Elucidation of regulatory system for secondary cell wall formation

• Project/Area Number: 22370020
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Plant molecular biology/Plant physiology
• Research Institution: Nara Institute of Science and Technology
• Principal Investigator: DEMURA Taku 奈良先端科学技術大学院大学, バイオサイエンス研究科, 教授 (40272009)
• Co-Investigator(Renkei-kenkyūsha): YAMAGUCHI Masatoshi 埼玉大学, 環境科学研究センター, 准教授 (20373376)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥18,980,000 (Direct Cost: ¥14,600,000、Indirect Cost: ¥4,380,000); Fiscal Year 2012: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2011: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2010: ¥10,660,000 (Direct Cost: ¥8,200,000、Indirect Cost: ¥2,460,000)
• Keywords: 木質バイオマス / 二次細胞壁 / VND7 / セルロース合成 / ヘミセルロース合成 / タバコBY-2 / シロイヌナズナ / 共発現解析 / 細胞壁 / オルガネラ / キシラン / リン酸化 / セルロース

Research Abstract

Secondary cell walls (SCWs) of plants are important because they are major components of woody biomass. However, little is known about mechanisms underlying SCW formation. In this study, we revealed several new aspects of the mechanisms through the analysis of biosynthesis of cellulose and xylan, major constituents of SCWs, and the analysis of regulatory system of VND7, the master regulatory switch of SCW formation, by using a new in vitro SCW induction experimental system that was established with the VND7 gene.

Research Products

• [Journal Article] Comparative metabolome analysis of seed kernels in phorbol ester-containing and phorbol ester-free accessions of Jatropha curcas L (2012)
  • Author(s): Ohtani, M., Nakano, Y., Usami, T.,Demura, T.
  • Journal Title: Plant Biotechnol Volume: 29 Pages: 171-174
  • NAID: 10030804344
  • Peer Reviewed
• [Journal Article] Characterization of the casbene synthase homolog from Jatropha (<i>Jatropha curcas</i> L.) (2012)
  • Author(s): Nakano, Y., Ohtani, M., Polsri, W.,Usami, T., Sambongi, K., Demura, T.
  • Journal Title: Plant Biotechnology Volume: 29 Issue: 2 Pages: 185-189
  • DOI: 10.5511/plantbiotechnology.12.0418a
  • NAID: 10030804406
  • ISSN: 1342-4580, 1347-6114
  • Peer Reviewed
• [Journal Article] Comparative metabolome analysis of seed kernels in phorbol ester-containing and phorbol ester-free accessions of <i>Jatropha curcas</i> L. (2012)
  • Author(s): Ohtani, M 他
  • Journal Title: Plant Biotechnology Volume: 29 Issue: 2 Pages: 171-174
  • DOI: 10.5511/plantbiotechnology.12.0426a
  • NAID: 10030804344
  • ISSN: 1342-4580, 1347-6114
  • Peer Reviewed
• [Journal Article] VASCULAR-RELATED NAC-DOMAIN7 directly regulates the expression of a broad range of genes for xylem vessel formation (2011)
  • Author(s): Yamaguchi, M., Mitsuda, N., Ohtani, M., Ohme-Takagi, M., Kato, K., and Demura, T
  • Journal Title: Plant J Volume: 66 Issue: 4 Pages: 579-590
  • DOI: 10.1111/j.1365-313x.2011.04514.x
  • Peer Reviewed
• [Journal Article] A NAC domain protein family contributing to the regulation of wood formation in poplar (2011)
  • Author(s): Ohtani, M., Nishikubo, N., Xu B., Yamaguchi, M., Mitsuda, N., and Goue, N., Shi, F., Ohme-Takagi, M., and Demura, T.
  • Journal Title: Plant J Volume: 67 Issue: 3 Pages: 499-512
  • DOI: 10.1111/j.1365-313x.2011.04614.x
  • Peer Reviewed
• [Journal Article] The CKH2/PKL Chromatin Remodeling Factor Negatively Regulates Cytokinin Responses in Arabidopsis Calli. (2011)
  • Author(s): Furuta, K., Kakimoto, T., et al.
  • Journal Title: Plant Cell Physiol. Volume: 52 Issue: 4 Pages: 618-628
  • DOI: 10.1093/pcp/pcr022
  • NAID: 10029710051
  • Peer Reviewed
• [Journal Article] The CKH1/EER4 Gene Encoding a TAF12-Like Protein Negatively Regulates Cytokinin Sensitivity in Arabidopsis thaliana. (2011)
  • Author(s): Kubo, M., Kakimoto, t., et al.
  • Journal Title: Plant Cell Physiol. Volume: 52 Issue: 4 Pages: 629-637
  • DOI: 10.1093/pcp/pcr021
  • NAID: 10029710104
  • Peer Reviewed
• [Journal Article] Mutations in MYB3R1 and MYB3R4 cause pleiotropic developmental defects and preferential down-regulation of multiple G2/M-specific genes in Arabidopsis (2011)
  • Author(s): Haga, N, 他
  • Journal Title: Plant Physiology Volume: 157 Pages: 706-717
  • Peer Reviewed
• [Journal Article] VASCULAR-RELATEDNAC-DOMAIN6 and VASCULARRELATEDNAC-DOMAIN7 effectivelyinduce transdifferentiation into xylemvessel elements under control of aninduction system (2010)
  • Author(s): Yamaguchi, M., Goue, N., Igarashi, H.,Ohtani, M., Nakano, Y., Mortimer, J.C.,Nishikubo, N., Kubo, M., Katayama, Y.,Kakegawa, K., Dupree, P., Demura, T.
  • Journal Title: Plant Physiol Volume: 153 Issue: 3 Pages: 915-924
  • DOI: 10.1104/pp.110.154013
  • Peer Reviewed
• [Journal Article] VND-INTERACTING2, a NAC domain transcription factor, negatively regulates xylem vessel formation in Arabidopsis (2010)
  • Author(s): Yamaguchi, M., Ohtani, M., Mitsuda, N., Kubo, M., Ohme-Takagi, M., Fukuda, H., and Demura, T.
  • Journal Title: Plant Cell Volume: 22 Issue: 4 Pages: 1249-1263
  • DOI: 10.1105/tpc.108.064048
  • Peer Reviewed
• [Journal Article] Regulation ofplant biomass production. (2010)
  • Author(s): Demura, T., Ye, Z.H.
  • Journal Title: Curr. Opin.Plant Biol Volume: 13 Issue: 3 Pages: 299-304
  • DOI: 10.1016/j.pbi.2010.03.002
  • Peer Reviewed
• [Journal Article] Regulatory Mechanisms forSpecification and Patterning of PlantVascular Tissues (2010)
  • Author(s): Cano-Delgado, A., Lee, J.-Y., Demura, T.
  • Journal Title: Annu. Rev. Cell Dev. Biol. Volume: 26 Issue: 1 Pages: 605-637
  • DOI: 10.1146/annurev-cellbio-100109-104107
  • Peer Reviewed
• [Journal Article] MYB transcriptionfactors orchestrating the developmentalprogram of xylem vessels in Arabidopsisroots (2010)
  • Author(s): Nakano, Y., Nishikubo, N., Goue, N.,Ohtani, M., Yamaguchi, M., Katayama, Y.,Demura, T.
  • Journal Title: Plant Biotechnol Volume: 27 Pages: 267-272
  • URL: http://www.jspcmb.jp/pbcontents/pdf/pb27_3/27_267.pdf
  • Peer Reviewed
• [Journal Article] ANGUSTIFOLIA3 plays roles in adaxial.abaxial patterning and growth in leaf morphogenesis (2010)
  • Author(s): Horiguchi, G., 他
  • Journal Title: Plant Cell Physiology Volume: 52 Pages: 112-124
  • Peer Reviewed
• [Journal Article] Cobtorin target analysis reveals that pectin functions in the deposition of cellulose microfibrils in parallel with cortical microtubules (2010)
  • Author(s): Yoneda, A., 他
  • Journal Title: The Plant Journal Volume: 64 Pages: 657-667
  • Peer Reviewed
• [Journal Article] Particular significance of SRD2-dependent snRNA accumulation in polarized pattern generation during lateral root development of Arabidopsis. (2010)
  • Author(s): Ohtani, M., 他
  • Journal Title: Plant Cell Physiology Volume: 51 Pages: 2002-2012
  • Peer Reviewed
• [Journal Article] Regulatory mechanisms for specification and patterning of plant vascular tissues (2010)
  • Author(s): Cano-Delgado, A, 他
  • Journal Title: Annu.Rev.Cell Dev.Biol Volume: 26
  • Peer Reviewed
• [Journal Article] Regulation of plant biomass production (2010)
  • Author(s): Demura, T., 他
  • Journal Title: Curr, Opin.Plant Biol. Volume: 13 Pages: 299-304
  • Peer Reviewed
• [Journal Article] Transcriptional regulation of secondary wall formation controlled by NAC domain proteins (2010)
  • Author(s): Yamaguchi, M., 他
  • Journal Title: Plant Biotechnology Volume: 27 Pages: 237-242
  • NAID: 10026527497
  • Peer Reviewed
• [Presentation] Evolutionary conservationand divergence of genes regulatingdevelopment of water conducting cells (2013)
  • Author(s): Demura, T
  • Organizer: 2013 Plant Winter Conference
  • Place of Presentation: 韓国・浦項市
  • Year and Date: 2013-01-25
• [Presentation] CONTROL OF SECONDARYCELL WALL FORMATION BY SEVERALDISTINCT SETS OF TRANSCRIPTIONFACTORS (2012)
  • Author(s): Demura T
  • Organizer: 23th International Conferenceon Arabidopsis Research
  • Place of Presentation: オーストリア・ウィーン
  • Year and Date: 2012-07-04
• [Presentation] 木部細胞の分化に必要な分子生物学的制御因子を科学する (2012)
  • Author(s): 出村拓
  • Organizer: 第5回木質科学シンポジウム
  • Place of Presentation: 東京
  • Year and Date: 2012-06-09
• [Presentation] Regulation of woody cell wallformation in model plants and trees (2012)
  • Author(s): Demura T
  • Organizer: UmeaRenewable Energy Meeting
  • Place of Presentation: スウェーデン・ウメオ
  • Year and Date: 2012-03-15
• [Presentation] Regulation of woody cell wall formation in model plants and trees (2012)
  • Author(s): Demura, T
  • Organizer: Umea Renewable Energy Meeting 2012
  • Place of Presentation: Umea
  • Year and Date: 2012-03-15
• [Presentation] Phosphorylation of VASCULAR-RELATED NAC-DOMAIN7 ; the master regulator for xylem vessel differentiation (2011)
  • Author(s): Ogawa, Y, 他
  • Organizer: Working on Wall 2011
  • Place of Presentation: Vancouver(複数日ポスター発表)
• [Presentation] The transcriptional networks involved in xylem vessel formation (2011)
  • Author(s): Endo, H, 他
  • Organizer: Working on Wall 2011
  • Place of Presentation: Vancouver(複数日ポスター発表)
• [Presentation] Analysis of Regulatory Network of Master Gene VASCULAR-RELATED NAC-DOMAIN 7 (VND7) for Xylem Vessel Formation (2011)
  • Author(s): Kawabe, H, 他
  • Organizer: The 4th Conference on Biosynthesis of Plant Cell Wall
  • Place of Presentation: 淡路市(複数日ポスター発表)
• [Presentation] CONTROL OF SECONDARY CELL WALL FORMATION BY SEVERAL DISTINCT SETS OF TRANSCRIPTION FACTORS
  • Author(s): Taku Demura
  • Organizer: 23th International Conference on Arabidopsis Recearch
  • Place of Presentation: オーストラリア・ウィーン
  • Invited
• [Presentation] 木部細胞の分化に必要な分子生物学的制御因子を科学する
  • Author(s): 出村拓
  • Organizer: 第5回木質科学シンポジウム
  • Place of Presentation: 東京大学
  • Invited
• [Presentation] Phosphorylation of VASCULAR-RELATED NAC-DOMAIN7, the master regulator for xylem vessel differentiation
  • Author(s): 小川宣仁
  • Organizer: 第30回日本植物細胞分子生物学会大会・シンポジウム
  • Place of Presentation: 奈良先端科学技術大学院大学
• [Presentation] Phosphorylation of VASCULAR-RELATED NAC-DOMAIN7, the master regulator for xylem vessel differentiation
  • Author(s): 小川宣仁
  • Organizer: 第54回日本植物生理学会年会
  • Place of Presentation: 岡山大学
• [Book] 「二次細胞壁形成の時空間的制御」生物の科学 遺伝 (2012)
  • Author(s): 大谷美沙都, 山口雅利, 出村拓
  • Publisher: エヌ・ティー・エス
• [Book] 「セルロース系バイオマス生産植物の分子育種技術」次世代バイオエタノール生産の技術革新と事業展開 (2010)
  • Author(s): 出村拓
  • Publisher: フロンティア出版
• [Remarks]
  • URL: http://bsw3.naist.jp/demura/