Development of basic technology to simplify biocatalytic processes for the production of chemicals

• Project/Area Number: 22360343
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Biofunction/Bioprocess
• Research Institution: Osaka University
• Principal Investigator: OHTAKE Hisao 大阪大学, 工学研究科, 教授 (10127483)
• Co-Investigator(Kenkyū-buntansha): HONDA Khosuke 大阪大学, 工学研究科, 准教授 (90403162)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥18,980,000 (Direct Cost: ¥14,600,000、Indirect Cost: ¥4,380,000); Fiscal Year 2012: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2011: ¥7,020,000 (Direct Cost: ¥5,400,000、Indirect Cost: ¥1,620,000); Fiscal Year 2010: ¥7,670,000 (Direct Cost: ¥5,900,000、Indirect Cost: ¥1,770,000)
• Keywords: 耐熱性酵素 / 生体触媒 / バイオプロセス / 加熱処理 / 合成代謝工学 / 耐熱酵素 / 微生物 / 有用物質生産 / 好熱菌 / バイオプロダクション / 疎水性細菌

Research Abstract

We have developed a simple, new bioproduction platform which is potentially applicable to the synthesis of specialty chemicals.The newly developed process has many potential advantages: (i) whatever target metabolites can be specifically produced; (ii) enzyme purification is not required; (iii) no by-product is formed; (iv) bioconversions can be performed using a simple reactor without elaborate process control; and (v) reaction can be prevented from microbial contamination

Research Products

• [Journal Article] Direct conversion of glucose to malate by synthetic metabolic engineering. (2013)
  • Author(s): Ye, X.
  • Journal Title: J. Biotechnol. Volume: 164 Pages: 34-40
  • Peer Reviewed
• [Journal Article] Development of continuous bioconversion system using thermophilic whole-cell (2013)
  • Author(s): Ninh, P.H.
  • Journal Title: Appl. Environ. Microbiol. Volume: 79 Pages: 1996-2001
  • Peer Reviewed
• [Journal Article] Bioproduction of Vanillin using an Organic Solvent-tolerant (2012)
  • Author(s): N. Wangthamrongwit, K. Klinsakul, A. S. Vangnai, J. Wongkongkatep, P. Inprakhon, K. Honda, H. Ohtake, J. Kato and T. Pongtharangkul
  • Journal Title: Brevibacillus agri 13. Appl. Microbiol. Biotechnol. Volume: 93 Pages: 555-563
  • Peer Reviewed
• [Journal Article] Construction of membrane-anchoring fusion protein of Thermococcus kodakaraensis KOD1glycerol kinase and its application to repetitive batchwise reactions (2012)
  • Author(s): E. Restiawaty, K. Honda, K. Okano, R. Hirota, T. Omasa, A. Kuroda, H. Ohtake
  • Journal Title: J. Biosc. Bioeng. Volume: 113 Pages: 521-525
  • Peer Reviewed
• [Journal Article] Synthetic metabolic engineering-A novel, simple technology for designing a chimeric metabolic pathway- (2012)
  • Author(s): Ye, X., Honda, K., Sakai, T., Okano, K., Omasa, T., Hirota , R., Kuroda, A., and Ohtake, H.
  • Journal Title: Microb. Cell Fact. Volume: 11 Pages: 212-212
  • Peer Reviewed
• [Journal Article] Analyses of both the alkB gene transcriptional start site and alkB promoter inducing properties in Rhodococcus sp (2011)
  • Author(s): Cappelletti, M., Fedi, S., Frascari, D., Ohtake, H., Turner, R.J., and Zannoni, D
  • Journal Title: BCP1 as grown on n-alkanes. Appl. Environ. Microbiol. Volume: 77 Pages: 1619-1627
  • Peer Reviewed
• [Journal Article] Feasibility of thermophilic adenosine triphosphate-regeneration system using Thermus thermophilus polyphosphate kinase (2011)
  • Author(s): Elvi Restiawaty, ほか
  • Journal Title: Process Biochemistry Volume: 46 Issue: 9 Pages: 1747-1752
  • DOI: 10.1016/j.procbio.2011.05.021
  • Peer Reviewed
• [Journal Article] Enhancement of recombinant enzyme activity in cpxA-deficient mutant of Escherichia coli. (2010)
  • Author(s): Zhou, Y., Minami, T., Honda, K., Omasa, T., Ohtake, H.
  • Journal Title: J.Biosci.Bioeng. Volume: 110 Pages: 403-407
  • NAID: 110007880136
  • Peer Reviewed
• [Journal Article] Production of 2-deoxyribose 5-phosphate from fructose to demonstrate a potential or artificial bio-synthetic pathway using thermophilic enzymes. (2010)
  • Author(s): Honda, K., Maya, S., Omasa, T., Hirota, R., Kuroda, A., Ohtake, H.
  • Journal Title: J.Biotechnol. Volume: 148 Pages: 204-207
  • Peer Reviewed
• [Journal Article] Electron microscopic analysis of heat-induced leakage of polyphosphate from a phoU mutant of Escherichia coli. (2010)
  • Author(s): Kashihara, H., Kang, B.M., Omasa, T., Honda, K., Sameshima, Y., Kuroda, A., Ohtake, H.
  • Journal Title: Biosci.Biotechnol.Biochem. Volume: 74 Pages: 865-868
  • NAID: 10027554272
  • Peer Reviewed
• [Presentation] 合成代謝工学による人工ピルビン酸酸化経路の構築 (2013)
  • Author(s): 本田孝祐、今川貴志、Ye Xiaoting、岡野憲司、大竹久夫
  • Organizer: 日本農芸化学会2013 年度大会
  • Place of Presentation: 仙台
  • Year and Date: 2013-03-26
• [Presentation] Synthetic metabolic engineering -A novel, simple technology for designing a chimeric metabolic pathway- (2012)
  • Author(s): 本田孝祐、Ye Xiaoting、岡野憲司、大竹久夫
  • Organizer: Society of Biotechnology, Japan, International Symposium on Green Growth
  • Place of Presentation: 神戸市
  • Year and Date: 2012-10-26
• [Presentation] Construction of a chimeric glycolysis pathway by synthetic metabolic engineering (2012)
  • Author(s): 本田孝祐、Ye Xiaoting、岡野憲司、大竹久夫
  • Organizer: IBS2012
  • Place of Presentation: 大邱、韓国
  • Year and Date: 2012-09-18
• [Presentation] Thermococcus kodakarensis KOD1由来malic enzymeの補酵素要求性改変と合成代謝工学経路への利用 (2012)
  • Author(s): 森本有美
  • Organizer: 第64回日本生物工学会大会
  • Place of Presentation: 神戸国際会議場
• [Presentation] 「合成代謝工学」バイオプロダクションのための新しいパラダイムの構築について (2011)
  • Author(s): 大竹久夫、本田孝祐、岡野憲司、黒田章夫、加藤純一、岩田英之
  • Organizer: 第63回日本生物工学会大会
  • Place of Presentation: 東京農工大学
  • Year and Date: 2011-09-26
• [Presentation] Synthetic Metabolic Engineering -the proof of concept (2011)
  • Author(s): 本田孝祐、廣田隆一、黒田章夫、大竹久夫
  • Organizer: International Union of Microbiological Societies 2011 Congress (IUMS 2011)
  • Place of Presentation: Sapporo.
  • Year and Date: 2011-09-20
• [Presentation] 耐熱性Malic enzymeを用いたリンゴ酸の生産 (2010)
  • Author(s): 木村龍、叶暁〓、本田孝祐、大政健史、大竹久夫
  • Organizer: 第62回日本生物工学会大会
  • Place of Presentation: 宮崎市
  • Year and Date: 2010-10-29