Analysis of RACK function in specific nascent peptide-dependent translation arrest
Project/Area Number |
22370062
|
Research Category |
Grant-in-Aid for Scientific Research (B)
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Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Molecular biology
|
Research Institution | Tohoku University |
Principal Investigator |
INADA Toshifumi 東北大学, 大学院・薬学研究科, 教授 (40242812)
|
Co-Investigator(Renkei-kenkyūsha) |
HOSHINO Shin-ichi 名古屋市立大学, 大学院・薬学研究科, 教授 (40219168)
|
Project Period (FY) |
2010 – 2012
|
Project Status |
Completed (Fiscal Year 2012)
|
Budget Amount *help |
¥18,720,000 (Direct Cost: ¥14,400,000、Indirect Cost: ¥4,320,000)
Fiscal Year 2012: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000)
Fiscal Year 2011: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000)
Fiscal Year 2010: ¥6,760,000 (Direct Cost: ¥5,200,000、Indirect Cost: ¥1,560,000)
|
Keywords | mRNA 品質管理 / 翻訳アレスト / 新生ポリペプチド鎖 / mRNA 分子内切断 / ユ ビキチン化 / プロテアソーム / リボソーム / リボソーム解離因子 / mRNA品質管理 / 異常RNA分解 / 異常タンパク質分解 / ユビキチン化 / RNA / 翻訳 / 品質管理 / タンパク質分解 |
Research Abstract |
The receptor for activated C kinase (RACK1) was identified as a factor required for nascent peptide-dependent translation arrest, and the binding of RACK1 to 40S subunit is crucial for translation repression by poly-basic amino acid sequence. Two E3 ubiquitin ligases, Ltn1 and Not4, are involved in proteasomal protein degradation coupled to translation arrest, and translation arrest by RACK1 is required for Ltn1-depepdnet protein degradation.
|
Report
(4 results)
Research Products
(35 results)
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[Journal Article] A ribosome-bound quality control complex triggers nascent peptides and signals translation stress2012
Author(s)
Brandman, O., Ornstein, JS., Wong, D., Larson, A., Williams, C. C, Li, GW., Zhou, S., King, D., Shen, P S, Weibezahn, J., Dunn, J.q Rouskin, S., Inada, T., Frost, A., Weissman, JS
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Journal Title
Cell
Volume: 151
Issue: 5
Pages: 1042-1054
DOI
Related Report
Peer Reviewed
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[Journal Article] RACK1 stimulates nascent polypeptide-dependent translation arrest2010
Author(s)
Kuroha, K., Akamatsu, M., Dimitrova, L, lto, T.,Kato, Y Shirahige, K. and lnada. T
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Journal Title
EMBO. Rep
Volume: 11
Issue: 12
Pages: 956-961
DOI
Related Report
Peer Reviewed
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[Journal Article] RACK1 stimulates nascent polypeptide-dependent translation arrest2010
Author(s)
Kuroha, K., Akamatsu, M., Dimitrova, L., Ito, T., Kato, Y.Shirahige, K., Inada, T.
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Journal Title
EMBO Reports
Volume: 11
Pages: 956-961
Related Report
Peer Reviewed
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[Journal Article] Structural Basis for mRNA Surveillance by Archaeal Pelota and GTP-bound EF1α Complex2010
Author(s)
Kobayashi, K., Kikuno, I., Kuroha, K., Saito, K., Ito, K., Ishitani, R., Inada, T., Nureki, O.
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Journal Title
Proc.Natl.Acad.Sci.USA
Volume: 107
Pages: 17575-17579
Related Report
Peer Reviewed
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[Journal Article] Dual functions of yeast tRNA ligase in the unfolded protein response : unconventional cytoplasmic splicing of HAC1 pre-mRNA is not sufficient to release translational attenuation2010
Author(s)
Mori, T., Ogasawara, C., Inada, T., Englert, M., Beier, H., Takezawa, M., Endo, T., Yoshihisa, T.
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Journal Title
Mol.Biol.Cell
Volume: 21
Pages: 3722-3734
Related Report
Peer Reviewed
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