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Mechanism for the partitioning of glycerolipid biosynthetic pathways in leaves and seeds

Research Project

Project/Area Number 22370016
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Plant molecular biology/Plant physiology
Research InstitutionTokyo Institute of Technology

Principal Investigator

OHTA Hiroyuki  東京工業大学, バイオ研究基盤支援総合センター, 教授 (20233140)

Co-Investigator(Kenkyū-buntansha) AWAI Koichiro  静岡大学, 若手グローバル研究リーダー育成拠点, 特任助教 (80431732)
SHIMOJIMA Mie  東京工業大学, バイオ研究基盤支援総合センター, 助教 (90401562)
Project Period (FY) 2010 – 2012
Project Status Completed (Fiscal Year 2012)
Budget Amount *help
¥18,460,000 (Direct Cost: ¥14,200,000、Indirect Cost: ¥4,260,000)
Fiscal Year 2012: ¥6,110,000 (Direct Cost: ¥4,700,000、Indirect Cost: ¥1,410,000)
Fiscal Year 2011: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000)
Fiscal Year 2010: ¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000)
Keywords代謝生理 / オルガネラ / 脂質 / 貯蔵脂質 / 膜脂質 / トリアシルグリセロール / 糖脂質 / ホスファチジン酸ホスファターゼ
Research Abstract

Both membrane and storage lipids are mainly composed of glycerolipids in many living organisms, which commonly contain two or three fatty acid moieties attached to a glycerol backbone. In case of higher plants, all of the fatty acids are synthesized in plastids, whereas the major sites for the membrane and storage lipid biosyntheses are spatially separated in a cell. Since each plant organ contain different ratio of the two types of the glycerolipids and the ratio itself is one of a major feature of each organ identity, it is of interest how plants control the partitioning of the two different biosynthetic pathways for the membrane and storage glycerolipids. In this project, we studied on the strict control system for the partitioning of two different pathways of membrane and storage glycerolipid biosyntheses in each plant organ. In the course of this study, we found several nutrient deficient conditions strongly enhanced TAG synthesis even in leaves, and clarified the importance of phosphatidate phosphohydrolase (PAH1/2) in the leaf TAG synthesis. Moreover, we identified novel mutants which lack suppression of chloroplast membrane biogenesis in roots.

Report

(4 results)
  • 2012 Annual Research Report   Final Research Report ( PDF )
  • 2011 Annual Research Report
  • 2010 Annual Research Report
  • Research Products

    (43 results)

All 2013 2012 2011 2010

All Journal Article (16 results) (of which Peer Reviewed: 15 results) Presentation (19 results) (of which Invited: 1 results) Book (4 results) Patent(Industrial Property Rights) (4 results)

  • [Journal Article] Role of galactolipid biosynthesis in coordinated development of photosynthetic complexes and thylakoid membranes during chloroplast biogenesis in Arabidopsis.2013

    • Author(s)
      Kobayashi K, Narise T, Sonoike K, Hashimoto H, Sato N, Kondo M, Nishimura M, Sato M, Toyooka K, Sugimoto K, Wada H, Masuda T, Ohta H. (
    • Journal Title

      Plant J.

      Volume: 73 Pages: 250-261

    • Related Report
      2012 Annual Research Report 2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] A novel serine protease with caspase- and legumain-like activities from edible mushroom Flammulina velutipes2013

    • Author(s)
      Iketani A, Nakamura M, Awai K andShioi Y
    • Journal Title

      Fungal Biology

      Volume: 117 Pages: 173-181

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] A novel serine protease with caspase- and legumain-like activities from edible mushroom Flammulina velutipes.2013

    • Author(s)
      Iketani A, Nakamura M, Awai K and Shioi Y
    • Journal Title

      Fungal Biology

      Volume: 117 Issue: 3 Pages: 173-181

    • DOI

      10.1016/j.funbio.2013.01.005

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Structural confirmation of a unique carotenoid lactoside2012

    • Author(s)
      Wakahama, T., Laza-Martínez, A.,BinHaji Mohd Taha, A.I., Okuyama, H., Yoshida, K., Kogame, K., Awai, K., Kawachi, M., Maoka, T.and Takaichi, S. (
    • Journal Title

      Journal of Phycology

      Volume: 48 Pages: 1392-1402

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Phylogeny of galactolipid synthase homologs together with their enzymatic analyses revealed a possible origin and divergence time for photosynthetic Membrane biogenesis.2012

    • Author(s)
      Yuzawa Y, Nishihara H, Haraguchi T,Masuda S, Shimojima M, Shimoyama A, Yuasa H, Okada N, Ohta H. (
    • Journal Title

      DNA Res.

      Volume: 19 Pages: 91-102

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Structural confirmation of a unique carotenoid lactoside, p457, in Symbiodinium sp. Strain NBRC 104787 isolated from a sea anemone and its distribution in dinoflagellates and various marine organisms.2012

    • Author(s)
      Wakahama, T., Laza-Martínez, A., Bin Haji Mohd Taha, A.I., Okuyama, H., Yoshida, K., Kogame, K., Awai, K., Kawachi, M., Maoka, T. and Takaichi, S.
    • Journal Title

      Journal of Phycology

      Volume: 48 Issue: 6 Pages: 1392-1402

    • DOI

      10.1111/j.1529-8817.2012.01219.x

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Phylogeny of galactolipid synthase homologs together with their enzymatic analyses revealed a possible origin and divergence time for photosynthetic Membrane biogenesis2012

    • Author(s)
      Yuzawa Y, Nishihara H, Haraguchi T, Masuda S, Shimojima M, Shimoyama A, Yuasa H, Okada N, Ohta H
    • Journal Title

      DNA Res

      Volume: 19 Issue: 1 Pages: 91-102

    • DOI

      10.1093/dnares/dsr044

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A monogalactosyldiacylglycerol synthase found in the green sulfur bacterium Chlorobaculum tepidum reveals important roles for galactolipids in photosynthesis2011

    • Author(s)
      Masuda S, Harada J, Yokono M, Yuzawa Y, Shimojima M, Murofushi K, Tanaka H, Masuda H, Murakawa M, Haraguchi T, Kondo M, Nishimura M, Yuasa H, Noguchi M, Oh-Oka H, Tanaka A, Tamiaki H, Ohta H. (
    • Journal Title

      Plant Cell

      Volume: 23 Pages: 2644-2658

    • Related Report
      2012 Final Research Report 2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Criticalregulation of galactolipid synthesis controls membrane differentiation and remodeling in distinct plant organs and following environmental changes.2011

    • Author(s)
      Shimojima M, Ohta H
    • Journal Title

      ProgLipid Res.

      Volume: 50 Pages: 258-266

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Biosynthesis andfunctions of the plant sulfolipid2011

    • Author(s)
      Shimojima M
    • Journal Title

      ProgLipid Res

      Volume: 50 Pages: 234-239

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Biosynthesis and functions of the plant sulfolipid2011

    • Author(s)
      Shimojima M
    • Journal Title

      Prog Lipid Res

      Volume: 50 Issue: 3 Pages: 234-239

    • DOI

      10.1016/j.plipres.2011.02.003

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Critical regulation of galactolipid synthesis controls membrane differentiation and remodeling in distinct plant organs and following environmental changes2011

    • Author(s)
      Shimojima M, Ohta H
    • Journal Title

      Prog Lipid Res

      Volume: 50 Issue: 3 Pages: 258-266

    • DOI

      10.1016/j.plipres.2011.03.001

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Involvement of auxin signaling mediated by IAA14 and ARF7/19 in membrane lipid remodeling during phosphate starvation.2010

    • Author(s)
      Narise T, Kobayashi K, BabaS, Shimojima M, Masuda S, Fukaki H, Ohta H
    • Journal Title

      Plant MolBiol

      Volume: 72 Pages: 533-544

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] 高等植物ホスファチジン酸ホスファターゼの膜脂質合成2010

    • Author(s)
      中村友輝、太田啓之
    • Journal Title

      シグナリングにおける機能生化学

      Volume: 82 Pages: 1137-1141

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Involvement of auxin signaling mediated by IAA14 and ARF7/19 in membrane lipid remodeling during phosphate starvation2010

    • Author(s)
      Narise T, Kobayashi K, Baba S, Shimojima M, Masuda S, Fukaki H, Ohta H
    • Journal Title

      Plant Mol Biol

      Volume: 72 Pages: 533-544

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 高等植物ホスファチジン酸ホスファターゼの膜脂質合成、シグナリングにおける機能2010

    • Author(s)
      中村友輝、太田啓之
    • Journal Title

      生化学

      Volume: 82 Pages: 1137-1141

    • NAID

      10027880277

    • Related Report
      2010 Annual Research Report
  • [Presentation] Metabolic remodeling of glycerolipid synthesis in response to developmental and environmental changes Gordon Research Conference2013

    • Author(s)
      Hiroyuki Ohta
    • Organizer
      Plant lipid: Structure, Metabolism & Function 2011
    • Place of Presentation
      Galveston TX, USA
    • Year and Date
      2013-01-29
    • Related Report
      2012 Final Research Report
  • [Presentation] Galactolipid synthesis "Mechanism and Evolution"2013

    • Author(s)
      Hiroyuki Ohta
    • Organizer
      Gordon Research Conference "Plant Lipids:Structure, Metabolism & Function"
    • Place of Presentation
      Galveston (USA)
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Presentation] リン欠乏時の膜脂質転換にかかわる新規因子の探索2013

    • Author(s)
      園部耕平、成瀬孝史、沼田光紗、下嶋美恵、太田啓之
    • Organizer
      第54回植物生理学会年会
    • Place of Presentation
      岡山大学津島キャンパス (岡山)
    • Related Report
      2012 Annual Research Report
  • [Presentation] シロイヌナズナ変異体を用いたホスファチジン酸ホスホヒドロラーゼの機能解析2012

    • Author(s)
      駿河航
    • Organizer
      第53回日本植物生理学会年会
    • Place of Presentation
      京都
    • Year and Date
      2012-03-18
    • Related Report
      2011 Annual Research Report
  • [Presentation] リン欠乏生育条件下でのシロイヌナズナ葉における貯蔵脂質蓄積の解析2012

    • Author(s)
      下嶋美恵
    • Organizer
      第53回日本植物生理学会年会
    • Place of Presentation
      京都
    • Year and Date
      2012-03-16
    • Related Report
      2011 Annual Research Report
  • [Presentation] シロイヌナズナMGDG合成酵素過剰発現体の解析2012

    • Author(s)
      下村雄一
    • Organizer
      第53回日本植物生理学会年会
    • Place of Presentation
      京都
    • Year and Date
      2012-03-16
    • Related Report
      2011 Annual Research Report
  • [Presentation] 葉緑体糖脂質合成酵素MGD3過剰発現体の解析2012

    • Author(s)
      村川雅人、下村雄一、小林康一、粟井光一郎、下嶋美恵、太田啓之
    • Organizer
      第25回植物脂質シンポジウム
    • Place of Presentation
      甲南大学 (兵庫)
    • Related Report
      2012 Annual Research Report
  • [Presentation] HISTOCHEMICAL ANALYSIS OF MGD1, THE GENE RESPONSIBLE FOR BULK SYNTHESIS OF MGDG2011

    • Author(s)
      山崎尭嗣
    • Organizer
      4th Asian Symposium on Plant Lipids
    • Place of Presentation
      香港
    • Year and Date
      2011-12-03
    • Related Report
      2011 Annual Research Report
  • [Presentation] Histochemical analysis of MGD1, the gene responsible for bulk synthesis of monogalactosyldiacylglycerol2011

    • Author(s)
      山崎尭嗣
    • Organizer
      Shizuoka University International Symposium 2011
    • Place of Presentation
      静岡
    • Year and Date
      2011-11-28
    • Related Report
      2011 Annual Research Report
  • [Presentation] 光合成膜主要糖脂質合成酵素の遺伝子発現制御2011

    • Author(s)
      山崎尭嗣
    • Organizer
      第24回植物脂質シンポジウム
    • Place of Presentation
      東京
    • Year and Date
      2011-09-20
    • Related Report
      2011 Annual Research Report
  • [Presentation] 光合成膜主要糖脂質合成酵素の遺伝子発現制御2011

    • Author(s)
      山崎尭嗣
    • Organizer
      第75回日本植物学会年会
    • Place of Presentation
      東京
    • Year and Date
      2011-09-18
    • Related Report
      2011 Annual Research Report
  • [Presentation] リン欠乏生育条件下でのシロイヌナズナ葉における貯蔵脂質蓄積の解析2011

    • Author(s)
      下嶋美恵
    • Organizer
      第52回日本植物生理学会年会
    • Place of Presentation
      仙台
    • Year and Date
      2011-03-20
    • Related Report
      2010 Annual Research Report
  • [Presentation] シロイヌナズナのホスファチジン酸ホスファターゼ変異体におけるストレス応答の解析2011

    • Author(s)
      沼田光紗
    • Organizer
      第52回日本植物生理学会年会
    • Place of Presentation
      仙台
    • Year and Date
      2011-03-20
    • Related Report
      2010 Annual Research Report
  • [Presentation] 光合成膜主要糖脂質合成酵素遺伝子MGD1の組織化学的発現解析2011

    • Author(s)
      山崎尭嗣
    • Organizer
      第12回静岡ライフサイエンスシンポジウム
    • Place of Presentation
      静岡
    • Year and Date
      2011-03-04
    • Related Report
      2010 Annual Research Report
  • [Presentation] Metabolic remodeling of glycerolipid synthesis in response to developmental and environmental changes2011

    • Author(s)
      Hiroyuki Ohta
    • Organizer
      Gordon Research Conference
    • Place of Presentation
      米国・テキサス
    • Year and Date
      2011-01-31
    • Related Report
      2010 Annual Research Report
  • [Presentation] シロイヌナズナ種子貯蔵脂質生合成におけるホスファチジン酸ホスファターゼの役割2010

    • Author(s)
      下嶋美恵
    • Organizer
      第23回植物脂質シンポジウム
    • Place of Presentation
      京都
    • Year and Date
      2010-11-27
    • Related Report
      2010 Annual Research Report
  • [Presentation] ABAを介したストレス応答におけるシロイヌナズナPAH1/2の役割2010

    • Author(s)
      沼田光紗
    • Organizer
      第23回植物脂質シンポジウム
    • Place of Presentation
      京都
    • Year and Date
      2010-11-27
    • Related Report
      2010 Annual Research Report
  • [Presentation] リン欠乏時の膜脂質転換におけるホスファチジン酸生成・分解機構の解析2010

    • Author(s)
      駿河航
    • Organizer
      第23回植物脂質シンポジウム
    • Place of Presentation
      京都
    • Year and Date
      2010-11-27
    • Related Report
      2010 Annual Research Report
  • [Presentation] Multiple functions of phosphatidate phosphatases in glycerolipid biosynthesis2010

    • Author(s)
      Hiroyuki Ohta
    • Organizer
      19^<th> International Symposium on Plant Lipids
    • Place of Presentation
      オーストラリア・ケアンズ
    • Year and Date
      2010-07-15
    • Related Report
      2010 Annual Research Report
  • [Book] Molecular Biology and Bioengineering Springer-Verlag2010

    • Author(s)
      Nakamura Y, Kobayashi K, Shimojima M and Ohta H
    • Publisher
      The Chloroplast Biochemistry
    • Related Report
      2012 Final Research Report
  • [Book] Lipid Signaling in Plants Springer-Verlag Berlin Heidelberg2010

    • Author(s)
      Nakamura Y, and Ohta H
    • Related Report
      2012 Final Research Report
  • [Book] The Chloroplast Biochemistry, Molecular Biology and Bioengineering2010

    • Author(s)
      Nakamura Y, Kobayashi K, Shimojima M, Ohta H
    • Publisher
      Springer-Verlag
    • Related Report
      2010 Annual Research Report
  • [Book] Lipid Signaling in Plants2010

    • Author(s)
      Nakamura Y, Ohta H
    • Publisher
      Springer-Verlag Berlin Heidelberg
    • Related Report
      2010 Annual Research Report
  • [Patent(Industrial Property Rights)] 植物油脂の製造法2011

    • Inventor(s)
      嶋美恵、円由香、太田啓之、遠藤 圭二
    • Industrial Property Rights Holder
      東京工業大学、花王株式会社
    • Filing Date
      2011-01-28
    • Related Report
      2012 Final Research Report
  • [Patent(Industrial Property Rights)] 植物油脂の製造法2011

    • Inventor(s)
      下嶋美恵、円由香、太田啓之、遠藤圭二
    • Industrial Property Rights Holder
      東京工業大学、花王株式会社
    • Industrial Property Number
      2011-016848
    • Filing Date
      2011-01-28
    • Related Report
      2010 Annual Research Report
  • [Patent(Industrial Property Rights)] 植物油脂の製造法2010

    • Inventor(s)
      太田啓之、下嶋美恵、小泉遼太、遠 藤圭二
    • Industrial Property Rights Holder
      東京工業大学、花王株式会社
    • Filing Date
      2010-05-28
    • Related Report
      2012 Final Research Report
  • [Patent(Industrial Property Rights)] 植物油脂の製造法2010

    • Inventor(s)
      太田啓之、下嶋美恵、小泉遼太、遠藤圭二
    • Industrial Property Rights Holder
      東京工業大学、花王株式会社
    • Industrial Property Number
      2010-123162
    • Filing Date
      2010-05-28
    • Related Report
      2010 Annual Research Report

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Published: 2010-08-23   Modified: 2019-07-29  

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