Congress of Biomechanics 2010

      Volume: 31 Pages: 1413-1416

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Dynamic actuation and sensing micro device for mechanical response of cultured adhesive cells2010

    • Author(s)
      K.Konno, T.Kosawada, M.Suzuki, T.Nakamura, Z.Feng, Y.Hozumi, K.Goto
    • Journal Title

      Microsystem Technologies

      Volume: 16-6 Pages: 993-1000

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Viscoelastic characteristics of contracted collagen gels populated with rat fibroblasts or cardiomyocytes2010

    • Author(s)
      Z.Feng, D.Seya, T.Kitajima, T.Kosawada, T.Nakamura, M.Umezu
    • Journal Title

      Journal of Artificial Organs

      Volume: 13-3 Pages: 139-144

    • NAID

      10030807761

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Three-dimensional Micro Vibration Stage and Its Application to Cell Culture2010

    • Author(s)
      K.KONNO, T.KOSAWADA, R.SATO, Z.Feng, Y.HOZUMI, K.GOTO
    • Journal Title

      World Congress of Biomechanics 2010, IFMBE Proceedings

      Volume: 31 Pages: 1409-1412

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Piezoelectric Micro Probe Device for Mechanical Stimulation and Its Detection for Living Cells2010

    • Author(s)
      K.KONNO, T.KOSAWADA, T.NAKAMURA, Z.Feng
    • Journal Title

      World Congress of Biomechanics 2010, IFMBE Proceedings

      Volume: 31 Pages: 1413-1416

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Presentation] ヒト iPS 細胞の神経細胞への分化過程における動的力学刺激の影響評価2014

    • Author(s)
      真坂 洋平, 早坂 紘旗, 小沢田 正, 馮 忠剛
    • Organizer
      日本機械学会東北支部第49期総会
    • Place of Presentation
      東北大学工学部
    • Year and Date
      2014-03-14
    • Related Report
      2013 Final Research Report
  • [Presentation] 動的力学刺激によるマウスニューロンネットワークの3次元的形成への影響2014

    • Author(s)
      金子 暢生, 永田 哲平, 伊東 彬, 小沢田正, 長谷川 麗, 宇山 浩
    • Organizer
      日本機械学会東北支部第44回卒業研究発表講演会
    • Place of Presentation
      山形大学工学部
    • Year and Date
      2014-03-11
    • Related Report
      2013 Final Research Report
  • [Presentation] ヒト iPS 細胞の神経細胞への分化に及ぼす動的力学刺激の影響評価2014

    • Author(s)
      菊池 駿佑, 真坂 洋平, 早坂 紘旗, 小沢田 正
    • Organizer
      日本機械学会東北支部第44回卒業研究発表講演会
    • Place of Presentation
      山形大学工学部
    • Year and Date
      2014-03-11
    • Related Report
      2013 Final Research Report
  • [Presentation] iPS細胞の基礎と研究上のニーズ2014

    • Author(s)
      小沢田 正
    • Organizer
      iPS関連機器開発参入促進オープンセミナー
    • Place of Presentation
      仙台市 : TKPガーデンシティ仙台
    • Year and Date
      2014-01-17
    • Related Report
      2013 Final Research Report
    • Invited
  • [Presentation] iPS細胞の基礎 と 研究上のニーズ2014

    • Author(s)
      小沢田 正
    • Organizer
      iPS関連機器開発 参入促進オープンセミナー
    • Place of Presentation
      TKPガーデンシティ仙台
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] マウス神経細胞の3次元ネットワーク形成に及ぼす動的力学刺激の影響2013

    • Author(s)
      永田 哲平, 小沢田 正, 長谷川 麗, 宇山浩
    • Organizer
      日本機械学会東北支部第49期秋季講演会講演論文集
    • Place of Presentation
      岩手大学工学部
    • Year and Date
      2013-09-20
    • Related Report
      2013 Final Research Report
  • [Presentation] マウスiPS細胞の分化に及ぼす動的力学刺激の影響に関する研究2013

    • Author(s)
      小泉 智幸, 宇賀神 一也, 小沢田 正, 馮忠剛
    • Organizer
      日本機械学会Dynamics and Design Conference 2013, USB論文集
    • Place of Presentation
      九州産業大学
    • Year and Date
      2013-08-30
    • Related Report
      2013 Final Research Report
  • [Presentation] マウスiPS細胞の分化に及ぼす動的力学刺激の影響に関する研究2013

    • Author(s)
      小泉 智幸,宇賀神 一也,小沢田 正,馮 忠剛
    • Organizer
      Dynamics and Design Conference 2013
    • Place of Presentation
      九州産業大学
    • Related Report
      2013 Annual Research Report
  • [Presentation] ヒト骨芽細胞核の力学特性と細胞周期との関連に関する研究2013

    • Author(s)
      安食 亮介,遠藤 洋也,小沢田 正,馮 忠剛
    • Organizer
      Dynamics and Design Conference 2013
    • Place of Presentation
      九州産業大学
    • Related Report
      2013 Annual Research Report
  • [Presentation] マウス神経細胞の3次元ネットワーク形成に及ぼす動的力学刺激の影響2013

    • Author(s)
      永田 哲平,小沢田 正,長谷川 麗,宇山 浩
    • Organizer
      日本機械学会東北支部第49期秋季講演会
    • Place of Presentation
      岩手大学
    • Related Report
      2013 Annual Research Report
  • [Presentation] 超小型3次元振動ステージの細胞培養への応用2012

    • Author(s)
      後藤 敬成, 今野 健一, 小沢田 正, 馮 忠剛, 八月朔日 泰和, 後藤 薫
    • Organizer
      日本機械学会東北支部第48期秋季講演会講演論文集
    • Place of Presentation
      八戸高専
    • Year and Date
      2012-09-22
    • Related Report
      2013 Final Research Report
  • [Presentation] マウスiPS細胞の分化過程における力学刺激の影響評価2012

    • Author(s)
      小泉 智幸, 会田 和広, 今野 健一, 馮 忠剛, 小沢田 正
    • Organizer
      日本機械学会Dynamics and Design Conference 2012, USB論文集
    • Place of Presentation
      慶応義塾大学日吉キャンパス
    • Year and Date
      2012-09-19
    • Related Report
      2013 Final Research Report
  • [Presentation] Non-invasive Stiffness Detection Method for Living Cell Nucleus by Using Piezoelectric Micro Sensor2012

    • Author(s)
      Ken-ichi Konno, Tadashi Kosawada, Yasushi Kaneyama, Hiroya Endo, Zhonggang Feng
    • Organizer
      World Congress on Medical Physics and Biomedical Engineering
    • Place of Presentation
      Beijing・China
    • Related Report
      2012 Annual Research Report
  • [Presentation] Novel Three-dimensional Micro Vibration Stage and Its Ability to Influence in Cellular Senescence2012

    • Author(s)
      Ken-ichi Konno, Tadashi Kosawada, Toru Ichita, Zhonggang Feng, Yasukazu Hozumi, Kaoru Goto
    • Organizer
      World Congress on Medical Physics and Biomedical Engineering
    • Place of Presentation
      Beijing・China
    • Related Report
      2012 Annual Research Report
  • [Presentation] ヒト骨芽細胞核の形状および力学特性評価法の開発2012

    • Author(s)
      遠藤 洋也,金山 寧,今野 健一,馮 忠剛, 小沢田 正
    • Organizer
      日本機械学会Dynamics and Design Conference 2012
    • Place of Presentation
      慶応大(神奈川県)
    • Related Report
      2012 Annual Research Report
  • [Presentation] マウスiPS細胞の分化過程における力学刺激の影響評価2012

    • Author(s)
      小泉 智幸,会田 和広,今野 健一,馮 忠剛,小沢田 正
    • Organizer
      日本機械学会Dynamics and Design Conference 2012
    • Place of Presentation
      慶応大(神奈川県)
    • Related Report
      2012 Annual Research Report
  • [Presentation] マウスiPS細胞の分化過程における力学特性の評価2011

    • Author(s)
      小泉 智幸, 会田 和広, 今野 健一, 小沢田 正, 馮 忠剛
    • Organizer
      日本機械学会東北支部第47期秋季講演会講演論文集
    • Place of Presentation
      山形大学工学部
    • Year and Date
      2011-09-22
    • Related Report
      2013 Final Research Report
  • [Presentation] 超小型3次元振動ステージの開発と細胞培養コントロールへの応用2011

    • Author(s)
      市田 徹, 今野 健一, 小沢田 正, 馮忠剛, 八月朔日 泰和, 後藤 薫
    • Organizer
      日本機械学会東北支部第47期秋季講演会講演論文集
    • Place of Presentation
      山形大学工学部
    • Year and Date
      2011-09-22
    • Related Report
      2013 Final Research Report
  • [Presentation] 長時間細胞操作用培養環境維持装置の開発2011

    • Author(s)
      工藤 佳明, 今野 健一, 小沢田 正, 馮 忠剛
    • Organizer
      日本機械学会東北支部第47期秋季講演会講演論文集
    • Place of Presentation
      山形大学工学部
    • Year and Date
      2011-09-22
    • Related Report
      2013 Final Research Report
  • [Presentation] マウスiPS細胞の分化過程における力学特性の評価2011

    • Author(s)
      小泉智幸, 会田和広, 今野健一, 小沢田正, 馮忠剛
    • Organizer
      日本機械学会東北支部第47期秋季講演会
    • Place of Presentation
      山形大学工学部
    • Year and Date
      2011-09-22
    • Related Report
      2011 Annual Research Report
  • [Presentation] 超小型3次元振動ステージの開発と細胞培養コントロールへの応用2011

    • Author(s)
      市田徹, 今野健一, 小沢田正, 馮忠剛, 八月朔日泰和, 後藤薫
    • Organizer
      日本機械学会東北支部第47期秋季講演会
    • Place of Presentation
      山形大学工学部
    • Year and Date
      2011-09-22
    • Related Report
      2011 Annual Research Report
  • [Presentation] 長時間細胞操作用培養環境維持装置の開発2011

    • Author(s)
      工藤佳明, 今野健一, 小沢田正, 馮忠剛
    • Organizer
      日本機械学会東北支部第47期秋季講演会
    • Place of Presentation
      山形大学工学部
    • Year and Date
      2011-09-22
    • Related Report
      2011 Annual Research Report
  • [Presentation] ヒト骨芽細胞核の力学特性評価法の開発2011

    • Author(s)
      遠藤洋也, 金山寧, 今野健一, 小沢田正, 馮忠剛
    • Organizer
      日本機械学会東北支部第47期秋季講演会
    • Place of Presentation
      山形大学工学部
    • Year and Date
      2011-09-22
    • Related Report
      2011 Annual Research Report
  • [Presentation] 動的力学刺激を用いたiPS細胞の培養コントロール2011

    • Author(s)
      阿部 淳司, 今野 健一, 市田 徹, 工藤佳明, 小沢田 正, 馮 忠剛
    • Organizer
      Dynamics and Design Conference 2011, CD-ROM 論文集204
    • Place of Presentation
      高知工科大学
    • Year and Date
      2011-09-05
    • Related Report
      2013 Final Research Report
  • [Presentation] iPS細胞の分化過程における力学特性の評価2010

    • Author(s)
      会田 和広, 今野 健一, 阿部 淳司, 中村 健, 小沢田 正
    • Organizer
      Dynamics and Design Conference 2011
    • Place of Presentation
      同志社大学京田辺キャンパス
    • Year and Date
      2010-09-15
    • Related Report
      2013 Final Research Report
  • [Presentation] 超小型3次元振動ステージの開発と細胞培養への応用2010

    • Author(s)
      市田 徹 , 今野 健一 , 金山 寧 , 佐藤 亮太 , 小沢田 正, 馮 忠剛, 八月朔日 泰和 , 後藤 薫
    • Organizer
      Dynamics and Design Conference 2011
    • Place of Presentation
      同志社大学京田辺キャンパス
    • Year and Date
      2010-09-15
    • Related Report
      2013 Final Research Report
  • [Presentation] ips細胞の分化過程における力学特性の評価2010

    • Author(s)
      会田和広, 今野健一, 小沢田正馮忠剛, 他2名
    • Organizer
      Dynamics and Design Conference 2010
    • Place of Presentation
      京田辺市, 同志社大学
    • Year and Date
      2010-09-15
    • Related Report
      2010 Annual Research Report
  • [Presentation] 超小型3次元振動ステージの開発と細胞培養への応用2010

    • Author(s)
      市田徹, 今野健一, 小沢田正馮忠剛, 後藤薫, 他3名
    • Organizer
      Dynamics and Design Conference 2010
    • Place of Presentation
      京田辺市, 同志社大学
    • Year and Date
      2010-09-15
    • Related Report
      2010 Annual Research Report
  • [Presentation] 細胞内膜動輸送における小胞の変形シミュレーション2010

    • Author(s)
      石澤尚大, 小沢田正
    • Organizer
      日本シミュレーション学会大会
    • Place of Presentation
      米沢市, 山形大学
    • Year and Date
      2010-06-20
    • Related Report
      2010 Annual Research Report
  • [Presentation] 血管内皮細胞貫通チャネルの形成解析及びチャネルを介した物質輸送シミュレーション2010

    • Author(s)
      石澤尚大, 小沢田正, 五代聡也
    • Organizer
      日本シミュレーション学会大会
    • Place of Presentation
      米沢市, 山形大学
    • Year and Date
      2010-06-20
    • Related Report
      2010 Annual Research Report
  • [Presentation] 超小型3次元振動ステージの細胞培養への応用

    • Author(s)
      後藤 敬成,今野 健一,小沢田 正,馮 忠剛,八月朔日 泰和,後藤 薫
    • Organizer
      日本機械学会東北支部第48期秋季講演会
    • Place of Presentation
      八戸高専(青森県)
    • Related Report
      2012 Annual Research Report
  • [Presentation] 細胞内小胞の形状解析及び物質輸送に及ぼすダイアフラムの影響

    • Author(s)
      小池智哉,齋藤悠介,小沢田正
    • Organizer
      日本機械学会東北支部第48期秋季講演会
    • Place of Presentation
      八戸高専(青森県)
    • Related Report
      2012 Annual Research Report
  • [Book] ≪最新≫動物細胞培養の手法と細胞死・増殖不良・細胞変異を防止する技術, 「2-5-1 : 動的力学刺激によるヒトiPS細胞の培養・分化制御法」2014

    • Author(s)
      小沢田 正
    • Publisher
      技術情報協会
    • Related Report
      2013 Final Research Report
  • [Book] 動物細胞培養の手法と細胞死・増殖不良・細胞変異を防止する技術2014

    • Author(s)
      小沢田 正
    • Total Pages
      600
    • Publisher
      技術情報協会
    • Related Report
      2013 Annual Research Report
  • [Remarks] 小沢田[バイオメカニクス]研究室 ホームページ

    • URL

      http://kosawada_lab.yz.yamagata-u.ac.jp

    • Related Report
      2012 Annual Research Report
  • [Remarks]

    • URL

      http://kosawada_lab.yz.yamagata-u.ac.jp

    • Related Report
      2011 Annual Research Report
  • [Remarks]

    • URL

      http://kosawada_lab.yz.yamagatau.ac.jp

    • Related Report
      2010 Annual Research Report

URL: 

Published: 2010-08-23   Modified: 2019-07-29  

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