| Project/Area Number |
22370028
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (B)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Animal physiology/Animal behavior
|
|---|
| Research Institution | The University of Tokyo |
|---|
Principal Investigator |
SHINGYOJI Chikako 東京大学, 理学(系)研究科(研究院), 准教授 (80125997)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
KITAJIMA Ken 名古屋大学, 生物機能開発利用研究センター, 教授 (80192558)
|
|---|
| Co-Investigator(Renkei-kenkyūsha) |
SHIBA Kogiku 筑波大学, 大学院生命環境科学研究科, 助教 (70533561)
|
|---|
| Project Period (FY) |
2010-04-01 – 2013-03-31
|
| Project Status |
Completed (Fiscal Year 2013)
|
| Budget Amount *help |
¥18,330,000 (Direct Cost: ¥14,100,000、Indirect Cost: ¥4,230,000)
Fiscal Year 2012: ¥6,630,000 (Direct Cost: ¥5,100,000、Indirect Cost: ¥1,530,000)
Fiscal Year 2011: ¥6,630,000 (Direct Cost: ¥5,100,000、Indirect Cost: ¥1,530,000)
Fiscal Year 2010: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
|
|---|
| Keywords | カルシウム / 生体分子 / 生理学 / 細胞・組織 / 糖鎖 / 精子 / 鞭毛運動 / 機械刺激 / 膜タンパク質 / ラフト / 細胞 / 遺伝子 |
|---|
| Research Abstract |
Flagellar movement of the sea urchin sperm is regulated by intracellular Ca2+. To study the mechanism of Ca2+ dynamics underlying flagellar movement, we analyzed the mechanosensory behavioural responses. In the presence of 10 mM Ca2+, the sperm swim in circular paths (C). When a mechanical stimulus was applied to the head, the sperm showed a series of flagellar responses, consisting of a stoppage of beating (Q) and a recovery of swimming in a straight path (S), followed by swimming in a circular path again; as the result the sperm avoided the obstacle. Ca2+-imaging showed that the intracellular Ca2+ was high in the Q and the early stage of S, and gradually decreased after that. The Ca2+ influx was induced by Ca2+ channels and the Ca2+ efflux was induced by a flagellasialin-related Ca2+-efflux system, PMCA and the NCKX. Separate experiments revealed that the initiation of flagellar movement of the sperm was regulated by the intracellular Ca2+ through the Ca2+ stores.
|
