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Evolution of the function maintenance system of plant organelle genome by C-to-U RNA editing

Research Project

Project/Area Number 22370002
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Genetics/Genome dynamics
Research InstitutionKyoto Prefectural University

Principal Investigator

OBOKATA Junichi  京都府立大学, 大学院・生命環境科学研究科, 教授 (50185667)

Co-Investigator(Kenkyū-buntansha) SHIINA Takashi  京都府立大学, 生命環境科学研究科, 教授 (10206039)
SUGIYAMA Yasuo  名古屋大学, 遺伝子実験施設, 准教授 (70154507)
Project Period (FY) 2010 – 2012
Project Status Completed (Fiscal Year 2012)
Budget Amount *help
¥18,720,000 (Direct Cost: ¥14,400,000、Indirect Cost: ¥4,320,000)
Fiscal Year 2012: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2011: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2010: ¥7,800,000 (Direct Cost: ¥6,000,000、Indirect Cost: ¥1,800,000)
Keywords植物オルガネラゲノム / 葉緑体RNA編集 / PPRタンパク質 / RNA分解酵素 / C-to-U型RNA編集 / 葉緑体ゲノム / 植物ゲノム / RNA編集因子 / シロイヌナズナ / psbE / 単離精製 / RNA-タンパク質複合体
Research Abstract

Catalytic molecules of the plant organellar RNA editing has long been unidentified. We attempted to isolate and characterize those molecules, using the psbE gene of Arabidopsis thaliana as a model. As a result, we succeeded in concentrating the catalytic activity from the chloroplast lysates by biochemical means, and found that RNase-sensitive component(s) should be involved in the catalytic reaction. Based on this we propose a tentative model of the C-to-U editing machinery.

Report

(4 results)
  • 2012 Annual Research Report   Final Research Report ( PDF )
  • 2011 Annual Research Report
  • 2010 Annual Research Report
  • Research Products

    (20 results)

All 2013 2012 2011 2010 Other

All Journal Article (7 results) (of which Peer Reviewed: 7 results) Presentation (11 results) Remarks (2 results)

  • [Journal Article] Pentatricopeptide repeat proteins involved in plant organellar RNA editing2013

    • Author(s)
      Yagi,Y., Tachikawa, M., Noguchi, H., Satoh, S., Obokata, Jand Nakamura, T
    • Journal Title

      RNA Biology

      Volume: 10 Pages: 1-7

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Pentatricopeptide repeat proteins involved in plant organellar RNA editing2013

    • Author(s)
      Yusuke Yagi
    • Journal Title

      RNA Biology

      Volume: 未定

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Characteristics of core promoter types with respect to gene structure and expression in Arabidopsis thaliana2012

    • Author(s)
      Yamamoto YY., Yoshioka Y., Hyakumachi M., Obokata J.
    • Journal Title

      DNA Research

      Volume: 18 Issue: 5 Pages: 3634-35636

    • DOI

      10.1093/dnares/dsr020

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Transcriptome analysis of respiration-responsive genes in Chlamydomonas reinhardtii : Mitochondrial retrograde signaling coordinates the genes for cell proliferation with energy-producing metabolisms.2011

    • Author(s)
      Matsuo, M., Hachisu, R., Tabata, S., Fukuzawa, H., Obokata, J.
    • Journal Title

      Plant Cell Physiol

      Volume: 52 Pages: 333-343

    • NAID

      10029708866

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] efense response of a pepper cultivar cv.Sy-2 is induced at temperatures below 24℃2011

    • Author(s)
      Koeda, S., Hosokawa, M., Kang, B.C., Tanaka, C., Choi, D., Sano, S., Shiina, T., Doi, M., Yazawa
    • Journal Title

      J Plant Res

      Volume: (印刷中)

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Selective Excitation of Photosystems in Chloroplasts inside Plant Leaves Observed by Near-Infrared Laser-Based Fluorescence Spectral Microscopy.2010

    • Author(s)
      Hasegawa, M., Shiina, T., Terazima, M., Kumazaki, S.
    • Journal Title

      Plant Cell Physiol

      Volume: 51 Pages: 225-238

    • NAID

      10027346696

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Nuclear/Nucleolar GTPase 2 Proteins as a Subfamily of YlqF/YawG GTPases Function in Pre-60S Ribosomal Subunit Maturation of Mono- and Dicotyledonous Plants.2010

    • Author(s)
      Im, C.H., Hwang, S.M., Son, Y.S., Heo, J.B., Bang, W.Y., Suwastika, I.N., Shiina, T., Bahk, J.D
    • Journal Title

      J Biol Chem.

      Volume: 286 Pages: 8620-8632

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Presentation] 葉緑体のC-to-U RNA 編集活性はRNase によって失活する2013

    • Author(s)
      立川誠、長谷川綾香、高林厚史、種村尚典、溝田香織、諏佐瑞穂、野村明子、若杉達也、小保方潤一
    • Organizer
      第15回日本RNA学会年会
    • Place of Presentation
      松山
    • Related Report
      2012 Final Research Report
  • [Presentation] 遺伝子重複型偽遺伝子の転写状態の維持におけるATG配列の役割2013

    • Author(s)
      佐藤壮一郎
    • Organizer
      第54回日本植物生理学会年会
    • Place of Presentation
      岡山
    • Related Report
      2012 Annual Research Report
  • [Presentation] 高等植物において遺伝子コード領域の存在は転写型シーンサイレンジンクを抑制する2012

    • Author(s)
      松尾充啓、佐藤壮一郎、工藤久幸、安井孝彰、木村宏、中邨真之、山本義治、小保方潤一
    • Organizer
      第53回日本植物生理学会年会
    • Place of Presentation
      京都
    • Year and Date
      2012-03-17
    • Related Report
      2011 Annual Research Report
  • [Presentation] 遺伝子重複領域内のATG開始コドンが転写状態に与える影響について2012

    • Author(s)
      佐藤壮一郎、松尾充啓、工藤久幸、安井孝彰、木村宏、中邨真之、山本義治、小保方潤一
    • Organizer
      第53回日本植物生理学会年会
    • Place of Presentation
      京都
    • Year and Date
      2012-03-17
    • Related Report
      2011 Annual Research Report
  • [Presentation] A new driving force for the functional gene transfer during endosymbiotic evolution: pol II promoter de novo origination2012

    • Author(s)
      Junichi Obokata
    • Organizer
      Protists 2012
    • Place of Presentation
      オスロ
    • Related Report
      2012 Annual Research Report
  • [Presentation] シロイヌナズナTFIIF変異体のゲノムワイド転写開始点解析2012

    • Author(s)
      佐藤壮一郎
    • Organizer
      第35回日本分子生物学会年会
    • Place of Presentation
      福岡
    • Related Report
      2012 Annual Research Report
  • [Presentation] 遺伝子重複領域内のATG開始コドンの保存性が転写状態の持続性に及ぼす影響について所2011

    • Author(s)
      佐藤壮一郎、松尾充啓、工藤久幸、安井孝彰、木村宏、中邨真之、山本義治、小保方潤一
    • Organizer
      第34回日本分子生物学会
    • Place of Presentation
      横浜
    • Year and Date
      2011-12-15
    • Related Report
      2011 Annual Research Report
  • [Presentation] 植物の核ゲノムにはATG開始コドンを介したプロモーター機能領域の出現メカニズムがある2011

    • Author(s)
      松尾充啓、佐藤壮一郎、工藤久幸、安井孝彰、木村宏、中邨真之、山本義治、小保方潤一
    • Organizer
      日本遺伝学会第83回大会
    • Place of Presentation
      京都
    • Year and Date
      2011-09-20
    • Related Report
      2011 Annual Research Report
  • [Presentation] 植物ゲノム上でのプロモーター機能領域の出現に対するATG開始コドンの役割2011

    • Author(s)
      佐藤壮一郎、松尾充啓、工藤久幸、安井孝彰、木村宏、中邨真之、山本義治、小保方潤一
    • Organizer
      日本植物学会第75回大会
    • Place of Presentation
      東京
    • Year and Date
      2011-09-17
    • Related Report
      2011 Annual Research Report
  • [Presentation] 一次共生者の遺伝子群はどのように宿主核の発現系に取り込まれるのか2010

    • Author(s)
      小保方潤一
    • Organizer
      第33回日本分子生物学会年会
    • Place of Presentation
      神戸ポートアイランド国際会議場
    • Year and Date
      2010-12-09
    • Related Report
      2010 Annual Research Report
  • [Presentation] How the chloroplast-nucelar genome system has evolved in plants2010

    • Author(s)
      Obokata, J.
    • Organizer
      ISBDS2010 International Symposium on Biodiversity Sciences "Genome, Evolution and Environment"
    • Place of Presentation
      ホテル・ルブラ王山名古屋
    • Year and Date
      2010-08-02
    • Related Report
      2010 Annual Research Report
  • [Remarks]

    • URL

      http://www2.kpu.ac.jp/life_environ/plant_genome_bio/

    • Related Report
      2012 Final Research Report
  • [Remarks] 京都府立大学植物ゲノム情報学研究室

    • URL

      http://www2.kpu.ac.jp/life_environ/plant_genome_bio/Site/Top.html

    • Related Report
      2012 Annual Research Report

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Published: 2010-08-23   Modified: 2019-07-29  

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