| Budget Amount *help |
¥18,850,000 (Direct Cost: ¥14,500,000、Indirect Cost: ¥4,350,000)
Fiscal Year 2012: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2011: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000)
Fiscal Year 2010: ¥7,150,000 (Direct Cost: ¥5,500,000、Indirect Cost: ¥1,650,000)
|
|---|
| Research Abstract |
The initiation of the chromosomal DNA replication requires formation of higher-order nucleoprotein complexes and their functions must be regulated during the cell cycle. In this study, by analyzing higher-order complexes formed by the replication origin DNA and the replication initiator DnaA protein in E. coli as a model cell, we identified subcomplexes important for replication initiation reactions and revealed novel molecular mechanisms. Moreover, by analyzing major factors regulating DnaA functions, we revealed a novel regulatory mechanism for DnaA in addition to novel interaction modes between DnaA and the factors
|
