| Budget Amount *help |
¥18,590,000 (Direct Cost: ¥14,300,000、Indirect Cost: ¥4,290,000)
Fiscal Year 2014: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
Fiscal Year 2013: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Fiscal Year 2012: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
Fiscal Year 2011: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2010: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000)
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| Outline of Final Research Achievements |
Heme-copper oxygen reductase in respiratory electron transport chain efficiently converts energy released in the reduction reaction of molecular oxygen (electron transfer) to driving force for the active transport of hydrogen ion (proton pump). 1) binding manner of molecular oxygen, 2) O-O bond cleavage mechanism, 3) reaction intermediate structure, 4) the resting form structure, 5) structural change coupled to electron transfer were found based on the x-ray crystal structures of aa3-type, which belongs to the heme-copper oxygen reductase family. Purification method of cbb3-type was established, and property of the electron transfer efficiency was found by comparing the enzymatic activity of cbb3-type with that of aa3-type.
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