Elucidation of nuclear export machinery of tRNA by crystal structural analysis of exportin/tRNA complex
| Project/Area Number |
22370041
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Structural biochemistry
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| Research Institution | Osaka University |
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Principal Investigator |
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| Research Collaborator |
YAMAZAWA Ryuji 大阪大学, 蛋白質研究所, 博士研究員
KOBAYASHI Takeshi 大阪大学, 大学院・理学研究科, 博士前期課程1 年
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| Project Period (FY) |
2010 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥18,460,000 (Direct Cost: ¥14,200,000、Indirect Cost: ¥4,260,000)
Fiscal Year 2012: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000)
Fiscal Year 2011: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000)
Fiscal Year 2010: ¥6,630,000 (Direct Cost: ¥5,100,000、Indirect Cost: ¥1,530,000)
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| Keywords | 繊結晶解析 / 核-細胞質間物質輸送 / 構造生物学 / 核外輸送因子 / tRNA / X線結晶構造解析 |
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| Research Abstract |
In eukaryotic cells, the nucleus, where transcription occurs, and thecytoplasm, where translation takes place, are separated by the nuclear envelope. Theregulation of nucleo-cytoplasmic transport of proteins and RNAs is essential for biologicalactivity. Here, we solve crystal structures of a nuclear export factor and export factorcomplex for non-coding RNA export from the nucleus and the crystal structures reveal RNArecognition mechanism of the nuclear export factor transporting various non cordingRNAs.
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Report
(4 results)
Research Products
(36 results)
