| Budget Amount *help |
¥18,590,000 (Direct Cost: ¥14,300,000、Indirect Cost: ¥4,290,000)
Fiscal Year 2012: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2011: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000)
Fiscal Year 2010: ¥7,020,000 (Direct Cost: ¥5,400,000、Indirect Cost: ¥1,620,000)
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| Research Abstract |
To elucidate molecular mechanisms of plant cold responses we analyzed upstream regulation of the DREB1 genes encoding transcription factors that play important roles in the cold stress responsive gene expression in plants. Previously we isolated the CAMTA family proteins as transcription factors that bind the cis-element in the DREB1C promoter that are involved in its cold stress responsive expression. We analyzed function of the CAMTA family proteins using T-DNA insertion mutants, transient transactivation system and transgenic plants expressing the GFP-fused CAMTA proteins, and found that CAMTA5 and CAMTA3 function as transcriptional activators in the expression of DREB1B and DREB1C. We thought that the cold-responsive induction of DREB1A was regulated by transcription factors other than the CAMTA proteins as transcriptional activators.
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