The study for the mechanism of steroid hormone-dependent sex determination in amphibians
| Project/Area Number |
22370027
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Morphology/Structure
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| Research Institution | Waseda University |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
松田 洋一 (70165835)
長谷部 孝 (70329027)
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| Project Period (FY) |
2010-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥18,460,000 (Direct Cost: ¥14,200,000、Indirect Cost: ¥4,260,000)
Fiscal Year 2013: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Fiscal Year 2012: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2011: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2010: ¥7,150,000 (Direct Cost: ¥5,500,000、Indirect Cost: ¥1,650,000)
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| Keywords | 組織構築 / アンドロゲン / アンドロゲン受容体 / トランスゲニックカエル / 性転換 / ツチガエル / 性染色体 / 性決定 / 免疫染色 / 性決定関連遺伝子 / 両生類 / 性分化 / 歯伝子機能解析 / ステロイドホルモン / トランスゲネシス / 遺伝子機能解析 |
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| Research Abstract |
Sex of an amphibian species Rana rugosa can be changed from female to male or in the opposite direction by treatment of sex steroid hormones. To induce the sex-reversal, androgens must bind its receptor. The androgen receptor (AR) gene is localized to the Z and W chromosome. The AR gene on the Z chromosome (Z-AR) is expressed normally, but W-AR is barely expressed, suggesting that this gene is probably involved in male sex determination in R. rugosa frogs. To prove this hypothesis, we produced transgenic frogs carrying an exogenous Z-AR. AR-transgenic female (ZW) frogs formed masculinized gonad or 'ovotestis'. Testosterone, supplied to the rearing water, completed the female-to-male sex-reversal in the AR-transgenic ZW frogs. This is the first report showing that the AR gene plays a very important role in male sex-determination in a vertebrate species.
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Report
(5 results)
Research Products
(28 results)
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[Journal Article] Parathyroid hormone 1 (1-34) acts on the scales and involves calcium metabolism in goldfish2011
Author(s)
N Suzuki, J.A. Danks, Y. Maruyama, M. Ikegame, Y. Sasayama, A. Hattori, M. Nakamura, M.J. Tabata, T. Yamamoto, R. Furuya, K. Saijoh, H. Mishima, A.K. Srivastav, Y. Furusawa, T. Kondo, Y. Tabuchi, I. Takasaki, V.S. Chowdhury, K. Hayakawa, T.J. Martin
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Journal Title
Bone
Volume: 48
Pages: 1186-1193
NAID
Related Report
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