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Reverse genetics and recombination

Research Project

Project/Area Number 22370001
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Genetics/Genome dynamics
Research InstitutionUniversity of Shizuoka

Principal Investigator

IIDA Shigeru  静岡県立大学, 大学院・食品栄養環境科学研究院, 客員教授 (30012777)

Co-Investigator(Kenkyū-buntansha) JOHZUKA Yasuyo (HISATOMI Yasuyo)  静岡県立大学, 大学院・薬学研究科, 博士研究員 (60517887)
KINOSHITA Tetsu  奈良先端科学技術大学院大学, バイオサイエンス研究科, 研究チーム長 (60342630)
ISHIZAKI Kimitsune  京都大学, 大学院・生命科学研究科, 助教 (00452293)
Co-Investigator(Renkei-kenkyūsha) KOBAYASHI Hirokazu  静岡県立大学, 大学院・食品栄養環境科学研究院, 教授 (80170348)
NOGUCHI Hiroshi  静岡県立大学, 薬学部, 教授 (60126141)
WATANABE Kenji  静岡県立大学, 薬学部, 准教授 (50360938)
JOHZUKA Yasuyo (HISATOMI Yasuyo)  静岡県立大学, 大学院・食品栄養環境科学研究院, 客員研究員 (60517887)
Project Period (FY) 2010 – 2012
Project Status Completed (Fiscal Year 2012)
Budget Amount *help
¥18,850,000 (Direct Cost: ¥14,500,000、Indirect Cost: ¥4,350,000)
Fiscal Year 2012: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2011: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2010: ¥10,010,000 (Direct Cost: ¥7,700,000、Indirect Cost: ¥2,310,000)
Keywords植物 / 分子遺伝 / 逆遺伝学 / 遺伝子 / 突然変異 / バイオテクノロジー / ゲノム / 遺伝学 / 組換え機構
Research Abstract

To facilitate reverse genetic approaches, which include homologous recombination-mediated gene targeting using positive-negative selection and gene tagging employing endogenous nDart1-related DNA transposons, we were attempting to elucidate recombination mechanisms and their regulation mechanisms associated with gene targeting and tagging. We also characterized the functions of the genes identified by gene targeting and tagging.

Report

(4 results)
  • 2012 Annual Research Report   Final Research Report ( PDF )
  • 2011 Annual Research Report
  • 2010 Annual Research Report
  • Research Products

    (53 results)

All 2013 2012 2011 2010 Other

All Journal Article (21 results) (of which Peer Reviewed: 21 results) Presentation (28 results) (of which Invited: 1 results) Book (3 results) Remarks (1 results)

  • [Journal Article] Homologous recombination-mediated gene targeting in the liverwort Marchantia polymorpha2013

    • Author(s)
      K. Ishizaki, Y. Johzuka-Hisatomi, S. Ishida, S. Iida and T. Kohchi
    • Journal Title

      L. Sci. Rep

      Volume: 3 Issue: 1 Pages: 1532-1532

    • DOI

      10.1038/srep04532

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] TAWAWA1, a novel regulator of rice inflorescence architecture, functions through the suppression of meristem phase transition.2013

    • Author(s)
      Akiko Yoshida
    • Journal Title

      Proc. Natl. Acad. Sci. USA

      Volume: 110 Issue: 2 Pages: 767-772

    • DOI

      10.1073/pnas.1216151110

    • Related Report
      2012 Annual Research Report 2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Homologous recombination-mediated gene targeting in the liverwort Marchantia polymorpha L.2013

    • Author(s)
      K. Ishizaki
    • Journal Title

      Scientific Reports

      Volume: 3 Issue: 1 Pages: 1532-1532

    • DOI

      10.1038/srep01532

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A null mutation of ROS1a for DNA demethylation in rice is not transmittable to progeny2012

    • Author(s)
      A. Ono, K. Yamaguchi, S. Fukada-Tanaka, R. Terada, T. Mitsui and S. Iida
    • Journal Title

      Plant J

      Volume: 71 Issue: 4 Pages: 564-574

    • DOI

      10.1111/j.1365-313x.2012.05009.x

    • Related Report
      2012 Final Research Report 2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Activation and epigenetic regulation of DNA transposon nDart1 in rice2012

    • Author(s)
      C.-H. Eun, K. Takagi, K.-I. Park, M. Maekawa, S. Iida and K. Tsugane
    • Journal Title

      Plant Cell Physiol

      Volume: 53 Issue: 5 Pages: 857-868

    • DOI

      10.1093/pcp/pcs060

    • NAID

      10030654499

    • Related Report
      2012 Annual Research Report 2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Development of EST-SSR markers of <i>Ipomoea nil</i>2012

    • Author(s)
      T. Ly, H. Fukuoka, A. Otaka, A. Hoshino, S. Iida, E. Nitasaka, N. Watanabe and T. Kuboyama
    • Journal Title

      Breeding Science

      Volume: 62 Issue: 1 Pages: 99-104

    • DOI

      10.1270/jsbbs.62.99

    • NAID

      130004146342

    • ISSN
      1344-7610, 1347-3735
    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] The molecular basis of incomplete dominance at the A locus of CHS-D in the common morning glory, Ipomoea purpurea2011

    • Author(s)
      Y. Johzuka-Hisatomi, H. Noguchi and S. Iida
    • Journal Title

      J. Plant Res

      Volume: 124 Pages: 299-304

    • NAID

      10027868379

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Examination of transpositional activity of nDart1 at different stages of rice development2011

    • Author(s)
      M. Hayashi-Tsugane, M. Maekawa, H. Kobayashi, S. Iida and K. Tsugane
    • Journal Title

      Genes Genet, Syst

      Volume: 86 Pages: 215-219

    • NAID

      10029679006

    • URL

      https://www.jstage.jst.go.jp/article/ggs/86/3/86_3_215/_article

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] A bHLH transcription factor, DvIVS, is involved in regulation of anthocyanin synthesis in dahlia (Dahlia variabilis2011

    • Author(s)
      S. Ohno, M. Hosokawa, A. Hoshino, Y. Kitamura, Y. Morita, K.-I. Park, A.Nakashima, A. Deguchi, F. Tatsuzawa, M. Doi, S, Iida and S. Yazawa
    • Journal Title

      J. Exp. Bot

      Volume: 62 Issue: 14 Pages: 5105-5116

    • DOI

      10.1093/jxb/err216

    • NAID

      120005477211

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Anthocyanin pigmentation controlled by speckled and c-1 mutations of Japanese morning glory2011

    • Author(s)
      N. Saito, F. Tatsuzawa, A. Hoshino, Y. Abe, M. Ichimura, M. Yokoi, K. Toki, Y. Morita, S. Iida and T. Honda
    • Journal Title

      J. Japan Soc. Hort. Sci

      Volume: 80 Pages: 452-460

    • NAID

      130004951646

    • URL

      https://www.jstage.jst.go.jp/article/jjshs1/80/4/80_4_452/_article

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Constitutive expression of the GIGANTEA ortholog affects circadian rhythms and suppresses one-shot induction of flowering in Pharbitis nil, a typical short-day plant2011

    • Author(s)
      Y. Higuchi, K. Sage-Ono, R. Sasaki, N. Ohtsuki, A. Hoshino, S. Iida, H. Kamada and M. Ono
    • Journal Title

      Plant Cell Physiol

      Volume: 52 Issue: 4 Pages: 638-650

    • DOI

      10.1093/pcp/pcr023

    • NAID

      10029710157

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] A rice mutant displaying a heterochronically elongated internode carries a 100 kb deletion2011

    • Author(s)
      M. Hayashi-Tsugane, M. Maekawa, Q. Qian, H. Kobayashi, S. Iida and K. Tsugane
    • Journal Title

      J. Genet. Genomics

      Volume: 38 Pages: 123-128

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] メンデルの法則における不完全優性と植物の遺伝子量効果2011

    • Author(s)
      定塚(久富) 恵世、山内卓樹、飯田滋
    • Journal Title

      生化学

      Volume: 83 Pages: 638-642

    • NAID

      10029322323

    • URL

      http://www.jbsoc.or.jp/event/magazine/pdf/83-07-09.pdf

    • Related Report
      2012 Final Research Report 2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Examination of transpositional activity of nDart1 at different stages of rice development.2011

    • Author(s)
      M. Hayashi-Tsugane
    • Journal Title

      Genes Genet. Syst.

      Volume: 86 Pages: 215-219

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A rice mutant displaying a heterochronically elongated internode carries a 100 kb deletion.2011

    • Author(s)
      M. Hayashi-Tsugane
    • Journal Title

      J. Genet. Genomics

      Volume: 38 Issue: 3 Pages: 123-128

    • DOI

      10.1016/j.jgg.2011.02.004

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A rice mutant displaying a heterochronically elongated internode carries a 100 kb deletion.2011

    • Author(s)
      Hayashi-Tsugane, M.
    • Journal Title

      J.Genet.Genomics

      Volume: (印刷中)

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] メンデルの法則における不完全優性と植物の遺伝子量効果2011

    • Author(s)
      定塚(久富)恵世
    • Journal Title

      生化学

      Volume: (印刷中)

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Transposition and target preferences of an active nonautonomous DNA transposon nDart1 and its relatives belonging to the hAT superfamily in rice2010

    • Author(s)
      K. Takagi, M. Maekawa, K. Tsugane and S. Iida
    • Journal Title

      Mol. Genet. Genomics

      Volume: 284 Issue: 5 Pages: 343-355

    • DOI

      10.1007/s00438-010-0569-9

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Cre-loxP mediated marker elimination and gene reactivation at the waxy locus created in rice genome based on strong positive-negative selection2010

    • Author(s)
      R. Terada, M. Nagahara, K. Furukawa, M. Shimamoto, K. Yamaguchi and S. Iida
    • Journal Title

      Plant Biotechnol

      Volume: 27 Pages: 29-37

    • NAID

      10026411331

    • URL

      http://www.wdc-jp.biz/pdf_store/jspcmb/pdf/pb27_1/27_29.pdf

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Cre-lox mediated marker elimination and gene reactivation at waxy locus created in the rice genome based on strong positive-negative selection.2010

    • Author(s)
      Terada, R.
    • Journal Title

      Plant Biotechnol.

      Volume: 27 Pages: 29-37

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Transposition and target preferences of an active nonautonomous DNA transposon nDart1 and its relatives belonging to the hAT superfamily in rice.2010

    • Author(s)
      Takagi, K.
    • Journal Title

      Mol.Gen.Genomics

      Volume: 284 Pages: 343-355

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Presentation] A transposon suppressor Dart-canceller in the wild rice2013

    • Author(s)
      K. Tsugane, H. Nishimura, Mhayashi-Tsugane, S. Iida and M. Maekawa
    • Organizer
      第54回日本植物生理学会年会
    • Place of Presentation
      岡山
    • Related Report
      2012 Final Research Report
  • [Presentation] Identification of the transposon-tagged gene essential for chloloplast biogenesis in rice2013

    • Author(s)
      M. Hayashi-Tsugane, H. Takahara, N. Ahmed, E. Himi, K. Takagi, S. Iida, M.Maekawa and K. Tsugane
    • Organizer
      第54回日本植物生理学会年会
    • Place of Presentation
      岡山
    • Related Report
      2012 Final Research Report
  • [Presentation] EST-SSR と EST-SNP を用いたアサガオ連鎖地図の作成2013

    • Author(s)
      伊東拓朗、奥野菫、勝山弘章、森山拳斗、仁田坂英二、星野敦、飯田滋、福岡浩之、磯部祥子、佐藤修正、渡部信義、久保山勉
    • Organizer
      日本育種学会第123回講演会
    • Place of Presentation
      東京
    • Related Report
      2012 Final Research Report
  • [Presentation] A transposon suppressor Dart-canceller in the wild rice.2013

    • Author(s)
      K. Tsugane
    • Organizer
      第54回日本植物生理学会年会
    • Place of Presentation
      岡山
    • Related Report
      2012 Annual Research Report
  • [Presentation] イネ内在性 DNA トランスポゾン nDart1 のエピジェネティックな転移活性の制御2012

    • Author(s)
      栂根一夫、Eun Chang-Ho、高木恭子、栂根美佳、飯田滋
    • Organizer
      日本育種学会第122回講演会
    • Place of Presentation
      京都
    • Related Report
      2012 Final Research Report
  • [Presentation] コシヒカリ nDart1-0 タグラインの育成2012

    • Author(s)
      西村秀希、氷見英子、 飯田滋、栂根一夫、前川雅彦
    • Organizer
      日本育種学会第122回講演会
    • Place of Presentation
      京都
    • Related Report
      2012 Final Research Report
  • [Presentation] 野生イネゲノム中の栽培イネトランスポゾンを抑制する新規因子2012

    • Author(s)
      栂根一夫、栂根美佳、 飯田滋、前川雅彦
    • Organizer
      日本遺伝学会第84回大会
    • Place of Presentation
      福岡
    • Related Report
      2012 Final Research Report
  • [Presentation] アサガオの模様にみるエピジェネティックな遺伝子発現制御の獲得機構2012

    • Author(s)
      星野敦、朴慶一、飯田滋
    • Organizer
      日本進化学会第14回東京大会
    • Place of Presentation
      東京
    • Related Report
      2012 Final Research Report
  • [Presentation] Epigenetic regulation of flower variegation in Ipomoea tricolor2012

    • Author(s)
      A. Hoshino, K.-I. Park and S. Iida
    • Organizer
      第8回生物学国際高等コンファレンス(OBC8)
    • Place of Presentation
      岡崎
    • Related Report
      2012 Final Research Report
  • [Presentation] アサガオの模様を生み出す small RNA の機能と制御2012

    • Author(s)
      森田裕将、星野敦、飯田滋
    • Organizer
      第53回日本植物生理学会年会
    • Place of Presentation
      京都
    • Related Report
      2012 Final Research Report
  • [Presentation] イネ内在性DNAトランスポゾンnDartのエピゲネティックな転移活性の制御2012

    • Author(s)
      栂根一夫
    • Organizer
      日本育種学会第122回講演会
    • Place of Presentation
      京都
    • Related Report
      2012 Annual Research Report
  • [Presentation] コシヒカリnDart1-0タグラインの育成2012

    • Author(s)
      西村秀希
    • Organizer
      日本育種学会第122回講演会
    • Place of Presentation
      京都
    • Related Report
      2012 Annual Research Report
  • [Presentation] 野生イネゲノム中の栽培イネトランスポゾを抑制する新規因子2012

    • Author(s)
      栂根一夫
    • Organizer
      日本遺伝学会第84回大会
    • Place of Presentation
      福岡
    • Related Report
      2012 Annual Research Report
  • [Presentation] DNA トランスポゾン nDart1 挿入により生じたイネ半優性矮化多分げつ変異体の解析2011

    • Author(s)
      栂根(林)美佳、前川雅彦、 飯田滋、栂根一夫
    • Organizer
      第34回日本分子生物学会年会
    • Place of Presentation
      横浜
    • Related Report
      2012 Final Research Report
  • [Presentation] イオンビーム処理によるイネのDNA トランスポゾン Dart の活性化2011

    • Author(s)
      前川雅彦、西村秀希、氷見英子、 飯田滋、栂根一夫
    • Organizer
      日本育種学会第122回講演会
    • Place of Presentation
      福井
    • Related Report
      2012 Final Research Report
  • [Presentation] アザシチジン処理で活性化した自律性因子 Dart の特定2011

    • Author(s)
      西村秀希、氷見英子、 飯田滋、栂根一夫、前川雅彦
    • Organizer
      第122回日本育種学会講演会
    • Place of Presentation
      福井
    • Related Report
      2012 Final Research Report
  • [Presentation] アサガオの模様から RNA サイレンシングの制御と機能を探る2011

    • Author(s)
      森田裕将、星野敦、飯田滋
    • Organizer
      日本遺伝学会第83回大会
    • Place of Presentation
      京都
    • Related Report
      2012 Final Research Report
  • [Presentation] ソライロアサガオの模様に係わるエピジェネティクス2011

    • Author(s)
      星野敦、朴慶一、崔丁斗、飯田滋
    • Organizer
      第52回日本植物生理学会年会
    • Place of Presentation
      仙台
    • Related Report
      2012 Final Research Report
  • [Presentation] イネ DNA トランスポゾン nDart1 の挿入領域の特徴と利用2011

    • Author(s)
      栂根一夫、高木恭子、栂根(林)美佳、前川雅彦、飯田滋
    • Organizer
      日本育種学会第121回講演会
    • Place of Presentation
      横浜
    • Related Report
      2012 Final Research Report
  • [Presentation] Modification of an endogenous rice gene by homologous recombination and its applications.2011

    • Author(s)
      Iida, S.
    • Organizer
      GCOE国際拠点形成シンポジウム
    • Place of Presentation
      静岡
    • Related Report
      2011 Annual Research Report
    • Invited
  • [Presentation] Rice functional genomics: Homologous recombination-promoted gene targeting of endogenous genes.2011

    • Author(s)
      Iida, S.
    • Organizer
      The 4th International Conference on Health and Longevity Science
    • Place of Presentation
      静岡
    • Related Report
      2011 Annual Research Report
  • [Presentation] アザシチジン処理で活性化した自律性因子Dartの特定2011

    • Author(s)
      西村秀希
    • Organizer
      日本育種学会 第120回講演会
    • Place of Presentation
      福井
    • Related Report
      2011 Annual Research Report
  • [Presentation] イオンビーム処理によるイネのDNAトランスポゾンDartの活性化2011

    • Author(s)
      前川雅彦
    • Organizer
      日本育種学会第122回講演会
    • Place of Presentation
      福井
    • Related Report
      2011 Annual Research Report
  • [Presentation] Functional analysis of Os ROS1a by homologous recombination-promoted gene targeting.2010

    • Author(s)
      Ono, A.
    • Organizer
      第33回日本分子生物学会年会及び第83回日本生化学会大会合同大会
    • Place of Presentation
      神戸
    • Year and Date
      2010-12-04
    • Related Report
      2010 Annual Research Report
  • [Presentation] Functional analysis of OsROS1aby homologous recombination-promoted gene targeting2010

    • Author(s)
      A. Ono, K. Yamaguchi, Y. JohtsukaHisatomi, T. Mitsui, R. Terada and S. Iida
    • Organizer
      第33回日本分子生物学会年会及び第83回日本生化学会大会合同大会
    • Place of Presentation
      神戸
    • Related Report
      2012 Final Research Report
  • [Presentation] 大規模欠失によるイネ節間伸張異常変異体の遺伝学的解析2010

    • Author(s)
      栂根美佳、前川雅彦、Qian Qian、小林裕和、飯田滋、栂根一夫
    • Organizer
      第33回日本分子生物学会年会及び第83回日本生化学会大会合同大会
    • Place of Presentation
      神戸
    • Related Report
      2012 Final Research Report
  • [Presentation] mRNA の網羅的塩基配列解読によるアサガオ SNP マーカーの作出2010

    • Author(s)
      リートン、福岡浩之、星野敦、飯田滋、仁田坂英二、久保山勉
    • Organizer
      日本育種学会第118回講演会
    • Place of Presentation
      秋田
    • Related Report
      2012 Final Research Report
  • [Presentation] イネ内在性 DNA トランスポゾン nDart の挿入部位指向性と遺伝子タギングへの応用2010

    • Author(s)
      栂根一夫、高木恭子、前川雅彦、 飯田滋
    • Organizer
      イネ遺伝学・分子生物学ワークショップ2010
    • Place of Presentation
      つくば
    • Related Report
      2012 Final Research Report
  • [Book] Effective contribution of the nDart transposon-tagging system to rice functional genomics. K. V. Urbano ed2011

    • Author(s)
      M. Maekawa, K. Tsugane and S. Iida
    • Publisher
      Advances in Genetics Research rice functional genomics. K. V. Urbano ed
    • Related Report
      2012 Final Research Report
  • [Book] Adv. Genetics Res. Vol. 42011

    • Author(s)
      Maekawa, M.
    • Publisher
      Nova Sci. Pub. Inc.
    • Related Report
      2011 Annual Research Report
  • [Book] Adv.Genetics Res.Vol.42011

    • Author(s)
      Maekawa, M.
    • Publisher
      Nova Sci.Pub.Inc.(印刷中)
    • Related Report
      2010 Annual Research Report
  • [Remarks]

    • URL

      http://sfns.u-shizuoka-ken.ac.jp/molgen/

    • Related Report
      2010 Annual Research Report

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Published: 2010-08-23   Modified: 2019-07-29  

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