Analysis of self-renewal mechanisms of embryonic stem cells

• Project/Area Number: 22370050
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Functional biochemistry
• Research Institution: Kanazawa University
• Principal Investigator: YOKOTA Takashi 金沢大学, 医学系, 教授 (50134622)
• Co-Investigator(Kenkyū-buntansha): KOIDE Hiroshi 金沢大学, 医学系, 准教授 (70260536) ; AKAGI Tadayuki 金沢大学, 医学系, 助教 (70532183)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥18,720,000 (Direct Cost: ¥14,400,000、Indirect Cost: ¥4,320,000); Fiscal Year 2012: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000); Fiscal Year 2011: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000); Fiscal Year 2010: ¥6,760,000 (Direct Cost: ¥5,200,000、Indirect Cost: ¥1,560,000)
• Keywords: 細胞情報伝達機構 / 未分化性維持機構 / ES細胞 / 自己複製 / Dax1 / Oct3/4 / Esrrb / LRH1 / 幹細胞 / エピジェネティクス / 細胞周期 / クロマチンリモデリング / エピジェネティックス / クロマチン・リモデリング

Research Abstract

Mouse embryonic stem (ES) cells have two major characteristics: self-renewal capacity and pluripotency. Here, we focused on Eed (epigenetic controlling factor), Baf53a (chromatin remodeler), LRH-1 (cell cycle regulator), and transcription factor network (relationship among Oct3/4, Dax1 and Esrrb; and Gli). From the investigations of these factors, we revealed molecular mechanisms of self-renewal capacity of ES cells.

Research Products

• [Journal Article] Dax1 Associates with Esrrb and Regulates Its Function in Embryonic Stem Cells (2013)
  • Author(s): Uranishi K, Akagi T, Sun C, Koide H, Yokota T
  • Journal Title: Mol Cell Biol Volume: 33 Issue: 10 Pages: 2056-66
  • DOI: 10.1128/mcb.01520-12
  • Peer Reviewed
• [Journal Article] Inecalcitol, an analog of 1α,25(OH)(2) D(3) , induces growth arrest of androgen-dependent prostate cancer cells (2012)
  • Author(s): Okamoto R, Delansorne R, Wakimoto N, Doan NB, Akagi T, Shen M, Ho QH, Said JW, Koeffler HP
  • Journal Title: Int J Cancer Volume: 130 Issue: 10 Pages: 2464-73
  • DOI: 10.1002/ijc.26279
  • Peer Reviewed
• [Journal Article] RYBP-PRC1 complexes mediate H2A ubiquitylation at polycomb target sites independently of PRC2 and H3K27me3 (2012)
  • Author(s): Tavares L, Dimitrova E, Oxley D, Webster J, Poot R, Demmers J, Bezstarosti K, Taylor S, Ura H, Koide H, Wutz A, Vidal M, Elderkin S, Brockdorff N
  • Journal Title: Cell Volume: 148 Issue: 4 Pages: 664-78
  • DOI: 10.1016/j.cell.2011.12.029
  • Peer Reviewed
• [Journal Article] β-catenin enhances Oct-4 activity and reinforces pluripotency through a TCF-independent mechanism (2011)
  • Author(s): Kelly KF, Ng DY, Jayakumaran G, Wood GA, Koide H, Doble BW
  • Journal Title: Cell Stem Cell Volume: 8 Issue: 2 Pages: 214-27
  • DOI: 10.1016/j.stem.2010.12.010
  • Peer Reviewed
• [Journal Article] Eed/Sox2 regulatory loop controls ES cell self-renewal through histone methylation and acethylation (2011)
  • Author(s): Ura, H.
  • Journal Title: EMBO J Volume: 30 Issue: 11 Pages: 2190-2204
  • DOI: 10.1038/emboj.2011.126
  • Peer Reviewed
• [Journal Article] マウスES細胞の自己複製維持におけるDax1とEsrrbの相互作用 (2011)
  • Author(s): 浦西洸介
  • Journal Title: 金沢大学十全医学会雑誌 Volume: 第120巻第2号 Pages: 60-61
• [Journal Article] Eed/Sox2 regulatory loop controls ES cell self-renewal through histone methylation and acetylation. (2011)
  • Author(s): Ura H., et al.
  • Journal Title: EMBO Journal Volume: (印刷中)
  • Peer Reviewed
• [Journal Article] β-Catenin Enhances Oct-4 Activity and Reinforces Pluripotency through a TCF-Independent Mechanism. (2011)
  • Author(s): Kelly, K.F., et al.
  • Journal Title: Cell Stem Cell Volume: 8 Pages: 214-227
  • Peer Reviewed
• [Journal Article] In vivo deficiency of both C/EBPβ and C/EBPε results in highly defective myeloid differentiation and lack of cytokine response (2010)
  • Author(s): Akagi T, Thoennissen NH, George A, Crooks G, Song JH, Okamoto R, Nowak D, Gombart AF, Koeffler HP
  • Journal Title: PLoS One Volume: 5 Issue: 11 Pages: e15419-e15419
  • DOI: 10.1371/journal.pone.0015419
  • NAID: 120002647977
  • Peer Reviewed
• [Journal Article] A stem cell-derived gene (Sddr) negatively regulates differentiation of embryonic stem cells (2010)
  • Author(s): Miura M, Ueda A, Takao Y, Nishimura EK, Koide H, Yokota T
  • Journal Title: Int J Dev Biol Volume: 54 Issue: 1 Pages: 33-9
  • DOI: 10.1387/ijdb.082802mm
  • NAID: 120001939016
  • Peer Reviewed
• [Journal Article] Genomic profiling of adult acute lymphoblastic leukemia by single nucleotide polymorphism oligonucleotide microarray and comparison to pediatric acute lymphoblastic leukemia (2010)
  • Author(s): Okamoto R, Ogawa S, Nowak D, Kawamata N, Akagi T, Kato M, Sanada M, Weiss T, Haferlach C, Dugas M, Ruckert C, Haferlach T, Koeffler HP
  • Journal Title: Haematologica. Volume: 95 Issue: 9 Pages: 1481-8
  • DOI: 10.3324/haematol.2009.011114
  • Peer Reviewed
• [Journal Article] 正常核型急性骨髄性白血病のSNP アレイ解析 (2010)
  • Author(s): 赤木紀之
  • Journal Title: 血液フロンティア(医薬ジャーナル社) Volume: 20 Pages: 31-37
  • URL: https://www.iyaku-j.com/iyakuj/system/M2-1/summary_viewer.php?trgid=22011
• [Journal Article] In vivo deficiency of both C/EBPB and C/EBPe results in highly defective myeloid differentiation and lack of cytokine response. (2010)
  • Author(s): Akagi T., et al.
  • Journal Title: PLoS One Volume: 5
  • Peer Reviewed
• [Presentation] Zfp296のマウスES細胞における役割 (2012)
  • Author(s): 藤井優佳,懸川まどか,赤木紀之,小出寛,横田崇
  • Organizer: 第35回日本分子生物学会
  • Place of Presentation: 福岡県・福岡国際会議場
• [Presentation] マウスES細胞におけるLRH-1の増殖制御機構の解析 (2012)
  • Author(s): 金井大,武石健,上田篤,赤木紀之,小出寛,横田崇
  • Organizer: 第85回日本生化学会大会
  • Place of Presentation: 福岡県・福岡国際会議場
• [Presentation] INVOLVEMENT OF ETS-RELATED TRANSCRIPTION FACTORS ETV4 AND ETV5 IN PLURIPOTENCY AND PROLIFERATION OF MOUSE EMBRYONIC STEM CELLS (2012)
  • Author(s): Tadayuki Akagi, Satu Kuure, Hiroshi Koide, Frank Costantini, Takashi Yokota
  • Organizer: ISSCR 10th Annual Meeting
  • Place of Presentation: 神奈川県・パシフィコ横浜
• [Presentation] DAX1 ASSOCIATES WITH ESRRB AND FUNCTIONS AS A REPRESSOR IN EMBRYONIC STEM CELLS (2012)
  • Author(s): Kousuke Uranishi, Tadayuki Akagi, Chuanhai Sun, Hiroshi Koide, and Takashi Yokota
  • Organizer: I SSCR 10th Annual Meeting
  • Place of Presentation: 神奈川県・パシフィコ横浜
• [Presentation] NANOG/ZFP57 PATHWAY PROMOTES ANCHORAGE INDEPENDENT GROWTH OF HT1080 CELLS BY INDUCING IMPRINTED GENES EXPRESSION (2012)
  • Author(s): Tada Yuhki, Tadayuki Akagi, Takashi Yokota, and Hiroshi Koide
  • Organizer: I SSCR 10th Annual Meeting
  • Place of Presentation: 神奈川県・パシフィコ横浜
• [Presentation] Zfp296のマウスES細胞における役割 (2012)
  • Author(s): 藤井優佳，懸川まどか，赤木紀之，小出寛，横田崇.
  • Organizer: 第35回 日本分子生物学会
  • Place of Presentation: 福岡県・福岡国際会議場
• [Presentation] マウスES細胞におけるLRH-1の増殖制御機構の解析 (2012)
  • Author(s): 金井大，武石健，上田篤，赤木紀之，小出寛，横田崇.
  • Organizer: 第85回 日本生化学会大会
  • Place of Presentation: 福岡県・福岡国際会議場
• [Presentation] INVOLVEMENT OF ETS-RELATED TRANSCRIPTION FACTORS ETV4 AND ETV5 IN PLURIPOTENCY AND PROLIFERATION OF MOUSE EMBRYONIC STEM CELLS. (2012)
  • Author(s): Tadayuki Akagi, Satu Kuure, Hiroshi Koide, Frank Costantini, Takashi Yokota.
  • Organizer: ISSCR 10th Annual Meeting
  • Place of Presentation: 神奈川県・パシフィコ横浜
• [Presentation] DAX1 ASSOCIATES WITH ESRRB AND FUNCTIONS AS A REPRESSOR IN EMBRYONIC STEM CELLS. (2012)
  • Author(s): Kousuke Uranishi, Tadayuki Akagi, Chuanhai Sun, Hiroshi Koide, and Takashi Yokota.
  • Organizer: ISSCR 10th Annual Meeting.
  • Place of Presentation: 神奈川県・パシフィコ横浜
• [Presentation] NANOG/ZFP57 PATHWAY PROMOTES ANCHORAGE INDEPENDENT GROWTH OF HT1080 CELLS BY INDUCING IMPRINTED GENES EXPRESSION. (2012)
  • Author(s): Tada Yuhki, Tadayuki Akagi, Takashi Yokota, and Hiroshi Koide.
  • Organizer: ISSCR 10th Annual Meeting.
  • Place of Presentation: 神奈川県・パシフィコ横浜
• [Presentation] 未分化なマウスES細胞におけるOct3/4、Dax1、Esrrbの相互作用 (2011)
  • Author(s): 浦西 洸介、赤木 紀之、孫 伝海、小出 寛、横田 崇
  • Organizer: 日本生化学会北陸支部第29回大会
  • Place of Presentation: 石川県・金沢大学
  • Year and Date: 2011-05-28
• [Presentation] Nanogはヒト繊維肉腫HT1080細胞の足場非依存性増殖に必要である (2011)
  • Author(s): 田多 祐喜, 赤木 紀之, 横田 崇, 小出寛
  • Organizer: 日本生化学会北陸支部第29回大会
  • Place of Presentation: 石川県・金沢大学
  • Year and Date: 2011-05-28
• [Presentation] 未分化なマウスES細胞におけるOct3/4、Dax1、Esrrbの相互作用 (2011)
  • Author(s): 浦西洸介, ら
  • Organizer: 日本生化学会北陸支部第29回大会
  • Place of Presentation: 金沢大学(石川県)
  • Year and Date: 2011-05-28
• [Presentation] Nanogはヒト繊維肉腫HT1080細胞の足場非依存性増殖に必要である (2011)
  • Author(s): 田多祐喜, ら
  • Organizer: 日本生化学会北陸支部第29回大会
  • Place of Presentation: 金沢大学(石川県)
  • Year and Date: 2011-05-28
• [Presentation] ES細胞の自己複製維持における転写因子とエピジェネティックス制御因子のクロストーク (2011)
  • Author(s): 小出寛
  • Organizer: 第10回日本再生医療学会総会
  • Place of Presentation: 京王プラザホテル(東京都) 招待講演
  • Year and Date: 2011-03-02
• [Presentation] Ets-related transcription factors ETV4/5 are involved in pluripotency and proliferation of mouse ES cells (2011)
  • Author(s): Tadayuki Akagi,Satu Kuure,Hiroshi Koide,Frank Costantini,Takashi Yokota
  • Organizer: 第34回日本分子生物学会
  • Place of Presentation: 神奈川県・パシフィコ横浜
• [Presentation] マウスES細胞の自己複製維持におけるOct3/4、Dax1、Esrrbの相互作用 (2011)
  • Author(s): 浦西 洸介,赤木 紀之,孫 伝海,小出 寛,横田 崇
  • Organizer: 第34回日本分子生物学会
  • Place of Presentation: 神奈川県・パシフィコ横浜
• [Presentation] 癌細胞におけるES 細胞特異的遺伝子Zfp57の機能解析 (2011)
  • Author(s): 田多 祐喜,赤木 紀之,横田 崇,小出 寛
  • Organizer: 第34回日本分子生物学会
  • Place of Presentation: 神奈川県・パシフィコ横浜
• [Presentation] Ets-related transcription factors ETV4 and ETV5 are involved in pluripotency and proliferation of mouse embryonic stem cells (2011)
  • Author(s): Tadayuki Akagi, Satu Kuure, Hiroshi Koide, Frank Costantini, Takashi Yokota
  • Organizer: 日本分子生物学会第11回春季シンポジウム
  • Place of Presentation: 石川県・石川県立音楽堂
• [Presentation] Eed and Sox2 form regulatory loop that regulates ES cell self-renewal by modulating histon methylation and acetylation (2011)
  • Author(s): Hiroki Ura, Kazuhiro Murakami, Tadayuki Akagi, Keita Kinoshita, Shukuro Yamaguchi, Shinji Masui, Hitoshi Niwa, Hiroshi Koide, Takashi Yokota
  • Organizer: 日本分子生物学会第11回春季シンポジウム
  • Place of Presentation: 石川県・石川県立音楽堂
• [Presentation] マウスES細胞におけるOct3/4、Dax1、Esrrbの相互作用 (2011)
  • Author(s): 浦西 洸介、赤木 紀之、孫 伝海、小出 寛、横田 崇
  • Organizer: 日本分子生物学会第11回春季シンポジウム
  • Place of Presentation: 石川県・石川県立音楽堂
• [Presentation] Nanog regulates anchorage-independent growth of HT1080 fibrosarcoma cells (2011)
  • Author(s): Yuhki Tada, Tadayuki Akagi, Takashi Yokota, Hiroshi Koide
  • Organizer: 日本分子生物学会第11回春季シンポジウム
  • Place of Presentation: 石川県・石川県立音楽堂
• [Presentation] ヒト繊維芽肉腫HT1080 細胞の増殖におけるPDGF-Cの関与 (2011)
  • Author(s): 金城 智章, 横田 崇, 小出 寛
  • Organizer: 日本分子生物学会第11回春季シンポジウム
  • Place of Presentation: 石川県・石川県立音楽堂
• [Presentation] Involvement of nuclear hormone receptors Dax1 and Esrrb in the maintenance of self-renewal in ES cells (2011)
  • Author(s): Kousuke Uranishi, Tadayuki Akagi, Chuanhai Sun, Hiroshi Koide, Takashi Yokota
  • Organizer: The 9th Stem Cell Research Symposium
  • Place of Presentation: 東京都・泉ガーデンギャラリー
• [Presentation] Eed/Sox2 regulatory loop controls ES cell self-renewal through histone methylation and acetylation (2011)
  • Author(s): Hiroshi Koide, Hiroki Ura, Kazuhiro Murakami, Tadayuki Akagi, Keita Kinoshita, Shukuro Yamaguchi, Shinji Masui, Hitoshi Niwa, Takashi Yokota
  • Organizer: The 9th Stem Cell Research Symposium
  • Place of Presentation: 東京都・泉ガーデンギャラリー
• [Presentation] anog regulates anchorage-independent growth of HT1080 fibrosarcoma cells (2011)
  • Author(s): Yuhki Tada, Tadayuki Akagi, Takashi Yokota, Hiroshi Koide
  • Organizer: The 9th Stem Cell Research Symposium
  • Place of Presentation: 東京都・泉ガーデンギャラリー
• [Presentation] Ets-related transcription factors ETV4/5 are involved in pluripotency and proliferation of mouse ES cells (2011)
  • Author(s): 赤木紀之, ら
  • Organizer: 第34回日本分子生物学会
  • Place of Presentation: パシフィコ横浜(神奈川県)
• [Presentation] マウスES細胞の自己複製維持におけるOct3/4、Dax1、Esrrbの相互作用 (2011)
  • Author(s): 浦西洸介, ら
  • Organizer: 第34回日本分子生物学会
  • Place of Presentation: パシフィコ横浜(神奈川県)
• [Presentation] 癌細胞におけるES細胞特異的遺伝子Zfp57の機能解析 (2011)
  • Author(s): 田多祐喜, ら
  • Organizer: 第34回日本分子生物学会
  • Place of Presentation: パシフィコ横浜(神奈川県)
• [Presentation] Ets-related transcription factors ETV4 and ETV5 are involved in pluripotency and proliferation of mouse embryonic stem cells (2011)
  • Author(s): 赤木紀之, ら
  • Organizer: 日本分子生物学会 第11回春季シンポジウム
  • Place of Presentation: 石川県立音楽堂(石川県)
• [Presentation] Eed and Sox2 form regulatory loop that regulates ES cell self-renewal by modulating histon methylation and acetylation (2011)
  • Author(s): 浦大樹, ら
  • Organizer: 日本分子生物学会 第11回春季シンポジウム
  • Place of Presentation: 石川県立音楽堂(石川県)
• [Presentation] マウスES細胞におけるOct3/4、Dax1、Esrrbの相互作用 (2011)
  • Author(s): 浦西洸介, ら
  • Organizer: 日本分子生物学会 第11回春季シンポジウム
  • Place of Presentation: 石川県立音楽堂(石川県)
• [Presentation] Nanog regulates anchorage-independent growth of HT1080 fibrosarcoma cells (2011)
  • Author(s): 田多祐喜, ら
  • Organizer: 日本分子生物学会 第11回春季シンポジウム
  • Place of Presentation: 石川県立音楽堂(石川県)
• [Presentation] ヒト繊維肉腫HT1080細胞の増殖におけるPDGF-Cの関与 (2011)
  • Author(s): 金城智章, ら
  • Organizer: 日本分子生物学会 第11回春季シンポジウム
  • Place of Presentation: 石川県立音楽堂(石川県)
• [Presentation] Involvement of nuclear hormone receptors Dax1 and Esrrb in the maintenance of self-renewal in ES cells (2011)
  • Author(s): 浦西洸介, ら
  • Organizer: The 9th Stem Cell Research Symposium
  • Place of Presentation: 泉ガーデンギャラリー(東京都)
• [Presentation] Eed/Sox2 regulatory loop controls ES cell self-renewal through histone methylation and acetylation (2011)
  • Author(s): 小出寛, ら
  • Organizer: The 9th Stem Cell Research Symposium
  • Place of Presentation: 泉ガーデンギャラリー(東京都)
• [Presentation] Nanog regulates anchorage-independent growth of HT1080 fibrosarcoma cells (2011)
  • Author(s): 田多祐喜, ら
  • Organizer: The 9th Stem Cell Research Symposium
  • Place of Presentation: 泉ガーデンギャラリー(東京都)
• [Presentation] マウ スES細胞の自己複製や分化にはEedの適切な発現レベルが必要である (2010)
  • Author(s): 小出 寛
  • Organizer: 十全医学会学術集会
  • Place of Presentation: 石川県・金沢大学十全講堂
  • Year and Date: 2010-07-09
• [Presentation] ES細胞におけるGli2の発現制御機構の解析 (2010)
  • Author(s): 上田 篤、田多 祐喜、浦 大樹、赤木 紀之、小出 寛、横田 崇
  • Organizer: 日本生化学会北陸支部第28回大会
  • Place of Presentation: 福井県・福井大学
  • Year and Date: 2010-05-29
• [Presentation] ヒト繊維肉腫HT1080細胞の増殖機構におけるNanogの役割 (2010)
  • Author(s): 田多 祐喜、赤木 紀之、 横田 崇、 小出寛
  • Organizer: 日本生化学会北陸支部第28回大会
  • Place of Presentation: 福井県・福井大学
  • Year and Date: 2010-05-29
• [Presentation] ES細胞におけるGli2の発現制御機構の解析 (2010)
  • Author(s): 上田篤, ら
  • Organizer: 日本生化学会北陸支部第28回大会
  • Place of Presentation: 福井大学(福井県)
  • Year and Date: 2010-05-29
• [Presentation] ヒト繊維肉腫HT1080細胞の増殖機構におけるNanogの役割 (2010)
  • Author(s): 田多祐喜, ら
  • Organizer: 日本生化学会北陸支部第28回大会
  • Place of Presentation: 福井大学(福井県)
  • Year and Date: 2010-05-29
• [Presentation] 未分化なES細胞特異的に発現しているDax1 とEsrrb の相互作用 (2010)
  • Author(s): 浦西 洸介,赤木 紀之,孫 伝海,小出 寛,横田 崇
  • Organizer: BMB2010
  • Place of Presentation: 兵庫県・神戸ポートアイランド
• [Presentation] Nanog is essential for anchorage-independent growth of HT1080 fibrosarcoma cells (2010)
  • Author(s): 田多 祐喜,赤木 紀之,横田 崇,小出寛
  • Organizer: BMB2010
  • Place of Presentation: 兵庫県・神戸ポートアイランド
• [Presentation] マウスES細胞の分化にはEedの適切な発現レベルが必要である (2010)
  • Author(s): 小出 寛、横田 崇
  • Organizer: 第31回日本炎症・再生医学会
  • Place of Presentation: 東京都・京王プラザホテル
• [Presentation] マウスES細胞の分化にはEedの適切な発現レベルが必要である (2010)
  • Author(s): 小出寛, ら
  • Organizer: 第31回日本炎症・再生医学会
  • Place of Presentation: 京王プラザホテル(東京都)
• [Presentation] 未分化なES細胞特異的に発現しているDax1とEsrrbの相互作用 (2010)
  • Author(s): 浦西洸介, ら
  • Organizer: BMB2010
  • Place of Presentation: 神戸ポートアイランド(兵庫県)
• [Presentation] Nanog is essential for anchorage-independent growth of HT1080 fibrosarcoma cells (2010)
  • Author(s): 田多祐喜, ら
  • Organizer: BMB2010
  • Place of Presentation: 神戸ポートアイランド(兵庫県)
• [Book] The LIF/STAT3 Pathway in ES Cell Self-renewal in Embryonic Stem Cells. The Hormonal Regulation of Pluripotency and Embryogenesis (2011)
  • Author(s): Hiroshi Koide and Takashi Yokota
  • Publisher: Craig S. Atwood, ed.
• [Book] The LIF/STAT3 Pathway in ES Cell Self-renewal in Embryonic Stem Cells. The Hormonal Regulation of Pluripotency and Embryogenesis. (Craig S.Atwood, ed.) pp 61-78 (2011)
  • Author(s): 小出寛、横田崇
  • Total Pages: 672
  • Publisher: InTech
• [Book] Vitamin D and Hematologic Malignancies (Chapter 11) In: "Vitamin D and Cancer (1st edition) (2010)
  • Author(s): Okamoto R, Akagi T, Koeffler HP
  • Publisher: (Donald L. Trump and Candace S. Johnson eds)
• [Remarks] ホームページ
  • URL: http://medstem.w3.kanazawa-u.ac.jp
• [Remarks] Atsushi Ueda. Involvement of Gli proteins in undifferentiated state maintenance and proliferation of embryonic stem cells. 金沢大学十全医学会雑誌 第121巻2号38-46(2012)
• [Remarks] 浦西洸介、マウスES細胞の自己複製維持におけるDax1とEsrrbの相互作用、金沢大学十全医学会雑誌 第120巻 第2号 60-61(2011)
• [Remarks] 金沢大学大学院医学系研究科 がん医科学専攻 再生分子医学研究室
  • URL: http://medstem.w3.kanazawa-u.ac.jp/
• [Remarks]
  • URL: http://medstem.w3.kanazawa-u.ac.jp/
• [Remarks]
  • URL: http://web.kanazawa-u.ac.jp/~med54/