ChloroplastSignalsregulatingplantimmuneresponses

• Project/Area Number: 22370022
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Plant molecular biology/Plant physiology
• Research Institution: Kyoto Prefectural University
• Principal Investigator: SHIINA Takashi 京都府立大学, 大学院・生命環境科学研究科, 教授 (10206039)
• Co-Investigator(Kenkyū-buntansha): YOSHIOKA Hirofumi 名古屋大学, 大学院・生命農学研究科, 准教授 (30240245)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥14,170,000 (Direct Cost: ¥10,900,000、Indirect Cost: ¥3,270,000); Fiscal Year 2012: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2011: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2010: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
• Keywords: 植物分子機能 / 葉緑体 / Ca2+ / サリチル酸 / 感染防御 / 逆行性シグナル / 植物病理学 / 光合成 / CAS / エリシター / WRKY / 一重項酸素 / 感染防御応答 / シロイヌナ / プラスチドシグナル / Ca^<2+>

Research Abstract

Salicylic acid acts as an endogenous signal that triggers local and systemic response in the plant immune system. Both reactive oxygen species (ROS) and nitric oxide (NO) may play important roles in regulation of immune cell signal transductions. It should be noted that chloroplasts are involved in the production of theses immune signals. However, a role for chloroplasts in plant immune system remains largely elusive. This study provides novel insights into chloroplastic control of plant immunity through a thylakoid-localized Ca2+ binding protein CAS.

Research Products

• [Journal Article] Chloroplast envelope localization of EDS5, an essential factor for salicylic acid biosynthesis in Arabidopsis thaliana. (2013)
  • Author(s): Yamasaki K, Motomura Y, Yagi Y, Nomura H, Kikuchi S, Nakai M, Shiina T.
  • Journal Title: Plant Signal Behav. Volume: 18
  • Peer Reviewed
• [Journal Article] Vascular plant one-zinc-finger protein 1/2 transcription factors regulate abiotic and biotic stress responses in Arabidopsis. (2013)
  • Author(s): Nakai Y, Nakahira Y, Sumida H, Takebayashi K, Nagasawa Y, Yamasaki K, Akiyama M, Ohme-Takagi M, Fujiwara S, Shiina T, Mitsuda N, Fukusaki E, Kubo Y, Sato MH.
  • Journal Title: Plant J. Volume: 2013 73 Issue: 5 Pages: 761-75
  • DOI: 10.1111/tpj.12069
  • Peer Reviewed
• [Journal Article] The variable domain of a plant calcium-dependent protein kinase (CDPK) confers subcellular localization and substrate recognition for NADPH oxidase (2013)
  • Author(s): Asai S., Ichikawa T., Kobayashi M., Nomura H., Kamiyoshihara Y., Mori H., Kadota Y., Zipfel C., Jones J. D. G., Yoshioka H.
  • Journal Title: Journal of Biological Chemistry Volume: 288 Issue: 20 Pages: 14332-14340
  • DOI: 10.1074/jbc.m112.448910
  • Peer Reviewed
• [Journal Article] Chloroplast envelope localization of EDS5, an essential factor for salicylic acid biosynthesis in Arabidopsis thaliana. (2013)
  • Author(s): Yamasaki K
  • Journal Title: Plant Signal Behav. Volume: 8
  • Peer Reviewed
• [Journal Article] The variable domain of a plant calcium-dependent protein kinase (CDPK) confers subcellular localization and substrate recognition for NADPH oxidase. (2013)
  • Author(s): Asai S
  • Journal Title: J Biol Chem. Volume: in press
  • Peer Reviewed
• [Journal Article] Eukaryotic-type plastid nucleoid protein pTAC3 is essential for transcription by the bacterial-type plastid RNA polymerase. (2012)
  • Author(s): Yagi Y, Ishizaki Y, Nakahira Y, Tozawa Y, Shiina T.
  • Journal Title: Proc Natl Acad Sci U S A. Volume: 109 Issue: 19 Pages: 7541-6
  • DOI: 10.1073/pnas.1119403109
  • Peer Reviewed
• [Journal Article] Chloroplast-mediated activation of plant immune signalling in Arabidopsis. (2012)
  • Author(s): Nomura H, Komori T, Uemura S, Kanda Y, Shimotani K, Nakai K, Furuichi T, Takebayashi K, Sugimoto T, Sano S, Suwastika IN, Fukusaki E, Yoshioka H, Nakahira Y, Shiina T.
  • Journal Title: Nat Commun.26 Volume: 3 Issue: 1 Pages: 926-926
  • DOI: 10.1038/ncomms1926
  • Peer Reviewed
• [Journal Article] Defense response of a pepper cultivar cv. Sy-2 is induced at temperatures below 24°C. (2012)
  • Author(s): Koeda S, Hosokawa M, Kang BC, Tanaka C, Choi D, Sano S, Shiina T, Doi M, Yazawa S.
  • Journal Title: J Plant Res. Volume: 125 Pages: 137-45
  • URL: http://link.springer.com/article/10.1007%2Fs10265-011-0414-1
  • Peer Reviewed
• [Journal Article] Functional characterization of ObgC in ribosome biogenesis during chloroplast development. (2012)
  • Author(s): Bang WY, Chen J, Jeong IS, Kim SW, Kim CW, Jung HS, Lee KH, Kweon HS, Yoko I, Shiina T, Bahk JD.
  • Journal Title: Plant J. Volume: 71 Issue: 1 Pages: 122-34
  • DOI: 10.1111/j.1365-313x.2012.04976.x
  • Peer Reviewed
• [Journal Article] Evolutionary aspects of plastid proteins involved in transcription: The transcription of a tiny genome is mediated by a complicated machinery. (2012)
  • Author(s): Yagi Y, Shiina T.
  • Journal Title: Transcription. Volume: 3 Pages: 1-5
  • URL: http://www.landesbioscience.com/journals/transcription/article/21810/?nocache=1659960848
  • Peer Reviewed
• [Journal Article] StCDPK5 confers resistance to late blight pathogen but increases susceptibility to early blight pathogen in potato via reactive oxygen species burst. (2012)
  • Author(s): Nomura H, Kuchimura K, Mori H, Doke N, Yoshioka H.
  • Journal Title: New Phytol. Volume: 196 Issue: 1 Pages: 223-37
  • DOI: 10.1111/j.1469-8137.2012.04226.x
  • Peer Reviewed
• [Journal Article] Evolutionary aspects of plastid proteins involved in transcription: The transcription of a tiny genome is mediated by a complicated machinery. (2012)
  • Author(s): Yagi Y
  • Journal Title: Transcription Volume: 3 Pages: 1-5
  • Peer Reviewed
• [Journal Article] Use of ultra-performance liquid chromatography/time-of-flight mass spectrometry with nozzle-skimmer fragmentation for comprehensive quantitative analysis of secondary metabolites in Arabidopsis thaliana. (2011)
  • Author(s): Sugimoto T, Bamba T, Izumi Y, Nomura H, Shiina T, Fukusaki E.
  • Journal Title: J Sep Sci. Volume: 34 Issue: 24 Pages: 3587-96
  • DOI: 10.1002/jssc.201100552
  • Peer Reviewed
• [Journal Article] Nuclear/nucleolar GTPase 2 proteins as a subfamily of YlqF/YawG GTPases function in pre-60S ribosomal subunit maturation of mono- and dicotyledonous plants. (2011)
  • Author(s): Im CH, Hwang SM, Son YS, Heo JB, Bang WY, Suwastika IN, Shiina T, Bahk JD.
  • Journal Title: J Biol Chem. Volume: 286 Issue: 10 Pages: 8620-3
  • DOI: 10.1074/jbc.m110.200816
  • Peer Reviewed
• [Journal Article] Nuclear/nucleolar GTPase 2 proteins as a subfamily of YlqF/YawG GTPases function in pre-60S ribosomal subunit maturation of mono- and dicotyledonous plants. (2011)
  • Author(s): Im CH., Hwang SM., Son YS., Heo JB., Bang WY., Suwastika IN., Shiina T., Bahk JD
  • Journal Title: J.Biol.Chem. Volume: 286 Pages: 8620-8632
  • Peer Reviewed
• [Journal Article] Defense response of a pepper cultivar cv.Sy-2 is induced at temperatures below 24℃. (2011)
  • Author(s): Koeda S., Hosokawa M., Kang BC., Tanaka C., Choi D., Sano S., Shiina T., Doi M., Yazawa S.
  • Journal Title: J Plant Res. Volume: (in press)
  • Peer Reviewed
• [Journal Article] Phosphorylation of the Nicotiana benthamiana WRKY8 Transcription Factor by MAPK Functions in the Defense Response. (2011)
  • Author(s): Ishihama N., Yamada R., Yoshioka M., Katou S., Yoshioka H.
  • Journal Title: Plant Cell Volume: 23 Pages: 1153-1170
  • Peer Reviewed
• [Journal Article] Selective Excitation of Photosystems in Chloroplasts inside Plant Leaves Observed by Near-Infrared Laser-Based Fluorescence Spectral Microscopy (2010)
  • Author(s): Makoto Hasegawa, Takashi Shiina, Masahide Terazima and *Shigeichi Kumazaki
  • Journal Title: Plant Cell Physiol. Volume: 51 Issue: 2 Pages: 225-238
  • DOI: 10.1093/pcp/pcp182
  • NAID: 10027346696
  • Peer Reviewed
• [Journal Article] Selective excitation of photosystems in chloroplasts inside plantleaves observed by near-infrared laser-based fluorescence spectral microscopy. (2010)
  • Author(s): Hasegawa, M., Shiina, T., Terajima, M., Kumasaki S.
  • Journal Title: Plant Cell Physiol. Volume: 51 Pages: 225-238
  • NAID: 10027346696
  • Peer Reviewed
• [Journal Article] A key enzyme for flavin synthesis is required for nitric oxide and reactive oxygen species production in disease resistance. (2010)
  • Author(s): Asai S, Mase K., Yoshioka H.
  • Journal Title: Plant J Volume: 62 Pages: 911-924
  • Peer Reviewed
• [Journal Article] Role of nitric oxide and reactive oxide species in disease resistance to necrotrophic pathogens. (2010)
  • Author(s): Asai S, Mase K., Yoshioka H.
  • Journal Title: Plant Signal Behav. Volume: 5 Pages: 872-874
  • Peer Reviewed
• [Journal Article] Regulatory mechanisms of nitric oxide and reactive oxygen species generation and their role in plant immunity. (2010)
  • Author(s): Yoshioka H., Mase K., Yoshioka M., Kobayashi M., Asai S.
  • Journal Title: Nitric Oxide Volume: (in press)
  • Peer Reviewed
• [Presentation] Chloroplast Ca2+ and Plant Immunity (2013)
  • Author(s): Takashi Shiina
  • Organizer: 3rd International Symposium on Chloroplast Genomics and Genetic Engineering
• [Presentation] 植物の細胞内シグナル伝達系と共生オルガネラ (2013)
  • Author(s): 椎名隆
  • Organizer: 第54回植物生理学会(シンポジウム)
• [Presentation] (2013)
  • Author(s): Takashi Shiina
  • Organizer: 29th IPSR International Symposium and 5th Symposium on Plant Sciences
• [Presentation] 植物の細胞内シグナル伝達系と共生オルガネラ (2013)
  • Author(s): 椎名隆
  • Organizer: 第54回植物生理学会
  • Place of Presentation: 岡山市
  • Invited
• [Presentation] ミトコンドリアのCa2+センサータンパク質候補AtMICU1の機能解析 (2013)
  • Author(s): 浅倉千洋
  • Organizer: 第54回植物生理学会
  • Place of Presentation: 岡山市
• [Presentation] 葉緑体CASタンパク質による防御応答遺伝子の発現制御 (2013)
  • Author(s): 下谷 紘司
  • Organizer: 第54回植物生理学会
  • Place of Presentation: 岡山市
• [Presentation] ミトコンドリア機械受容チャネルMSL1の機能解析 (2013)
  • Author(s): 𦫿原佐紀
  • Organizer: 第54回植物生理学会
  • Place of Presentation: 岡山市
• [Presentation] 葉緑体ストレス応答プロモータpsbD KRPの構造とストレス応答性の進化 (2013)
  • Author(s): 木寅 翔太
  • Organizer: 第54回植物生理学会
  • Place of Presentation: 岡山市
• [Presentation] Crosstalk between Plant Immunity and Chloroplasts (2013)
  • Author(s): 椎名隆
  • Organizer: 29th IPSR International Symposium and 5th Symposium on Plant Sciences
  • Place of Presentation: 倉敷市
  • Invited
• [Presentation] Chloroplast Ca2+ and Plant Immunity (2013)
  • Author(s): 椎名隆
  • Organizer: 3rd International Symposium on Chloroplast Genomics and Genetic Engineering
  • Place of Presentation: アメリカ NewBrunswick市
  • Invited
• [Presentation] Analysis of the light dependent association of chloroplast RNA polymerase complex with chloroplast DNA in wheat (2012)
  • Author(s): Motomura, Y., Yagi, Y., Nakahira, Y., Shiina, T
  • Organizer: 日本植物生理学会第54回大会
  • Place of Presentation: 京都
  • Year and Date: 2012-03-17
• [Presentation] Light-dependent expression of PAMP-induced defense genes (2012)
  • Author(s): Nakai, K., Kin, R., Nakahira, Y., Shiina, T.
  • Organizer: 日本植物生理学会第54回大会
  • Place of Presentation: 京都
  • Year and Date: 2012-03-17
• [Presentation] ウルシノキのトランスクリフ・トーム解析 (2012)
  • Author(s): 石崎陽子, 岡本嘉明, 佐藤貴彦, 大藪泰, 椎名隆
  • Organizer: 日本植物生理学会第54回大会
  • Place of Presentation: 京都
  • Year and Date: 2012-03-17
• [Presentation] ウルシノキのトランス クリプトーム解析 (2012)
  • Author(s): 石崎陽子
  • Organizer: 第76回植物学会
  • Place of Presentation: 姫路市
• [Presentation] 光による防御応答遺伝 子の発現制御 (2012)
  • Author(s): 中井 香奈
  • Organizer: 第76回植物学会
  • Place of Presentation: 姫路市
• [Presentation] 葉緑体CAS タンパク質 による防御応答関連転 写因子群の発現制御 (2012)
  • Author(s): 下谷 紘司
  • Organizer: 第76回植物学会
  • Place of Presentation: 姫路市
• [Presentation] 葉緑体psbD 光応答プロモーターの光応答及びス トレス応答機能について (2012)
  • Author(s): 木寅 翔太
  • Organizer: 第76回植物学会
  • Place of Presentation: 姫路市
• [Presentation] Light-dependent Expression of PAMP-induced Defense Genes (2012)
  • Author(s): 中井香奈
  • Organizer: 10th International Plant Molecular Biology Congress
  • Place of Presentation: 済州島
• [Presentation] Transcription Factors Under the Control of Chloroplast-derived Immune Signalling Arabidopsis thaliana (2012)
  • Author(s): 下谷紘司
  • Organizer: 10th International Plant Molecular Biology Congress
  • Place of Presentation: 済州島
• [Presentation] Evolutionary Analysis of Plastid Psbd Light-responsive Promoters and Their Stress Response in Land Plants (2012)
  • Author(s): 木寅翔太
  • Organizer: 10th International Plant Molecular Biology Congress
  • Place of Presentation: 済州島
• [Presentation] サリチル酸前駆体トランスポーターEDS5は葉緑体包膜に存在する (2011)
  • Author(s): 本村幸也, 山崎加奈子, 佐野智, 中平洋一, 椎名隆
  • Organizer: 日本植物学会第75回大会
  • Place of Presentation: 東京
  • Year and Date: 2011-09-19
• [Presentation] Analysis Characterization of mitochondrial Ca^<2+> sensor protein AtMICU1 function in Arabidopsis (2011)
  • Author(s): Yamaguchi, S., Yagi, Y., Shiina, T.
  • Organizer: 日本植物学会第75回大会
  • Place of Presentation: 東京
  • Year and Date: 2011-09-19
• [Presentation] Identification and Characterization of defense related transcription factors under the control of the thylakoid protein CAS in Arabidopsis (2011)
  • Author(s): Shimotani, K., m Kanda, Y., Nakahira, Y., Shiina, T.
  • Organizer: 日本植物学会第75回大会
  • Place of Presentation: 東京
  • Year and Date: 2011-09-19
• [Presentation] 葉緑体Ca2+シグナリングと植物のストレス・感染防御応答 (2011)
  • Author(s): 椎名隆, 野村裕也
  • Organizer: 日本植物学会第75回大会
  • Place of Presentation: 東京(招待講演)
  • Year and Date: 2011-09-17
• [Presentation] 葉緑体による感染防御遺伝子の制御機構 (2011)
  • Author(s): 椎名隆, 神田ゆい, 植村周平, 佐野智, 中平洋一, 野村裕也
  • Organizer: 日本植物学会第75回大会
  • Place of Presentation: 東京
  • Year and Date: 2011-09-17
• [Presentation] トウガラシ'Sy-2'の防御応答は生育温度が24℃を下回ると誘導される (2011)
  • Author(s): 小枝壮太, 他9名
  • Organizer: 日本植物学会第75回大会
  • Place of Presentation: 東京
  • Year and Date: 2011-09-17
• [Presentation] I植物防御応答における葉緑体の役割 (2011)
  • Author(s): 野村裕也、椎名隆
  • Organizer: オルガネラワークショップ
  • Place of Presentation: 東京(招待講演)
  • Year and Date: 2011-09-17
• [Presentation] 野村裕也葉緑体Ca2+シグナリングと植物のストレス・感染防御応答 (2011)
  • Author(s): 椎名隆
  • Organizer: 日本植物学会第75回大会(シンポジウム)
• [Presentation] CAS is a chloroplast protein regulating biosynthesis of salicylic acid and plant immune signaling (2011)
  • Author(s): H.Nomura, 他9名
  • Organizer: International Botanical Congress 2011
  • Place of Presentation: メルボルン市
• [Presentation] The dynamics of nucleolar small G protein AtNog1-1 in plant nucleus is cell cycle and cell development dependent (2011)
  • Author(s): Suwastika, IN, 他6名
  • Organizer: International Botanical Congress 2011
  • Place of Presentation: メルボルン市
• [Presentation] 植物の感染防御応答におけるサリチル酸誘導と葉緑体チラコイド膜タンパク質CAS (2011)
  • Author(s): 椎名隆, 中平洋一, 他
  • Organizer: 第52回植物生理学会年会
  • Place of Presentation: 仙台市
• [Presentation] CHIP法を用いたコムギ葉緑体RNAポリメラーゼPEP複合体の動態解析 (2011)
  • Author(s): 八木祐介, 中平洋一, 椎名隆
  • Organizer: 第52回植物生理学会年会
  • Place of Presentation: 仙台市
• [Presentation] シロイヌナズナNAC関連転写院試VOZは非生物学的ストレス応答の制御に関与している (2011)
  • Author(s): 中井勇介, 中平洋一, 椎名隆, 他
  • Organizer: 第52回植物生理学会年会
  • Place of Presentation: 仙台市
• [Presentation] 植物免疫におけるラジカルバーストに関わるタンパク質の機能解析 (2011)
  • Author(s): 野村裕也, 椎名隆, 吉岡博文, 他
  • Organizer: 第52回植物生理学会年会
  • Place of Presentation: 仙台市
• [Presentation] Cell cycle dependent dynamics of nucleolar small G protein AtNog1-1 in plant cells. (2011)
  • Author(s): Suwastika IN, Shiina T, et al.
  • Organizer: 第52回植物生理学会年会
  • Place of Presentation: 仙台市
• [Presentation] 野村裕也植物免疫応答におけるCa2+シグナルとCAS の役割 (2010)
  • Author(s): 椎名隆
  • Organizer: 日本植物学会第74回大会(シンポジウム)
• [Presentation] 植物免疫応答における葉緑体Ca2+シグナルとCASの役割 (2010)
  • Author(s): 野村裕也, 椎名隆
  • Organizer: 第74回日本植物学会シンポジウム
  • Place of Presentation: 春日井市
• [Presentation] Sub nuclear dynamics of nonribosaomal-small G protein AtNOG1 in Arabidopsis. (2010)
  • Author(s): Suwastika IN., Takeyasu K., Shiina T.
  • Organizer: Asian Congress on Trabscription 11
  • Place of Presentation: 沖縄
• [Presentation] Localization analysis of ssDNA/RNA binding protein, AtTranslin. (2010)
  • Author(s): Yagi Y., Motomura Y., Shiina T.
  • Organizer: Asian Congress on Trabscription 11
  • Place of Presentation: 沖縄
• [Presentation] Evolution of a stress-responsive promoter in chloroplast. (2010)
  • Author(s): Shimmura S., Nozoe M., Kin S., Nakahira Y., Shiina T.
  • Organizer: Asian Congress on Trabscription 11
  • Place of Presentation: 沖縄
• [Book] 有機農業と遺伝子組換え食品-明日の食卓- (2011)
  • Author(s): 椎名隆・石崎陽子監訳
  • Total Pages: 288
  • Publisher: 丸善出版