A new model of the self-organization of a complex ordered architecture, spiculous skeleton formation of demosponges

• Project/Area Number: 22370077
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Developmental biology
• Research Institution: Kyoto University
• Principal Investigator: FUNAYAMA Noriko 京都大学, 大学院・理学研究科, 准教授 (30276175)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥19,240,000 (Direct Cost: ¥14,800,000、Indirect Cost: ¥4,440,000); Fiscal Year 2012: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000); Fiscal Year 2011: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2010: ¥10,660,000 (Direct Cost: ¥8,200,000、Indirect Cost: ¥2,460,000)
• Keywords: 器官形成 / 骨片骨格 / カイメン / カワカイメン / シグナル経路 / パターニング / 細胞間相互作用 / 個体形成 / 発生ロジック / 位置情報

Research Abstract

We approached to clarify the mechanisms that regulate the roughly evenly spaced pattern of spicule held up points in the first tier. We succeeded to establish a new original experimental system (sponge fusion experiment) and reviled that the spicules are transported and held up one by one (not simultaneously held up). We selected the inhibitors for several signaling pathways that can be expected to work in Ehydatia.fluviatilis according to the amino acids of their action sites in E.fluviatilis. Using these inhibitors, we succeeded to get candidate signaling path way that affects the pattern of spicule held up points. Identification of genes of this signaling pathway and the examination of the mRNA expression pattern of these genes suggested that the pattern of spicule held up points can not be explained by the simple pre-pattering in basopinaoderm.

Research Products

• [Journal Article] The active stem cell specific expression of sponge Musashi homolog EflMsiA suggests its involvement in maintaining the stem cell state (2012)
  • Author(s): Okamoto K, Nakatsukasa M, Alie A, Masuda Y, Agata A, and Funayama N
  • Journal Title: Mec. Dev Volume: 129 Issue: 1-4 Pages: 24-37
  • DOI: 10.1016/j.mod.2012.03.001
  • Peer Reviewed
• [Journal Article] The active stem cell specific expression of sponge Musashi homolog EflMsiA suggests its involvement in maintaining the stem cell state (2012)
  • Author(s): Okamoto K
  • Journal Title: Mec. Dev. Volume: 129 Pages: 24-37
  • Peer Reviewed
• [Journal Article] The active stem cell specific expression of sponge Musashi homolog EflMsiA suggests its involvement in maintaining the stem cell state (2012)
  • Author(s): Kazuko Okamoto, Mikiko Nakatsukasa, Alexandre Alie, Yoshiki Masuda, Kiyokazu Agata, Noriko Funayama
  • Journal Title: Mechanisms of Development Volume: (e-pub)(in press)(in pressより未決定)
  • Peer Reviewed
• [Presentation] Establishing the first gene functional analysis system in sponge: RNAi method in Ephydatia fluviatilis (2011)
  • Author(s): Kazuko Okamoto, Kiyokazu Agata, Noriko Funayama
  • Organizer: 第44回日本発生生物学会
  • Place of Presentation: 沖縄コンベンションセンター(沖縄県)
  • Year and Date: 2011-05-19
• [Presentation] Investigation of the molecular mechanism that recruit spicule-carrying transport cells to their holding-up points in a freshwater sponge (2011)
  • Author(s): Yudai Nakata, Kiyokazu Agata and Noriko Funayama
  • Organizer: 第44回日本発生生物学会
  • Place of Presentation: 沖縄コンベンションセンター(沖縄県)
  • Year and Date: 2011-05-19
• [Presentation] Investigation of the molecular mechanism that recruit spicule-carrying transport cells to their holding-up points in a freshwater sponge(淡水海綿における骨片運搬細胞の誘導の分子機構解明の試み) (2011)
  • Author(s): 中田裕大・阿形清和・船山典子
  • Organizer: 第44回日本発生生物学会
  • Place of Presentation: 沖縄コンベンションセンター(沖縄県)
  • Year and Date: 2011-05-19
• [Presentation] Establishing the first gene functional analysis system in sponge : RNAi method in Ephydatia fluviatilis (カワカイメンにおけるRNAi法確立の試み) (2011)
  • Author(s): 岡本和子・阿形清和・船山典子
  • Organizer: 第44回日本発生生物学会
  • Place of Presentation: 沖縄コンベンションセンター(沖縄県)
  • Year and Date: 2011-05-19
• [Presentation] カイメンの秩序立った骨片骨格形成に学ぶ起源的なパターン形成 (2011)
  • Author(s): 中田裕大・中山創平・有馬和志・阿形清和・船山典子
  • Organizer: 日本動物学会近畿支部会
  • Place of Presentation: 京都大学(京都市)
  • Year and Date: 2011-05-14
• [Presentation] Q : What can we learn from sponges? A : The origin of stem cells and pattern forma tion(カイメンから何が学べるのか。幹細胞の起源をパターン形成) (2011)
  • Author(s): 船山典子
  • Organizer: 遺伝学研究所セミナー
  • Place of Presentation: 国立遺伝学研究所(静岡県)(招待講演)
  • Year and Date: 2011-04-25
• [Presentation] Spiculous skeleton formation as a new model to clarify pattern formation in demosponges: The foughly spaced spicule holding up (SHU)points and the identification of spicule carrying cells (2010)
  • Author(s): Sohei Nakayama, Kazushi Arima, Kurato Mohri, Noriko Funayama
  • Organizer: VIII World Sponge Conference
  • Place of Presentation: Spein, Girona
  • Year and Date: 2010-09-22
• [Presentation] Spiculous skeleton formation as a new model to reveal positional infromation and mechanisms of response to it in sponges (2010)
  • Author(s): Kazushi Arima, Sohei Nakayama, Kiyokazu Agata and Noriko Funayama
  • Organizer: 第43回日本発生生物学会
  • Place of Presentation: 京都国際会館(京都市)
  • Year and Date: 2010-06-22
• [Presentation] Studies on the mode of cell division during differentiation of archeocytes (pluripotent stem cells) in sponge,Epfydatia fluviatilis (2010)
  • Author(s): Kazuko Okamoto, Mikiko Nakatsukasa, Kiyokazu Agata, Noriko Funayama
  • Organizer: 第43回日本発生生物学会
  • Place of Presentation: 京都国際会館(京都市)
  • Year and Date: 2010-06-21
• [Presentation] Studies on the mode of cell division during differentiation of archeocytes (pluripotent stem cells) in sponge、Epfydatia fluviatilis (2010)
  • Author(s): Kazuko Okamoto, Mikiko Nakatsukasa, Kiyokazu Agata, Noriko
  • Organizer: VIII World Sponge Conference
  • Place of Presentation: Spein, Girona