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Elucidation of nuclear export machinery of tRNA by crystal structural analysis of exportin/tRNA complex

Research Project

Project/Area Number 22370041
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Structural biochemistry
Research InstitutionOsaka University

Principal Investigator

YAMASHITA Eiki  大阪大学, 蛋白質研究所, 助教 (00294132)

Research Collaborator YAMAZAWA Ryuji  大阪大学, 蛋白質研究所, 博士研究員
KOBAYASHI Takeshi  大阪大学, 大学院・理学研究科, 博士前期課程1 年
Project Period (FY) 2010 – 2012
Project Status Completed (Fiscal Year 2012)
Budget Amount *help
¥18,460,000 (Direct Cost: ¥14,200,000、Indirect Cost: ¥4,260,000)
Fiscal Year 2012: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000)
Fiscal Year 2011: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000)
Fiscal Year 2010: ¥6,630,000 (Direct Cost: ¥5,100,000、Indirect Cost: ¥1,530,000)
Keywords繊結晶解析 / 核-細胞質間物質輸送 / 構造生物学 / 核外輸送因子 / tRNA / X線結晶構造解析
Research Abstract

In eukaryotic cells, the nucleus, where transcription occurs, and thecytoplasm, where translation takes place, are separated by the nuclear envelope. Theregulation of nucleo-cytoplasmic transport of proteins and RNAs is essential for biologicalactivity. Here, we solve crystal structures of a nuclear export factor and export factorcomplex for non-coding RNA export from the nucleus and the crystal structures reveal RNArecognition mechanism of the nuclear export factor transporting various non cordingRNAs.

Report

(4 results)
  • 2012 Annual Research Report   Final Research Report ( PDF )
  • 2011 Annual Research Report
  • 2010 Annual Research Report
  • Research Products

    (36 results)

All 2013 2012 2011 2010 Other

All Journal Article (20 results) (of which Peer Reviewed: 20 results) Presentation (14 results) Book (1 results) Remarks (1 results)

  • [Journal Article] Cramer W. A., Quinone-dependent proton transfer pathways in the photosynthetic cytochrome b6f complex.2013

    • Author(s)
      HasanS.S.,YamashitaE.,BaniulisD.,CramerW.A.
    • Journal Title

      Proc Natl Acad Sci USA

      Volume: 110 Pages: 4297-4302

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Crystal structure of the C-terminal domain of Mu phage central spike and functions of bound calcium ion.2013

    • Author(s)
      HaradaK.,YamashitaE.,NakagawaA.,MiyafusaT.,TsumotoK.,UenoT.,ToyamaY.,TakedaS.
    • Journal Title

      Biochim Biophys Acta

      Volume: 1834 Pages: 284-291

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Crystal structure of the C-terminal domain of Mu phage central spike and functions of bound calcium ion.2013

    • Author(s)
      Harada K
    • Journal Title

      Biochim Biophys Acta.,

      Volume: 1834 Issue: 1 Pages: 284-291

    • DOI

      10.1016/j.bbapap.2012.08.015

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Crystallization and preliminary X-ray diffraction analysis of human importin β-Snail zinc finger domain complex.2013

    • Author(s)
      Choi S., Song J., Son S.Y., Park I.Y., Yamashita E., Lee S.J..
    • Journal Title

      Acta Cryst. F

      Volume: 69 Issue: 6 Pages: 1049-1051

    • DOI

      10.1042/bst20120211

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Quinone-dependent proton transfer pathways in the photosynthetic cytochrome b6f complex.2013

    • Author(s)
      Hasan S. S.
    • Journal Title

      Proc Natl Acad Sci U S A.

      Volume: 110 Issue: 11 Pages: 4297-4302

    • DOI

      10.1073/pnas.1222248110

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Pathways of colicin import:utilization of BtuB,OmpF porin and the TolC drug-export protein.2012

    • Author(s)
      ZakharovS.D.,SharmaO.,ZhalninaM.V.,YamashitaE.,CramerW.A.
    • Journal Title

      Biochem.Soc.Trans.

      Volume: 40 Pages: 1463-1468

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Structural flexibility regulates phosphopeptide-binding activity of the tyrosine kinase binding domain of Cbl-c.2012

    • Author(s)
      Takesita K
    • Journal Title

      J. Biochem.

      Volume: 152 Issue: 5 Pages: 487-495

    • DOI

      10.1093/jb/mvs085

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Crystallization and preliminary X-ray crystallographic study of the human MST2 SARAH domain.2011

    • Author(s)
      SongJ.,HongH.,ChoiS.,LeeY.H.,YamashitaE.,BaeS.C.,ParkI.Y.,LeeS.J.
    • Journal Title

      Acta Cryst.

      Volume: F67 Pages: 1403-1405

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Conservation of Lipid Functions in Cytochrome bc Complexes2011

    • Author(s)
      HasanS.S.,YamashitaE.,RyanC.M.,WhiteleggeJ.P.,CramerW.A.
    • Journal Title

      J Mol.Biol.

      Volume: 414 Pages: 145-162

    • Related Report
      2012 Final Research Report 2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The host-binding domain of the P2 phage tail spike reveals a trimeric iron-binding structure.2011

    • Author(s)
      YamashitaE.,NakagawaA.,TakahashiJ.,TsunodaK.I.,YamadaS.,TakedaS.
    • Journal Title

      Acta Cryst.

      Volume: F67 Pages: 837-841

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Distinguishing between Cl(-) and O(2)(2-) as the bridging element between Fe(3+) and Cu(2+) in resting-oxidized cytochrome c oxidase.2011

    • Author(s)
      Suga M., Yano N., Muramoto K., Shinzawa-Itoh K., Maeda T., Yamashita E., Tsukihara T., Yoshikawa S.
    • Journal Title

      Acta Cryst.

      Volume: D67 Pages: 742-744

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Crystal structure of the cytoplasmic PTEN-like region of Ci-VSP provides insight into substrate specificity and redox regulation of the phosphoinositide phosphatase activity.2011

    • Author(s)
      Matsuda M., Takeshita K., Kurokawa T., Sakata S., Suzuki M., Yamashita E., Okamura Y., Nakagawa A.
    • Journal Title

      J. Biol. Chem.

      Volume: 286 Pages: 23368-23377

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] tructural insight into maintenance methylation by mouse DNA methyltransferase 1 (Dnmt1).2011

    • Author(s)
      Takeshita K., Suetake I., Yamashita E., Suga M., Narita H., Nakagawa A., Tajima S.
    • Journal Title

      Proc Natl Acad Sci U S A.

      Volume: 108 Pages: 9055-9059

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Selective nuclear export mechanism of small RNAs.2011

    • Author(s)
      Lee S. J., Jiko C., Yamashita E., Tsukihara T.
    • Journal Title

      Curr. Opin, Struc. Biol.

      Volume: 21 Pages: 101-108

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Structural insight into maintenance methylation by mouse DNA methyltransferase 1 (Dnmt1)2011

    • Author(s)
      Takeshita K., Yamashita E.
    • Journal Title

      Proc Natl Acad Sci USA

      Volume: 108 Pages: 9055-9059

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Crystal structure of the cytoplasmic PTEN-like region of Ci-VSP provides insight into substrate specificity and redox regulation of the phosphoinositide phosphatase activity2011

    • Author(s)
      Matsuda M.Yamashita E.
    • Journal Title

      J.Biol.Chem.

      Volume: 286 Pages: 23368-23377

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Distinguishing between Cl(-) and O(2)(2-) as the bridging element between Fe(3+) and Cu(2+) in resting-oxidized cytochrome c oxidase2011

    • Author(s)
      Suga M., Yamashita E.
    • Journal Title

      Acta Cryst

      Volume: D67 Pages: 742-744

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The host-binding domain of the P2 phage tail spike reveals a trimeric iron-binding structure2011

    • Author(s)
      Yamashita E.
    • Journal Title

      Ada Cryst

      Volume: F67 Pages: 837-841

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Crystallization and preliminary X-ray crystallographic study of the human MST2 SARAH domain2011

    • Author(s)
      Song J., Yamashita E.
    • Journal Title

      Acta Cryst

      Volume: F67 Pages: 1403-1405

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Selective nuclear export mechanism of small RNAs2011

    • Author(s)
      Lee S.J., Yamashita E.
    • Journal Title

      Curre.Opin.Strcut.Biol.

      Volume: 21 Pages: 101-108

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Presentation] Spring-8 生体超分子複合体構造解析ビームライン(大阪大学蛋白質研究所)BL44XU の現状2013

    • Author(s)
      山下栄樹
    • Organizer
      日本放射光学会
    • Place of Presentation
      名古屋大学
    • Year and Date
      2013-01-12
    • Related Report
      2012 Final Research Report
  • [Presentation] SPring-8生体超分子複合体構造解析ビームライン(大阪大学蛋白質研究所)BL44XUの現状2013

    • Author(s)
      山下栄樹
    • Organizer
      第26回日本放射光学会年会放射光科学合同シンポジウム
    • Place of Presentation
      名古屋大学
    • Related Report
      2012 Annual Research Report
  • [Presentation] 異物排出タンパク質のX線結晶構造解析2013

    • Author(s)
      板倉勇太
    • Organizer
      第26回日本放射光学会年会放射光科学合同シンポジウム
    • Place of Presentation
      名古屋大学
    • Related Report
      2012 Annual Research Report
  • [Presentation] Exportin-5 によるRNAsの核外輸送メカニズムの解明2012

    • Author(s)
      山澤龍治、山下栄樹
    • Organizer
      日本蛋白質科学会
    • Place of Presentation
      名古屋大学
    • Year and Date
      2012-06-22
    • Related Report
      2012 Final Research Report
  • [Presentation] Spring-8 生体超分子複合体構造解析ビームライン(大阪大学蛋白質研究所)BL44XU の現状2012

    • Author(s)
      山下栄樹
    • Organizer
      日本放射光学会
    • Place of Presentation
      鳥栖市民文化会館
    • Year and Date
      2012-01-08
    • Related Report
      2012 Final Research Report
  • [Presentation] SPring-8生体超分子複合体構造解析ビームライン(大阪大学蛋白質研究所)BL44XUの現状2012

    • Author(s)
      山下栄樹
    • Organizer
      第25回日本放射光学会年会放射光科学合同シンポジウム
    • Place of Presentation
      鳥栖市民文化会館・中央公民館
    • Year and Date
      2012-01-08
    • Related Report
      2011 Annual Research Report
  • [Presentation] Exportin-5による RNAs の核外輸送メカニズムの解明 / The structural basis of RNAs nuclear export by Exportin-5.2012

    • Author(s)
      山澤龍治
    • Organizer
      第12回 日本蛋白質科学会年会
    • Place of Presentation
      名古屋国際会議場
    • Related Report
      2012 Annual Research Report
  • [Presentation] pre-microRNA 核外輸送複合体のX 線結晶構造解析2010

    • Author(s)
      山下栄樹
    • Organizer
      BMB2010
    • Place of Presentation
      神戸国際会議場
    • Year and Date
      2010-12-10
    • Related Report
      2012 Final Research Report
  • [Presentation] pre-microRNA核外輸送複合体のX線結晶構造解析2010

    • Author(s)
      山下栄樹
    • Organizer
      BMB2010
    • Place of Presentation
      神戸ポートピアランド
    • Year and Date
      2010-12-10
    • Related Report
      2010 Annual Research Report
  • [Presentation] タンパク質合成を抑制する小さなRNA を輸送する仕組みの解明2010

    • Author(s)
      山下栄樹
    • Organizer
      SPring-8合同コンファレンス
    • Place of Presentation
      東京ステーションコンファレンス
    • Year and Date
      2010-11-04
    • Related Report
      2012 Final Research Report
  • [Presentation] タンパク質合成を抑制する小さなRNAを輸送する仕組みの解明2010

    • Author(s)
      山下栄樹
    • Organizer
      第2回SPring-8合同コンファレンス
    • Place of Presentation
      東京ステーションコンファレンス(招待講演)
    • Year and Date
      2010-11-04
    • Related Report
      2010 Annual Research Report
  • [Presentation] Nuclear translocation machinery of pre-microRNA.2010

    • Author(s)
      SooJaeLee、EikiYamashita
    • Organizer
      AsCA2010
    • Place of Presentation
      プサン(韓国)
    • Year and Date
      2010-11-01
    • Related Report
      2012 Final Research Report
  • [Presentation] microRNA核外輸送複合体のX線結晶構造解析2010

    • Author(s)
      慈幸千真理、山下栄樹
    • Organizer
      日本蛋白質科学会
    • Place of Presentation
      札幌コンベンションセンター
    • Year and Date
      2010-06-18
    • Related Report
      2012 Final Research Report
  • [Presentation] Spring-8 阪大蛋白研ビームラインBL44XU の現状2010

    • Author(s)
      山下栄樹
    • Organizer
      日本結晶学会
    • Place of Presentation
      大阪大学
    • Related Report
      2012 Final Research Report
  • [Book] X線結晶構造解析のためのタンパク質調製法、タンパク質実験ノート(上)改訂第4 版2011

    • Author(s)
      山下栄樹
    • Publisher
      羊土社
    • Related Report
      2012 Final Research Report
  • [Remarks]

    • URL

      http://www.protein.osaka-u.ac.jp/rcsfp/supracryst/jp/

    • Related Report
      2012 Final Research Report

URL: 

Published: 2010-08-23   Modified: 2019-07-29  

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