| Budget Amount *help |
¥18,330,000 (Direct Cost: ¥14,100,000、Indirect Cost: ¥4,230,000)
Fiscal Year 2012: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
Fiscal Year 2011: ¥6,110,000 (Direct Cost: ¥4,700,000、Indirect Cost: ¥1,410,000)
Fiscal Year 2010: ¥8,970,000 (Direct Cost: ¥6,900,000、Indirect Cost: ¥2,070,000)
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| Research Abstract |
Identification of anaerobic protozoa in an UASB pilot plant treating domestic municipal sewage was carried out by 18S rRNA gene cloning to clear how many species existed in. Abundances according to each species were measured by microscopic direct counts throughout three years. Metopus contortus, Metopus palaeformis, Trimyema compressum were predominance as a protozoan in the UASB when present in large numbers. Total cell number of the protozoa shifted with changes in temperature of the sewage, and up to 11x103 cells/mL (fall) and became 0 cells/mL (spring). FISH analysis to Metopus contortus revealed that hydrogen utilizing methanogen present at a level of 104 cells in one protozoan cell as their symbiotic bacteria. Metopus sp played a important role to reduce MLSS in the UASB by their predation.
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