| Budget Amount *help |
¥18,590,000 (Direct Cost: ¥14,300,000、Indirect Cost: ¥4,290,000)
Fiscal Year 2012: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000)
Fiscal Year 2011: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000)
Fiscal Year 2010: ¥6,110,000 (Direct Cost: ¥4,700,000、Indirect Cost: ¥1,410,000)
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| Research Abstract |
Protein degradation via ubiquitin-proteasome system regulates a variety of cellular processes. In budding yeast, the cullin protein family includes three members: Cdc53, Cul3, and Cul8. Recently we have reported Lag2 as a novel regulator of SCF complex. In this study, we performed the functional analysis of Lag2, Cul3 and Cul8. We identify that a single lysine, K16, on Lag2 is a target of Rub1 conjugation and Dcn1 is required for this reaction. Like wild-type Lag2, Lag2 K16R mutant also negatively modulates the SCF function, indicating that Rub1 conjugation to Lag2 is not required for the function of Lag2. In budding yeast, the BTB protein family includes four members. We identify a BTB protein, Yil001w as a Cul3 binding protein and Okp1 as a candidate of Cul3-type E3. Furthermore, we identify several proteins as Cul8 binding proteins. These observations indicate that Cul3 and Cul8 form complexes and function as E3 ubiquitin ligases.
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