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Molecular mechanisms underlying reacquisition of proliferation competence during dedifferentiation of plant cells

Research Project

Project/Area Number 22370015
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Plant molecular biology/Plant physiology
Research InstitutionThe University of Tokyo

Principal Investigator

SUGIYAMA Munetaka  東京大学, 大学院・理学系研究科, 准教授 (50202130)

Co-Investigator(Renkei-kenkyūsha) OHTANI Misato  理化学研究所, バイオマス工学研究プログラム, 研究員 (60435633)
Project Period (FY) 2010 – 2012
Project Status Completed (Fiscal Year 2012)
Budget Amount *help
¥18,590,000 (Direct Cost: ¥14,300,000、Indirect Cost: ¥4,290,000)
Fiscal Year 2012: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000)
Fiscal Year 2011: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000)
Fiscal Year 2010: ¥6,890,000 (Direct Cost: ¥5,300,000、Indirect Cost: ¥1,590,000)
Keywords脱分化 / 細胞増殖能 / プレ mRNA スプライシング / rRNA 生合成 / NAC 転写因子 / 温度感受性突然変異体 / サイトカイニン / シロイヌナズナ / プレmRNAスプライシング / NAC転写因子 / rRNA生合成 / 細胸増殖能 / ブロモデオキシウリジン / rRNA / snRNA
Research Abstract

The molecular genetic analysis of Arabidopsis mutants temperature-sensitive for hypocotyl dedifferentiation has shown that the levels of pre-mRNA splicing and rRNA biosynthesis factors limit the competence of plant cell proliferation, whose reacquisition during dedifferentiation involves increase of these factors, that this control of cell proliferation is meditated by the suppression of proliferation by a specific NAC transcription factor, and that the capacity of pre-mRNA splicing required for cell proliferation varies dependently on cytokinin.

Report

(4 results)
  • 2012 Annual Research Report   Final Research Report ( PDF )
  • 2011 Annual Research Report
  • 2010 Annual Research Report
  • Research Products

    (57 results)

All 2013 2012 2011 2010 Other

All Journal Article (7 results) (of which Peer Reviewed: 7 results) Presentation (46 results) (of which Invited: 1 results) Remarks (4 results)

  • [Journal Article] Arabidopsis ROOT INITIATION DEFECTIVE 1, a DEAH-box RNA helicase involved in pre-mRNA splicing, is essential for plant development2013

    • Author(s)
      Misato Ohtani, Taku Demura, Munetaka Sugiyama
    • Journal Title

      The Plant Cell

      Volume: 25(印刷中) Issue: 6 Pages: 2056-2069

    • DOI

      10.1105/tpc.113.111922

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] How do 'housekeeping genes' control organogenesis? -unexpected new findings on the role of housekeeping genes in cell and organ differentiation.2013

    • Author(s)
      Hirokazu Tsukaya
    • Journal Title

      Journal of Plant Research

      Volume: 126 Issue: 1 Pages: 3-15

    • DOI

      10.1007/s10265-012-0518-2

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Kinematic study of root elongation in Arabidopsis thaliana with a novel image-analysis program2013

    • Author(s)
      Akitoshi Iwamoto, Eri Kondo, Hirotomo Fujihashi, Munetaka Sugiyama
    • Journal Title

      Journal of Plant Research

      Volume: 126 Issue: 1 Pages: 187-192

    • DOI

      10.1007/s10265-012-0523-5

    • NAID

      10031145628

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Tissue organization of fasciated lateral roots of Arabidopsis mutants suggestive of the robust nature of outer layer patterning2012

    • Author(s)
      Kurataka Otsuka, Munetaka Sugiyama
    • Journal Title

      Journal of Plant Research

      Volume: 125 Issue: 4 Pages: 547-554

    • DOI

      10.1007/s10265-011-0471-5

    • NAID

      10030803960

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A missense mutation in the glucosamine-6-phosphate N-acetyltransferase-encoding gene causes temperature-dependent growth defects and ectopic lignin deposition in Arabidopsis.2012

    • Author(s)
      Mamoru Nozaki
    • Journal Title

      The Plant Cell

      Volume: 24 Issue: 8 Pages: 3366-3379

    • DOI

      10.1105/tpc.112.102806

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Genetic identification of Arabidopsis RID2 as an essential factor involved in pre-rRNA processing2011

    • Author(s)
      Iwai Ohbayashi, Mineko Konishi, Kazuo Ebine, Munetaka Sugiyama
    • Journal Title

      The Plant Journal

      Volume: 67 Issue: 1 Pages: 49-60

    • DOI

      10.1111/j.1365-313x.2011.04574.x

    • Related Report
      2012 Final Research Report 2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Particular significance of SRD2-dependent snRNA accumulation in polarized pattern generation during lateral root development of Arabidopsis.2010

    • Author(s)
      Misato Ohtani
    • Journal Title

      Plant & Cell Physiology

      Volume: 51 Pages: 2002-2012

    • NAID

      10028056369

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Presentation] 側根原基形成時の細胞分裂制御におけるミトコンドリアmRNA代謝の役割2013

    • Author(s)
      杉山 宗隆
    • Organizer
      第54回日本植物生理学会年会
    • Place of Presentation
      岡山大学(岡山)
    • Related Report
      2012 Annual Research Report
  • [Presentation] NAC転写因子の機能欠損によるas2 rpl4dが示す葉の背軸化の抑制2013

    • Author(s)
      堀口 吾朗
    • Organizer
      第54回日本植物生理学会年会
    • Place of Presentation
      岡山大学(岡山)
    • Related Report
      2012 Annual Research Report
  • [Presentation] リボソーム機能の不全はNAC転写因子ANAC082を介して発生過程に影響する2013

    • Author(s)
      大林 祝
    • Organizer
      第54回日本植物生理学会年会
    • Place of Presentation
      岡山大学(岡山)
    • Related Report
      2012 Annual Research Report
  • [Presentation] サイトカイニン依存的な温度感受性を示すシロイヌナズナ突然変異体rrd4の解析2013

    • Author(s)
      池田 拓之
    • Organizer
      第54回日本植物生理学会年会
    • Place of Presentation
      岡山大学(岡山)
    • Related Report
      2012 Annual Research Report
  • [Presentation] Critical roles of Arabidopsis RID1, a DEAH-box RNA helicase, in organogenesis in vitro and in planta2012

    • Author(s)
      MiSato Ohtani
    • Organizer
      第53回日本植物生理学会年会
    • Place of Presentation
      京都産業大学(京都)
    • Year and Date
      2012-03-18
    • Related Report
      2011 Annual Research Report
  • [Presentation] シロイヌナズナにおける扁平で左右相称な葉の形成の仕組み:ASYMMETRIC LEAVES2(AS2)/AS1タンパク質による制御2012

    • Author(s)
      町田泰則
    • Organizer
      第53回日本植物生理学会年会
    • Place of Presentation
      京都産業大学(京都)
    • Year and Date
      2012-03-17
    • Related Report
      2011 Annual Research Report
  • [Presentation] シロイヌナズナASYMMETRIC LEAVES2遺伝子とリボソームRNA前駆体のプロセシングに関わる因子は葉の軸性の確立に必要である2012

    • Author(s)
      松村葉子
    • Organizer
      第53回日本植物生理学会年会
    • Place of Presentation
      京都産業大学(京都)
    • Year and Date
      2012-03-17
    • Related Report
      2011 Annual Research Report
  • [Presentation] シロイヌナズナの根端成長に関する数理モデルを用いた定量解析2012

    • Author(s)
      岩元明敏
    • Organizer
      第53回日本植物生理学会年会
    • Place of Presentation
      京都産業大学(京都)
    • Year and Date
      2012-03-16
    • Related Report
      2011 Annual Research Report
  • [Presentation] Cell proliferation control depending on the capacity of essential RNA processing is mediated by a NAC transcription factor in Arabidopsis2012

    • Author(s)
      Iwai Ohbayashi, Yoko Matsumura, Misato Ohtani, Hitoshi Onouchi, Yasunori Machida, Munetaka Sugiyama
    • Organizer
      23rd International Conference on Arabidopsis Research
    • Place of Presentation
      Vienna, Austria
    • Related Report
      2012 Final Research Report
  • [Presentation] Critical roles of RID1, a DEAH-box RNA helicase involved in pre-mRNA splicing, for organogenesis in vitro and in planta2012

    • Author(s)
      Misato Ohtani
    • Organizer
      23rd International Conference on Arabidopsis Research
    • Place of Presentation
      Hofburg Imperial Palace (Vienna, Austria)
    • Related Report
      2012 Annual Research Report
  • [Presentation] Role of mitochondria in the restriction of formative cell division at the initial stage of lateral root primordium development2012

    • Author(s)
      Munetaka Sugiyama
    • Organizer
      23rd International Conference on Arabidopsis Research
    • Place of Presentation
      Hofburg Imperial Palace (Vienna, Austria)
    • Related Report
      2012 Annual Research Report
  • [Presentation] Cell proliferation control depending on the capacit of essential RNA processing is mediated by a NAC transcription factor in Arabidopsis2012

    • Author(s)
      Iwai Ohbayashi
    • Organizer
      23rd International Conference on Arabidopsis Research
    • Place of Presentation
      Hofburg Imperial Palace (Vienna, Austria)
    • Related Report
      2012 Annual Research Report
  • [Presentation] 顕微鏡画像を用いた根端成長の数理モデル解析2012

    • Author(s)
      岩元 明敏
    • Organizer
      日本植物学会 第76回大会
    • Place of Presentation
      兵庫県立大学(姫路)
    • Related Report
      2012 Annual Research Report
  • [Presentation] シロイヌナズナ温度感受性突然変異体lignescensにおける異常なリグニン蓄積について2012

    • Author(s)
      野崎 守
    • Organizer
      日本植物学会 第76回大会
    • Place of Presentation
      兵庫県立大学(姫路)
    • Related Report
      2012 Annual Research Report
  • [Presentation] rpl4d as2の葉が背軸化する表現型を抑圧するszk1-D変異株の解析2012

    • Author(s)
      島田 浩貴
    • Organizer
      日本植物学会 第76回大会
    • Place of Presentation
      兵庫県立大学(姫路)
    • Related Report
      2012 Annual Research Report
  • [Presentation] シロイヌナズナASYMMETRIC LEAVES2遺伝子とリボソームRNA前駆体のプロセシング因子の不全が葉の向背軸性の確立にもたらす影響2012

    • Author(s)
      松村 葉子
    • Organizer
      日本植物学会 第76回大会
    • Place of Presentation
      兵庫県立大学(姫路)
    • Related Report
      2012 Annual Research Report
  • [Presentation] 道管細胞への分化運命を左右するキナーゼの解析2012

    • Author(s)
      近藤 侑貴
    • Organizer
      日本植物学会 第76回大会
    • Place of Presentation
      兵庫県立大学(姫路)
    • Related Report
      2012 Annual Research Report
  • [Presentation] シロイヌナズナのNAC転写因子ANAC082はrRNA生合成の異常時に細胞増殖を抑制する2012

    • Author(s)
      大林 祝
    • Organizer
      日本植物学会 第76回大会
    • Place of Presentation
      兵庫県立大学(姫路)
    • Related Report
      2012 Annual Research Report
  • [Presentation] 葉序の基本定理について2012

    • Author(s)
      杉山 宗隆
    • Organizer
      日本植物学会 第76回大会
    • Place of Presentation
      兵庫県立大学(姫路)
    • Related Report
      2012 Annual Research Report
  • [Presentation] Involvement of mitochondrial mRNA metabolism in the restriction of formative cell division at the initial stage of lateral root primordium development2012

    • Author(s)
      Munetaka Sugiyama
    • Organizer
      Frontiers in Plant RNA Research 2012
    • Place of Presentation
      北海道大学(札幌)
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Presentation] Cell proliferation control depending on the capacity of essential RNA processing is mediated by a NAC transcription factor in Arabidopsis2011

    • Author(s)
      Iwai Ohbayashi
    • Organizer
      第34回日本分子生物学会年会
    • Place of Presentation
      パシフィコ横浜(横浜)
    • Year and Date
      2011-12-16
    • Related Report
      2011 Annual Research Report
  • [Presentation] シロイヌナズナ葉ディスクを用いた木部・師部分化系の確立2011

    • Author(s)
      近藤侑貴
    • Organizer
      第75回日本植物学会大会
    • Place of Presentation
      東京大学(東京)
    • Year and Date
      2011-09-19
    • Related Report
      2011 Annual Research Report
  • [Presentation] 側根形成初期における細胞分裂域の制御機構:RNA代謝系の関与の可能性2011

    • Author(s)
      大塚蔵嵩
    • Organizer
      第75回日本植物学会大会
    • Place of Presentation
      東京大学(東京)
    • Year and Date
      2011-09-17
    • Related Report
      2011 Annual Research Report
  • [Presentation] ゲノム倍数化かシロイヌナズナの根端成長に及ぼす影響の解析2011

    • Author(s)
      近藤衣里
    • Organizer
      第75回日本植物学会大会
    • Place of Presentation
      東京大学(東京)
    • Year and Date
      2011-09-17
    • Related Report
      2011 Annual Research Report
  • [Presentation] NAC型転写因子ANAC082を介した細胞増殖の負の制御2011

    • Author(s)
      大林祝
    • Organizer
      第75回日本植物学会大会
    • Place of Presentation
      東京大学(東京)
    • Year and Date
      2011-09-17
    • Related Report
      2011 Annual Research Report
  • [Presentation] 細胞分裂再開過程におけるpre-mRNAスプライシング活性制御2011

    • Author(s)
      大谷美沙都
    • Organizer
      第75回日本植物学会大会
    • Place of Presentation
      東京大学(東京)
    • Year and Date
      2011-09-17
    • Related Report
      2011 Annual Research Report
  • [Presentation] Differential requirement for the supply of essential RNAs, such as snRNAs and rRNAs, in various aspects of plant development2011

    • Author(s)
      Munetaka Sugiyama
    • Organizer
      XVIII International Botanical Congress
    • Place of Presentation
      MCEC (Melbourne, Australia)
    • Year and Date
      2011-07-28
    • Related Report
      2011 Annual Research Report
  • [Presentation] Phenotypic and molecular characterization of temperature-sensitive mutants of Arabidopsis that form fasciated roots under the restrictive temperature2011

    • Author(s)
      Kurataka Otsuka
    • Organizer
      XVIII International Botanical Congress
    • Place of Presentation
      MCEC (Melbourne, Australia)
    • Year and Date
      2011-07-28
    • Related Report
      2011 Annual Research Report
  • [Presentation] Analysis of lignescens, a novel temperature-sensitive mutant of Arabidopsis exhibiting ectopic lignin deposition2011

    • Author(s)
      Mamoru Nozaki
    • Organizer
      XVIII International Botanical Congress
    • Place of Presentation
      MCEC (Melbourne, Australia)
    • Year and Date
      2011-07-28
    • Related Report
      2011 Annual Research Report
  • [Presentation] シロイヌナズナASYMMETRIC LEAVES2遺伝子とrRNA前駆体のプロセシングに関わるDEAD box RNA helicaseの変異は葉の軸性の確立に影響する2011

    • Author(s)
      松村葉子
    • Organizer
      第52回日本植物生理学会年会
    • Place of Presentation
      東北大学(仙台)
    • Year and Date
      2011-03-22
    • Related Report
      2010 Annual Research Report
  • [Presentation] 制限温度下で帯化根を形成するシロイヌナズナ温度感受性変異体rrd1、rrd2、rid4の解析2011

    • Author(s)
      杉山宗隆
    • Organizer
      第52回日本植物生理学会年会
    • Place of Presentation
      東北大学(仙台)
    • Year and Date
      2011-03-21
    • Related Report
      2010 Annual Research Report
  • [Presentation] ゲノム倍数化がシロイヌナズナの根端成長へ及ぼす影響の定量的比較解析2011

    • Author(s)
      近藤衣里
    • Organizer
      第52回日本植物生理学会年会
    • Place of Presentation
      東北大学(仙台)
    • Year and Date
      2011-03-20
    • Related Report
      2010 Annual Research Report
  • [Presentation] Differential requirement for the supply of essential RNAs, such as snRNAs and rRNAs, in various aspects of plant development2011

    • Author(s)
      Munetaka Sugiyama, Iwai Ohbayashi, MisatoOhtani
    • Organizer
      XVIII International Botanical Congress
    • Place of Presentation
      Melbourne, Australia
    • Related Report
      2012 Final Research Report
  • [Presentation] シロイヌナズナasymmetric leavesl (as1)及びas2変異体背景で向背軸形成に異常を生じる変異体の解析2010

    • Author(s)
      林里香
    • Organizer
      日本植物学会第74回大会
    • Place of Presentation
      中部大学(春日井)
    • Year and Date
      2010-09-11
    • Related Report
      2010 Annual Research Report
  • [Presentation] 制限温度下で異所性リグニン沈着を示すシロイヌナズナ温度感受性突然変異体lignescensの原因遺伝子2010

    • Author(s)
      野崎守
    • Organizer
      日本植物学会第74回大会
    • Place of Presentation
      中部大学(春日井)
    • Year and Date
      2010-09-11
    • Related Report
      2010 Annual Research Report
  • [Presentation] シロイヌナズナ側根形成におけるSRD2依存的なsnRNA蓄積の重要性2010

    • Author(s)
      大谷美沙都
    • Organizer
      日本植物学会第74回大会
    • Place of Presentation
      中部大学(春日井)
    • Year and Date
      2010-09-10
    • Related Report
      2010 Annual Research Report
  • [Presentation] ASYMMETRIC LEAVES2(AS2)とrRNAのプロセッシングに関わる因子の変異は葉の向背軸性の確立に影響する2010

    • Author(s)
      松村葉子
    • Organizer
      日本植物学会第74回大会
    • Place of Presentation
      中部大学(春日井)
    • Year and Date
      2010-09-10
    • Related Report
      2010 Annual Research Report
  • [Presentation] シロイヌナズナ2倍体と4倍体の根端成長に関する定量的比較解析2010

    • Author(s)
      近藤衣里
    • Organizer
      日本植物学会第74回大会
    • Place of Presentation
      中部大学(春日井)
    • Year and Date
      2010-09-09
    • Related Report
      2010 Annual Research Report
  • [Presentation] 脱分化・細胞増殖再活性化に異常を示すシロイヌナズナ変異体rid2とその抑圧変異sriwlの解析2010

    • Author(s)
      大林祝
    • Organizer
      日本植物学会第74回大会
    • Place of Presentation
      中部大学(春日井)
    • Year and Date
      2010-09-09
    • Related Report
      2010 Annual Research Report
  • [Presentation] Analysis of the inhibitory action of 5-bromo-2'-deoxyuridine against dedifferentiation of Arabidopsis thaliana.2010

    • Author(s)
      Kenro Imamura
    • Organizer
      21st International Conference on Arabidopsis Research
    • Place of Presentation
      パシフィコ横浜(横浜)
    • Year and Date
      2010-06-08
    • Related Report
      2010 Annual Research Report
  • [Presentation] Significance of pre-mRNA splicing capacity control in dedifferentiation and organogenesis in Arabidopsis.2010

    • Author(s)
      Misato Ohtani
    • Organizer
      21st International Conference on Arabidopsis Research
    • Place of Presentation
      パシフィコ横浜(横浜)
    • Year and Date
      2010-06-08
    • Related Report
      2010 Annual Research Report
  • [Presentation] Kinematic analysis of ploidy effects on root apical growth in Arabidopsis thaliana.2010

    • Author(s)
      Akitoshi Iwamoto
    • Organizer
      21st International Conference on Arabidopsis Research
    • Place of Presentation
      パシフィコ横浜(横浜)
    • Year and Date
      2010-06-08
    • Related Report
      2010 Annual Research Report
  • [Presentation] Requirement for the RID3-dependent negative control of cell proliferation in de novomornhogenesis.2010

    • Author(s)
      Munetaka Sugiyama
    • Organizer
      21st International Conference on Arabidopsis Research
    • Place of Presentation
      パシフィコ横浜(横浜)
    • Year and Date
      2010-06-07
    • Related Report
      2010 Annual Research Report
  • [Presentation] Analysis of rid2, a temperature-sensitive mutant that is defective in pre-rRNA processing and in several aspects of cell proliferation, and its suppressor mutant, sriwl.2010

    • Author(s)
      Iwai Ohbayashi
    • Organizer
      21st International Conference on Arabidopsis Research
    • Place of Presentation
      パシフィコ横浜(横浜)
    • Year and Date
      2010-06-07
    • Related Report
      2010 Annual Research Report
  • [Presentation] Mutations in ASYMMETRIC LEAVES2 and a DEAD-BOX RNA HELICASE required for the rRNA processing synergistically impair the establishment of the leaf polarity in Arabidopsis thaliana.2010

    • Author(s)
      Yoko Matsumura
    • Organizer
      21st International Conference on Arabidopsis Research
    • Place of Presentation
      パシフィコ横浜(横浜)
    • Year and Date
      2010-06-07
    • Related Report
      2010 Annual Research Report
  • [Presentation] Analysis of rrd1, rrd2, and rid4, temperature-sensitive mutants of Arabidopsis that form fasciated roots under the restrictive temperature.2010

    • Author(s)
      Kurataka Otsuka
    • Organizer
      21st International Conference on Arabidopsis Research
    • Place of Presentation
      パシフィコ横浜(横浜)
    • Year and Date
      2010-06-07
    • Related Report
      2010 Annual Research Report
  • [Remarks]

    • URL

      http://www.bg.s.u-tokyo.ac.jp/koishikawa/research/sugi-lab/sugi-1.html

    • Related Report
      2012 Final Research Report
  • [Remarks] 植物生理学グループ

    • URL

      http://www.bg.s.u-tokyo.ac.jp/koishikawa/research/sugi-lab/sugi-1.html

    • Related Report
      2012 Annual Research Report
  • [Remarks]

    • URL

      http://www.bg.s.u-tokyo.ac.jp/koishikawa/research/sugi-lab/sugi-1.html

    • Related Report
      2011 Annual Research Report
  • [Remarks]

    • URL

      http://www.bg.s.u-tokyo.ac.jp/koishikawa/research/sugi-lab/sugi-1.html

    • Related Report
      2010 Annual Research Report

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Published: 2010-08-23   Modified: 2019-07-29  

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