Efficient production of virus-like particles using insect cells
Project/Area Number |
22360345
|
Research Category |
Grant-in-Aid for Scientific Research (B)
|
Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Biofunction/Bioprocess
|
Research Institution | Kobe University |
Principal Investigator |
YAMAJI Hideki 神戸大学, 大学院・工学研究科, 教授 (40283874)
|
Project Period (FY) |
2010 – 2012
|
Project Status |
Completed (Fiscal Year 2012)
|
Budget Amount *help |
¥19,760,000 (Direct Cost: ¥15,200,000、Indirect Cost: ¥4,560,000)
Fiscal Year 2012: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2011: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2010: ¥10,270,000 (Direct Cost: ¥7,900,000、Indirect Cost: ¥2,370,000)
|
Keywords | バイオ生産プロセス / 昆虫細胞 / 組換えタンパク質生産 / ウイルスタンパク質 / ウイルス様粒子 / ワクチン |
Research Abstract |
Virus-like particles (VLPs) are composed of one or several recombinant viral surface proteins that spontaneously assemble into particulate structures similar to authentic virus particles or naturally occurring subviral particles. A high yield of a secretory form of flavivirus VLPs was successfully produced by lepidopteran insect cells engineered to coexpress the premembrane (prM) and envelope (E) proteins. This study demonstrated that recombinant insect cells offer a promising approach to the high-level production of VLPs for use as vaccines and diagnostic antigens.
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Report
(4 results)
Research Products
(25 results)