| Project/Area Number |
22370001
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Genetics/Genome dynamics
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| Research Institution | University of Shizuoka |
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Principal Investigator |
IIDA Shigeru 静岡県立大学, 大学院・食品栄養環境科学研究院, 客員教授 (30012777)
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| Co-Investigator(Kenkyū-buntansha) |
JOHZUKA Yasuyo (HISATOMI Yasuyo) 静岡県立大学, 大学院・薬学研究科, 博士研究員 (60517887)
KINOSHITA Tetsu 奈良先端科学技術大学院大学, バイオサイエンス研究科, 研究チーム長 (60342630)
ISHIZAKI Kimitsune 京都大学, 大学院・生命科学研究科, 助教 (00452293)
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| Co-Investigator(Renkei-kenkyūsha) |
KOBAYASHI Hirokazu 静岡県立大学, 大学院・食品栄養環境科学研究院, 教授 (80170348)
NOGUCHI Hiroshi 静岡県立大学, 薬学部, 教授 (60126141)
WATANABE Kenji 静岡県立大学, 薬学部, 准教授 (50360938)
JOHZUKA Yasuyo (HISATOMI Yasuyo) 静岡県立大学, 大学院・食品栄養環境科学研究院, 客員研究員 (60517887)
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| Project Period (FY) |
2010 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥18,850,000 (Direct Cost: ¥14,500,000、Indirect Cost: ¥4,350,000)
Fiscal Year 2012: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2011: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2010: ¥10,010,000 (Direct Cost: ¥7,700,000、Indirect Cost: ¥2,310,000)
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| Keywords | 植物 / 分子遺伝 / 逆遺伝学 / 遺伝子 / 突然変異 / バイオテクノロジー / ゲノム / 遺伝学 / 組換え機構 |
|---|
| Research Abstract |
To facilitate reverse genetic approaches, which include homologous recombination-mediated gene targeting using positive-negative selection and gene tagging employing endogenous nDart1-related DNA transposons, we were attempting to elucidate recombination mechanisms and their regulation mechanisms associated with gene targeting and tagging. We also characterized the functions of the genes identified by gene targeting and tagging.
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