Analysis of transcriptional regulation cascade of organic solvent tolerant yeasts and its application

• Project/Area Number: 22360342
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Biofunction/Bioprocess
• Research Institution: Kyoto University
• Principal Investigator: UEDA Mitsuyoshi 京都大学, 大学院・農学研究科, 教授 (90183201)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥19,500,000 (Direct Cost: ¥15,000,000、Indirect Cost: ¥4,500,000); Fiscal Year 2012: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000); Fiscal Year 2011: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000); Fiscal Year 2010: ¥8,320,000 (Direct Cost: ¥6,400,000、Indirect Cost: ¥1,920,000)
• Keywords: 有機溶媒耐性 / 転写制御因子 / ストレス応答 / ABC トランスポーター / PDR1 変異 / 薬剤耐性 / 情報伝達システム / 相互作用 / ABCトランスポーター / PDR1変異 / 生体触媒 / 転写因子 / 分子育種 / 細胞表層 / 相互作用因子

Research Abstract

As solvents for the chemical reactions are usually highly toxic to the catalytic carrier organism, organic solvent tolerance is necessary for effective bioproduction. Specific expression of the identified genes would improve the tolerance to particular organic solvents for practical use. We demonstrated the PDR1 R821S mutantis tolerant to hydrophilic organic solvent, DMSO, as well as hydrophobic organic solvents. Furthermore, we identified and discriminated genes encoding ABC transporters and cell wall proteins involved in tolerance to hydrophobic or hydrophilic organic solvents.

Research Products

• [Journal Article] ABC transporters and cell wall proteins involved in organic solvent tolerance in Saccharomyces cergvisiae (2013)
  • Author(s): Nao Nishida, Naoki Ozato, Ken Matsui, Kouichi Kuroda, Mitsuvoshi Ueda.
  • Journal Title: Journal of biotechnology Volume: 165 Issue: 2 Pages: 145-152
  • DOI: 10.1016/j.jbiotec.2013.03.003
  • Peer Reviewed
• [Journal Article] Direct fermentation of newspaper after laccase-treatment using yeast codisplaying endoglucanase, cellobiohydrolase, and β-glucosidase (2012)
  • Author(s): Nakanishi A, Kuroda K, Ueda M
  • Journal Title: Renewable Energy Volume: 44 Pages: 199-205
  • DOI: 10.1016/j.renene.2012.01.078
  • Peer Reviewed
• [Journal Article] ABC transporters and cell wall proteins involved in organic solvent tolerance in Saccharomyces cerevisiae (2012)
  • Author(s): N. Nishida
  • Journal Title: J. Biotechnol. Volume: 未定
  • Peer Reviewed
• [Journal Article] Synthesis of functional dipeptide, carnosine, from nonprotected amino acids using carnosinase -displaying yeast cells (2010)
  • Author(s): C. Inaba, S. Higuchi, H. Morisaka, K. Kuroda, M. Ueda
  • Journal Title: Appl. Microbiol. Biotechnol. Volume: 86 Issue: 6 Pages: 1895-1902
  • DOI: 10.1007/s00253-009-2396-7
  • Peer Reviewed
• [Journal Article] Enhancement of beta-glucosidase activity on the cell-surface of sake yeast by disruption of SED1 (2010)
  • Author(s): A. Kotaka, H. Sahara, K. Kuroda, A. Kondo, M. Ueda, Y. Hata, A. Kotaka, H. Sahara, K. Kuroda, A. Kondo, M. Ueda, Y. Hata
  • Journal Title: J. Biosci. Bioeng Volume: 109 Issue: 5 Pages: 442-446
  • DOI: 10.1016/j.jbiosc.2009.11.003
  • Peer Reviewed
• [Journal Article] 分子デイスプレイ法による革新的有機溶媒内酵素反応の開拓 (2010)
  • Author(s): 植田充美
  • Journal Title: 薬学雑誌 Volume: 130 Pages: 1479-1485
  • Peer Reviewed
• [Journal Article] Synthesis of functional dipeptide, carnosine, from nonprotected amino acids using carnosinase-displaying yeast cells (2010)
  • Author(s): C.Inaba, et al.
  • Journal Title: Appl.Microbiol.Biotechnol. Volume: 86 Pages: 1895-1902
  • Peer Reviewed
• [Journal Article] Enhancement of beta-glucosidase activity on the cell-surface of sake yeast by disruption of SED1 (2010)
  • Author(s): A.Kotaka, et al.
  • Journal Title: J.Biosci.Bioeng. Volume: 109 Pages: 442-446
  • Peer Reviewed
• [Journal Article] 分子ディスプレイ法による革新的有機溶媒内酵素反応の開拓 (2010)
  • Author(s): 植田充美
  • Journal Title: 薬学雑誌 Volume: 130 Pages: 1479-1485
  • NAID: 130000451698
  • Peer Reviewed
• [Presentation] ABC transporters and cell wall proteins involved in yeast organic solvent tolerance (2012)
  • Author(s): N. Nshida et al.
  • Organizer: 15th European Congress of Biotechnology
  • Place of Presentation: Istanbul, Turkey
  • Year and Date: 2012-09-23
• [Presentation] 酵母の有機溶媒耐性に関与するタンパク質の決定 (2012)
  • Author(s): 西田奈央
  • Organizer: 日本生物工学会若手会
  • Place of Presentation: 仙台
• [Presentation] Functional analysis of Pdr1p in multidrug resistance and organic-solvent tolerance in yeast (2011)
  • Author(s): N. Nshida et al.
  • Organizer: 2011 American Society for Biochemistry and Molecular Biology Annual Meeting
  • Place of Presentation: Washington DC, USA
  • Year and Date: 2011-04-11
• [Presentation] Functional analysis of Pdrlp in multidrug resistance and organic-solvent tolerance in yeast (2011)
  • Author(s): N.Nishida, et al
  • Organizer: 2011 American Society for Biochemistry and Molecular Biology annual meeting
  • Place of Presentation: Washington DC, USA
  • Year and Date: 2011-04-11
• [Presentation] Molecular analysis of XBD in Pdr1p mutants leading to multidrug resistance and organic-solvent tolerance in yeast Saccharomyces cerevisiae (2010)
  • Author(s): N. Nishida et al.
  • Organizer: Pacjfichem 2010
  • Place of Presentation: Hawaii, USA
  • Year and Date: 2010-12-18
• [Presentation] Molecular analysis of XBD in Pdr1p mutants leading to multidrug resistance and organic-solvent tolerance in yeast Saccharomyces cerevisiae (2010)
  • Author(s): N.Nishida, et al.
  • Organizer: Pacifichem 2010
  • Place of Presentation: USA, Hawaii
  • Year and Date: 2010-12-18
• [Remarks]
  • URL: http://www/tenko.kais.kyoto-u.ac.jp/