Integrin dependent cellular growth and functions through the mammalianhippo pathway

• Project/Area Number: 22370072
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Cell biology
• Research Institution: Kansai Medical University
• Principal Investigator: KINASHI Tatsuo 関西医科大学, 医学部, 教授 (30202039)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥19,110,000 (Direct Cost: ¥14,700,000、Indirect Cost: ¥4,410,000); Fiscal Year 2012: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000); Fiscal Year 2011: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000); Fiscal Year 2010: ¥8,190,000 (Direct Cost: ¥6,300,000、Indirect Cost: ¥1,890,000)
• Keywords: Mst1,Mst2,インテグリン / Outside-in シグナル / Rap1 / Mst1 / Mat2 / インテグリン / Mst2 / Outside-inシグナル

Research Abstract

Mst1/Mst2, Mammalian homolog of Hippo associates with Rap1 effector RAPL and regulates lymphocyte adhesion and migration through leukocyte integrin LFA-1. Furthermore, RAPL negatively regulate cell cycle through G1 cyclin inhibitor p27Kip1. In addition, tissue imaging analysis has revealed that Mst1 is required for efficient thymocyte trafficking and selection. Consequently, deficiencies of RAPL and Mst1 lead to lymphoproliferative and autoimmune-like disorders.

Research Products

• [Journal Article] Mst1 regulates integrin-dependent thymocyte trafficking and antigen-recognition in the thymus. (2012)
  • Author(s): Ueda Y., Katagiri K., Tomiyama T., Yasuda K., Habiro K., Katakai T., Ikehara S., Matsumoto M, Kinashi T.
  • Journal Title: Nat. Commun. Volume: 3 Issue: 1 Pages: 1098-1098
  • DOI: 10.1038/ncomms2105
  • Peer Reviewed
• [Journal Article] Reelin controls neuronal positioning by promoting cell-matrix adhesion via inside-out activation of integrin α5β 1 (2012)
  • Author(s): Katsutoshi Sekine, Takeshi Kawauchi, Ken-ichiro Kubo, Takao Honda, Joachim Herz, Mitsuharu Hattori, Tatsuo Kinashi, and Kazunori Nakajima
  • Journal Title: Neuron Volume: 76(2) Issue: 2 Pages: 353-369
  • DOI: 10.1016/j.neuron.2012.07.020
  • Peer Reviewed
• [Journal Article] DOCK8 is a Cdc42 activator critical for interstitial dendritic cell migrataon during immune responses (2012)
  • Author(s): Yosuke Harada, Yoshihiko Tanaka, Masao Terasawa, Markus Pieczyk, Katsuyoshi Habiro, Tomoya Katakai, Kyoko Hanawa-Suetsugu, Mutsuko Kukimoto-Niino, Tomoko Nishizaki, Mikako Shirouzu, Xuefeng Duan, Takehito Uruno, Akihiko Nishikimi, Fumiyuki Sanematsu, Shigeyuki Yokoyama, Jens V.Stein, Tatsuo Kinashi, Yoshinori Fukui
  • Journal Title: Blood Volume: (In press) Issue: 19 Pages: 4451-4461
  • DOI: 10.1182/blood-2012-01-407098
  • Peer Reviewed
• [Journal Article] Overview of integrin signaling in the immune system. (2012)
  • Author(s): Kinashi, T.
  • Journal Title: Methods Mol. Biol. Volume: 757 Pages: 261-78
  • DOI: 10.1007/978-1-61779-166-6_17
  • ISBN: 9781617791659, 9781617791666
  • Peer Reviewed
• [Journal Article] Surf4 modulates STIM1-dependent calcium entry. (2012)
  • Author(s): Fujii, Y. et al
  • Journal Title: Biochem. Biophys. Res. Commun. Volume: 422 Issue: 4 Pages: 615-620
  • DOI: 10.1016/j.bbrc.2012.05.037
  • Peer Reviewed
• [Journal Article] Overview of integrin signaling in the immune system (2012)
  • Author(s): Kinashi, T.
  • Journal Title: Methods Mol.Biol. Volume: 757 Pages: 261-78
  • Peer Reviewed
• [Journal Article] RAPL deficiency causedlymphoproliferative disordersthrough the mislocalization of p27kip. (2011)
  • Author(s): Katagiri, K., Ueda, Y., Tomiyama, T., Yasuda, K., Toda, Y., Ikehara, S., Nakayama, K.I, Kinashi, T.
  • Journal Title: Immunity. Volume: 34 Pages: 1-15
  • Peer Reviewed
• [Journal Article] RAPL deficiency caused lymphoproliferative disorders through the mislocalization of p27^<kip> (2011)
  • Author(s): Katagiri, K., Ueda, Y., Tomiyama, T., Yasuda, K., Toda, Y., Ikehara, S., Nakayama, K.I, Kinashi, T.
  • Journal Title: Immunity Volume: 34 Pages: 24-38
  • Peer Reviewed
• [Presentation] Regulation of Lymphocyte "Stop and Go": Analysis of Lymphocyte Trafficking using Live Imaging Techniques (2013)
  • Author(s): Kinashi T., Katakai T.,Ueda Y., Kondo N..
  • Organizer: 第23回CDBミーティング"Building multicellular systems from cellular cross-talk
  • Place of Presentation: 神戸
  • Year and Date: 2013-01-22
• [Presentation] 関西医科大学附属生命医学研究所分子遺伝学部門免疫細胞の組織内移動と停止の制御 (2012)
  • Author(s): 木梨達雄、片貝智哉、植田祥啓、羽廣克嘉
  • Organizer: イメージング手法による接着制御の解析バイオイメージング学会
  • Place of Presentation: 京都国際会館
  • Year and Date: 2012-08-28
• [Presentation] Mst1 regulates integrin-dependent thymocyte trafficking and antigen recognition in the thymus. (2012)
  • Author(s): KINASHI Tatsuo
  • Organizer: 第41回日本免疫学会学術集会シンポジウム
  • Place of Presentation: 神戸
• [Presentation] Mst1 regulates integrin-dependent thymocyte trafficking and antigen recognition in the thymus. (2012)
  • Author(s): Tatsuo Kinashi
  • Organizer: 第41回日本免疫学会学術集会シンポジウム
  • Place of Presentation: 神戸国際会議場
• [Presentation] Crucial roles of Mst1 and RAPL (RASSF5b) in lymphocyte adhesion and proliferation (2011)
  • Author(s): Kinashi T., Katagiri K., Katakai T., Ueda Y., Habiro K.
  • Organizer: The second workshop on the HIPPO tumor suppressor pathway
  • Place of Presentation: Rome, Italy
  • Year and Date: 2011-11-25
• [Presentation] イメージングによるリンパ球組織内移動の調節とその破綻の解析 (2011)
  • Author(s): 木梨達雄
  • Organizer: 第61回日本アレルギー学会秋季学術大会
  • Place of Presentation: グランドプリンスホテル・東京
  • Year and Date: 2011-11-12
• [Presentation] Mst1 regulates thymocyte trafficking and antigen recognition within thymic tissues. (2011)
  • Author(s): Ueda Y., Katagiri K., Tomiyama T., Yasuda K., Habiro K., Katakai T., Ikehara S., Kinashi T..
  • Organizer: 2011 The Annual Meeting of the Japanese Society for Immunology
  • Place of Presentation: Chiba Japan
• [Presentation] Crucial roles of Mst1 and RAPL (RASSF5) in lymphocyte adhesion and proliferation. (2011)
  • Author(s): Kinashi T., Katagiri K., Katakai T., Ueda Y., Habiro K..
  • Organizer: 2nd International RASSF Symposium
  • Place of Presentation: Oxford UK
• [Presentation] Mst1 regulates thymocyte trafficking and antigen recognition within thymic tissues (2011)
  • Author(s): Ueda Yoshihiro1, Katagiri Koko2, Tomiyama Takashi1, Yasuda Kaneki1, Habiro Katsuyoshi1, Katakai Tomoya1, Ikehara Susumu3, Kinashi Tatsuo1, (1Department of Molecular Genetics, Institute of Biomedical Science, Kansai Medical University. 2Department of Bioscience, School of Science and Technology, Kwansei Gakuin University. 3Department of Stem Cell Disorders. Kansai Medical University.)
  • Organizer: 2011 The Annual Meeting of the Japanese Society for Immunology
  • Place of Presentation: Makuhari Messe Chiba Japan
• [Presentation] Crucial roles of Mst1 and RAPL (RASSF5) in lymphocyte adhesion and proliferation (2011)
  • Author(s): Tatsuo Kinashi, Koko Katagiri, Katakai Tomoya, Yoshihiro Ueda, Katsuyoshi Habiro
  • Organizer: 2nd International RASSF Symposium
  • Place of Presentation: Oxford England
• [Presentation] Distinct signaling processes in regulation of lymphocyte arrest and adhesion strengthening under shear flow (2010)
  • Author(s): Kinashi.T.
  • Organizer: The 16th International Vascular Biology Meeting Los
  • Place of Presentation: Angeles,USA
• [Presentation] Crucial roles of Mstl and RAPL (RASSF5b) in lymphocyte adhesion and proliferation (2010)
  • Author(s): Tatsuo Kinashi, Koko Katagiri, Katakai Tomoya, Yoshihiro Ueda, Katsuyoshi Habiro
  • Organizer: The second workshop on the HIPPO tumor suppressor pathway
  • Place of Presentation: Rome, Italy
• [Presentation] Distinct signaling processes in regulation of lymphocyte arrest and adhesi on strengthening under shear flow (2010)
  • Author(s): T.Kinashi, K.Katagiri, T.Katakai, Y.Ueda
  • Organizer: The 16th International Vascular Biology Meeting
  • Place of Presentation: Los Angeles, USA
• [Presentation] Regulation of Lymphocyte “Stop and Go”: Analysis of Lymphocyte Trafficking using Live Imaging Techniques
  • Author(s): Tatsuo Kinashi
  • Organizer: 第23回CDBミーティング”Building multicellular systems from cellular cross-talk”
  • Place of Presentation: 理化学研究所神戸研究所
  • Invited
• [Presentation] 接着制御破綻による自己免疫発症の機序
  • Author(s): 木梨達雄
  • Organizer: 第１６回中国地区小児免疫宅物療法研究会
  • Place of Presentation: リーガロイヤルホテル広島
• [Presentation] 免疫細胞の組織内移動と停止の制御：イメージング手法による接着制御の解析
  • Author(s): 木梨達雄
  • Organizer: バイオイメージング学会
  • Place of Presentation: 国立京都国際会館
• [Book] インテグリンファミリー(LFA-1,VLA-4ICAM-1,VCAM-1)<Seriesモデル動物利用マニュアル>疾患モデルの作成と利用-免疫疾患、岩倉洋一郎編、エル・アイ・シー、1 (2011)
  • Author(s): 木梨達雄
• [Book] <Seriesモデル動物利用マニュアル>疾患モデルの作成と利用-免疫疾患 (2011)
  • Author(s): 木梨達雄(岩倉洋一郎編)
  • Publisher: インテグリンファミリー(LFA-1,VLA-4ICAM-1,VCAM-1)
• [Remarks]
  • URL: http://www3.kmu.ac.jp/molgent/index.html