Live cell imaging of histone methylation and inactive X chromosome dynamics
Project/Area Number |
22370063
|
Research Category |
Grant-in-Aid for Scientific Research (B)
|
Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Molecular biology
|
Research Institution | Osaka University |
Principal Investigator |
KIMURA Hiroshi 大阪大学, 生命機能研究科, 准教授 (30241392)
|
Project Period (FY) |
2010-04-01 – 2013-03-31
|
Project Status |
Completed (Fiscal Year 2013)
|
Budget Amount *help |
¥18,590,000 (Direct Cost: ¥14,300,000、Indirect Cost: ¥4,290,000)
Fiscal Year 2012: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000)
Fiscal Year 2011: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000)
Fiscal Year 2010: ¥6,110,000 (Direct Cost: ¥4,700,000、Indirect Cost: ¥1,410,000)
|
Keywords | 染色体動態 / エピジェネティクス制御 / クロマチン / ヒストン修飾 / 生細胞イメージング / 生体イメージング / 転写制御 / 染色体 / モノクローナル抗体 / エピジェネティクス / ヒストンアセチル化 / 生細胞計測 / ヒストン / 翻訳後修飾 / 遺伝子発現 / 不活性X染色体 |
Research Abstract |
In order to visualize dynamics of inactive X chromosome, we developed two methods monitoring histone modifications in living cells. The first method uses fluorescently labeled antigen-binding fragment (Fab), which binds to endogenous modifications when loaded into living cells. Using this technique, we were able to track the behavior of inactive X chromosomes, where trimethylated histone H3K27me3 is enriched, during the cell cycle. The second method is a genetically encoded system using a single-chain variable fragment tagged with the green fluorescent protein. These methods will be useful to track epigenetic changes during development and differentiation.
|
Report
(4 results)
Research Products
(73 results)
-
[Journal Article] Genetically encoded system to track histone modification in vivo.2013
Author(s)
Yuko Sato, Masanori Mukai, Jun Ueda, Michiko Muraki, Timothy J. Stasevich, Naoki Horikoshi, Tomoya Kujirai, Hiroaki Kita, Taisuke Kimura, Seiji Hira, Yasushi Okada, Yoko Hayashi-Takanaka, Chikashi Obuse, Hitoshi Kurumizaka, Atsuo Kawahara, Kazuo Yamagata, Naohito Nozaki and Hiroshi Kimura
-
Journal Title
Scientific Reports
Volume: 3
Issue: 1
Pages: 2436-2436
DOI
Related Report
Peer Reviewed / Open Access
-
-
[Journal Article] Chromosome engineering allows the efficient isolation of vertebrate neocentromeres2013
Author(s)
Shang WH, Hori T, Martins NM, Toyoda A, Misu S, Monma N, Hiratani I, Maeshima K, Ikeo K, Fujiyama A, Kimura H, Earnshaw WC, and Fukagawa T
-
Journal Title
Dev Cell
Volume: 24
Issue: 6
Pages: 635-648
DOI
Related Report
Peer Reviewed
-
-
-
[Journal Article] Nap1 regulates proper CENP-B binding to nucleosomes2013
Author(s)
Tachiwana H, Miya Y, Shono N, Ohzeki J, Osakabe A, Otake K, Larionov V, Earnshaw WC, Kimura H, Masumoto H, Kurumizaka H
-
Journal Title
Nucleic Acids Res
Volume: 5
Issue: 5
Pages: 2869-2880
DOI
Related Report
Peer Reviewed
-
-
[Journal Article] Posttranscriptional Regulation of Histone Lysine Methyltransferase GLP in Embryonic Male Mouse Germ Cells.2013
Author(s)
Deguchi K, Nagamatsu G, Miyachi H, Kato Y, Morita S, Kimura H, Kitano S, Hatada I, Saga Y, Tachibana M, Shinkai Y.
-
Journal Title
Biol Reprod
Volume: 88
Issue: 2
Pages: 36-36
DOI
Related Report
Peer Reviewed
-
[Journal Article] Hinge and chromoshadow of HP1α participate in recognition of K9 methylated histone H3 in nucleosomes.2012
Author(s)
Mishima Y, Watanabe M, Kawakami T, Jayasinghe CD, Otani J, Kikugawa Y, Shirakawa M, Kimura H, Nishimura O, Aimoto S, Tajima S, Suetake I.
-
Journal Title
J Mol Biol.
Volume: 425
Issue: 1
Pages: 54-70
DOI
Related Report
Peer Reviewed
-
-
-
[Journal Article] H4K20me1 contributes to downregulation of X-linked genes for C. elegans dosage compensation2012
Author(s)
Vielle A, Lang J, Dong Y, Ercan S, Kotwaliwale C, Rechtsteiner A, Appert A, Chen QB, Dose A, Egelhofer T, Kimura H, Stempor P, Dernburg A, Lieb JD, Strome S, and Ahringer J
-
Journal Title
PLoS Genet
Volume: 8
Issue: 9
Pages: e1002933-e1002933
DOI
Related Report
Peer Reviewed
-
-
[Journal Article] Histone chaperone activity of Fanconi anemia proteins, FANCD2 and FANCI, is required for DNA crosslink repair2012
Author(s)
Koichi Sato. Masamichi Ishiai, Kazue Toda, Satoshi Furukoshi, Akihisa Osakabe, Hiroaki Tachiwana, Yoshimasa Takizawa, Wataru Kagawa, Hiroyuki Kitao, Naoshi Dohmae, Chikashi Obuse, Hiroshi Kimura, Minoru Takata, Hitoshi Kurumizaka
-
Journal Title
The EMBO Journal
Volume: 31
Issue: 17
Pages: 3524-3536
DOI
Related Report
Peer Reviewed
-
[Journal Article] Independence of repressive histone marks and chromatin compaction during senescent heterochromatic layer formation2012
Author(s)
Chandra T, Kirschner K, Thuret JY, Pope BD, Ryba T, Newman S, Ahmed K, Samarajiwa SA, Salama R, Carroll T, Stark R, Janky R, Narita M, Xue L, Chicas A, Nũnez S, Janknecht R, Hayashi-Takanaka Y, Wilson MD, Marshall A, Odom DT, Babu MM, Bazett-Jones DP, Tavaré S, Edwards PA, Lowe SW, Kimura H, Gilbert DM, and Narita M
-
Journal Title
Mol Cell
Volume: 47
Issue: 2
Pages: 203-214
DOI
Related Report
Peer Reviewed
-
-
-
-
-
[Journal Article] Polycomb Associates Genome-wide with a Specific RNA Polymerase II Variant2012
Author(s)
Brookes E, de Santiago I, Hebenstreit D, Morris KJ, Carroll T, Xie SQ, Stock JK, Heidemann M, Eick D, Nozaki N, Kimura H, Ragoussis J, Teichmann SA, and Pombo A
-
Journal Title
and Regulates Metabolic Genes in ESCs. Cell Stem Cell
Volume: 10
Issue: 2
Pages: 157-170
DOI
Related Report
Peer Reviewed
-
[Journal Article] Epigenetic Engineering : Histone H3K9 acetylation is compatible with kinetochore structure and function2012
Author(s)
Bergmann JH, Jakubsche JN, Martins NM, Kagansky A, Nakano M, Kimura H, Kelly DA, Turner BM, Masumoto H, Larionov V, and Earnshaw WC
-
Journal Title
J Cell Sci
Volume: 125
Issue: 2
Pages: 411-421
DOI
Related Report
Peer Reviewed
-
-
-
[Journal Article] Arabidopsis HDA6 regulates locus-directed heterochromatin silencing in cooperation with MET12011
Author(s)
To TK, Kim JM, Matsui A, Kurihara Y, Morosawa T, Ishida J, Tanaka M, Endo T, Kakutani T, Toyoda T, Kimura H, Yokoyama S, Shinozaki K, Seki M
-
Journal Title
PLoS Genetics
Volume: 7
Issue: 4
Pages: e1002055-e1002055
DOI
NAID
Related Report
Peer Reviewed
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-