| Budget Amount *help |
¥17,940,000 (Direct Cost: ¥13,800,000、Indirect Cost: ¥4,140,000)
Fiscal Year 2013: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
Fiscal Year 2012: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
Fiscal Year 2011: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
Fiscal Year 2010: ¥8,580,000 (Direct Cost: ¥6,600,000、Indirect Cost: ¥1,980,000)
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| Research Abstract |
Thermospermine functions in repressing xylem differentiation in plants and its deficiency results in excess proliferation of xylem vessels and severe dwarfism. To know the mode of action of thermospermine, we have isolated suppressor mutants of a thermospermine-requiring mutant of Arabidopsis that restore the dwarf phenotype, and revealed that sac53-d and sac56-d mutants are defective in a gene encoding ribosomal protein RACK1 and L4, respectively. We further found that thermospermine can be functionally replaced with norspermine. A chemical screening experiment revealed that thermospermine and auxin have an opposite function in xylem differentiation.
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