Mechanism of genome DNA maintenance methylation
| Project/Area Number |
22370053
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Functional biochemistry
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| Research Institution | Osaka University |
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Principal Investigator |
TAJIMA Shoji 大阪大学, 蛋白質研究所, 教授 (50132931)
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| Co-Investigator(Kenkyū-buntansha) |
NAKAGAWA Atsushi 大阪大学, 蛋白質研究所, 教授 (20188890)
SUETAKE Isao 大阪大学, 蛋白質研究所, 准教授 (80304054)
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| Project Period (FY) |
2010 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥18,980,000 (Direct Cost: ¥14,600,000、Indirect Cost: ¥4,380,000)
Fiscal Year 2012: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2011: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2010: ¥9,100,000 (Direct Cost: ¥7,000,000、Indirect Cost: ¥2,100,000)
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| Keywords | DNA メチル化 / DNA メチルトランスフェラーゼ / 維持メチル化 / 生化学 / 構造生物学 / 酵素学 / ゲノムメチル化 / DNAメチルトランスフェラーゼ / DNAメチル化 / Dnmt1 / X線結晶構造解析 / 酵素 / ゲノム / 発現制御 / エピジェネティクス |
|---|
| Research Abstract |
Vertebrate genome is often methylated. DNA methylation is one of the factors that suppresses gene expression, and is crucial for development. DNA methylation patterns of genome are inherited to next generation during replication. DNA methyltransferase called Dnmt1 is responsible for faithful propagation of DNA methylation patterns. In the project we have provided structural and biochemical insights into the maintenance methylation
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Report
(4 results)
Research Products
(65 results)
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[Journal Article] Structural basis of the versatile DNA recognition ability of the methyl-CpG binding domain of methyl-CpG binding domain protein 4.2013
Author(s)
Otani, J., Arita, K., Kato, T., Kinoshita, M., Kimura, H., Suetake, I., Tajima, S., Ariyoshi, M., Shirakawa, M.
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Journal Title
J Biol. Chem.
Volume: 288
Issue: 9
Pages: 6351-6362
DOI
Related Report
Peer Reviewed
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[Journal Article] Hinge and chromoshadow of HP1α participate in recognition of K9 methylated histone H3 in nucleosomes.2012
Author(s)
Mishima Y, Watanabe M, Kawakami T, Jayasinghe CD, Otani J, Kikugawa Y, Shirakawa M, Kimura H, Nishimura O, Aimoto S, Tajima S, Suetake I.
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Journal Title
J Mol Biol.
Volume: 425
Issue: 1
Pages: 54-70
DOI
Related Report
Peer Reviewed
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[Journal Article] Genome-wide analysis of DNA methylation and expression of microRNAs in breast cancer cells.2012
Author(s)
Morita, S., Takahashi, R., Yamashita, R.,Toyoda, A., Horii, T., Kimura, M.,Fujiyama, A., Nakai, K., Tajima, S.,Matoba, R., Ochiya, T., and Hatada, I
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Journal Title
Int. J. Mol. Sci.
Volume: 13
Issue: 7
Pages: 8259-8272
DOI
Related Report
Peer Reviewed
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[Journal Article] The Dnmt3b splice variant is specifically expressed in in vitro-manipulated blastocysts and their derivative ES cells.2011
Author(s)
Horii, T., Suetake, I., Yanagisawa, E., Morita, S., Kimura, M., Nagao, Y., Imai,H., Tajima, S., and Hatada, I
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Journal Title
J. Reprod. Dev
Volume: 57
Pages: 579-585
NAID
Related Report
Peer Reviewed
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[Journal Article] Characterization of DNA-binding activity in the N-terminal domain of the DNA methyltransferase Dnmt3a2011
Author(s)
Suetake, I., Mishima, Y., Kimura, H., Lee, Y. H., Goto, Y., Takeshima, H., Ikegami, T., and Tajima, S.
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Journal Title
Biochem. J.
Volume: 437
Issue: 1
Pages: 141-148
DOI
Related Report
Peer Reviewed
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[Journal Article] Array-based genomic resequencing of human leukemia2010
Author(s)
Yamashita Y, Yuan J, Suetake I, Suzuki H, Ishikawa Y, Choi Y L, Ueno T, Soda M, Hamada T, Haruta H, Takada S, Miyazaki Y, Kiyoi H, Ito E, Naoe T, Tomonaga M, Toyota M, Tajima S, Iwama A, and Mano H
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Journal Title
Oncogene
Volume: 29
Issue: 25
Pages: 3723-3731
DOI
Related Report
Peer Reviewed
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