A study on polymorphisms associated with obesity and lipid profile in the Oceanic populations

• Project/Area Number: 22370084
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Physical anthropology
• Research Institution: University of Tsukuba
• Principal Investigator: OHASHI Jun 筑波大学, 医学医療系, 准教授 (80301141)
• Co-Investigator(Kenkyū-buntansha): KIMURA Ryosuke 琉球大学, 医学部, 准教授 (00453712) ; NISHIDA Nao 独立行政法人国立国際医療センター, 肝炎・免疫研究センター, 上級研究員 (50456109) ; NAKAYAMA Kazuhiro 自治医科大学, 医学部, 講師 (90433581)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥19,890,000 (Direct Cost: ¥15,300,000、Indirect Cost: ¥4,590,000); Fiscal Year 2012: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2011: ¥6,630,000 (Direct Cost: ¥5,100,000、Indirect Cost: ¥1,530,000); Fiscal Year 2010: ¥8,060,000 (Direct Cost: ¥6,200,000、Indirect Cost: ¥1,860,000)
• Keywords: オセアニア / 肥満 / 多型 / 倹約遺伝子 / BMI / 脂質代謝 / オーストロネシア語族 / 遺伝子 / 倹約遺伝子仮説 / 糖代謝

Research Abstract

A SNP located upstream of ADRB2, rs34623097, showed the strongest association with obesity in a logistic regression analysis adjusted for age, sex, and population (P = 5.6 x 10-4, odds ratio [OR] = 2.5, 95% confidence interval [CI] = 1.5-4.2).To study the origin of Polynesians and the gene flow from Polynesian ancestors to indigenous Melanesians, a 48-bp variable number of tandem repeat (VNTR) in exon 3 of the dopamine D4 receptor (DRD4) gene was investigated for six Austronesian (AN)-speaking populations. Our results provide evidence of gene flow from Polynesian ancestors to indigenous Melanesians while Polynesian ancestors passed through Melanesia.A candidate gene association analysis on 11 metabolic traits of 5,810 East Asians revealed that rs1057001, a T/A transversion polymorphism in 3'untranslated region (UTR) of TRIB2, was strongly associated with visceral fat area (VFA) and waist circumference adjusted for body mass index (P = 2.7 × 10(-6) and P = 9.0 × 10(-6), respectively).

Research Products

• [Journal Article] A "functional SNP upstream of the beta-2 adrenergic receptor gene (ADRE2)is associated with obesity in Oceanic populations (2013)
  • Author(s): Naka l
  • Journal Title: International Journal of Obesity Volume: (in press) Issue: 9 Pages: 1204-1210
  • DOI: 10.1038/ijo.2012.206
  • Peer Reviewed
• [Journal Article] Positive natural selection of TRIB2, a novel gene that influences visceral fat accumulation, in East Asia (2013)
  • Author(s): Nakayama K, Ogawa A, Miyashita H, Tabara Y, Igase M, Kohara K, Miki T, Kagawa Y, Yanagisawa Y, Katashima M, Onda T, Okada K, Fukushima S, Iwamoto S
  • Journal Title: Hum Genet Volume: 132 Pages: 201-217
  • Peer Reviewed
• [Journal Article] Hypertension Susceptibility Gene Prevalence in the Pacific Islands and Associations with Hypertension in Melanesia. (2013)
  • Author(s): Furusawa T, Naka I, Yamauchi T, Natsuhara K, Eddie R, Kimura R, Nakazawa M, Ishida T, Inaoka T, Matsumura Y, Ataka Y, Ohtsuka R, Ohashi J
  • Journal Title: Journal of Human Genetics Volume: 58 Pages: 142-149
  • NAID: 10031164071
  • Peer Reviewed
• [Journal Article] Plasma resistin is associated with single nucleotide polymorphisms of a possible resistin receptor, the decorin gene, in the general Japanese population. (2013)
  • Author(s): Onuma H, Tabara Y, Kawamura R, Ohashi J, Nishida W, Takata Y, Ochi M, Nishimiya T, Kawamoto R, Kohara K, Miki T, Osawa H
  • Journal Title: Diabetes Volume: 62 Pages: 649-652
  • Peer Reviewed
• [Journal Article] Positive natural selection of TRIB2, a novel gene that influences visceral fat accumulation, in East Asia. (2013)
  • Author(s): Nakayama K, Ogawa A, Miyashita H, Tabara Y, Igase M, Kohara K, Miki T, Kagawa Y, Yanagisawa Y, Katashima M, Onda T, Okada K, Fukushima S, Iwamoto S
  • Journal Title: Hum Genet Volume: 132 Issue: 2 Pages: 201-217
  • DOI: 10.1007/s00439-012-1240-9
  • Peer Reviewed
• [Journal Article] Hypertension Susceptibility Gene Prevalence in the Pacific Islands and Associations with Hypertension in Melanesia (2013)
  • Author(s): Furusawa T
  • Journal Title: Journal of Human Genetics Volume: (in press) Issue: 3 Pages: 142-149
  • DOI: 10.1038/jhg.2012.147
  • Peer Reviewed
• [Journal Article] DRD4 VNTR polymorphism in Oceanic populations. (2012)
  • Author(s): Naka I, Ohashi J, Kimura R, Furusawa T, Yamauchi T, Nakazawa M, Natsuhara K, Ataka Y, Nishida N, Ishida T, Inaoka T, Matsumura Y, Ohtsuka R
  • Journal Title: Anthropological Science Volume: 120 Pages: 151-155
  • NAID: 10031057349
  • Peer Reviewed
• [Journal Article] Association of CTXN3-SLC12A2 polymorphisms and schizophrenia in a Thai population (2012)
  • Author(s): Panichareon B, Nakayama K, Iwamoto S, Thurakitwannakarn W, Sukhumsirichart W
  • Journal Title: Behav Brain Funct Volume: 8 Pages: 27-27
  • Peer Reviewed
• [Journal Article] OPCML gene as a schizophrenia susceptibility locus in Thai population (2012)
  • Author(s): Panichareon B, Nakayama K, Thurakitwannakarn W, Iwamoto S, Sukhumsirichart W
  • Journal Title: J Mol Neurosci Volume: 46 Pages: 373-377
  • Peer Reviewed
• [Journal Article] The history of human populations in the Japanese Archipelago inferred from genome-wide SNP data with a special reference to the Ainu and the Ryukyuan populations (2012)
  • Author(s): Japanese Archipelago Human Population Genetics Consortium members: Jinam T, Nishida N, Hirai M, Kawamura S, Oota H, Umetsu K, Kimura R, Ohashi J, Tajima A, Yamamoto T, Tanabe H, Mano S, Suto Y, Kaname T, Naritomi K, Yanagi K, Niikawa N, Omoto K, Tokunaga
  • Journal Title: Journal of Human Genetics Volume: 57 Pages: 787-795
  • NAID: 10031145887
  • Peer Reviewed
• [Journal Article] <i>DRD4</i> VNTR polymorphism in Oceanic populations (2012)
  • Author(s): Naka, I. et al.
  • Journal Title: Anthropological Science Volume: 120 Issue: 2 Pages: 151-155
  • DOI: 10.1537/ase.110901
  • NAID: 10031057349
  • ISSN: 0918-7960, 1348-8570
  • Peer Reviewed
• [Journal Article] Highly parallel and short-acting amplification with locus-specific primers to detect single nucleotide polymorphisms by the DigiTag2 assay (2012)
  • Author(s): Nishida et al.
  • Journal Title: PLoS One Volume: 7 Issue: 1 Pages: e29967-e29967
  • DOI: 10.1371/journal.pone.0029967
  • Peer Reviewed
• [Journal Article] DRD4 VNTR polymorphism in Oceanic populations (2012)
  • Author(s): Naka, et al.
  • Journal Title: Anthropological Science Volume: (In press)
  • Peer Reviewed
• [Journal Article] Extended Haplotype Homozygosity (EHH)を用いる正の自然選択検出法の検出力比較 (2012)
  • Author(s): 大橋順
  • Journal Title: 統計数理 Volume: (In press)
  • NAID: 120006020421
  • Peer Reviewed
• [Journal Article] High prevalence of an anti-hypertriglyceridemic variant of the MLXIPL gene in Central Asia (2011)
  • Author(s): Nakayama K, Yanagisawa Y, Ogawa A, Ishizuka Y, Munkhtulga L, Charupoonphol P, Supannnatas S, Kuartei S, Chimedregzen U, Koda Y, Ishida T, Kagawa Y, Iwamoto S
  • Journal Title: J Hum Genet Volume: 56 Pages: 828-833
  • NAID: 10030661852
  • Peer Reviewed
• [Journal Article] No evidence for strong recent positive selection favoring the 7 repeat allele of VNTR in the DRD4 gene (2011)
  • Author(s): Naka I, Nishida N, Ohashi J
  • Journal Title: PLoS One Volume: 6
  • NAID: 120007137473
  • Peer Reviewed
• [Journal Article] Testing the thrifty gene hypothesis: the Gly482Ser variant in PPARGC1A is associated with BMI in Tongans. (2011)
  • Author(s): Myles S, Lea RA, Ohashi J, Chambers GK, Weiss JG, Hardouin E, Engelken J, Macartney-Coxson DP, Eccles DA, Naka I, Kimura R, Inaoka T, Matsumura Y, Stoneking M
  • Journal Title: BMC Medical Genetics Volume: 12 Pages: 10-10
  • Peer Reviewed
• [Journal Article] The serum leptin level and body mass index in Melanesian and Micronesian Solomon Islanders: Focus on genetic factors and urbanization. (2011)
  • Author(s): Furusawa T, Naka I, Yamauchi T, Natsuhara K, Kimura R, Nakazawa M, Ishida T, Nishida N, Eddie R, Ohtsuka R,Ohashi J
  • Journal Title: American Journal of Human Biology Volume: 23 Pages: 435-444
  • Peer Reviewed
• [Journal Article] The impact of natural selection on an ABCC11 SNP determining earwax type. (2011)
  • Author(s): Ohashi J, Naka I, Tsuchiya N
  • Journal Title: Molecular Biology and Evolution Volume: 28 Pages: 849-857
  • Peer Reviewed
• [Journal Article] No evidence for strong recent positive selection favoring the 7 repeat allele of VNTR in the DRD4 gene (2011)
  • Author(s): Naka, et al.
  • Journal Title: PLoS ONE Volume: 6 Issue: 8 Pages: e24410-e24410
  • DOI: 10.1371/journal.pone.0024410
  • Peer Reviewed
• [Journal Article] Testing the thrifty gene hypothesis : the Gly482Ser variant in PPARGC1A is associated with BMI in Tongans (2011)
  • Author(s): Myles, et al.
  • Journal Title: BMC Medical Genetics Volume: 12 Issue: 1
  • DOI: 10.1186/1471-2350-12-10
  • Peer Reviewed
• [Journal Article] The Serum Leptin Level and Body Mass Index in Melanesian and Micronesian Solomon Islanders : Focus on Genetic Factors and Urbanization (2011)
  • Author(s): Furusawa, et al.
  • Journal Title: American Journal of Human Biology Volume: 23 Issue: 4 Pages: 435-444
  • DOI: 10.1002/ajhb.21124
  • Peer Reviewed
• [Journal Article] Testing the thrifty gene hypothesis : the Gly482Ser variant in PPARGC1A is associated with BMI in Tongans (2011)
  • Author(s): Myles S, et al.
  • Journal Title: BMC Medical Genetics Volume: 12 Pages: 10-10
  • Peer Reviewed
• [Journal Article] The Serum Leptin Level and Body Mass Index in Melanesian and Micronesian Solomon Islanders : Focus on Genetic Factors and Urbanization (2011)
  • Author(s): Furusawa T, et al.
  • Journal Title: American Journal of Human Biology Volume: 印刷中
  • Peer Reviewed
• [Journal Article] The impact of natural selection on an ABCC11 SNP determining earwax type (2011)
  • Author(s): Ohashi J, et al.
  • Journal Title: Molecular Biology and Evolution Volume: 28 Pages: 849-857
  • Peer Reviewed
• [Journal Article] Comparative analysis of copy number variation detection methods and database construction (2011)
  • Author(s): Koike A, et al.
  • Journal Title: BMC Genetics Volume: 12 Pages: 29-29
  • Peer Reviewed
• [Journal Article] A single nucleotide polymorphism in the FADS1/FADS2 gene is associated with plasma lipid profiles in two genetically similar Asian ethnic groups with distinctive differences in lifestyle. (2010)
  • Author(s): Nakayama K, Bayasgalan T, Tazoe F, Yanagisawa Y, Gotoh T, Yamanaka K, Ogawa A, Munkhtulga L, Chimedregze U, Kagawa Y, Ishibashi S, Iwamoto S
  • Journal Title: Hum Genet Volume: 127 Pages: 685-690
  • Peer Reviewed
• [Journal Article] The Q223R polymorphism in LEPR is associated with obesity in Pacific Islanders. (2010)
  • Author(s): Furusawa T, Naka I, Yamauchi T, Natsuhara K, Kimura R, Nakazawa M, Ishida T, Inaoka T, Matsumura Y, Ataka Y, Nishida N, Tsuchiya N, Ohtsuka R, Ohashi J
  • Journal Title: Human Genetics Volume: 127 Pages: 287-294
  • Peer Reviewed
• [Journal Article] Regulatory SNP in the RBP4 gene modified the expression in adipocytes and associated with BMI (2010)
  • Author(s): Munkhtulga L, Nagashima S, Nakayama K, Utsumi N, Yanagisawa Y, Gotoh T, Omi T, Kumada M, Zolzaya K, Lkhagvasuren T, Kagawa Y, Fujiwara H, Hosoya Y, Hyodo M, Horie H, Kojima M, Ishibashi S, Iwamoto S
  • Journal Title: Obesity (Silver Spring) Volume: 18 Pages: 1006-1014
  • Peer Reviewed
• [Journal Article] Variations in the coding region of the agouti signaling protein gene do not explain agouti/non-agouti phenotypes in macaques (2010)
  • Author(s): Nakayama K, Shotake T, Takenaka O, Ishida T
  • Journal Title: J Mammal Evol Volume: 17 Pages: 211-214
  • Peer Reviewed
• [Journal Article] The GCKR rs780094 polymorphism is associated with susceptibility of type 2 diabetes, reduced fasting plasma glucose levels, increased triglycerides levels, and lower HOMA-IR in Japanese population. (2010)
  • Author(s): Onuma H, Tabara Y, Kawamoto R, Shimizu I, Kawamura R, Takata Y, Nishida W, Ohashi J, Miki T, Kohara K, Makino H, Osawa H
  • Journal Title: Journal of Human Genetics Volume: 55 Pages: 600-604
  • Peer Reviewed
• [Journal Article] A at Single Nucleotide Polymorphism-358 Is Required for G at -420 to Confer the Highest Plasma Resistin in the General Japanese Population. (2010)
  • Author(s): Onuma H, Tabara Y, Kawamura R, Tanaka T, Ohashi J, Nishida W, Takata Y, Ochi M, Yamada K, Kawamoto R, Kohara K, Miki T, Makino H, Osawa H
  • Journal Title: PLoS One Volume: 5(3)
  • Peer Reviewed
• [Journal Article] Is rs34861192 or rs1862513 a more promising variant for determining plasma resistin in an aged Japanese population? (2010)
  • Author(s): Osawa H, Tabara Y, Ohashi J, Kawamura , Onuma H, Makino H
  • Journal Title: Diabetologia Volume: 53 Pages: 795-797
  • Peer Reviewed
• [Journal Article] The GCKR rs780094 polymorphism is associated with susceptibility of type 2 diabetes, reduced fasting plasma glucose levels, increased triglyce rides levels, and lower HOMA-IR in Japanese population (2010)
  • Author(s): Onuma H, et al.
  • Journal Title: Journal of Human Genetics Volume: 55 Pages: 600-604
  • Peer Reviewed
• [Journal Article] A at Single Nucleotide Polymorphism-358 Is Required for G at-420 to Confer the Highest Plasma Resistin in the General Japanese Population (2010)
  • Author(s): Onuma H, et al.
  • Journal Title: PLoS ONE Volume: 5
  • Peer Reviewed
• [Journal Article] The Q223R polymorphism in LEPR is associated with obesity in Pacific Islanders (2010)
  • Author(s): Furusawa T, et al.
  • Journal Title: Human Genetics Volume: 127 Pages: 287-294
  • Peer Reviewed
• [Journal Article] Transcriptional regulation of endochondral ossification by HIF-2α during skeletal growth and osteoarthritis development (2010)
  • Author(s): Saito T, et al.
  • Journal Title: Nature Medicine Volume: 16 Pages: 678-686
  • Peer Reviewed
• [Presentation] Significant association of LPL polymorphisms with HDL-cholesterol level in Tongan adults (2012)
  • Author(s): Naka et al.
  • Organizer: The American Society of Human Genetics 62th Annual Meeting
  • Place of Presentation: San Francisco, CA, USA
  • Year and Date: 2012-11-09
• [Presentation] β2アドレナリン受容体遺伝子(ADRB2) 上流領域SNPの進化遺伝学的解析 (2012)
  • Author(s): 中伊津美,他
  • Organizer: 日本人類学会第66回大会
  • Place of Presentation: 慶応大学日吉キャンパス、日吉、神奈川
  • Year and Date: 2012-11-04
• [Presentation] トンガ人集団におけるLPL遺伝子多型とHDLコレステロールとの関連 (2012)
  • Author(s): 中伊津美,他
  • Organizer: 日本人類遺伝学会第57回大会
  • Place of Presentation: 新宿、東京
  • Year and Date: 2012-10-25
• [Presentation] オセアニア集団におけるDRD4 遺伝子 VNTR 多型の解析 (2011)
  • Author(s): 中伊津美,他
  • Organizer: 日本人類遺伝学会第56回大会
  • Place of Presentation: 幕張、千葉
  • Year and Date: 2011-11-11
• [Presentation] オセアニア集団におけるDRD4遺伝子VNTR多型の解析 (2011)
  • Author(s): 中伊津美, ほか
  • Organizer: 日本人類遺伝学会
  • Place of Presentation: 幕張、千葉
  • Year and Date: 2011-11-11
• [Presentation] オセアニア集団におけるβアドレナリン受容体プロモーター多型と肥満との関連 (2011)
  • Author(s): 中伊津美,他
  • Organizer: 日本人類学会第65回大会
  • Place of Presentation: 那覇、沖縄
  • Year and Date: 2011-11-04
• [Presentation] The VNTR polymorphism of the dopamine D4 receptor (DRD4) gene in Oceanic populations (2011)
  • Author(s): Naka et al.
  • Organizer: The American Society of Human Genetics 60th Annual Meeting, The American Society of Human Genetics 60th Annual Meeting
  • Place of Presentation: Washington, USA
  • Year and Date: 2011-11-04
• [Presentation] オセアニア集団におけるβアドレナリン受容体プロモーター多型と肥満との関連 (2011)
  • Author(s): 中伊津美, ほか
  • Organizer: 日本人類学会
  • Place of Presentation: 那覇、沖縄
  • Year and Date: 2011-11-04
• [Presentation] Significant association of ADRB2 Glu27Gln polymorphism with triglyceride level (2011)
  • Author(s): Naka et al.
  • Organizer: The American Society of Human Genetics 61th Annual Meeting
  • Place of Presentation: Montreal, Canada
  • Year and Date: 2011-10-14
• [Presentation] Significant association of ADRB2 Glu27Gln polymorphism with triglyceride level (2011)
  • Author(s): Naka I, et al.
  • Organizer: American Society of Human Genetics
  • Place of Presentation: Montreal, Canada
  • Year and Date: 2011-10-14
• [Presentation] The VNTR polymorphism of the dopamine D4 receptor (DRD4) genein Oceanic populations (2010)
  • Author(s): Naka I, et al.
  • Organizer: The American Society of Human Genetics 60^<th> Annual Meeting
  • Place of Presentation: ワシントンDC・アメリカ合衆国
  • Year and Date: 2010-11-03
• [Presentation] β2アドレナリン受容体遺伝子(ADRB2) 上流領域SNPの進化遺伝学的解析
  • Author(s): 中 伊津美 ほか
  • Organizer: 日本人類学会第66回大会
  • Place of Presentation: 神奈川
• [Presentation] トンガ人集団におけるLPL遺伝子多型とHDLコレステロールとの関連
  • Author(s): 中 伊津美 ほか
  • Organizer: 日本人類遺伝学会第57回大会
  • Place of Presentation: 東京
• [Presentation] Significant association of LPL polymorphisms with HDL-cholesterol level in Tongan adults
  • Author(s): Naka et al.
  • Organizer: The American Society of Human Genetics 62th Annual Meeting
  • Place of Presentation: San Francisco (USA)
• [Remarks]
  • URL: http://www.md.tsukuba.ac.jp/community-med/publicmd/GE/CCP004.html
• [Remarks]
  • URL: http://www.md.tsukuba.ac.jp/community-med/publicmd/GE/CCP004.html