Elucidation of the molecular mechanism of cold stress responses in plants

• Project/Area Number: 22370014
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Plant molecular biology/Plant physiology
• Research Institution: The University of Tokyo
• Principal Investigator: YAMAGUCHI-SHINOZAKI Kazuko 東京大学, 大学院・農学生命科学研究科, 教授 (30221295)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥18,590,000 (Direct Cost: ¥14,300,000、Indirect Cost: ¥4,290,000); Fiscal Year 2012: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000); Fiscal Year 2011: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000); Fiscal Year 2010: ¥7,020,000 (Direct Cost: ¥5,400,000、Indirect Cost: ¥1,620,000)
• Keywords: 環境応答 / 低温ストレス / 発現制御 / 植物 / シス因子 / 環境ストレス応答 / 転写因子

Research Abstract

To elucidate molecular mechanisms of plant cold responses we analyzed upstream regulation of the DREB1 genes encoding transcription factors that play important roles in the cold stress responsive gene expression in plants. Previously we isolated the CAMTA family proteins as transcription factors that bind the cis-element in the DREB1C promoter that are involved in its cold stress responsive expression. We analyzed function of the CAMTA family proteins using T-DNA insertion mutants, transient transactivation system and transgenic plants expressing the GFP-fused CAMTA proteins, and found that CAMTA5 and CAMTA3 function as transcriptional activators in the expression of DREB1B and DREB1C. We thought that the cold-responsive induction of DREB1A was regulated by transcription factors other than the CAMTA proteins as transcriptional activators.

Research Products

• [Journal Article] a CCCH-tandem zinc finger protein, confers delayed senescence and stress tolerance in rice by regulating stress-related genes (2013)
  • Author(s): Asad J, Maruyama K, Todaka D, Kidokoro S, Abo M, Yoshimura E, Shinozaki K, Nakashima K,Yamaguchi-Shinozaki K.、OsTZF1
  • Journal Title: Plant Physiol Volume: 161(3) Issue: 3 Pages: 1202-16
  • DOI: 10.1104/pp.112.205385
  • Peer Reviewed
• [Journal Article] Expression of Arabidopsis DREB1C improves survival, growth, and yield of upland New Rice for Africa (NERICA) under drought.Mol. (2012)
  • Author(s): Ishizaki, T., Maruyama, K., Obara, M.,Fukutani, A., Yamaguchi-Shinozaki, K., Ito, Y., Kumashiro, T
  • Journal Title: Breeding Volume: online Issue: 2 Pages: 255-264
  • DOI: 10.1007/s11032-012-9785-9
  • Peer Reviewed
• [Journal Article] Overexpression of Arabidopsi and Rice stress genes' inducible transcription factor confers drought and salinity tolerance to rice (2012)
  • Author(s): Datta, K., Baisakh, N., Ganguly, M., Krishnan, S., Yamaguchi-Shinozaki, K., Datta, S.K
  • Journal Title: Plant Biotech. J Volume: 10(5) Issue: 5 Pages: 579-86
  • DOI: 10.1111/j.1467-7652.2012.00688.x
  • Peer Reviewed
• [Journal Article] Identification of cis-acting promoter elements in cold- and dehydration-induced transcriptional pathways in Arabidopsis, rice and soybean (2012)
  • Author(s): Maruyama, K., et al
  • Journal Title: DNA Res. Volume: 9(1) Issue: 1 Pages: 37-49
  • DOI: 10.1093/dnares/dsr040
  • Peer Reviewed
• [Journal Article] Prediction oftranscriptional regulatory elements for plant hormone responses based on microarray data (2011)
  • Author(s): Yamamoto, Y.Y., Yoshioka, Y., Hyakumachi, M., Maruyama, K., Yamaguchi-Shinozaki, K., Tokizawa, M., Koyama, H.
  • Journal Title: BMC Plant Biol Volume: 11(1) Issue: 1 Pages: 39-39
  • DOI: 10.1186/1471-2229-11-39
  • Peer Reviewed
• [Journal Article] Prediction of transcriptional regulatory elements for plant hormone response's based on microarrav data (2011)
  • Author(s): Yamamoto, Y.Y., et al.
  • Journal Title: BMC Plant Biol. Volume: 11 Pages: 39-39
  • Peer Reviewed
• [Journal Article] Immunoproteomic and Two-Dimensional Difference Gel Electrophoresis Analysis of Arabidopsis Dehydration Response Element-Binding Protein 1A (DREB1A)-Transgenic Potato (2010)
  • Author(s): Nakamura, R., Satoh, R., Nakamura, R., Shimazaki, T., Kasuga, M., Yamaguchi-Shinozaki, K., Kikuchi, A., Watanabe, K.N., Teshima, R
  • Journal Title: Biological and Pharmaceutical Bulletin Volume: 33 Issue: 8 Pages: 1418-1425
  • DOI: 10.1248/bpb.33.1418
  • NAID: 130000300370
  • ISSN: 0918-6158, 1347-5215
  • Peer Reviewed
• [Journal Article] Immunoproteomic and 2D-DIGE analysis of Arabidopsis DREB1A-transgenic potato (2010)
  • Author(s): Nakamura, R., et al.
  • Journal Title: Biol.Pharm.Bull. Volume: 33 Pages: 1418-1425
  • Peer Reviewed
• [Presentation] Transcriptional regulation of PIF family genes down-regulated under drought stress conditions in Arabidopsis (2012)
  • Author(s): S. Kidokoro, M.J. Seok, D. Todaka, S. Igusa, J. Mizoi, K. Shinozaki and K. Yamaguchi-Shinozaki
  • Organizer: 10th International Congress on Plant Molecular Biology
  • Place of Presentation: Jeju, Korea
• [Presentation] Transcriptional regulation of PIF family genes down-regulated under drought stress conditions in Arabidopsis (2012)
  • Author(s): S. Kidokoro, M.J. Seok, D. Todaka, S. Igusa, J. Mizoi, K. Shinozaki and K. Yamaguchi-Shinozaki
  • Organizer: 10th International Congress on Plant Molecular Biology
  • Place of Presentation: Jeju,Korea
• [Presentation] Transcriptional regulation of the cold-inducible DREB1 byCAMTA family in Arabidopsis (2011)
  • Author(s): Kidokoro, S., Maruyama, K., Mitsuda, N., Ohme-Takagi, M., Shinozaki, K.,Yamaguchi-Shinozaki, K
  • Organizer: XXIV SPPS Congress
  • Place of Presentation: Stavanger, Norway
• [Presentation] Functional analysis of novel membrane protein family, COR413controlled by an Arabidopsis transcription factorDREB1A. (2011)
  • Author(s): Kanai, M., Maruyama, K., Yamada, K., Kidokoro, S., Shinozaki, K.,Yamaguchi-Shinozaki, K
  • Organizer: XXIV SPPS Congress
  • Place of Presentation: Stavanger, Norway
• [Presentation] シロイヌナズナの低温誘導性転写因子遺伝子DREB1の転写制御解析 (2011)
  • Author(s): 城所聡、圓山恭之進、光田展隆、高木優、篠崎一雄、篠崎和子
  • Organizer: 第52回日本植物生理学会年会
  • Place of Presentation: 仙台
• [Presentation] ロイヌナズナの転写因子DREB1Aが制御するCOR413ファミリータンパク質の機能解析 (2011)
  • Author(s): 金井要樹、圓山恭之進、山田晃嗣、城所聡、篠崎一雄、篠崎和子
  • Organizer: 第52回日本植物生理学会年会
  • Place of Presentation: 仙台
• [Presentation] Transcriptional regulation of the cold-inducible DREB1 by CAMTA family in Arabidopsis (2011)
  • Author(s): Kidokoro, S., et al.
  • Organizer: XXIV SPPS Congress
  • Place of Presentation: Stavanger, Norway
• [Presentation] Functional analysis of novel membrane protein family, COR413 controlled by an Arabidopsis transcription factor DREB1A (2011)
  • Author(s): Kanai, M., et al.
  • Organizer: XXIV SPPS Congress
  • Place of Presentation: Stavanger, Norway
• [Presentation] シロイヌナズナの低温誘導性転写因子遺伝子DREB1の転写制御解析 (2011)
  • Author(s): 城所聡, ら
  • Organizer: 第52回日本植物生理学会年会
  • Place of Presentation: 東北大学(仙台)
• [Presentation] シロイヌナズナの転写因子DREB1Aが制御するCOR413ファミリータンパク機能解析 (2011)
  • Author(s): 金井要樹, ら
  • Organizer: 第52回日本植物生理学会年会
  • Place of Presentation: 東北大学(仙台)
• [Presentation] 環境ストレス耐性作物の開発について (2010)
  • Author(s): 篠崎和子
  • Organizer: 日本学術会議公開シンポジウム「遺伝子組換え作物とその利用に向けて」
  • Place of Presentation: 東京
  • Year and Date: 2010-08-06
• [Presentation] 環境ストレス耐性作物の開発について (2010)
  • Author(s): 篠崎和子
  • Organizer: 日本学術会議公開シンポジウム「遺伝子組換え作物とその利用に向けて」
  • Place of Presentation: 日本学術会議講堂(東京)
  • Year and Date: 2010-08-06
• [Presentation] Regulatory networks of gene expression in abiotic stress response in Arabidopsis (2010)
  • Author(s): Yamaguchi-Shinozaki, K., Shinozaki, K
  • Organizer: ICAR2010
  • Place of Presentation: パシフィコ横浜(神奈川)
• [Presentation] The cis-acting elements in cold and dehydration inducible promoters of Arabidopsis and rice (2010)
  • Author(s): Maruyama, K., Mizoi, J., Kidokoro, S., Takasaki, H., Yoshida, T., Shinozaki, K., Yamaguchi-Shinozaki, K
  • Organizer: ICAR 2010
  • Place of Presentation: パシフィコ横浜(神奈川)
• [Presentation] PIF7 negatively regulates DREB1 expression under circadian control in Arabidopsis (2010)
  • Author(s): Kidokoro, S., Maruyama, K., Nakashima, K., Imura, Y., Osakabe, Y., Fujita, Y., Mizoi, J., Shinozaki, K., Yamaguchi-Shinozaki, K
  • Organizer: ICAR 2010
  • Place of Presentation: パシフィコ横浜(神奈川)
• [Presentation] Improving abiotic stress tolerance in crops (2010)
  • Author(s): Yamaguchi-Shinozaki, K
  • Organizer: Workshop Japan-Egypt "Pharmacognosy and Traditional Medicine
  • Place of Presentation: 日本科学未来館(東京)
• [Presentation] Analysis of dehydration- and cold-induced transcriptional pathways in Arabidopsis, rice and soybean via identification of cis-acting promoter elements (2010)
  • Author(s): Maruyama, K., Mizoi, J., Kidokoro, S., Takasaki, H., Yoshida, T., Matsukura, S., Shinozaki, K., Yamaguchi-Shinozaki, K
  • Organizer: Cold Spring Harbor Asia Conference:From Plant Biology to Crop Biotechnology
  • Place of Presentation: Suzhou, China
• [Presentation] PIF7 negatively regulates DREB1 expression under circadian control in Arabidopsis (2010)
  • Author(s): Kidokoro, S., Maruyama, K., Nakashima, K., Imura, Y., Osakabe, Y., Fujita, Y., Mizoi, J., Shinozaki, K., Yamaguchi-Shinozaki, K
  • Organizer: Cold Spring Harbor Asia Conference: From Plant Biology to Crop Biotechnology
  • Place of Presentation: Suzhou, China
• [Presentation] Analysis of a novel membrane protein family (2010)
  • Author(s): Kanai, M., Maruyama, K., Yamada, K.,Kidokoro, S., Shinozaki, K., Yamaguchi-Shinozaki, K
  • Organizer: COR413 controlled by an Arabidopsistranscription factor DREB1A
  • Place of Presentation: Suzhou, China
• [Presentation] Regulatory networks of gene expression in abiotic stress response in Arabidopsis (2010)
  • Author(s): Yamaguchi-Shinozaki, K., Shinozaki, K.
  • Organizer: The 21st International Conference on Arabidopsis Research (ICAR 2010)
  • Place of Presentation: パシフィコ横浜(神奈川)
• [Presentation] The cis-acting elements in cold and dehydration inducible promoters of Arabidopsis and rice (2010)
  • Author(s): Maruyama, K., et al.
  • Organizer: The 21st International Conference on Arabidopsis Research (ICAR 2010)
  • Place of Presentation: パシフィコ横浜(神奈川)
• [Presentation] PIF7 negatively regulates DREB1 expression under circadian control in Arabidopsis (2010)
  • Author(s): Kidokoro, S., et al.
  • Organizer: The 21st International Conference on Arabidopsis Research (ICAR 2010)
  • Place of Presentation: パシフィコ横浜(神奈川)
• [Presentation] Improving abiotic stress tolerance in crops (2010)
  • Author(s): Yamaguchi-Shinozaki, K.
  • Organizer: Workshop Japan-Egypt "Pharmacognosy and Traditional Medicine"
  • Place of Presentation: 日本科学未来館(東京)
• [Presentation] Analysis of dehydration-and cold-induced transcriptional pathways in Arabidopsis, rice and soybean via identification of cis-acting promoter elements (2010)
  • Author(s): Maruyama, K., et al.
  • Organizer: Cold Spring Harbor Asia Conference : From Plant Biology to Crop Biotechnology
  • Place of Presentation: Suzhou, China
• [Presentation] PIF7 negatively regulates DREB1 expression under circadian control in Arabidopsis (2010)
  • Author(s): Kidokoro, S., et al.
  • Organizer: Cold Spring Harbor Asia Conference : From Plant Biology to Crop Biotechnology
  • Place of Presentation: Suzhou, China
• [Presentation] Analysis of a novel membrane protein family, COR413 controlled by an Arabidopsis transcription factor DREB1A (2010)
  • Author(s): Kanai, M., et al.
  • Organizer: Cold Spring Harbor Asia Conference : From Plant Biology to Crop Biotechnology
  • Place of Presentation: Suzhou, China