Evolution of the function maintenance system of plant organelle genome by C-to-U RNA editing
Project/Area Number |
22370002
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Research Category |
Grant-in-Aid for Scientific Research (B)
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Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Genetics/Genome dynamics
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Research Institution | Kyoto Prefectural University |
Principal Investigator |
OBOKATA Junichi 京都府立大学, 大学院・生命環境科学研究科, 教授 (50185667)
|
Co-Investigator(Kenkyū-buntansha) |
SHIINA Takashi 京都府立大学, 生命環境科学研究科, 教授 (10206039)
SUGIYAMA Yasuo 名古屋大学, 遺伝子実験施設, 准教授 (70154507)
|
Project Period (FY) |
2010 – 2012
|
Project Status |
Completed (Fiscal Year 2012)
|
Budget Amount *help |
¥18,720,000 (Direct Cost: ¥14,400,000、Indirect Cost: ¥4,320,000)
Fiscal Year 2012: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2011: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2010: ¥7,800,000 (Direct Cost: ¥6,000,000、Indirect Cost: ¥1,800,000)
|
Keywords | 植物オルガネラゲノム / 葉緑体RNA編集 / PPRタンパク質 / RNA分解酵素 / C-to-U型RNA編集 / 葉緑体ゲノム / 植物ゲノム / RNA編集因子 / シロイヌナズナ / psbE / 単離精製 / RNA-タンパク質複合体 |
Research Abstract |
Catalytic molecules of the plant organellar RNA editing has long been unidentified. We attempted to isolate and characterize those molecules, using the psbE gene of Arabidopsis thaliana as a model. As a result, we succeeded in concentrating the catalytic activity from the chloroplast lysates by biochemical means, and found that RNase-sensitive component(s) should be involved in the catalytic reaction. Based on this we propose a tentative model of the C-to-U editing machinery.
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Report
(4 results)
Research Products
(20 results)
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[Journal Article] Pentatricopeptide repeat proteins involved in plant organellar RNA editing2013
Author(s)
Yagi,Y., Tachikawa, M., Noguchi, H., Satoh, S., Obokata, Jand Nakamura, T
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Journal Title
RNA Biology
Volume: 10
Pages: 1-7
Related Report
Peer Reviewed
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[Journal Article] efense response of a pepper cultivar cv.Sy-2 is induced at temperatures below 24℃2011
Author(s)
Koeda, S., Hosokawa, M., Kang, B.C., Tanaka, C., Choi, D., Sano, S., Shiina, T., Doi, M., Yazawa
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Journal Title
J Plant Res
Volume: (印刷中)
Related Report
Peer Reviewed
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[Journal Article] Nuclear/Nucleolar GTPase 2 Proteins as a Subfamily of YlqF/YawG GTPases Function in Pre-60S Ribosomal Subunit Maturation of Mono- and Dicotyledonous Plants.2010
Author(s)
Im, C.H., Hwang, S.M., Son, Y.S., Heo, J.B., Bang, W.Y., Suwastika, I.N., Shiina, T., Bahk, J.D
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Journal Title
J Biol Chem.
Volume: 286
Pages: 8620-8632
Related Report
Peer Reviewed
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