Live cell imaging of histone methylation and inactive X chromosome dynamics

• Project/Area Number: 22370063
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Molecular biology
• Research Institution: Osaka University
• Principal Investigator: KIMURA Hiroshi 大阪大学, 生命機能研究科, 准教授 (30241392)
• Project Period (FY): 2010-04-01 – 2013-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥18,590,000 (Direct Cost: ¥14,300,000、Indirect Cost: ¥4,290,000); Fiscal Year 2012: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000); Fiscal Year 2011: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000); Fiscal Year 2010: ¥6,110,000 (Direct Cost: ¥4,700,000、Indirect Cost: ¥1,410,000)
• Keywords: 染色体動態 / エピジェネティクス制御 / クロマチン / ヒストン修飾 / 生細胞イメージング / 生体イメージング / 転写制御 / 染色体 / モノクローナル抗体 / エピジェネティクス / ヒストンアセチル化 / 生細胞計測 / ヒストン / 翻訳後修飾 / 遺伝子発現 / 不活性X染色体

Research Abstract

In order to visualize dynamics of inactive X chromosome, we developed two methods monitoring histone modifications in living cells. The first method uses fluorescently labeled antigen-binding fragment (Fab), which binds to endogenous modifications when loaded into living cells. Using this technique, we were able to track the behavior of inactive X chromosomes, where trimethylated histone H3K27me3 is enriched, during the cell cycle. The second method is a genetically encoded system using a single-chain variable fragment tagged with the green fluorescent protein. These methods will be useful to track epigenetic changes during development and differentiation.

Research Products

• [Journal Article] Genetically encoded system to track histone modification in vivo. (2013)
  • Author(s): Yuko Sato, Masanori Mukai, Jun Ueda, Michiko Muraki, Timothy J. Stasevich, Naoki Horikoshi, Tomoya Kujirai, Hiroaki Kita, Taisuke Kimura, Seiji Hira, Yasushi Okada, Yoko Hayashi-Takanaka, Chikashi Obuse, Hitoshi Kurumizaka, Atsuo Kawahara, Kazuo Yamagata, Naohito Nozaki and Hiroshi Kimura
  • Journal Title: Scientific Reports Volume: 3 Issue: 1 Pages: 2436-2436
  • DOI: 10.1038/srep02436
  • Peer Reviewed / Open Access
• [Journal Article] Histone modification for human epigenome analysis (2013)
  • Author(s): Kimura H
  • Journal Title: J Hum Genet Volume: 58 Issue: 7 Pages: 439-445
  • DOI: 10.1038/jhg.2013.66
  • NAID: 10031190039
  • Peer Reviewed
• [Journal Article] Chromosome engineering allows the efficient isolation of vertebrate neocentromeres (2013)
  • Author(s): Shang WH, Hori T, Martins NM, Toyoda A, Misu S, Monma N, Hiratani I, Maeshima K, Ikeo K, Fujiyama A, Kimura H, Earnshaw WC, and Fukagawa T
  • Journal Title: Dev Cell Volume: 24 Issue: 6 Pages: 635-648
  • DOI: 10.1016/j.devcel.2013.02.009
  • Peer Reviewed
• [Journal Article] Human inactive X chromosome is compacted through a PRC2-independent SMCHD1-HBiX1 pathway (2013)
  • Author(s): Nozawa RS, Nagao K, Igami K, Shibata S, Shirai N, Nozaki N, Sado T, Kimura H, and Obuse C
  • Journal Title: Nat. Struct. Mol. Biol Volume: 20 Issue: 5 Pages: 566-573
  • DOI: 10.1038/nsmb.2532
  • Peer Reviewed
• [Journal Article] Vertebrate Spt2 is a novel nucleolar histone chaperone that assists in ribosomal DNA transcription (2013)
  • Author(s): Osakabe A, Tachiwana H, Takaku M, Hori T, Obuse C, Kimura H, Fukagawa T, Kurumizaka H
  • Journal Title: J. Cell Sci Volume: 126 Pages: 1323-1332
  • DOI: 10.1242/jcs.112623
  • Peer Reviewed
• [Journal Article] Nap1 regulates proper CENP-B binding to nucleosomes (2013)
  • Author(s): Tachiwana H, Miya Y, Shono N, Ohzeki J, Osakabe A, Otake K, Larionov V, Earnshaw WC, Kimura H, Masumoto H, Kurumizaka H
  • Journal Title: Nucleic Acids Res Volume: 5 Issue: 5 Pages: 2869-2880
  • DOI: 10.1093/nar/gks1464
  • Peer Reviewed
• [Journal Article] Predominant expression of H3K9 methyltransferases in prehypertrophic and hypertrophic chondrocytes during mouse growth plate cartilage development (2013)
  • Author(s): Ideno H, Shimada A, Imaizumi K, Kimura H, Abe M, Nakashima K, and Nifuji A
  • Journal Title: Gene Expr Pat Volume: 13 Issue: 3-4 Pages: 84-90
  • DOI: 10.1016/j.gep.2013.01.002
  • Peer Reviewed
• [Journal Article] Posttranscriptional Regulation of Histone Lysine Methyltransferase GLP in Embryonic Male Mouse Germ Cells. (2013)
  • Author(s): Deguchi K, Nagamatsu G, Miyachi H, Kato Y, Morita S, Kimura H, Kitano S, Hatada I, Saga Y, Tachibana M, Shinkai Y.
  • Journal Title: Biol Reprod Volume: 88 Issue: 2 Pages: 36-36
  • DOI: 10.1095/biolreprod.112.103572
  • Peer Reviewed
• [Journal Article] Hinge and chromoshadow of HP1α participate in recognition of K9 methylated histone H3 in nucleosomes. (2012)
  • Author(s): Mishima Y, Watanabe M, Kawakami T, Jayasinghe CD, Otani J, Kikugawa Y, Shirakawa M, Kimura H, Nishimura O, Aimoto S, Tajima S, Suetake I.
  • Journal Title: J Mol Biol. Volume: 425 Issue: 1 Pages: 54-70
  • DOI: 10.1016/j.jmb.2012.10.018
  • Peer Reviewed
• [Journal Article] Reliable detection of epigenetic histone marks and nuclear proteins in tissue cryosections. (2012)
  • Author(s): Eberhart A
  • Journal Title: Chromosome Res Volume: 20 Issue: 7 Pages: 849-858
  • DOI: 10.1007/s10577-012-9318-8
  • Peer Reviewed
• [Journal Article] Chromosome-wide regulation of euchromatin-specific 5mC to 5hmC conversion in mouse ES cells and female human somatic cells. (2012)
  • Author(s): Kubiura M
  • Journal Title: Chromosome Res Volume: 20 Issue: 7 Pages: 837-848
  • DOI: 10.1007/s10577-012-9317-9
  • Peer Reviewed
• [Journal Article] H4K20me1 contributes to downregulation of X-linked genes for C. elegans dosage compensation (2012)
  • Author(s): Vielle A, Lang J, Dong Y, Ercan S, Kotwaliwale C, Rechtsteiner A, Appert A, Chen QB, Dose A, Egelhofer T, Kimura H, Stempor P, Dernburg A, Lieb JD, Strome S, and Ahringer J
  • Journal Title: PLoS Genet Volume: 8 Issue: 9 Pages: e1002933-e1002933
  • DOI: 10.1371/journal.pgen.1002933
  • Peer Reviewed
• [Journal Article] H3K9 and H3K14 acetylation co-occur at many gene regulatory elements, while H3K14ac marks a subset of inactive inducible promoters in mouse embryonic stem cells (2012)
  • Author(s): Karmodiya K, Krebs AR, Oulad-Abdelghani M, Kimura H, and Tora L
  • Journal Title: BMC Genomics Volume: 13 Issue: 1 Pages: 424-424
  • DOI: 10.1186/1471-2164-13-424
  • Peer Reviewed
• [Journal Article] Histone chaperone activity of Fanconi anemia proteins, FANCD2 and FANCI, is required for DNA crosslink repair (2012)
  • Author(s): Koichi Sato. Masamichi Ishiai, Kazue Toda, Satoshi Furukoshi, Akihisa Osakabe, Hiroaki Tachiwana, Yoshimasa Takizawa, Wataru Kagawa, Hiroyuki Kitao, Naoshi Dohmae, Chikashi Obuse, Hiroshi Kimura, Minoru Takata, Hitoshi Kurumizaka
  • Journal Title: The EMBO Journal Volume: 31 Issue: 17 Pages: 3524-3536
  • DOI: 10.1038/emboj.2012.197
  • Peer Reviewed
• [Journal Article] Independence of repressive histone marks and chromatin compaction during senescent heterochromatic layer formation (2012)
  • Author(s): Chandra T, Kirschner K, Thuret JY, Pope BD, Ryba T, Newman S, Ahmed K, Samarajiwa SA, Salama R, Carroll T, Stark R, Janky R, Narita M, Xue L, Chicas A, Nũnez S, Janknecht R, Hayashi-Takanaka Y, Wilson MD, Marshall A, Odom DT, Babu MM, Bazett-Jones DP, Tavaré S, Edwards PA, Lowe SW, Kimura H, Gilbert DM, and Narita M
  • Journal Title: Mol Cell Volume: 47 Issue: 2 Pages: 203-214
  • DOI: 10.1016/j.molcel.2012.06.010
  • Peer Reviewed
• [Journal Article] Breaking the HAC Barrier : Histone H3K9 acetyl/ methyl balance regulates CENP-A assembly (2012)
  • Author(s): Ohzeki J-i, Bergmann JH, Kouprina N, Noskov VN, Nakano M, Kimura H, Earnshaw WC, Larionov V, and Masumoto H
  • Journal Title: EMBO J Volume: (in press) Issue: 10 Pages: 2391-2402
  • DOI: 10.1038/emboj.2012.82
  • Peer Reviewed
• [Journal Article] Roles of histone H3K9 methyltransferases during Drosophila spermatogenesis (2012)
  • Author(s): Ushijima Y, Inoue YH, Konishi T, Kitazawa D, Yoshida H, Shimaji K, Kimura H, Yamaguchi M
  • Journal Title: Chromosome Research Volume: 3 Issue: 3 Pages: 319-31
  • DOI: 10.1007/s10577-012-9276-1
  • Peer Reviewed
• [Journal Article] Lamin B receptor recognizes specific modifications of histone H4 in heterochromatin formation (2012)
  • Author(s): Hirano Y, Hizume K, Kimura H, Takeyasu K, Haraguchi T, Hiraoka Y
  • Journal Title: J. Biol. Chem Volume: 287 Issue: 51 Pages: 42654-42663
  • DOI: 10.1074/jbc.m112.397950
  • Peer Reviewed
• [Journal Article] Monitoring Post-translational Modification on Endogenous Proteins in Living Cells (2012)
  • Author(s): 木村 宏
  • Journal Title: Seibutsu Butsuri Volume: 52 Issue: 5 Pages: 234-235
  • DOI: 10.2142/biophys.52.234
  • NAID: 10030877024
  • ISSN: 0582-4052, 1347-4219
  • Peer Reviewed
• [Journal Article] Polycomb Associates Genome-wide with a Specific RNA Polymerase II Variant (2012)
  • Author(s): Brookes E, de Santiago I, Hebenstreit D, Morris KJ, Carroll T, Xie SQ, Stock JK, Heidemann M, Eick D, Nozaki N, Kimura H, Ragoussis J, Teichmann SA, and Pombo A
  • Journal Title: and Regulates Metabolic Genes in ESCs. Cell Stem Cell Volume: 10 Issue: 2 Pages: 157-170
  • DOI: 10.1016/j.stem.2011.12.017
  • Peer Reviewed
• [Journal Article] Epigenetic Engineering : Histone H3K9 acetylation is compatible with kinetochore structure and function (2012)
  • Author(s): Bergmann JH, Jakubsche JN, Martins NM, Kagansky A, Nakano M, Kimura H, Kelly DA, Turner BM, Masumoto H, Larionov V, and Earnshaw WC
  • Journal Title: J Cell Sci Volume: 125 Issue: 2 Pages: 411-421
  • DOI: 10.1242/jcs.090639
  • Peer Reviewed
• [Journal Article] Fluorescence cell imaging and manipulation using conventional halogen lamp microscopy (2011)
  • Author(s): Yamagata K
  • Journal Title: PLoS One Volume: 7 Issue: 2 Pages: e31638-e31638
  • DOI: 10.1371/journal.pone.0031638
  • Peer Reviewed
• [Journal Article] Tracking epigenetic histone modifications in single cells using Fab-based live endogenous modification labeling (2011)
  • Author(s): Hayashi-Takanaka Y
  • Journal Title: Nucleic Acids Res Volume: 39 Issue: 15 Pages: 6475-6488
  • DOI: 10.1093/nar/gkr343
  • Peer Reviewed
• [Journal Article] Arabidopsis HDA6 regulates locus-directed heterochromatin silencing in cooperation with MET1 (2011)
  • Author(s): To TK, Kim JM, Matsui A, Kurihara Y, Morosawa T, Ishida J, Tanaka M, Endo T, Kakutani T, Toyoda T, Kimura H, Yokoyama S, Shinozaki K, Seki M
  • Journal Title: PLoS Genetics Volume: 7 Issue: 4 Pages: e1002055-e1002055
  • DOI: 10.1371/journal.pgen.1002055
  • NAID: 130006996644
  • Peer Reviewed
• [Journal Article] Broad chromosomal domains of histone modification patterns in C.elegans (2011)
  • Author(s): Liu, T.
  • Journal Title: Genome Res. Volume: 21 Pages: 227-236
  • Peer Reviewed
• [Journal Article] Epigenetic engineering shows H3K4me2 is required for HJURP targeting and CENP-A assembly on a synthetic human kinetochore (2011)
  • Author(s): Bergmann, J.H.
  • Journal Title: EMBO J. Volume: 30 Pages: 328-340
  • Peer Reviewed
• [Journal Article] Autocatalytic differentiation of epigenetic modifications within the Arabidopsis genome. (2010)
  • Author(s): Inagaki, S.
  • Journal Title: EMBO J. Volume: 29 Pages: 3496-3506
  • Peer Reviewed
• [Journal Article] The histone H3K36 methyltransferase MES-4 acts epigenetically to transmit the memory of germline gene expression to progeny. (2010)
  • Author(s): Rechtsteiner, A.
  • Journal Title: PLoS Genet. Volume: 6
  • Peer Reviewed
• [Journal Article] Oncogenesis by sequestration of CBP/p300 in transcriptionally inactive hyperacetylated chromatin domains (2010)
  • Author(s): Reynoird, N.
  • Journal Title: EMBO J. Volume: 29 Pages: 2943-2952
  • Peer Reviewed
• [Journal Article] Trans-generational epigenetic regulation of C.elegans primordial germ cells (2010)
  • Author(s): Furuhashi, H.
  • Journal Title: Epigenetics Chromatin Volume: 3 Pages: 15-15
  • Peer Reviewed
• [Journal Article] エピゲノム制御にかかわるヒストン修飾のイメージング (2010)
  • Author(s): 木村宏
  • Journal Title: 医学のあゆみ Volume: 235 Pages: 995-1000
• [Journal Article] 特異的モノクローナル抗体を用いたメチル化ヒストンダイナミクスの可視化 (2010)
  • Author(s): 木村宏
  • Journal Title: 実験医学増刊 Volume: 28 Pages: 115-121
• [Presentation] 1. Monitoring the dynamics of transcription and epigenome modifications in living cells and organisms. (2013)
  • Author(s): Hiroshi Kimura
  • Organizer: 1st International Symposium of the Mathematics on Chromatin Live Dynamics
  • Place of Presentation: 広島
  • Invited
• [Presentation] Dynamic regulation of epigenetic modifications in living cells. (2012)
  • Author(s): Hiroshi Kimura
  • Organizer: The 2nd International Workshop on Structural Epigenomics
  • Place of Presentation: Yokohama, Japan
  • Year and Date: 2012-10-10
  • Invited
• [Presentation] ヒストン翻訳後修飾の生細胞動態可視化プローブの開発 (2012)
  • Author(s): 木村宏
  • Organizer: 第85回日本生化学会大会・シンポジウム「ヒストンの多様性とクロマチン機能制御機構」
  • Place of Presentation: 福岡
  • Invited
• [Presentation] ヒストン修飾動態制御の単一細胞解析 (2012)
  • Author(s): 木村宏
  • Organizer: 第35回日本分子生物学会年会ワークショップ「細胞核構造体によるエピゲノム制御機構」
  • Place of Presentation: 福岡
  • Invited
• [Presentation] ヒストン翻訳後修飾の生細胞動態可視化プローブの開発． (2012)
  • Author(s): 木村 宏
  • Organizer: 第85回日本生化学会大会
  • Place of Presentation: 福岡
  • Invited
• [Presentation] ヒストン修飾動態制御の単一細胞解析． (2012)
  • Author(s): 木村 宏
  • Organizer: 第35回日本分子生物学会年会
  • Place of Presentation: 福岡
  • Invited
• [Presentation] 『Opto-epigenetics（オプトエビジェネティクス）』の創生へ向けて (2012)
  • Author(s): 木村 宏
  • Organizer: 第7回NIBBバイオイメージングフォーラム －顕微鏡の新機軸－
  • Place of Presentation: 岡崎
  • Invited
• [Presentation] Dynamic regulation of epigenetic modifications in living cells. (2012)
  • Author(s): Hiroshi Kimura
  • Organizer: The 2nd International Workshop on Structural Epigenomics
  • Place of Presentation: 横浜
  • Invited
• [Presentation] Monitoring epigenetic modifications in living cells (2011)
  • Author(s): Hiroshi Kimura
  • Organizer: 第34回日本分子生物学会大会
  • Place of Presentation: パシフィコ横浜(横浜市)(招待講演)
  • Year and Date: 2011-12-16
• [Presentation] ヒストンH3のメチル化とアセチル化の生細胞ダイナミクス (2011)
  • Author(s): 木村宏
  • Organizer: 第84回日本生化学会大会
  • Place of Presentation: 京都国際会館(京都市)(招待講演)
  • Year and Date: 2011-09-22
• [Presentation] Endogenous histone H3 lysine modifications : high H3K27 acetylation is important for normal embryo development (2011)
  • Author(s): Hiroshi Kimura
  • Organizer: 18^<th> International Chromosome Conference
  • Place of Presentation: Manchester University (Manchester、イギリス)
  • Year and Date: 2011-09-02
• [Presentation] 蛋白質翻訳後修飾の生細胞イメージング (2011)
  • Author(s): 木村宏
  • Organizer: 第11回蛋白質科学会年会
  • Place of Presentation: ボテル阪急エキスポパーク(大阪府吹田市)(招待講演)
  • Year and Date: 2011-06-08
• [Presentation] FabLEM (Fab-based live endogenous modification labeling)を用いたヒストンH3のメチル化とアセチル化の生細胞ダイナミクス (2011)
  • Author(s): 木村宏
  • Organizer: 第5回日本エピジェネティクス研究会
  • Place of Presentation: KKRホテル熊本(熊本市)
  • Year and Date: 2011-05-19
• [Presentation] Small molecule screening for epigenetic modulators (2011)
  • Author(s): Hiroshi Kimura
  • Organizer: UK/Japan Collaborative Symposium : Interdisciplinary approaches for the study of senescence
  • Place of Presentation: CRUK-CRI (Cambridge、イギリス)(招待講演)
  • Year and Date: 2011-02-09
• [Presentation] Small molecule screening for epigenetic modulators (2011)
  • Author(s): Hiroshi Kimura.
  • Organizer: JSPS UK/Japan Collaborative Symposium"Interdisciplinary approaches for the study of senescence"
  • Place of Presentation: Cambridge, UK
  • Invited
• [Presentation] Monitoring epigenetic modifications in living cells (2011)
  • Author(s): Hiroshi Kimura
  • Organizer: 第34回日本分子生物学会年会ワークショップ「染色体における遺伝情報の発現と維持のメカニズム」
  • Place of Presentation: 横浜
  • Invited
• [Presentation] ヒストンH3のメチル化とアセチル化の生細胞ダイナミクス (2011)
  • Author(s): 木村宏
  • Organizer: 第84回日本生化学会大会シンポジウム「クロマチンダイナミクスによるエピジェネティクスの分子機構」
  • Place of Presentation: 京都
  • Invited
• [Presentation] 蛋白質翻訳後修飾の生細胞イメージング (2011)
  • Author(s): 木村宏
  • Organizer: 第11回日本蛋白質科学会年会ワークショップ「タンパク質修飾のイメージング」
  • Place of Presentation: 吹田
  • Invited
• [Presentation] Visualizing histone modification dynamics in living cells (2011)
  • Author(s): Hiroshi Kimura
  • Organizer: International Symposium on the Physicochemical Field for Genetic Activities
  • Place of Presentation: Awaji, Japan
  • Invited
• [Presentation] Visualization of epigenetic histone modifications in living cells (2010)
  • Author(s): Hiroshi Kimura
  • Organizer: JSPS Sweden-Japan Joint Colloquium"Epigenetics -new horizons in Japan and Scandinavia-"
  • Place of Presentation: Stockholm, Sweden
  • Year and Date: 2010-09-06
  • Invited
• [Presentation] Visualizing histone modification dynamics in living cells (2010)
  • Author(s): Hiroshi Kimura
  • Organizer: JSPS Sweden-Japan Joint Colloquim"Epigenetics-new horizons in Japan and Scandinavia"
  • Place of Presentation: ストックホルム(スウェーデン)(招待講演)
  • Year and Date: 2010-09-06
• [Presentation] ヒストン修飾と高次クロマチン構造のダイナミクス (2010)
  • Author(s): 木村宏
  • Organizer: 構造エピゲノム研究会発足記念シンポジウム
  • Place of Presentation: 横浜(招待講演)
  • Year and Date: 2010-04-30
• [Presentation] Live cell imaging of epigenetic histone modifications (2010)
  • Author(s): Hiroshi Kimura
  • Organizer: BMB2010・シンポジウム
  • Place of Presentation: 神戸
  • Invited
• [Presentation] エピジェネティック制御に関わるヒストン修飾の生細胞イメージング (2010)
  • Author(s): 木村宏
  • Organizer: 日本遺伝学会第82回大会シンポジウム「多様な生命現象におけるエピゲノム制御」
  • Place of Presentation: 札幌
  • Invited
• [Presentation] Live cell imaging of epigenetic histone modifications (2010)
  • Author(s): Hiroshi Kimura
  • Organizer: BMB2010シンポジウム「エピゲノム情報のリードアウトと制御」
  • Place of Presentation: 神戸(招待講演)
• [Presentation] エピジェネティック制御に関わるヒストン修飾の生細胞イメージング (2010)
  • Author(s): 木村宏
  • Organizer: 日本遺伝学会第82回大会シンポジウム「多様な生命現象におけるエピゲノム制御」
  • Place of Presentation: 札幌(招待講演)
• [Presentation] 細胞周期におけるヒストン修飾のダイナミクス (2010)
  • Author(s): 木村宏
  • Organizer: 日本エピジェネティクス研究会第4回大会
  • Place of Presentation: 米子
• [Presentation] Subnuclear distributions of histone H3 and H4 modifications as revealed by a panel of modification specific antibodies (2010)
  • Author(s): Hiroshi Kimura
  • Organizer: 第62回日本細胞生物学会大会
  • Place of Presentation: 大阪
• [Book] エピジェネティクス―基礎研究から産業応用への展望―（畑田出穂、久保田健夫／編） (2014)
  • Author(s): 佐藤優子、木村 宏
  • Total Pages: 393
  • Publisher: シーエムシー出版
• [Book] 染色体と細胞核のダイナミクス（平岡 泰・原口徳子／編） (2013)
  • Author(s): 木村 宏、胡桃坂仁志
  • Total Pages: 236
  • Publisher: 化学同人
• [Book] エピジェネティクスキーワード事典（牛島俊和・眞貝洋一／編） (2013)
  • Author(s): 木村 宏
  • Total Pages: 315
  • Publisher: 羊土社
• [Book] 遺伝子医学MOOK 25号「エピジェネティクスと病気」（佐々木裕之／監、中尾光善、中島欽一／編） (2013)
  • Author(s): 木村 宏, 佐藤優子
  • Total Pages: 280
  • Publisher: メディカル・ドゥ
• [Book] 特異的モノクローナル抗体を用いたメチル化ヒストンダイナミクスの可視化,エピジェネティクスと疾患(牛島俊和,塩田邦郎,田嶋正二,吉田稔/編)(実験医学増刊) (2010)
  • Author(s): 木村宏,林陽子
  • Publisher: 羊土社
• [Remarks]
  • URL: http://www.fbs.osaka-u.ac.jp/labs/hiraoka/kimura/index.html
• [Remarks]
  • URL: http://www.dma.jim.osaka-u.ac.jp/view?l=ja&u=7721
• [Remarks] 木村グループHP
  • URL: http://www.fbs.osaka-u.ac.jp/labs/hiraoka/kimura/index.html
• [Remarks] 大阪大学生命機能研究科教員基本情報 木村 宏
  • URL: http://www.dma.jim.osaka-u.ac.jp/view?l=ja&u=7721
• [Remarks] Google Scholar Hiroshi Kimura
  • URL: http://scholar.google.co.jp/citations?user=z3cSm10AAAAJ&hl=ja&oi=ao
• [Remarks]
  • URL: http://www.dma.jim.osaka-u.ac.jp/kg-portal/aspi/RX001D.asp?UN0=17710&page=
• [Remarks]
  • URL: http://www.fbs.osaka-u.ac.jp/labs/hiraoka/index.html
• [Remarks]
  • URL: http://www.dma.jim.osaka-u.ac.jp/kg-portal/aspi/RX0011D.asp?UNO=17710&page=