Evolutionary mechanisms of the gastrula organizer and blastopore formation by focusing on the gene regulatory network
| Project/Area Number |
22370075
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Developmental biology
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| Research Institution | The University of Tokyo |
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Principal Investigator |
TAIRA Masanori 東京大学, 理学(系)研究科(研究院), 准教授 (60150083)
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| Project Period (FY) |
2010-04-01 – 2013-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥18,720,000 (Direct Cost: ¥14,400,000、Indirect Cost: ¥4,320,000)
Fiscal Year 2012: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2011: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2010: ¥8,710,000 (Direct Cost: ¥6,700,000、Indirect Cost: ¥2,010,000)
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| Keywords | クロマチン免疫沈降 / 原腸胚オーガナイザー / ネッタイツメガエル / アフリカツメガエル / ChIP-seq解析 / 転写因子 / ヒストン修飾 / 転写制御 / Lim1/Lhx1 / Otx2 / 発生準備エンハンサー / オーガナイザー / ChIP-seq / Lhx1/Lim1 / p300 / TLE/Groucho |
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| Research Abstract |
In early vertebrate development, the basic body plan is established by the gastrula organizer. To examine the role of several transcription factors that play a central role in this important tissue as well as its evolutionary scenario, we carried out chromatin-immunoprecipitation sequencing (ChIP-seq) analysis using Xenopus gastrula embryos. The data suggest that transcription factors Otx2, Lim1/Lhx1, Gsc, and Sia combinatorially bind to gene regulatory regions, and positively and negatively regulate several hundreds of genes. In addition, comparative functional analysis of regulatory regions between Xenopus and an ancient type of vertebrates, amphioxus, suggest a scenario, in which evolution in activities of regulatory regions of some organizer genes contributed for the vertebrate ancestor to have acquired the head structure.
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Report
(4 results)
Research Products
(42 results)
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[Journal Article] Occupancy of Tissue-Specific cis-Regulatory Modules by Otx2 and TLE/Groucho for Embryonic Head Specification2014
Author(s)
Yasuoka Y., Suzuki Y., Takahashi S., Sudou N., Haramoto Y., Tando Y., Kubokawa K., Cho K. W., Asashima M., Sugano S., and Taira M.
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Journal Title
Nat. Commun
Volume: (in press)
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Peer Reviewed
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[Presentation] Massively parallel regulation of head and non-head genes by Otx2, Lim1 and Gsc is based on evolution of the head organizer in the chordate2012
Author(s)
Yasuoka Y., Suzuki Y., Takahashi S., Sudou N., Haramoto Y., Tando Y., Kubokawa K., Cho K. W., Asashima M., Sugano S., and Taira M.
Organizer
日本発生生物学会第45回大会
Place of Presentation
神戸
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[Presentation] Direct contributions of Otx2 as a positional tag to global gene regulation in the head organizer in Xenopus embryos2011
Author(s)
Yasuoka Y., Suzuki Y., Takahashi S., Sudou N., Haramoto Y., Asashima M., Sugano S., and Taira M.
Organizer
International Symposium"Genetic Regulation of Development"Commemorative of 27th International Prize for Biology, 2011, Celebrating Dr. Eric Davidson
Place of Presentation
京都ガーデンパレス,京都
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