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Eco-omics analyse of microbial sulfur cycle in aquatic environments of cryosphere

Research Project

Project/Area Number 22370005
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Ecology/Environment
Research InstitutionHokkaido University

Principal Investigator

FUKUI Manabu  北海道大学, 低温科学研究所, 教授 (60305414)

Co-Investigator(Kenkyū-buntansha) KOJIMA Hisaya  北海道大学, 低温科学研究所, 助教 (70400009)
Project Period (FY) 2010-04-01 – 2014-03-31
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥18,460,000 (Direct Cost: ¥14,200,000、Indirect Cost: ¥4,260,000)
Fiscal Year 2013: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Fiscal Year 2012: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2011: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2010: ¥7,020,000 (Direct Cost: ¥5,400,000、Indirect Cost: ¥1,620,000)
Keywords硫黄循環 / メタゲノム / プロテオミクス / 硫酸還元 / 湖沼 / 硫黄酸化 / 機能遺伝子 / 群集構造 / ゲノミクス / 微生物 / 群集 / 代謝
Research Abstract

Microbial sulfur cycle in aquatic systems of cryosphere is still unclear although the significance of systems linking carbon and nitrogen cycles has been pointed out. Analysis of aprA gene, which encodes a key enzyme in dissimilatory sulfate reduction and sulfur oxidation, showed that newly isolated Sulfuricella denitrificans-like sulfur oxidizer is a key species from geographically distant lakes in Antarctica and Japan. The complete genome sequence analysis indicated their unique sulfur metabolic pathway adapting freshwater environment. The proteomics results suggest that maintenance of proper translation is critical for growth under low-temperature.

Report

(5 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Annual Research Report
  • 2011 Annual Research Report
  • 2010 Annual Research Report
  • Research Products

    (45 results)

All 2014 2013 2012 2011 2010 Other

All Journal Article (22 results) (of which Peer Reviewed: 22 results) Presentation (22 results) (of which Invited: 1 results) Remarks (1 results)

  • [Journal Article] Identification of major planktonic sulfur oxidizers in stratified freshwater lake2014

    • Author(s)
      Hisaya Kojima, Tomohiro Watanabe, Tomoya Iwata and Manabu Fukui
    • Journal Title

      PLoS ONE

      Volume: e93877 Issue: 4 Pages: e93877-e93877

    • DOI

      10.1371/journal.pone.0093877

    • NAID

      120005438262

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Sulfurisoma sediminicola gen. nov., sp. nov., a novel facultative autotroph isolated from a freshwater lake2014

    • Author(s)
      Hisaya Kojima and Manabu Fukui
    • Journal Title

      International Journal of Systematic and Evolutionary Microbiology

      Volume: in press Issue: Pt_5 Pages: 1587-1592

    • DOI

      10.1099/ijs.0.057281-0

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Diversity of sulfur-cycle prokaryotes in freshwater lake sediments investigated using aprA as functional marker gene2013

    • Author(s)
      Tomohiro Watanabe, Hisaya Kojima, Yoshinori Takano and Manabu Fukui
    • Journal Title

      Systematic and Applied Microbiology

      Volume: 36 Issue: Pt_10 Pages: 436-443

    • DOI

      10.1099/ijs.0.051854-0

    • Related Report
      2013 Annual Research Report 2013 Final Research Report 2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Effects of temperature on successive anaerobic decomposition of high-molecular organic matter under sulfate-reducing conditions. Estuarine2013

    • Author(s)
      Takato Matsui, Hisaya Kojima, and Manabu Fukui
    • Journal Title

      Coastal and Shelf Science

      Volume: 119 Pages: 139-144

    • DOI

      10.1016/j.ecss.2013.01.003

    • Related Report
      2013 Final Research Report 2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Desulfatitalea tepidiphila gen. nov., sp. nov., a novel sulfate-reducing bacterium isolated from tidal flat sediment2013

    • Author(s)
      Yuriko Higashioka, Hisaya Kojima, Miho Watanabe and Manabu Fukui
    • Journal Title

      International Journal of Systematic and Evolutionary Microbiology

      Volume: 63 Issue: Pt_2 Pages: 761-765

    • DOI

      10.1099/ijs.0.043356-0

    • Related Report
      2013 Final Research Report 2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Sulphate and chloride aerosols during Holocene and last glacial periods preserved in the Talos Dome Ice Core, a peripheral region of Antarctica2013

    • Author(s)
      Yoshinori Iizuka, Barbara Delmonte, Ikumi Oyabu, Torbjorn Karlin, Valter Maggi, Samuel Albani, Manabu Fukui, Takeo Hondoh and Margareta Hansson
    • Journal Title

      Tellus B

      Volume: 65 Issue: 1 Pages: 20197-20197

    • DOI

      10.3402/tellusb.v65i0.20197

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Exploring slime mould diversity in high altitude forests and grasslands by environmental RNA analysis2013

    • Author(s)
      Kamono,A.
    • Journal Title

      FEMS Microbiology Ecology

      Volume: 84 Issue: 1 Pages: 98-108

    • DOI

      10.1111/1574-6941.12042

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Draft genome sequence of a psychrotolerant sulfur-oxidizing bacterium Sulfuricella denitrificans skB26 and proteomic insights into cold adaptation2012

    • Author(s)
      Tomohiro Watanabe, Hisaya Kojima, and Manabu Fukui
    • Journal Title

      Applied and Environmental Microbiology

      Volume: 78 Issue: 18 Pages: 6545-6549

    • DOI

      10.1128/aem.01349-12

    • NAID

      120005228243

    • Related Report
      2013 Final Research Report 2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Filamentous sulfur-oxidizing bacteria of the genus Thioploca from Lake Tonle Sap in Cambodia2012

    • Author(s)
      Fumiko Nemoto, Hisaya Kojima, Akifumi Ohtaka and Manabu Fukui
    • Journal Title

      Aquatic Microbial Ecology

      Volume: 66 Issue: 3 Pages: 295-300

    • DOI

      10.3354/ame01578

    • Related Report
      2013 Final Research Report 2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Holocene lake development and glacial-isostatic uplift at Lake Skallen and Lake Oyako, Lu-tzow-Holm Bay, East Antarctica : based on biogeocheaical facies and molecular signatures.2012

    • Author(s)
      Takano Y, Tyler JJ, Kojima H, Yokoyama Y, Tanabe Y, Sato T, Ogawa ON, Ohkouchi N, Fukui M
    • Journal Title

      Applied Geochemistry

      Volume: 27 Issue: 12 Pages: 2546-2559

    • DOI

      10.1016/j.apgeochem.2012.08.009

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 18S rDNA phylogeny of Lanmproderma and allied genera (Stemonitales, Myxomycetes, Amoebozoa)2012

    • Author(s)
      Fiore-Donno,A.
    • Journal Title

      PLoS ONE

      Volume: 7 Issue: 4 Pages: e35359-e35359

    • DOI

      10.1371/journal.pone.0035359

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Diversity of freshwater Thioploca species and their specific association with filamentous bacterla of the phylum Chloroflexi2011

    • Author(s)
      Fumiko Nemoto, Hisaya Kojima, Manabu Fukui
    • Journal Title

      Environmental Microbiology

      Volume: 62 Issue: 4 Pages: 753-764

    • DOI

      10.1007/s00248-011-9916-6

    • Related Report
      2013 Final Research Report 2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Temperature-dependent differences in community structure of bacteria involved in degradation of petroleum hydrocarbons under sulfate-reducing conditions2011

    • Author(s)
      Yuriko Higashioka, Hisaya Kojima and Manabu Fukui
    • Journal Title

      Journal of Applied and Microbiology

      Volume: 110 Issue: 1 Pages: 314-322

    • DOI

      10.1111/j.1365-2672.2010.04886.x

    • Related Report
      2013 Final Research Report 2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Sulfuritalea hydrogenivorans gen.nov., sp.nov., a novel facultative autotroph isolated from a freshwater lake2011

    • Author(s)
      Kojima, Fukui
    • Journal Title

      International Journal of Systematic and Evolutionary-Microbiology

      Volume: 61 Pages: 1651-1655

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Sulfuricella denitrificans gen. nov., sp. nov., a novel sulfur-oxidizing autotroph isolated from a freshwater lake2010

    • Author(s)
      Hisaya Kojima, and Manabu Fukui
    • Journal Title

      International Journal of Systematic and Evolutionary Microbiology

      Volume: 60 Issue: 12 Pages: 2862-2866

    • DOI

      10.1099/ijs.0.016980-0

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Seasonal changes in organic matter mineralization in a sublittoral sediment and temperature-driven decoupling of key processes2010

    • Author(s)
      Tabuchi, Kojima, Fukui
    • Journal Title

      Microbial Ecology

      Volume: 60 Pages: 551-560

    • NAID

      120003354954

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Sulfuricella denitrificans gen. nov., sp. nov., a novel sulfur-oxidizing autotroph isolated from a freshwater lake2010

    • Author(s)
      Kojima, Fukui
    • Journal Title

      International Journal of Systematic and Evolutionary Microbiology

      Volume: 52 Pages: 2862-2866

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Spatiotemporal variations in an assemblage of closely related planktonicaerobic methanotrophs2010

    • Author(s)
      Tsutsumi, Iwata, Kojima, Fukui
    • Journal Title

      Freahwater Biology

      Volume: 56 Pages: 342-351

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Microbial community structure, pigment composition, and nitrogen source of red snow in Antarctica2010

    • Author(s)
      Fujii, et al.
    • Journal Title

      Microbial Ecology

      Volume: 59 Pages: 466-475

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Temperature-dependent differences in community structure involved in degradation of petroleum hydrocarbons under sulfate-reducing conditions2010

    • Author(s)
      Higashioka, Kojima, Fukui
    • Journal Title

      Journal of Applied Microbiology

      Volume: 100 Pages: 314-322

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Invalidation of Hyperamoeba by Transferring its Species to Other Genera of Myxogastria2010

    • Author(s)
      Fiore-Donno, et al.
    • Journal Title

      International Journal of Syatematic and Evolutionary Microbiology

      Volume: 60 Pages: 2862-2866

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Vertical distribution of major sulfate-reducing bacteria in a eutrophic shallow meromictic lake

    • Author(s)
      Kyoko Kubo, Hisaya Kojima, and Manabu Fukui
    • Journal Title

      Systematic and Applied Microbiology

      Volume: (in press)

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Presentation] 淡水湖の水塊で優占する硫黄酸化細菌の比較ゲノム解析2014

    • Author(s)
      渡邉友浩、小島久弥、福井 学
    • Organizer
      第8回日本ゲノム微生物学会
    • Place of Presentation
      東京農業大学(東京都世田谷区)
    • Related Report
      2013 Annual Research Report
  • [Presentation] 淡水湖沼の硫黄酸化細菌群集における主要構成種の推定2014

    • Author(s)
      渡邉友浩、小島久弥、福井 学
    • Organizer
      第61回日本生態学会
    • Place of Presentation
      広島国際会議場(広島県広島市)
    • Related Report
      2013 Annual Research Report
  • [Presentation] 水界の硫黄循環システムと微生物群集2013

    • Author(s)
      福井 学
    • Organizer
      日本進化学会第15回大会
    • Place of Presentation
      茨城県つくば市(筑波大 学)
    • Year and Date
      2013-08-30
    • Related Report
      2013 Final Research Report
  • [Presentation] Comparative genomic analysis of two betaproteobacterial sulfur oxidizers, Sulfuricella denitrificans skB26 and Sulfuritalea hydrogenivorans sk43H, isolated from the same freshwater lake2013

    • Author(s)
      Watanabe, T., Kojima, H. and Fukui, M
    • Organizer
      第5回ヨーロッパ微生物学連合大会
    • Place of Presentation
      ライプチヒ国際会議場(Leipzig, Germany)
    • Related Report
      2013 Annual Research Report
  • [Presentation] Microbial community structure of a shallow meromictic lake, Lake Harutori, Japan2013

    • Author(s)
      Kubo, K., Kojima, H. and Fukui,M
    • Organizer
      第5回ヨーロッパ微生物学連合大会
    • Place of Presentation
      ライプチヒ国際会議場(Leipzig, Germany)
    • Related Report
      2013 Annual Research Report
  • [Presentation] Desulfotomaculum sp. SR45T, a novel sulfate reducer possessing multiple copies of 16S rRNA gene with sequence variation2013

    • Author(s)
      Watanabe, M., Kojima, H. and Fukui,M.
    • Organizer
      第5回ヨーロッパ微生物学連合大会
    • Place of Presentation
      ライプチヒ国際会議場(Leipzig, Germany9
    • Related Report
      2013 Annual Research Report
  • [Presentation] 水界の硫黄循環システムと微生物群集2013

    • Author(s)
      福井 学, 小島久弥
    • Organizer
      第15回進化学会
    • Place of Presentation
      筑波大学(茨城県つくば市)
    • Related Report
      2013 Annual Research Report
  • [Presentation] 淡水湖沼の硫黄酸化細菌群集における主要構成種の特定とゲノム解析2013

    • Author(s)
      渡邉友浩、小島久弥、福井 学
    • Organizer
      第29回日本微生物生態学会
    • Place of Presentation
      鹿児島大学(鹿児島県鹿児島市)
    • Related Report
      2013 Annual Research Report
  • [Presentation] Characteristics of Alicyclobacillaceae bacterium strain skLN1, a novel thermophilic bacterium isolated from freshwater lake sediment2013

    • Author(s)
      Watanabe, M., Kojima, H. and Fukui,M.
    • Organizer
      第29回日本微生物生態学会
    • Place of Presentation
      鹿児島大学(鹿児島県鹿児島市)
    • Related Report
      2013 Annual Research Report
  • [Presentation] 汽水性部分循環湖におけるメタン酸化細菌の群集構造解析2013

    • Author(s)
      小暮耕平、小島久弥、福井 学
    • Organizer
      第29回日本微生物生態学会
    • Place of Presentation
      鹿児島大学(鹿児島県鹿児島市)
    • Related Report
      2013 Annual Research Report
  • [Presentation] 脱窒とカップリングした嫌気 的メタン酸化2012

    • Author(s)
      福井 学
    • Organizer
      日本微生物生態学会第28回大会
    • Place of Presentation
      愛知県豊橋市(豊橋科学技術大学)
    • Year and Date
      2012-09-21
    • Related Report
      2013 Final Research Report
  • [Presentation] 水界の硫黄循環システムと微生物群集2012

    • Author(s)
      福井 学
    • Organizer
      地球惑星科学合同大会2012年度大会
    • Place of Presentation
      千葉県船橋市(幕張メッセ)
    • Year and Date
      2012-05-24
    • Related Report
      2013 Final Research Report
  • [Presentation] Characterization of a novel spore-forming sulfate reducer belonging to the Desulfotomaculum subcluster Ie2012

    • Author(s)
      Watanabe, M.
    • Organizer
      第28回日本微生物生態学会
    • Place of Presentation
      豊橋技術科学大学(豊橋市)
    • Related Report
      2012 Annual Research Report
  • [Presentation] 脱窒とカップルした嫌気的メタン酸化:新規の電子の流れ2012

    • Author(s)
      福井学
    • Organizer
      第28回日本微生物生態学会
    • Place of Presentation
      豊橋技術科学大学(豊橋市)
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Presentation] Characterization of a novel sulfate-reducing bacterium, as the second isolated member of the Desulfotomaculum subcluster Ie2012

    • Author(s)
      Watanabe, M.
    • Organizer
      The 14th International Symposium on Microbial Ecology (ISME14)
    • Place of Presentation
      Bella Center (Copenhagen, Denmark)
    • Related Report
      2012 Annual Research Report
  • [Presentation] Distribution of a sulfur oxidizing bacterium Sulfuricella denitrificans and proteomic insights into cold adaptation2012

    • Author(s)
      Watanabe, T.
    • Organizer
      The 14th International Symposium on Microbial Ecology (ISME14)
    • Place of Presentation
      Bella Center (Copenhagen, Denmark)
    • Related Report
      2012 Annual Research Report
  • [Presentation] Diversity of sulfate-reducing and sulfur-oxidizing organisms in sediments of Antarctic lakes2012

    • Author(s)
      Fukui, M.
    • Organizer
      SCAR Opeen Science Meeting,
    • Place of Presentation
      Hotel Hilton (Portland, USA)
    • Related Report
      2012 Annual Research Report
  • [Presentation] Spatiotemporal variations in an assemblage of closely related planktonic aerobic methane oxidizing bacteria2011

    • Author(s)
      Tsutsumi, Kojima, Fukui
    • Organizer
      FEMS2011
    • Place of Presentation
      Palexpo Geneva Congress Center(スイス)
    • Year and Date
      2011-06-28
    • Related Report
      2011 Annual Research Report
  • [Presentation] Effects of temperature on organic matter decomposition process in anaerobic marine sediment2010

    • Author(s)
      Matsui, Kojima, Fukui
    • Organizer
      第26回日本微生物生態学会
    • Place of Presentation
      つくば(茨城県)
    • Year and Date
      2010-11-25
    • Related Report
      2010 Annual Research Report
  • [Presentation] Isolation and characterization of a p-xylene-degrading sulfate-reducing bacterium2010

    • Author(s)
      Higashioka, Kojima, Fukui
    • Organizer
      第26回日本微生物生態学会
    • Place of Presentation
      つぐば(茨城県)
    • Year and Date
      2010-11-25
    • Related Report
      2010 Annual Research Report
  • [Presentation] Isolation and characterization of a novel sulfate-reducing bacterium capable of degrading p-xylene2010

    • Author(s)
      Higashioka, Kojima, Fukui
    • Organizer
      第13回国際微生物生態学シンポジウム
    • Place of Presentation
      シアトル(米国)
    • Year and Date
      2010-08-24
    • Related Report
      2010 Annual Research Report
  • [Presentation] 水界の硫黄循環システムと微生物群集

    • Author(s)
      福井学
    • Organizer
      2012年度地球惑星科学連合大会
    • Place of Presentation
      幕張メッセ(千葉県)
    • Related Report
      2012 Annual Research Report
  • [Remarks]

    • URL

      http://www.lowtem.hokudai.ac.jp/micro-ecol/MicroEcol.htm

    • Related Report
      2013 Final Research Report

URL: 

Published: 2010-08-23   Modified: 2019-07-29  

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