Efficient production of virus-like particles using insect cells

• Project/Area Number: 22360345
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Biofunction/Bioprocess
• Research Institution: Kobe University
• Principal Investigator: YAMAJI Hideki 神戸大学, 大学院・工学研究科, 教授 (40283874)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥19,760,000 (Direct Cost: ¥15,200,000、Indirect Cost: ¥4,560,000); Fiscal Year 2012: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2011: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2010: ¥10,270,000 (Direct Cost: ¥7,900,000、Indirect Cost: ¥2,370,000)
• Keywords: バイオ生産プロセス / 昆虫細胞 / 組換えタンパク質生産 / ウイルスタンパク質 / ウイルス様粒子 / ワクチン

Research Abstract

Virus-like particles (VLPs) are composed of one or several recombinant viral surface proteins that spontaneously assemble into particulate structures similar to authentic virus particles or naturally occurring subviral particles. A high yield of a secretory form of flavivirus VLPs was successfully produced by lepidopteran insect cells engineered to coexpress the premembrane (prM) and envelope (E) proteins. This study demonstrated that recombinant insect cells offer a promising approach to the high-level production of VLPs for use as vaccines and diagnostic antigens.

Research Products

• [Journal Article] Production of Japanese encephalitis virus-like particles in insect cells (2013)
  • Author(s): Hideki Yamaji, Eiji Konishi
  • Journal Title: Bioengineered Volume: (in press) Issue: 6 Pages: 438-442
  • DOI: 10.4161/bioe.24514
  • Peer Reviewed
• [Journal Article] Efficient production of Japanese encephalitis virus-like particles by recombinant lepidopteran insect cells (2013)
  • Author(s): Hideki Yamaji, Masataka Nakamura, Miwa Kuwahara, Yusuke Takahashi, Tomohisa Katsuda, Eiji Konishi
  • Journal Title: Applied Microbiology and Biotechnology Volume: 97 Issue: 3 Pages: 1071-1079
  • DOI: 10.1007/s00253-012-4371-y
  • NAID: 120005464744
  • Peer Reviewed
• [Journal Article] Production of Japanese encephalitis virus-like particles using the baculovirus-insect cell system (2012)
  • Author(s): Hideki Yamaji, Maiko Segawa, Masataka Nakamura, Tomohisa Katsuda, Miwa Kuwahara, Eiji Konishi
  • Journal Title: Journal of Bioscience and Bioengineering Volume: 114 Issue: 6 Pages: 657-662
  • DOI: 10.1016/j.jbiosc.2012.06.012
  • NAID: 110009578162
  • Peer Reviewed
• [Journal Article] 昆虫細胞を用いたウイルス様粒子の生産 (2012)
  • Author(s): 山地秀樹
  • Journal Title: PHARM TECH JAPAN Volume: 28 Pages: 2503-2508
  • URL: http://www.jiho.co.jp/shop/list/detail/tabid/272/catid/75/pdid/92354/Default.aspx
• [Presentation] Production and purification of Japanese encephalitis virus-like particles from recombinant insect cells (2012)
  • Author(s): Yuri Hotta, Azusa Minamitani, Kazuma Narita, Tomohisa Katsuda, Hideki Yamaji
  • Organizer: The 25th Annual and International Meeting of the Japanese Association for Animal Cell Technology (JAACT2012)
  • Place of Presentation: 名古屋
  • Year and Date: 2012-11-30
• [Presentation] Secretory production of virus-like particles by recombinant insect cells (2012)
  • Author(s): Hideki Yamaji, Miwa Kuwahara, Eiji Konishi
  • Organizer: The 15th European Congress on Biotechnology of the European Federation of Biotechnology (ECB15)
  • Place of Presentation: Istanbul, Turkey
  • Year and Date: 2012-09-26
• [Presentation] 昆虫細胞-バキュロウイルス系によるウイルス様粒子の生産 (2012)
  • Author(s): 山地秀樹,瀬川麻衣子,中村匡崇,勝田知尚
  • Organizer: 化学工学会第77 年会
  • Place of Presentation: 東京
  • Year and Date: 2012-03-15
• [Presentation] 昆虫細胞-バキュロウイルス系によるウイルス様粒子の生産 (2012)
  • Author(s): 山地秀樹, 瀬川麻衣子, 中村匡崇, 勝田知尚
  • Organizer: 化学工学会第77年会
  • Place of Presentation: 工学院大学(東京都)
  • Year and Date: 2012-03-15
• [Presentation] 昆虫細胞を用いたウイルス様粒子ワクチンの生産 (2012)
  • Author(s): 山地秀樹
  • Organizer: JBA バイオエンジニアリング研究会講演会
  • Place of Presentation: 東京
  • Year and Date: 2012-01-20
• [Presentation] Secretory production of virus-like particles by recombinant insect cells (2012)
  • Author(s): Hideki Yamaji
  • Organizer: 15th European Congress on Biotechnology (ECB15)
  • Place of Presentation: Istanbul, Turkey
• [Presentation] Production and purification of Japanese encephalitis virus-like particles from recombinant insect cells (2012)
  • Author(s): Hideki Yamaji
  • Organizer: 25th Annual and International Meeting of the Japanese Association for Animal Cell Technology (JAACT2012)
  • Place of Presentation: 名古屋
• [Presentation] 昆虫細胞によるウイルス様粒子生産に及ぼす培養条件の検討 (2011)
  • Author(s): 成田和馬,堀田有里,南谷梓,永菅尚,勝田知尚,山地秀樹
  • Organizer: 化学工学会第43回秋季大会
  • Place of Presentation: 名古屋
  • Year and Date: 2011-09-14
• [Presentation] 昆虫細胞によるウイルス様粒子生産に及ぼす培養条件の検討 (2011)
  • Author(s): 成田和馬, 堀田有里, 南谷梓, 永菅尚, 勝田知尚, 山地秀樹
  • Organizer: 化学工学会第43回秋季大会
  • Place of Presentation: 名古屋工業大学(愛知県)
  • Year and Date: 2011-09-14
• [Presentation] Evaluation of extracellular subviral particles of dengue type 2 virus produced by insect cells for use as vaccine and diagnostic antigens (2011)
  • Author(s): Miwa Kuwahara, Hideki Yamaji, Eiji Konishi
  • Organizer: IUMS (International Union of Microbiological Societies) 2011 Sapporo Congress
  • Place of Presentation: 札幌
  • Year and Date: 2011-09-13
• [Presentation] Efficient production of extracellular subviral particles of Japanese encephalitis virus by recombinant insect cells (2011)
  • Author(s): Hideki Yamaji, Takashi Nagasuga, Yusuke Takahashi, Masataka Nakamura, Tomohisa Katsuda, Miwa Kuwahara, Eiji Konishi
  • Organizer: IUMS (International Union of Microbiological Societies) 2011 Sapporo Congress
  • Place of Presentation: 札幌
  • Year and Date: 2011-09-13
• [Presentation] Efficient production of extracellular subviral particles of Japanese encephalitis virus by recombinant insect cells (2011)
  • Author(s): H.Yamaji, T.Nagasuga, Y.Takahashi, M.Nakamura, T.Katsuda, M.Kuwahara, E.Konishi
  • Organizer: IUMS (International Union of Microbiological Societies) 2011 Sapporo Congress
  • Place of Presentation: 札幌コンベンションセンター(北海道)
  • Year and Date: 2011-09-13
• [Presentation] Evaluation of extracellular subviral particles of dengue type 2 virus produced by insect cells for use as vaccine and diagnostic antigens (2011)
  • Author(s): M.Kuwahara, H.Yamaji, E.Konishi
  • Organizer: IUMS (International Union of Microbiological Societies) 2011 Sapporo Congress
  • Place of Presentation: 札幌コンベンションセンター(北海道)
  • Year and Date: 2011-09-13
• [Presentation] Secretory production of virus-like particles from recombinant insect cells (2011)
  • Author(s): Hideki Yamaji, Takashi Nagasuga, Yusuke Takahashi, Masataka Nakamura, Tomohisa Katsuda, Miwa Kuwahara, Eiji Konishi
  • Organizer: ESACT (European Society for Animal Cell Technology) 2011 Meeting
  • Place of Presentation: Vienna, Austria
  • Year and Date: 2011-05-16
• [Presentation] Secretory production of virus-like particles by recombinant insect cells (2011)
  • Author(s): H.Yamaji, T.Nagasuga, Y.Takahashi, M.Nakamura, T.Katsuda, M.Kuwahara, E.Konishi
  • Organizer: ESACT (European Society for Animal Cell Technology) 2011 Meeting in Vienna
  • Place of Presentation: Vienna Hofburg Congress Centre, Vienna, Austria
  • Year and Date: 2011-05-16
• [Presentation] Production of Japanese encephalitis virus-like particles in insect cell expression systems (2010)
  • Author(s): Hideki Yamaji, Takashi Nagasuga, Yusuke Takahashi, Masataka Nakamura, Tomohisa Katsuda, Miwa Kuwahara, Eiji Konishi
  • Organizer: 13th Asia Pacific Confederation of Chemical Engineering Congress(APCChE 2010)
  • Place of Presentation: Taipei, Taiwan
  • Year and Date: 2010-10-06
• [Presentation] Production of Japanese encephalitis virus-like particles in insect cell expression systems (2010)
  • Author(s): H.Yamaji, T.Nagasuga, Y.Takahashi, M.Nakamura, T.Katsuda, M.Kuwahara, E.Konishi
  • Organizer: The 13th Asia Pacific Confederation of Chemical Engineering Congress (APCChE 2010)
  • Place of Presentation: Howard International House, Taipei, (Taiwan)
  • Year and Date: 2010-10-06
• [Presentation] 培養昆虫細胞を用いた有用物質生産 (2010)
  • Author(s): 山地秀樹
  • Organizer: 化学工学会第42回秋季大会
  • Place of Presentation: 京都
  • Year and Date: 2010-09-07
• [Presentation] 培養昆虫細胞を用いた有用物質生産 (2010)
  • Author(s): 山地秀樹
  • Organizer: 化学工学会第42回秋季大会
  • Place of Presentation: 同志社大学(京都府)
  • Year and Date: 2010-09-07
• [Presentation] Production of Japanese encephalitis virus-like particles using insect cells (2010)
  • Author(s): Takashi Nagasuga, Yusuke Takahashi, Masataka Nakamura, Tomohisa Katsuda, Hideki Yamaji
  • Organizer: The 23rd Annual and International Meeting of the Japanese Association for Animal Cell Technology (JAACT2010)
  • Place of Presentation: 札幌
  • Year and Date: 2010-09-03
• [Presentation] Production of Japanese encephalitis virus-like particles using insect cells (2010)
  • Author(s): T.Nagasuga, Y.Takahashi, M.Nakamura, T.Katsuda, H.Yamaji
  • Organizer: The 23rd Annual and International Meeting of the Japanese Association for Animal Cell Technology (JAACT2010)
  • Place of Presentation: 北海道大学学術交流会館(北海道)
  • Year and Date: 2010-09-03