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Specification mechanisms of three embryonic axes (animal-vegetal, anteior-posterior, and left-right axes) during ascidian embryogenesis

Research Project

Project/Area Number 22370078
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Developmental biology
Research InstitutionOsaka University

Principal Investigator

NISHIDA Hiroki  大阪大学, 理学(系)研究科(研究院), 教授 (60192689)

Co-Investigator(Kenkyū-buntansha) KUMANO Gaku  大阪大学, 大学院理学研究科, 助教 (80372605)
NISHINO Atsuo  大阪大学, 大学院理学研究科, 助教 (50343116)
Project Period (FY) 2010-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥18,850,000 (Direct Cost: ¥14,500,000、Indirect Cost: ¥4,350,000)
Fiscal Year 2013: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2012: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2011: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2010: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Keywords胚葉形成 / 生殖細胞 / 左右非対称性 / ホヤ / オタマボヤ / 胚軸 / 胚発生 / 前後軸 / 動植軸 / Not遺伝子 / 局在mRNA / 中胚葉 / Noda1 / 尾索動物 / Nodal
Outline of Final Research Achievements

The adult bodies of most animals have three perpendicular axes. These body axes are established during early embryogenesis. It is important to know how the direction and polarity of these axes are specified and how various tissues are patterned along these axes. In this project, we analyzed the mechanisms of patterning of some cell types along three embryonic axes, which are the animal-vegetal, anterior-posterior, and left-right axes, using urochordates (tunicates); the ascidian, Halocynthia roretzi, and the appendicularian, Oikopleura dioica.

Report

(5 results)
  • 2014 Final Research Report ( PDF )
  • 2013 Annual Research Report
  • 2012 Annual Research Report
  • 2011 Annual Research Report
  • 2010 Annual Research Report
  • Research Products

    (54 results)

All 2015 2014 2013 2012 2011 2010 Other

All Journal Article (23 results) (of which Peer Reviewed: 23 results,  Open Access: 4 results,  Acknowledgement Compliant: 7 results) Presentation (26 results) (of which Invited: 7 results) Book (1 results) Remarks (4 results)

  • [Journal Article] Guidelines for the nomenclature of genetic elements in tunicate genomes.2015

    • Author(s)
      Stolfi, A., Sasakura, Y., Chalopin, D., Satou, Y., Christiaen, L., Dantec, C., Endo, T., Naville, M., Nishida, H., Swalla, B., Volff J-N, Voskoboynik, A., Dauga, D., and Lemaire, P.
    • Journal Title

      Genesis

      Volume: 53 Issue: 1 Pages: 1-14

    • DOI

      10.1002/dvg.22822

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] DNA interference: DNA-induced gene silencing in the appendicularian Oikopleura dioica.2015

    • Author(s)
      Omotezako, T., Onuma, T. A., and Nishida, H.
    • Journal Title

      Proc. R. Soc. B

      Volume: in press

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] REGULATOR: a database of metazoan transcription factors and maternal factors for developmental studies.2015

    • Author(s)
      Wang, K., and Nishida, H.
    • Journal Title

      BMC Bioinformatics

      Volume: in press

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Regulation of the number of cell division rounds by tissue-specific transcription factors and Cdk inhibitor during ascidian embryogenesis.2014

    • Author(s)
      Kuwajima, M., Kumano, G., and Nishida, H.
    • Journal Title

      PLOS ONE

      Volume: 9 Issue: 3 Pages: e90188-e90188

    • DOI

      10.1371/journal.pone.0090188

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Transcription factor Tbx6 plays a central role in fate determination between mesenchyme and muscle in embryos of the ascidian, Halocynthia roretzi.2014

    • Author(s)
      Kumano, G., Negoro, N., and Nishida, H.
    • Journal Title

      Dev. Growth Differ.

      Volume: 56 Issue: 4 Pages: 310-322

    • DOI

      10.1111/dgd.12133

    • NAID

      40020059339

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Control of the number of cell division rounds in distinct tissues during ascidian embryogenesis.2014

    • Author(s)
      Yamada, A., and Nishida, H.
    • Journal Title

      Dev. Growth Differ.

      Volume: 56 Issue: 5 Pages: 376-386

    • DOI

      10.1111/dgd.12141

    • NAID

      40020109517

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Cell lineages and fate maps in tunicates: Conservation and modification2014

    • Author(s)
      Nishida, H., and Stach, T.
    • Journal Title

      Zool. Sci.

      Volume: 31 Issue: 10 Pages: 645-652

    • DOI

      10.2108/zs140117

    • NAID

      40020231557

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Long-distance cell migrations during larval development in the appendicularian, Oikopleura dioica.2014

    • Author(s)
      Kanae Kishi, Takeshi A. Onuma, and Hiroki Nishida.
    • Journal Title

      Developmental Biology

      Volume: 395(2) Issue: 2 Pages: 299-306

    • DOI

      10.1016/j.ydbio.2014.09.006

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] RNA interference in the appendicularian Oikopleura dioica reveals the function of the Brachyury gene.2013

    • Author(s)
      Omotezako, T., Nishino, A., Onuma T. A., and Nishida, H.
    • Journal Title

      Dev. Genes Evol.

      Volume: 223 Issue: 4 Pages: 261-267

    • DOI

      10.1007/s00427-013-0438-8

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The maternal muscle determinant in the ascidian egg2012

    • Author(s)
      Nishida, H.
    • Journal Title

      WIREs Dev.Biol.

      Volume: (印刷中) Issue: 3 Pages: 425-433

    • DOI

      10.1002/wdev.22

    • Related Report
      2012 Annual Research Report 2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Cytoplasmic localization and reorganization in ascidian eggs : Role of postplasmic/PEM RNAs in axis formation and fate determination2012

    • Author(s)
      Makabe, K., Nishida, H.
    • Journal Title

      WIRES Dev.Biol.

      Volume: (印刷中) Issue: 4 Pages: 501-518

    • DOI

      10.1002/wdev.54

    • Related Report
      2012 Annual Research Report 2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Neurula rotation determines left-right asymmetry in ascidian tadpole larvae2012

    • Author(s)
      Nishide, K., Nishida, H.
    • Journal Title

      Development

      Volume: 139 Issue: 8 Pages: 1467-1475

    • DOI

      10.1242/dev.076083

    • Related Report
      2012 Annual Research Report 2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The transcription factor FoxB mediates temporal loss of cellular competence for notochord induction in ascidian embryos2011

    • Author(s)
      Hashimoto, H., Nishida, H.
    • Journal Title

      Development

      Volume: 138 Issue: 14 Pages: 2591-2600

    • DOI

      10.1242/dev.070383

    • Related Report
      2011 Annual Research Report 2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Tissue-specific regulation of the number of cell division rounds by inductive cell interaction and transcription factors during ascidian embryogenesis2011

    • Author(s)
      Fujikawa T, Takatori N, Kuwajima M, Kim GJ, Nishida H
    • Journal Title

      Developmental Biology

      Volume: 355 Issue: 2 Pages: 313-23

    • DOI

      10.1016/j.ydbio.2011.04.033

    • Related Report
      2011 Annual Research Report 2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A maternal factor unique to ascidians silences the germline via binding to P-TEFb and RNAP II regulation2011

    • Author(s)
      Kumano G, Takatori N, Negishi T, Takada T, Nishida H
    • Journal Title

      Current Biology

      Volume: 21 Issue: 15 Pages: 1308-13

    • DOI

      10.1016/j.cub.2011.06.050

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Simple procedure for sperm cryopreservation in the larvacean tunicate Oikopleura dioica2011

    • Author(s)
      Ouchi, K., Nishida, H.
    • Journal Title

      Zool.Sci.

      Volume: 28 Pages: 8-11

    • NAID

      210000169624

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Polo-like kinase 1 is required for localization of PEM protein to the centrosome-attracting body and unequal cleavages in ascidian embryos2011

    • Author(s)
      Negishi, T., Nishida, H.
    • Journal Title

      Dev.Growth Differ.

      Volume: 53 Pages: 76-87

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] PEM, a maternal factor unique to ascidians, silences the germline via RNAP II regulation and binding to P-TEFb2011

    • Author(s)
      Kumano, G., Nishida, H.
    • Journal Title

      Curr.Biol.

      Volume: 21 Pages: 1308-1313

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Spatial and temporal expression of two transcriptional isoforms of Lhx3, a LIM class homeobox gene, during embryogenesis of two phylogenetically remote ascidians, Halocynthia roretzi and Ciona intestinalis2010

    • Author(s)
      Kobayashi, M., Nishida, H.
    • Journal Title

      Gene Expression Patterns

      Volume: 10 Pages: 98-104

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Dual mechanism controls asymmetric spindle position in ascidian germ cell precursors2010

    • Author(s)
      Prodon, F., Nishida, H.
    • Journal Title

      Development

      Volume: 344 Pages: 284-292

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Macho-1 regulates unequal cell divisions independently of its function as a muscle determinant2010

    • Author(s)
      Kumano, G., Nishida, H.
    • Journal Title

      Dev.Biol.

      Volume: 344 Pages: 284-292

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Segregation of germ layer fates by nuclear migration-dependent localization of Not mRNA2010

    • Author(s)
      Takatori, N., Nishida, H.
    • Journal Title

      Dev.Cell

      Volume: 19 Pages: 589-598

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Plasticity of animal genome architecture unmasked by rapid evolution of a pelagic tunicate2010

    • Author(s)
      Denoeud, F., Nishida, H.
    • Journal Title

      Science

      Volume: 330 Pages: 1381-1385

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Presentation] RNA interference and DNA interference: Two gene silencing systems in the appendicularian Oikopleura dioica2014

    • Author(s)
      表迫竜也、小沼健、西田宏記
    • Organizer
      日本分子生物学会
    • Place of Presentation
      パシフィコ横浜(横浜)
    • Year and Date
      2014-11-26
    • Related Report
      2013 Annual Research Report
  • [Presentation] オタマボヤを用いた発生額研究の近況2014

    • Author(s)
      小沼健、表迫竜也、岸香苗、西田宏記
    • Organizer
      ホヤ研究会
    • Place of Presentation
      筑波大学東京キャンパス(東京)
    • Year and Date
      2014-10-13
    • Related Report
      2013 Annual Research Report
  • [Presentation] ワカレオタマボヤ幼生期における三種の長距離移動細胞の移動メカニズム2014

    • Author(s)
      岸香苗、小沼健、西田宏記
    • Organizer
      日本動物学会
    • Place of Presentation
      東北大学川内北キャンパス(仙台)
    • Year and Date
      2014-09-11
    • Related Report
      2013 Annual Research Report
  • [Presentation] 脊索動物ワカレオタマボヤの左右非対称性の形成:2細胞期胚の左右割球の標識追跡2014

    • Author(s)
      林桃子、小沼健、西田宏記
    • Organizer
      日本動物学会
    • Place of Presentation
      東北大学川内北キャンパス(仙台)
    • Year and Date
      2014-09-11
    • Related Report
      2013 Annual Research Report
  • [Presentation] 脊索動物ワカレオタマボヤの走査型電子顕微鏡による観察2014

    • Author(s)
      小沼健、磯部美穂、西田宏記
    • Organizer
      日本動物学会
    • Place of Presentation
      東北大学川内北キャンパス(仙台)
    • Year and Date
      2014-09-11
    • Related Report
      2013 Annual Research Report
  • [Presentation] 新しい海産モデル動物としてのオタマボヤの秘めたる可能性2013

    • Author(s)
      西田宏記
    • Organizer
      日本動物学会
    • Place of Presentation
      岡山大学(岡山)
    • Year and Date
      2013-09-26 – 2013-09-28
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] ワカレオタマボヤ幼生期の蛍光ライブイメージング2013

    • Author(s)
      岸香苗、小沼健、西田宏記
    • Organizer
      日本動物学会
    • Place of Presentation
      岡山大学(岡山)
    • Year and Date
      2013-09-26
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] PI3K-mediated migration of the nucleus transcrinbing the Not mRNA in asymmetric ell divisions separates mesoderm and endoderm fates2013

    • Author(s)
      Takatori, N., Saiga, H., and Nishida, H.
    • Organizer
      7th International Tunicate Meeting
    • Place of Presentation
      Napoli (Italy)
    • Year and Date
      2013-07-22 – 2013-07-26
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] Long-distance cell migrations during larval development in tha appendicularian, Oikopleura dioica.2013

    • Author(s)
      Kishi, K., Onuma, T., and Nishida, H.
    • Organizer
      15th International COngress of Developmental Biology
    • Place of Presentation
      Cuncun (Mexico)
    • Year and Date
      2013-06-15 – 2013-06-20
    • Related Report
      2013 Annual Research Report
  • [Presentation] Roles for maternally-localized PEM in ascidian germline development2012

    • Author(s)
      Kumano, G., Negishi, T., Takatori, N., Nishida, H.
    • Organizer
      日本発生生物学会
    • Place of Presentation
      神戸国際会議場( 神戸)
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Presentation] A localized factor polarizes mesendoderm cells and separates mesoderm and endoderm fates in the ascidian ebryos2012

    • Author(s)
      Takatori, N., Nishida, H.
    • Organizer
      日本発生生物学会
    • Place of Presentation
      神戸国際会議場( 神戸)
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Presentation] RNA interference method in the appendicularian, Oikopleura dioica2012

    • Author(s)
      Omotezako, T., Nishino, A., Nishida, H.
    • Organizer
      日本発生生物学会
    • Place of Presentation
      神戸国際会議場( 神戸)
    • Related Report
      2012 Annual Research Report
  • [Presentation] マボヤとアカボヤのゲノム配列決定の現状2012

    • Author(s)
      西田宏記
    • Organizer
      日本動物学会
    • Place of Presentation
      大阪大学( 大阪)
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Presentation] Not mRNA非対称分配による中胚葉と内胚葉の分離機構2012

    • Author(s)
      高鳥直士、熊野岳、西駕秀俊、西田宏記
    • Organizer
      大阪大学( 大阪)
    • Place of Presentation
      大阪大学( 大阪)
    • Related Report
      2012 Annual Research Report
  • [Presentation] ホヤ胚発生における発生運命決定と細胞分裂の回数制御の関係2012

    • Author(s)
      桑島真美、熊野岳、西田宏記
    • Organizer
      大阪大学( 大阪)
    • Place of Presentation
      大阪大学( 大阪)
    • Related Report
      2012 Annual Research Report
  • [Presentation] 内胚葉と中胚葉を分ける非対称細胞分裂における転写中の核の移動を介したNot mRNAの非対称分配2012

    • Author(s)
      高鳥直士、西田宏記
    • Organizer
      日本分子生物学会
    • Place of Presentation
      福岡国際会議場( 福岡)
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Presentation] mRNA非対称分配による胚葉運命分離機構2012

    • Author(s)
      高鳥直士、西駕秀俊、西田宏記
    • Organizer
      日本分子生物学会
    • Place of Presentation
      福岡国際会議場( 福岡)
    • Related Report
      2012 Annual Research Report
  • [Presentation] マボヤ胚の中内胚葉分離におけるNot mRNA非対称分配のメカニズム2011

    • Author(s)
      齊藤由佳、高鳥直士、西田宏記
    • Organizer
      日本動物学会
    • Place of Presentation
      旭川市大雪クリスタルホール(北海道)
    • Year and Date
      2011-09-21
    • Related Report
      2011 Annual Research Report
  • [Presentation] マボヤ胚母性局在因子PEMによる生殖細胞系列での転写抑制機構2011

    • Author(s)
      熊野岳、高鳥直士、根岸剛文、高田樹、西田宏記
    • Organizer
      日本動物学会
    • Place of Presentation
      旭川市大雪クリスタルホール(北海道)
    • Year and Date
      2011-09-21
    • Related Report
      2011 Annual Research Report
  • [Presentation] オタマボヤにおけるRNAiの試み2011

    • Author(s)
      表迫竜也、西野敦雄、西田宏記
    • Organizer
      日本動物学会
    • Place of Presentation
      旭川市大雪クリスタルホール(北海道)
    • Year and Date
      2011-09-21
    • Related Report
      2011 Annual Research Report
  • [Presentation] How do ascidian embryos count the cell division rounds in each tissue lineage?2011

    • Author(s)
      西田宏記
    • Organizer
      6th International Tunicate Meeting
    • Place of Presentation
      McGill University(モントリオール、カナダ)(招待講演)
    • Year and Date
      2011-07-03
    • Related Report
      2011 Annual Research Report
  • [Presentation] How do ascidian embryos count the cell division rounds in each tissue 1 ineage?2011

    • Author(s)
      Hiroki Nishida
    • Organizer
      6th International tunicate meeting
    • Place of Presentation
      McGill Inivertsity(モントリオール、カナダ)(招待講演)
    • Year and Date
      2011-06-05
    • Related Report
      2010 Annual Research Report
  • [Presentation] Expectation from the community II. Cell Bilogy2010

    • Author(s)
      Hiroki Nishida
    • Organizer
      1st Tunicate Information System Meeting
    • Place of Presentation
      Hotel Le Royal(ニース、フランス)(招待講演)
    • Year and Date
      2010-11-11
    • Related Report
      2010 Annual Research Report
  • [Presentation] オタマボヤにおけるCAB様構造体の解析2010

    • Author(s)
      幸西翔平、西田宏記
    • Organizer
      日本動物学会
    • Place of Presentation
      東京大学駒場キャンパス
    • Year and Date
      2010-09-23
    • Related Report
      2010 Annual Research Report
  • [Presentation] Neurula rotation determines left-right asymmetry in ascidian tadpole Larva2010

    • Author(s)
      Nishide, K., Nishida, H
    • Organizer
      日本発生生物学会
    • Place of Presentation
      京都国際会議場
    • Year and Date
      2010-06-20
    • Related Report
      2010 Annual Research Report
  • [Presentation] How do embryos count the cell division rounds in each tissue lineage? : An ascidian case2010

    • Author(s)
      Hiroki Nishida
    • Organizer
      2nd Joint Meeting of the SFBD and JSDB
    • Place of Presentation
      パスツール研究所(パリ、フランス)(招待講演)
    • Year and Date
      2010-05-26
    • Related Report
      2010 Annual Research Report
  • [Book] 細胞周期フロンティア2010

    • Author(s)
      西田宏記, 他73名、共著
    • Publisher
      共立出版社
    • Related Report
      2010 Annual Research Report
  • [Remarks] 西田研究室ホームページ

    • URL

      http://www.bio.sci.osaka-u.ac.jp/bio_web/lab_page/nishida/index.html

    • Related Report
      2013 Annual Research Report
  • [Remarks] 西田研究室

    • URL

      http://www.bio.sci.osaka-u.ac.jp/bio_web/lab_page/nishida/index.html

    • Related Report
      2012 Annual Research Report
  • [Remarks]

    • URL

      http://www.bio.sci.osaka-u.ac.jp/bio_web/lab_page/nishida/naiyou.html

    • Related Report
      2011 Annual Research Report
  • [Remarks]

    • URL

      http://www.bio.sci.osaka-u.ac.jp/bio_web/lab_page/nishida/naiyou.html

    • Related Report
      2010 Annual Research Report

URL: 

Published: 2010-08-23   Modified: 2019-07-29  

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