Cell-free display technology for optimization of antibody drugs

• Project/Area Number: 22360351
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Biofunction/Bioprocess
• Research Institution: Keio University
• Principal Investigator: DOI Nobuhide 慶應義塾大学, 理工学部, 准教授 (50327673)
• Co-Investigator(Kenkyū-buntansha): YANAGAWA Hiroshi 慶應義塾大学, 理工学部, 訪問教授 (40327672)
• Co-Investigator(Renkei-kenkyūsha): MATSUSHIMA Koji 東京大学, 大学院・医学系研究科, 教授 (50222427) ; MISUMI Shogo 熊本大学, 医学薬学研究部, 准教授 (40264311)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥19,240,000 (Direct Cost: ¥14,800,000、Indirect Cost: ¥4,440,000); Fiscal Year 2012: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000); Fiscal Year 2011: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000); Fiscal Year 2010: ¥7,670,000 (Direct Cost: ¥5,900,000、Indirect Cost: ¥1,770,000)
• Keywords: ウイルス / バイオテクノロジー / 免疫学 / 蛋白質 / 進化

Research Abstract

In this study, we performed in vitro evolution of a humanized single-chain antibody against a cyclic peptide that mimics the extracellular loop-2 of CCR5,which is a key target of inhibitory drugs for the entry of HIV (Human Immunodeficiency Virus), by usi

Research Products

• [Journal Article] In vitro selection of Fab fragments by mRNA display and gene-linking emulsion PCR (2012)
  • Author(s): Sumida, T., Yanagawa, H., Doi, N
  • Journal Title: J. Nucleic Acids Volume: 2012 Pages: 371379-371379
  • Peer Reviewed
• [Journal Article] DNA display selection of peptide ligands for a full-length human G protein-coupled receptor on CHO-K1 cells (2012)
  • Author(s): Doi,N.,Yamakawa,N.,Matsumoto,H.,Yamamoto,Y.,Nagano,T.,Matsumura,N.,Horisawa,K.,Yanagawa,H
  • Journal Title: PLoS ONE Volume: 7
  • Peer Reviewed
• [Journal Article] In vitro selection of Fab fragments by mRNA display and gene-linking emulsion PCR (2012)
  • Author(s): Sumida, T., Yanagawa, H., Doi, N.
  • Journal Title: J. Nucleic Acids Volume: 2012 Pages: 371379-371379
  • DOI: 10.1155/2012/371379
  • Peer Reviewed
• [Journal Article] mRNA display selection of an optimized MDM2-binding peptide that potently inhibits MDM2-p53 interaction (2011)
  • Author(s): Shiheido, H., Takashima, H., Doi, N., Yanagawa, H
  • Journal Title: PLoS ONE Volume: 6
  • Peer Reviewed
• [Journal Article] Comparison of the frequency of functional SH3 domains with different limited sets of amino acids using mRNA display (2011)
  • Author(s): Tanaka, J., Yanagawa, H., Doi, N
  • Journal Title: PLoS ONE Volume: 6
  • Peer Reviewed
• [Journal Article] DNA display selection of peptide ligands for a full-length human G protein-coupled receptor on CHO-K1 cells (2011)
  • Author(s): Doi,N.,Yamakawa,N.,Matsumoto,H.,Yamamoto,Y.,Nagano,T.,Matsumura,N.,Horisawa,K.,Yanagawa,H.
  • Journal Title: PLoS ONE Volume: 7 Issue: 1 Pages: 1-8
  • DOI: 10.1371/journal.pone.0030084
  • Peer Reviewed
• [Journal Article] mRNA display selection of an optimized MDM2-binding peptide that potently inhibits MDM2-p53 interaction (2011)
  • Author(s): Shiheido, H., Takashima, H., Doi, N., Yanagawa, H.
  • Journal Title: PLoS ONE Volume: 6 Issue: 3 Pages: 1-9
  • DOI: 10.1371/journal.pone.0017898
  • Peer Reviewed
• [Journal Article] Comparison of the frequency of functional SH3 domains with different limited sets of amino acids using mRNA display. (2011)
  • Author(s): Tanaka, J.
  • Journal Title: PLoS ONE Volume: 6 Pages: 1-8
  • Peer Reviewed
• [Journal Article] Comparative characteriza-tion of random-sequence proteins consisting of 5, 12 and 20 kinds of amino acids (2010)
  • Author(s): Tanaka, J., Doi, N., Takashima, H., Yanagawa, H
  • Journal Title: Protein Sci Volume: 19 Pages: 786-795
  • Peer Reviewed
• [Journal Article] mRNA display selection of a high-affinity, Bcl-XL-specific binding peptide (2010)
  • Author(s): Matsumura, N., Tsuji, T., Sumida, T., Kokubo, M., Onimaru, M., Doi, N., Takashima, H., Miyamoto-Sato, E., Yanagawa, H
  • Journal Title: FASEB J Volume: 24 Pages: 2201-2210
  • Peer Reviewed
• [Journal Article] mRNA display selection of a high-affinity, BcI-X_L-specific binding peptide. (2010)
  • Author(s): Matsumura, N.
  • Journal Title: FASEB J. Volume: 24 Pages: 2201-2210
  • Peer Reviewed
• [Journal Article] Comparative characterization of random-sequence proteins consisting of 5, 12 and 20 kinds of amino acids. (2010)
  • Author(s): Tanaka, J.
  • Journal Title: Protein Sci. Volume: 19 Pages: 786-795
  • Peer Reviewed
• [Presentation] In vitro virus (IVV)法を用いた人工癌幹細胞の接着を阻害する一本鎖抗体の選択 (2012)
  • Author(s): 田畠典子, 佐久間裕子, 清水孝恒, 佐谷秀行, 土居信英, 柳川弘志
  • Organizer: 第35回日本分子生物学会年会
  • Place of Presentation: 福岡
• [Presentation] 高親和性抗CCR5-本鎖抗体の中立的浮動による試験管内進化 (2011)
  • Author(s): 佐藤亮太
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2011-12-13
• [Presentation] CD44とxCTの相互作用を阻害する抗CD44v-本鎖抗体の試験管内選択 (2011)
  • Author(s): 梅澤洋貴
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2011-12-13
• [Presentation] 高親和性抗CCR5一本鎖抗体の中立的浮動による試験管内進化 (2011)
  • Author(s): 佐藤亮太,土居信英,三隅将吾,庄司省三,松島綱治,横地祥司,柳川弘志
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: 横浜
• [Presentation] CD44とxCTの相互作用を阻害する抗CD44v一本鎖抗体の試験管内選択 (2011)
  • Author(s): 梅澤洋貴,田畠典子,佐久間裕子,竹中悠馬,松本佳宣,土居信英,永 野 修 ,佐 谷 秀 行 ,柳川弘志
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: 横浜
• [Presentation] HIVの侵入を阻害する抗CCR5一本鎖抗体の試験管内進化 (2010)
  • Author(s): 佐藤亮太
  • Organizer: 第33回日本分子生物学会年会
  • Place of Presentation: 神戸
  • Year and Date: 2010-12-07
• [Presentation] In vitro display methods for heterodimeric proteins using in vitro compartmentaliza-tion (2010)
  • Author(s): Sumida, T., Yanagawa, H., Doi, N
  • Organizer: Pacifichem 2010
  • Place of Presentation: Honolulu
• [Presentation] In vitro selection of functional SH3 domains with reduced amino-acid alphabets (2010)
  • Author(s): Tanaka, J., Yanagawa, H., Doi, N.
  • Organizer: Pacifichem 2010
  • Place of Presentation: Honolulu
• [Presentation] HIV の侵入を阻害する抗 CCR5 一本鎖抗体の試験管内進化 (2010)
  • Author(s): 佐藤亮太,土居信英,三隅将吾,松島綱治,横地祥司,柳川弘志
  • Organizer: 第33回日本分子生物学会年会
  • Place of Presentation: 神戸
• [Presentation] In vitro virus (IVV)法を用いた人工癌幹細胞の接着を阻害する一本鎖抗体の選択
  • Author(s): 田畠典子
  • Organizer: 第35回日本分子生物学会年会
  • Place of Presentation: 福岡
• [Book] 次世代医薬開発に向けた抗体工学の最前線 (2012)
  • Author(s): 土居信英
  • Publisher: シーエムシー出版
• [Book] 現代生物科学入門9:合成生物学 (2010)
  • Author(s): 柳川弘志,土居信英
  • Publisher: 岩波書店
• [Book] 現代生物科学入門9:合成生物学 (2010)
  • Author(s): 土居信英
  • Total Pages: 220
  • Publisher: 岩波書店
• [Remarks]
  • URL: https://sites.google.com/site/biomoleng12/home