| Budget Amount *help |
¥16,900,000 (Direct Cost: ¥13,000,000、Indirect Cost: ¥3,900,000)
Fiscal Year 2013: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Fiscal Year 2012: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2011: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2010: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
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| Research Abstract |
Using the scion in which the construct producing an artificial siRNA sequence was integrated, its siRNA molecule transported through phloem functions to induce the silencing (TGS: transcriptional gene silencing) of the gene possessing complementary sequence with the siRNA. In case of TGS, as the invoked silencing maintains through DNA replication, the TGS plant can be obtained by the regeneration from the TGS tissue. This study was carried out the each step experiment in order to apply the above system to a vegetative crop, apple (Malus domestica).
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