| Budget Amount *help |
¥19,500,000 (Direct Cost: ¥15,000,000、Indirect Cost: ¥4,500,000)
Fiscal Year 2012: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2011: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2010: ¥8,320,000 (Direct Cost: ¥6,400,000、Indirect Cost: ¥1,920,000)
|
|---|
| Research Abstract |
As solvents for the chemical reactions are usually highly toxic to the catalytic carrier organism, organic solvent tolerance is necessary for effective bioproduction. Specific expression of the identified genes would improve the tolerance to particular organic solvents for practical use. We demonstrated the PDR1 R821S mutantis tolerant to hydrophilic organic solvent, DMSO, as well as hydrophobic organic solvents. Furthermore, we identified and discriminated genes encoding ABC transporters and cell wall proteins involved in tolerance to hydrophobic or hydrophilic organic solvents.
|
