Molecular analyses on the replication initiation complex and its novel regulatory factors in E. coli
| Project/Area Number |
22370064
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Molecular biology
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| Research Institution | Kyushu University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
UEDA Tadashi 九州大学, 大学院・薬学研究院, 教授 (90184928)
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| Project Period (FY) |
2010 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥18,850,000 (Direct Cost: ¥14,500,000、Indirect Cost: ¥4,350,000)
Fiscal Year 2012: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2011: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000)
Fiscal Year 2010: ¥7,150,000 (Direct Cost: ¥5,500,000、Indirect Cost: ¥1,650,000)
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| Keywords | DnaA / DnaB / oriC / ATP / 細胞周期制御 / 染色体複製開始制御 / タンパク質高次複合体 / 試験管内再構成 / 複製開始複合体 / へリカーゼ装着 / 複製開始の制御機構 / 非コードDNA / 核酸タンパク質複合体 |
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| Research Abstract |
The initiation of the chromosomal DNA replication requires formation of higher-order nucleoprotein complexes and their functions must be regulated during the cell cycle. In this study, by analyzing higher-order complexes formed by the replication origin DNA and the replication initiator DnaA protein in E. coli as a model cell, we identified subcomplexes important for replication initiation reactions and revealed novel molecular mechanisms. Moreover, by analyzing major factors regulating DnaA functions, we revealed a novel regulatory mechanism for DnaA in addition to novel interaction modes between DnaA and the factors
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Report
(4 results)
Research Products
(100 results)
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[Journal Article] The DnaA N-terminal domain interacts with Hda to facilitate replicase clamp-mediated inactivation of DnaA2013
Author(s)
Su'etsugu, M., Harada, Y., Keyamura, K., Matsunaga, C., Kasho, K., Abe, Y., Ueda, T. and Katayama, T.
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Journal Title
nviron. Microbiol.
Volume: (印刷中)
Issue: 12
Pages: 3183-3195
DOI
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Peer Reviewed
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[Presentation] (未定）2013
Author(s)
片山 勉
Organizer
第86回日本生化学会大会 （シンポジウム）
Place of Presentation
横浜市
Related Report
Invited
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[Presentation] DnaA, the replication initiator, is activated for the timely initiation of the chromosomal replication by a specific DNA element, DARS2011
Author(s)
Fujimitsu, K., Kasho, K., Ozaki, S., Keyamura, K. and Katayama, T.
Organizer
International Symposium on the Physicochemical Field for Genetic Activities
Place of Presentation
Awaji
Related Report
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[Presentation] DARS, DnaA-reactivating sequence promotes exchange of DnaA-bound ADP to ATP.2010
Author(s)
Fujimitsu, K., Kasho, K., Ozaki, S., Senriuchi, T. and Katayama, T.
Organizer
EMBO Conference Series; Replication, Repair & Segregation of Chromosomes
Place of Presentation
Germany
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