Calcium dynamics in the regulation of flagellar movement of swimming spermatozoa
| Project/Area Number |
22370028
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Animal physiology/Animal behavior
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| Research Institution | The University of Tokyo |
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Principal Investigator |
SHINGYOJI Chikako 東京大学, 理学(系)研究科(研究院), 准教授 (80125997)
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| Co-Investigator(Kenkyū-buntansha) |
KITAJIMA Ken 名古屋大学, 生物機能開発利用研究センター, 教授 (80192558)
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| Co-Investigator(Renkei-kenkyūsha) |
SHIBA Kogiku 筑波大学, 大学院生命環境科学研究科, 助教 (70533561)
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| Project Period (FY) |
2010-04-01 – 2013-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥18,330,000 (Direct Cost: ¥14,100,000、Indirect Cost: ¥4,230,000)
Fiscal Year 2012: ¥6,630,000 (Direct Cost: ¥5,100,000、Indirect Cost: ¥1,530,000)
Fiscal Year 2011: ¥6,630,000 (Direct Cost: ¥5,100,000、Indirect Cost: ¥1,530,000)
Fiscal Year 2010: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
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| Keywords | カルシウム / 生体分子 / 生理学 / 細胞・組織 / 糖鎖 / 精子 / 鞭毛運動 / 機械刺激 / 膜タンパク質 / ラフト / 細胞 / 遺伝子 |
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| Research Abstract |
Flagellar movement of the sea urchin sperm is regulated by intracellular Ca2+. To study the mechanism of Ca2+ dynamics underlying flagellar movement, we analyzed the mechanosensory behavioural responses. In the presence of 10 mM Ca2+, the sperm swim in circular paths (C). When a mechanical stimulus was applied to the head, the sperm showed a series of flagellar responses, consisting of a stoppage of beating (Q) and a recovery of swimming in a straight path (S), followed by swimming in a circular path again; as the result the sperm avoided the obstacle. Ca2+-imaging showed that the intracellular Ca2+ was high in the Q and the early stage of S, and gradually decreased after that. The Ca2+ influx was induced by Ca2+ channels and the Ca2+ efflux was induced by a flagellasialin-related Ca2+-efflux system, PMCA and the NCKX. Separate experiments revealed that the initiation of flagellar movement of the sperm was regulated by the intracellular Ca2+ through the Ca2+ stores.
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Report
(4 results)
Research Products
(28 results)
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[Journal Article] Mechanism regulating Ca2+-dependent mechanosensory behavi-our in sea urchin spermatozoa2011
Author(s)
Kambara, Y., Shiba, K., Yoshida, M., Sato, C., Kitajima, Ken., Shingyoji, C.
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Journal Title
Cell Structure and Function
Volume: 36
Pages: 69-82
NAID
Related Report
Peer Reviewed
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