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Construction of functional peptide ligands toward peptide biochips

Research Project

Project/Area Number 22360341
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Biofunction/Bioprocess
Research InstitutionTokyo Institute of Technology

Principal Investigator

MIHARA Hisakazu  東京工業大学, 生命理工学研究科, 教授 (30183966)

Co-Investigator(Kenkyū-buntansha) TSUTSUMI Hiroshi  東京工業大学, 大学院生命理工学研究科, 助教 (70398105)
Project Period (FY) 2010-04-01 – 2013-03-31
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥19,630,000 (Direct Cost: ¥15,100,000、Indirect Cost: ¥4,530,000)
Fiscal Year 2012: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2011: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2010: ¥10,400,000 (Direct Cost: ¥8,000,000、Indirect Cost: ¥2,400,000)
Keywordsバイオチップ / ペプチド / 捕捉剤
Research Abstract

To develop peptide biochips with high-throughput functions, a variety of peptide ligands were constructed by means of chemical and phage-display methods. It is successfully demonstrated that functional peptides were synthesized by a phage-display method using a beta-loop peptide library, peptide-displaying phage-chips were constructed, a variety of sugar-conjugated peptides (with gold-nanoparticles) were prepared, and peptides functioning to cells were also constructed.

Report

(4 results)
  • 2013 Final Research Report ( PDF )
  • 2012 Annual Research Report
  • 2011 Annual Research Report
  • 2010 Annual Research Report
  • Research Products

    (17 results)

All 2013 2012 2011 2010

All Journal Article (9 results) (of which Peer Reviewed: 8 results) Presentation (7 results) Book (1 results)

  • [Journal Article] A Monosaccharide-modified Peptide Phage Library for Screening of Ligands to Carbohydrate-binding Proteins2013

    • Author(s)
      K. Arai, H. Tsutsumi, H. Mihara
    • Journal Title

      Bioorg. Med. Chem. Lett.

      Volume: 23 Pages: 4940-4943

    • Related Report
      2013 Final Research Report 2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Gold Nanoparticles Conjugated with Monosaccharide-modified Peptide for Lectin Detection2012

    • Author(s)
      H. Tsutsumi, H. Ohkusa, H. Park, T.Takahashi, H. Yuasa, H. Mihara
    • Journal Title

      Bioorg. Med. Chem. Lett.

      Volume: 22 Pages: 6825-6827

    • Related Report
      2013 Final Research Report 2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A Novel Peptide Array Using a Phage Display System for Protein Detection2011

    • Author(s)
      T. Sawada, K. Ishiguro, T. Takahashi, H. Mihara
    • Journal Title

      Chem. Lett.

      Volume: 40 Pages: 508-509

    • NAID

      10028242940

    • Related Report
      2013 Final Research Report 2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A Novelβ-Loop Scaffold of Phage-Displayed Peptides for Highly Specific Affinities2011

    • Author(s)
      T. Sawada, K. Ishiguro, T. Takahashi, H. Mihara
    • Journal Title

      Mol. BioSyst.

      Volume: 7 Pages: 2558-2562

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Cell Fingerprint Patterns Using Designedα-Helical Peptides to Screen for Cell-specific Toxicity2011

    • Author(s)
      K. Usui, T. Kakiyama, K.-Y. Tomizaki, M. Mie, E. Kobatake, H. Mihara
    • Journal Title

      Bioorg. Med. Chem. Lett.

      Volume: 21 Pages: 6281-6284

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] A Novel β-Loop Scaffold of Phage-Displayed Peptides for Highly Specific Affinities2011

    • Author(s)
      Toshiki Sawada
    • Journal Title

      Mol. BioSyst.

      Volume: 7 Pages: 2558-2562

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Cell Fingerprint Patterns Using Designed α-Helical Peptides to Screen for Cell-specific Toxicity2011

    • Author(s)
      Kenji Usui
    • Journal Title

      Bioorg. Med. Chem. Lett.

      Volume: 21 Pages: 6281-6284

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Protein-Protein Interaction and Screening : Array-based Techniques for Screening Disease-Associated Biomarkers in Predictive/Early Diagnostics2010

    • Author(s)
      K.-Y. Tomizaki, K. Usui, H. Mihara
    • Journal Title

      FEBS Journal

      Volume: 277 Pages: 1996-2005

    • Related Report
      2013 Final Research Report 2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] デザインペプチドを用いたプロテインアレイと細胞アレイ2010

    • Author(s)
      臼井健二、富崎欣也、三原久和
    • Journal Title

      化学フロンティア22,生命現象を理解する分子ツール

      Pages: 79-87

    • Related Report
      2010 Annual Research Report
  • [Presentation] マンノース修飾ペプチドを提示したファージライブラリの構築とコンカナバリンA結合性ペプチドの探索2012

    • Author(s)
      新井佳菜子,大倉裕通,堤浩,三原久和
    • Organizer
      第49回ペプチド討論会
    • Place of Presentation
      鹿児島
    • Related Report
      2013 Final Research Report
  • [Presentation] Designed peptide libraries for cell analyses2012

    • Author(s)
      Hisakazu Mihara
    • Organizer
      2012 International Symposium on Advanced Biological Engineering
    • Place of Presentation
      中国
    • Related Report
      2013 Final Research Report
  • [Presentation] CONSTRUCTION OF A PHAGE LIBRARY DISPLAYING MANNOSE-MODIFIED PEPTIDES AND SCREENING OF BINDING PEPTIDES TO CONCANAVALIN A2012

    • Author(s)
      Kanako Arai, Hiromichi Okura, Hiroshi Tsutsumi, Hisakazu Mihara
    • Organizer
      第49回日本ペプチド討論会
    • Place of Presentation
      鹿児島
    • Related Report
      2012 Annual Research Report
  • [Presentation] Designed Peptide Libraries for Cell Analyses2012

    • Author(s)
      Hisakazu Mihara
    • Organizer
      2012 International Symposium on Advanced Biological Engineering
    • Place of Presentation
      Guilin, China
    • Related Report
      2012 Annual Research Report
  • [Presentation] αへリックスペプチドを結合した金ナノ粒子の細胞導入活性とガン細胞応用2011

    • Author(s)
      Park Hyejin,高橋剛,堤浩,三原久和
    • Organizer
      第48回ペプチド討論会
    • Place of Presentation
      札幌
    • Related Report
      2013 Final Research Report 2011 Annual Research Report
  • [Presentation] レクチンに対する結合多様性を有する単糖導入ペプチド修飾金ナノ粒子の創製2011

    • Author(s)
      大草寛之,朴恵珍,高橋剛,堤浩,湯浅英哉,三原久和
    • Organizer
      第5回バイオ関連化学シンポジウム
    • Place of Presentation
      つくば
    • Related Report
      2013 Final Research Report 2011 Annual Research Report
  • [Presentation] Designed peptide libraries for protein and cell analyses2010

    • Author(s)
      Hisakazu Mihara
    • Organizer
      5th International Peptide Symposium
    • Place of Presentation
      京都
    • Related Report
      2013 Final Research Report 2010 Annual Research Report
  • [Book] デザインペプチドを用いたプロテインアレイと細胞アレイ 化学フロンティア22,生命現象を理解する分子ツール2010

    • Author(s)
      臼井健二,富崎欣也,三原久和
    • Total Pages
      181
    • Publisher
      化学同人
    • Related Report
      2013 Final Research Report

URL: 

Published: 2010-08-23   Modified: 2019-07-29  

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