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Mass Spectrometry of Metalloproteins and High-Molecular-Weight Protein Complexes

Research Project

Project/Area Number 22370040
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Structural biochemistry
Research InstitutionOsaka University

Principal Investigator

TAKAO Toshifumi  大阪大学, 蛋白質研究所, 教授 (10197048)

Co-Investigator(Renkei-kenkyūsha) OKUMURA Nobuaki  大阪大学, たんぱく質研究所, 准教授 (20224173)
Project Period (FY) 2010 – 2012
Project Status Completed (Fiscal Year 2012)
Budget Amount *help
¥18,200,000 (Direct Cost: ¥14,000,000、Indirect Cost: ¥4,200,000)
Fiscal Year 2012: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000)
Fiscal Year 2011: ¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000)
Fiscal Year 2010: ¥6,110,000 (Direct Cost: ¥4,700,000、Indirect Cost: ¥1,410,000)
Keywords蛋白質複合体 / 金属蛋白質 / エレクトロスプレーイオン化質量分析 / ネイティブ状態 / 金属イオン / 化学量論 / タンパク質 / エレクトロスプレーイオン化 / 脱アミド化 / 異性化 / 銅イオン / β2-ミクログロブリン / 質量分析 / 酵素 / 生体分子 / 分析科学
Research Abstract

A method for high-efficient ion transmission of high-m/z molecular ions has been developed and embedded in a conventional electrospray ionization mass spectrometer. The method allows for measurement of large molecular complexes such as a protein complex, metalloprotein, etc. in native states, which is useful for revealingstoichiometry of molecules that form a complex or metal ions that are coordinated in a metalloprotein.

Report

(4 results)
  • 2012 Annual Research Report   Final Research Report ( PDF )
  • 2011 Annual Research Report
  • 2010 Annual Research Report
  • Research Products

    (25 results)

All 2013 2012 2011 2010

All Journal Article (12 results) (of which Peer Reviewed: 12 results) Presentation (11 results) Book (2 results)

  • [Journal Article] Quantitative Analysis of Deamidation and Isomerization in β2-Microglobulin by 18O Labeling2012

    • Author(s)
      Fukuda M, Takao T
    • Journal Title

      Anal. Chem

      Volume: 84 Pages: 10388-10394

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Association of aureolic acid antibiotic, chromomycin A3 with Cu(2+) and its negative effect upon DNA binding property of the antibiotic2012

    • Author(s)
      Lahiri S, Takao T, Devi PG, Ghosh S, Ghosh A, Dasgupta A, Dasgupta D
    • Journal Title

      Biometals

      Volume: 25 Pages: 435-450

    • Related Report
      2012 Final Research Report 2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Significance of AlanineAminopeptidase N (APN) in Bile in the Diagnosis of Acute Cellular Rejection After Liver Transplantation2012

    • Author(s)
      Kim C, Aono S, Marubashi S, Wada H, Kobayashi S, Eguchi H, Takeda Y, Tanemura M, Okumura N, Takao T, Doki Y, Mori M, Nagano H.
    • Journal Title

      J Surg Res

      Volume: 175 Pages: 138-148

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Quantitative Analysis of Deamidation and Isomerization in β2-Microglobulin by 18O Labeling2012

    • Author(s)
      Masafumi Fukuda
    • Journal Title

      Analytical Chemistry

      Volume: 84 Issue: 23 Pages: 10388-10394

    • DOI

      10.1021/ac302603b

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Significance of Alanine Aminopeptidase N (APN) in Bile in the Diagnosis of Acute Cellular Rejection After Liver IVansplantation2012

    • Author(s)
      Kim C, et al
    • Journal Title

      J.Surg.Res.

      Volume: 175 Pages: 138-148

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Charge state-selective separation of peptides by reversible modification of amino groups and strong cation-exchange chromatography: evaluation in proteomic studies using peptide-centric database searches.2011

    • Author(s)
      Betancourt LH, Sanchez A, Perez Y, Fernandez de Cossio J, Gil J, Toledo P, Iguchi S, Aimoto S, Gonzalez LJ, Padron G, Takao T, Besada V.
    • Journal Title

      J. Proteomics.

      Volume: 74 Pages: 2210-2213

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] A screen for potential ferredoxin electron transfer partners uncovers new, redox dependent interactions2011

    • Author(s)
      Hanke GT, Satomi Y, Shinmura K, Takao T, Hase T.
    • Journal Title

      Biochim Biophys Acta.

      Volume: 1814 Pages: 366-374

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Charge state-selective separation of peptides by reversible modification of amino groups and strong cation-exchange chromatography : evaluation in proteomic studies using peptide-centric database searches2011

    • Author(s)
      Betancourt LH, et al
    • Journal Title

      J.Proteomics.

      Volume: 74 Pages: 2210-2213

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A screen for potential ferredoxin electron transfer partners uncovers new, redox dependent interactions.2011

    • Author(s)
      Hanke GT, et al.
    • Journal Title

      Biochim Biophys Acta

      Volume: 1814 Pages: 366-374

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Expression of C4.4A at the invasive front is a novel prognostic marker for disease recurrence of colorectal cancer2010

    • Author(s)
      Konishi K, Yamamoto H, Mimori K, Takemasa I, Mizushima T, Ikeda M, Sekimoto, M, Matsuura N, Takao T, Doki Y, Mori M.
    • Journal Title

      Cancer Sci.

      Volume: 101(10) Pages: 2269-2277

    • NAID

      10027832873

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Expression of C4.4A at the invasive front is a novel prognostic marker for disease recurrence of colorectal cancer.2010

    • Author(s)
      Konishi K, et al.
    • Journal Title

      Cancer Sci.

      Volume: 101 Pages: 2269-2277

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Distribution of neuroendocrine regulatory peptide-1 and -2, and proteolytic processing of their precursor VGF protein in the rat.2010

    • Author(s)
      Mishiro-Sato E, et al.
    • Journal Title

      J Neurochem.

      Volume: 114 Pages: 1097-1106

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Presentation] Quantitative analysis of deamidation and isomerization in β2-microglobulin by 18O labeling2013

    • Author(s)
      ukuda M, Takao T
    • Organizer
      The 8th Annual Meeting of Nine Life Science InstitutesAffiliated with the National University in Japan
    • Place of Presentation
      Kyoto University, Kyoto
    • Related Report
      2012 Final Research Report
  • [Presentation] Quantitative analysis of deamidation and isomerization in β2-microglobulin by 18O labeling2013

    • Author(s)
      Masafumi Fukuda
    • Organizer
      The 8th Annual Meeting of Nine Life Science Institutes Affiliated with the National University in Japan
    • Place of Presentation
      Kyoto University, Kyoto
    • Related Report
      2012 Annual Research Report
  • [Presentation] Mass Spectrometric Strategies for the Analysis of Posttranslational Modifications2012

    • Author(s)
      Takao T
    • Organizer
      IPR International Symposium 2012
    • Place of Presentation
      Senri-Hankyu Hotel Suita
    • Year and Date
      2012-11-22
    • Related Report
      2012 Final Research Report
  • [Presentation] プロテオミクスによるバイオマーカー探索2012

    • Author(s)
      高尾敏文
    • Organizer
      生物分子システムに基づく創薬科学フロンティア研究成果発表会y
    • Place of Presentation
      京都薬科大学(招待講演)
    • Year and Date
      2012-02-18
    • Related Report
      2011 Annual Research Report
  • [Presentation] Mass Spectrometry for Analysis of Posttranslational Modifications2012

    • Author(s)
      Takao T
    • Organizer
      Recent Advances in Chemical & Physical Biology Organized by Saha Institute ofNuclear Physics & Mechanobiology Institute, National University of Singapore
    • Place of Presentation
      Kolkata,India.
    • Related Report
      2012 Final Research Report
  • [Presentation] プロテオミクスによるバイオマーカー探索2012

    • Author(s)
      高尾敏文
    • Organizer
      平成23 年度文部科学省私立大学戦略的基盤研究形成事業, 「生物分子システムに基づく創薬科学フロンティア研究」
    • Place of Presentation
      京都薬科大学、京都
    • Related Report
      2012 Final Research Report
  • [Presentation] Mass Spectrometric Strategies for the Analysis of Posttranslational Modifications2012

    • Author(s)
      Toshifumi Takao
    • Organizer
      IPR International Symposium 2012
    • Place of Presentation
      Senri-Hankyu Hotel, Suita
    • Related Report
      2012 Annual Research Report
  • [Presentation] Mass Spectrometry for Analysis of Post-translational Modifications2012

    • Author(s)
      Takao T
    • Organizer
      Recent Advances in Chemical and Physical Biology
    • Place of Presentation
      Saha Institute of Nuclear Physics, India(招待講演)
    • Related Report
      2011 Annual Research Report
  • [Presentation] Analysis of Dimer Formation of Carnosine Dipeptidase 2 (CNDP2) by ESI-TOF/MS2011

    • Author(s)
      Okumura N, Tamura J, Takao T
    • Organizer
      The 59thAnnual Conference on Mass Spectrometry
    • Place of Presentation
      Hotel Hankyu Expo Park, Suita
    • Related Report
      2012 Final Research Report
  • [Presentation] Mass spectrometry for analysis of posttranslational modifications2010

    • Author(s)
      Takao T
    • Organizer
      The 1st International Symposium on Recent Advance in Medical Proteomics Organized by Yokohama City University
    • Place of Presentation
      Yokohama.
    • Year and Date
      2010-09-16
    • Related Report
      2012 Final Research Report
  • [Presentation] Mass Spectrometry for Analysis of Posttranslational Modifications2010

    • Author(s)
      Toshifumi Takao
    • Organizer
      The 1st International Symposium on Recent Advance in Medical Proteomics
    • Place of Presentation
      Yokohama
    • Year and Date
      2010-09-16
    • Related Report
      2010 Annual Research Report
  • [Book] "新規タンパク質の発見法"日本医事新報2010

    • Author(s)
      高尾敏文
    • Total Pages
      87
    • Related Report
      2012 Final Research Report
  • [Book] "疾患・バイオマーカー探索"生化学2010

    • Author(s)
      高尾敏文
    • Related Report
      2012 Final Research Report

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Published: 2010-08-23   Modified: 2019-07-29  

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