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A study on the mode of action of thermospermine in the stem growth of plants

Research Project

Project/Area Number 22370021
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Plant molecular biology/Plant physiology
Research InstitutionOkayama University

Principal Investigator

TAKAHASHI Taku  岡山大学, 自然科学研究科, 教授 (20271710)

Co-Investigator(Kenkyū-buntansha) MOTOSE Hiroyasu  岡山大学, 大学院・自然科学研究科, 准教授 (70342863)
Project Period (FY) 2010-04-01 – 2014-03-31
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥17,940,000 (Direct Cost: ¥13,800,000、Indirect Cost: ¥4,140,000)
Fiscal Year 2013: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
Fiscal Year 2012: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
Fiscal Year 2011: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
Fiscal Year 2010: ¥8,580,000 (Direct Cost: ¥6,600,000、Indirect Cost: ¥1,980,000)
Keywords植物 / 発生 / ポリアミン / 維管束 / 突然変異 / シロイヌナズナ / 発生・分化 / 分子遺伝学 / 遺伝子発現 / 翻訳制御
Research Abstract

Thermospermine functions in repressing xylem differentiation in plants and its deficiency results in excess proliferation of xylem vessels and severe dwarfism. To know the mode of action of thermospermine, we have isolated suppressor mutants of a thermospermine-requiring mutant of Arabidopsis that restore the dwarf phenotype, and revealed that sac53-d and sac56-d mutants are defective in a gene encoding ribosomal protein RACK1 and L4, respectively. We further found that thermospermine can be functionally replaced with norspermine. A chemical screening experiment revealed that thermospermine and auxin have an opposite function in xylem differentiation.

Report

(5 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Annual Research Report
  • 2011 Annual Research Report
  • 2010 Annual Research Report
  • Research Products

    (38 results)

All 2014 2013 2012 2011 2010 Other

All Journal Article (8 results) (of which Peer Reviewed: 8 results,  Open Access: 1 results) Presentation (29 results) Remarks (1 results)

  • [Journal Article] Thermospermine modulates expression of auxin-related genes in A rabidopsis2014

    • Author(s)
      Wurina Tong
    • Journal Title

      Frontiers in Plant Metabolism and Chemodiver sity

      Volume: (印刷中) Pages: 94-94

    • DOI

      10.3389/fpls.2014.00094

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Determination of polyamines in Arabidopsis thaliana by capillary electrophoresis using salicylaldehyde-5-sulfonate as a derivatizing reagent2013

    • Author(s)
      Genki Inoue, Takashi Kaneta, Toshio Takayanagi, Junichi Kakehi, Hiroyasu Motose, Taku Takahashi
    • Journal Title

      Analytical Methods

      Volume: (in press) Issue: 11 Pages: 2854-2859

    • DOI

      10.1039/c3ay26360f

    • NAID

      120005285276

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Thermospermine suppresses auxin-inducible xylem differentiation in Arabidopsis thaliana2012

    • Author(s)
      K. Yoshimoto, Y. Noutoshi, K. Hayashi, K. Shirasu, T. Takahashi and H. Motose
    • Journal Title

      Plant Signal Behav.

      Volume: 7 Issue: 8 Pages: 937-939

    • DOI

      10.4161/psb.20784

    • NAID

      120004920209

    • Related Report
      2013 Final Research Report 2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A chemical biology approach reveals an opposite action between thermospermine and auxin in xylem development in Arabidopsis thaliana2012

    • Author(s)
      K. Yoshimoto, Y. Noutoshi, K. Hayashi, K. Shirasu, T. Takahashi and H. Motose
    • Journal Title

      Plant Cell Physiol

      Volume: 53 Issue: 4 Pages: 635-645

    • DOI

      10.1093/pcp/pcs017

    • NAID

      10030653224

    • Related Report
      2013 Final Research Report 2012 Annual Research Report 2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Thermospermine is not a minor polyamine in the plant kingdom2012

    • Author(s)
      A. Takano, J.-I. Kakehi and T. Takahashi
    • Journal Title

      Plant Cell Physiol

      Volume: 53 Issue: 4 Pages: 606-616

    • DOI

      10.1093/pcp/pcs019

    • NAID

      10030652956

    • Related Report
      2013 Final Research Report 2012 Annual Research Report 2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Norspermine substitutes for thermospermine in the control of stem elongation in Arabidopsis thaliana2010

    • Author(s)
      J.-I. Kakehi, Y. Kuwashiro, H. Motose, K. Igarashi, and T. Takahashi
    • Journal Title

      FEBS Lett

      Volume: 584 Issue: 14 Pages: 3042-3046

    • DOI

      10.1016/j.febslet.2010.05.035

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Arabidopsis Qa-SNARE SYP2 proteins localized to different subcellular regions function redundantly in vacuolar protein sorting and plant development.2010

    • Author(s)
      Shirakawa, et al.
    • Journal Title

      Plant J.

      Volume: 64 Pages: 924-935

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Norspermine substitutes for thermospermine in the control of stem elongation in Arabidopsis thaliana.2010

    • Author(s)
      Kakehi, et al.
    • Journal Title

      FEBS Lett.

      Volume: 584 Pages: 3042-3046

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Presentation] サーモスペルミン合成阻害剤によるシロイヌナズナ道管分化機構の解析2014

    • Author(s)
      吉本香織・高村浩由・門田功・本瀬宏康・高橋卓
    • Organizer
      第5回日本ポリアミン学会年会
    • Place of Presentation
      銚子
    • Related Report
      2013 Annual Research Report
  • [Presentation] シロイヌナズナのSAC51 mRNAの翻訳を促進するサーモスペルミンの作用機構2014

    • Author(s)
      高野綾香・石塚壮一・蔡青々・本瀬宏康・高橋卓
    • Organizer
      第55回日本植物生理学会年会
    • Place of Presentation
      富山
    • Related Report
      2013 Annual Research Report
  • [Presentation] シロイヌナズナの道管分化に対するサーモスペルミン合成阻害剤の効果2014

    • Author(s)
      吉本香織・高村浩由・門田功・本瀬宏康・高橋卓
    • Organizer
      第55回日本植物生理学会年会
    • Place of Presentation
      富山
    • Related Report
      2013 Annual Research Report
  • [Presentation] シロイヌナズナのacl5変異に対するサプレッサー変異sac57の原因遺伝子の同定2014

    • Author(s)
      蔡青々・福島弘子・石井菜水・坂本智昭・倉田哲也・高橋卓
    • Organizer
      第55回日本植物生理学会年会
    • Place of Presentation
      富山
    • Related Report
      2013 Annual Research Report
  • [Presentation] シロイヌナズナのサーモスペルミン欠乏変異による矮性表現型を抑圧する変異sac の原因遺伝子の同定2013

    • Author(s)
      福島弘子・河野恵理・坂本智昭・本瀬宏康・倉田哲也・高橋卓
    • Organizer
      第54回日本植物生理学会年会
    • Place of Presentation
      岡山
    • Year and Date
      2013-03-21
    • Related Report
      2013 Final Research Report
  • [Presentation] サーモスペルミン合成阻害剤は道管分化を促進する2013

    • Author(s)
      本瀬宏康・吉本香織・ウリナ・懸樋潤一・高村浩由・門田功・高橋卓
    • Organizer
      第54回日本植物生理学会年会
    • Place of Presentation
      岡山
    • Year and Date
      2013-03-21
    • Related Report
      2013 Final Research Report
  • [Presentation] シロイヌナズナSAC51遺伝子のサーモスペルミンによる翻訳制御機構2013

    • Author(s)
      高野綾香・石塚壮一・石井菜水・高橋卓
    • Organizer
      第54回日本植物生理学会年会
    • Place of Presentation
      岡山
    • Year and Date
      2013-03-21
    • Related Report
      2013 Final Research Report
  • [Presentation] サーモスペルミンによる木部分化の抑制に関わるシロイヌナズナの転写因子SAC51 の分子遺伝学的解析2013

    • Author(s)
      吉本香織・本瀬宏康・高橋卓
    • Organizer
      第54回日本植物生理学会年会
    • Place of Presentation
      岡山
    • Year and Date
      2013-03-21
    • Related Report
      2013 Final Research Report
  • [Presentation] シロイヌナズナのSAC51遺伝子はサーモスペルミンにより翻訳が促進される2013

    • Author(s)
      高橋卓・高野綾香・石塚壮一・石井菜水・懸樋潤一・本瀬宏康
    • Organizer
      第4回日本ポリアミン学会年会
    • Place of Presentation
      松島
    • Year and Date
      2013-01-24
    • Related Report
      2013 Final Research Report
  • [Presentation] SAC51 mRNA はサーモスペルミン及びリボソームタンパク質の変異により安定化する2013

    • Author(s)
      懸樋潤一・河野恵理・本瀬宏康・高橋卓
    • Organizer
      第4回日本ポリアミン学会年会
    • Place of Presentation
      松島
    • Year and Date
      2013-01-24
    • Related Report
      2013 Final Research Report
  • [Presentation] シロイヌナズナの矮性変異acl5 に対する新たなサプレッサー変異株の分子遺伝学的解析2012

    • Author(s)
      福島弘子・河野恵理・坂本智昭・本瀬宏康・倉田哲也・高橋卓
    • Organizer
      第35回日本分子生物学会年会
    • Place of Presentation
      福岡
    • Year and Date
      2012-12-11
    • Related Report
      2013 Final Research Report
  • [Presentation] SAC51 mRNA はサーモスペルミン及びリボソームタンパク質の変異により安定化する2012

    • Author(s)
      懸樋潤一・河野恵理・本瀬宏康・高橋卓
    • Organizer
      日本植物学会第76回大会
    • Place of Presentation
      姫路
    • Year and Date
      2012-09-15
    • Related Report
      2013 Final Research Report
  • [Presentation] 植物界におけるサーモスペルミンの分布2012

    • Author(s)
      高野綾香・懸樋潤一・高橋卓
    • Organizer
      日本植物学会第76回大会
    • Place of Presentation
      姫路
    • Year and Date
      2012-09-15
    • Related Report
      2013 Final Research Report
  • [Presentation] シロイヌナズナSAC51 遺伝子のサーモスペルミンによる翻訳促進2012

    • Author(s)
      石塚壮一・石井菜水・桑城克隆・高橋卓
    • Organizer
      日本植物学会第76回大会
    • Place of Presentation
      姫路
    • Year and Date
      2012-09-15
    • Related Report
      2013 Final Research Report
  • [Presentation] Translational regulation of the SAC51 mRNA by thermospermine2012

    • Author(s)
      A. Takano, S. Ishitsuka, N. Ishii and T. Takahashi
    • Organizer
      24th International Conference on Arabidopsis Research
    • Place of Presentation
      Sydney, Australia
    • Related Report
      2013 Annual Research Report 2013 Final Research Report
  • [Presentation] SAC51 mediates thermospermine-dependent repression of xylem proliferation2012

    • Author(s)
      K. Yoshimoto, W. Tong, H. Fukushima, H. Motose and T. Takahashi
    • Organizer
      24th International Conference on Arabidopsis Research
    • Place of Presentation
      Sydney, Australia
    • Related Report
      2013 Annual Research Report 2013 Final Research Report
  • [Presentation] Functional analysis of thermospermine in the translational control of SAC51, a key regulator of stem growth in Arabidopsis thaliana2011

    • Author(s)
      石塚壮一
    • Organizer
      第34回日本分子生物学会年会
    • Place of Presentation
      パシフィコ横浜
    • Year and Date
      2011-12-15
    • Related Report
      2011 Annual Research Report
  • [Presentation] 植物の茎の伸長制御におけるサーモスペルミンの働き2011

    • Author(s)
      高橋卓
    • Organizer
      第84回日本生化学会大会
    • Place of Presentation
      京都国際会館(招待講演)
    • Year and Date
      2011-09-21
    • Related Report
      2011 Annual Research Report
  • [Presentation] ヒメツリガネゴケにおけるシロイヌナズナACL5類似遺伝子の機能解析2011

    • Author(s)
      懸樋潤一
    • Organizer
      日本植物学会第75回大会
    • Place of Presentation
      東京大学駒場キャンパス
    • Year and Date
      2011-09-19
    • Related Report
      2011 Annual Research Report
  • [Presentation] Analyses of the effect of exogenous polyamines on the stem growth of Arabidopsis thaliana2011

    • Author(s)
      J.-I.Kakehi, et al
    • Organizer
      22nd International Conference on Arabidopsis Research
    • Place of Presentation
      Madison, WI, USA
    • Year and Date
      2011-06-24
    • Related Report
      2011 Annual Research Report
  • [Presentation] シロイヌナズナの表皮細胞分化に必須なホメオボックス遺伝子ATML1とPDF2の発現に関する研究2010

    • Author(s)
      小川枝里子, ほか
    • Organizer
      第33回日本分子生物学会年会
    • Place of Presentation
      神戸
    • Year and Date
      2010-12-08
    • Related Report
      2010 Annual Research Report
  • [Presentation] ケミカルバイオロジーによるシロイヌナズナ維管束制御因子SAC51の発現解析2010

    • Author(s)
      吉本香織, ほか
    • Organizer
      第33回日本分子生物学会年会
    • Place of Presentation
      神戸
    • Year and Date
      2010-12-08
    • Related Report
      2010 Annual Research Report
  • [Presentation] ATHL1 and PDF2 are essential in Arabidopsis embryo development.2010

    • Author(s)
      Ogawa, et al.
    • Organizer
      21th International Conference on Arabidopsis Research
    • Place of Presentation
      Yokohama, Japan
    • Year and Date
      2010-06-08
    • Related Report
      2010 Annual Research Report
  • [Presentation] Identification of thermospermine-responsive genes in Arabidopsis thaliana.2010

    • Author(s)
      Wurina, et al.
    • Organizer
      21th International Conference on Arabidopsis Research
    • Place of Presentation
      Yokohama, Japan
    • Year and Date
      2010-06-08
    • Related Report
      2010 Annual Research Report
  • [Presentation] The SAC51 mRNA is stabilized by thermospermine and mutations of ribosomal proteins

    • Author(s)
      Kakehi, J.
    • Organizer
      23rd International Conference on Arabidopsis Research
    • Place of Presentation
      Vienna, Austria
    • Related Report
      2012 Annual Research Report
  • [Presentation] Isolation of polyamine-resistant mutants of Arabidopsis thaliana

    • Author(s)
      Wurina
    • Organizer
      23rd International Conference on Arabidopsis Research
    • Place of Presentation
      Vienna, Austria
    • Related Report
      2012 Annual Research Report
  • [Presentation] A chemical-biology approach reveals a counteraction of thermospermine to auxin in xylem formation in Arabidopsis thaliana

    • Author(s)
      Yoshimoto, Y.
    • Organizer
      23rd International Conference on Arabidopsis Research
    • Place of Presentation
      Vienna, Austria
    • Related Report
      2012 Annual Research Report
  • [Presentation] シロイヌナズナSAC51遺伝子のサーモスペルミンによる翻訳促進

    • Author(s)
      石塚壮一
    • Organizer
      日本植物学会第76回大会
    • Place of Presentation
      姫路
    • Related Report
      2012 Annual Research Report
  • [Presentation] 植物界におけるサーモスペルミンの分布

    • Author(s)
      高野綾香
    • Organizer
      日本植物学会第76回大会
    • Place of Presentation
      姫路
    • Related Report
      2012 Annual Research Report
  • [Remarks]

    • URL

      http://www.biol.okayama-u.ac.jp/takahashiTaku/T-Takahashi.html

    • Related Report
      2013 Final Research Report

URL: 

Published: 2010-08-23   Modified: 2019-07-29  

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