Molecular cytogenetic analyses on crossing barrier between common wheat and its alien species of Triticeae

• Project/Area Number: 22380004
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Breeding science
• Research Institution: Chiba University
• Principal Investigator: KOBA Takato 千葉大学, 大学院・園芸学研究科, 教授 (40170302)
• Co-Investigator(Kenkyū-buntansha): SASSA Hidenori 千葉大学, 大学院・園芸学研究科, 准教授 (50295507) ; KIKUCHI Shinji 千葉大学, 大学院・園芸学研究科, 助教 (80457168)
• Research Collaborator: MISHINA Kouhei 横浜市立大学, 木原生物学研究所, 研究員
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥18,980,000 (Direct Cost: ¥14,600,000、Indirect Cost: ¥4,380,000); Fiscal Year 2012: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2011: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2010: ¥10,140,000 (Direct Cost: ¥7,800,000、Indirect Cost: ¥2,340,000)
• Keywords: パンコムギ / 交雑親和性 / QTL 解析 / 染色体置換系統 / 組換え近交系統 / 分子マーカー / 花粉管伸長 / 共焦点レーザー顕微鏡 / QTL解析 / 育種学 / 花粉管 / RIL / 5B染色体 / 遺伝子 / ゲノム / 植物 / マッピング

Research Abstract

QTL analysis on the crossability of common wheat with rye using recombinant inbred lines (RILs) developed from inter-varietal chromosome substitution lines clarified that the gene for crossability was located on the short arm of common wheat. Observation of pollen tube elongation of rye pollen on the common wheat pistils showed high correlation between pollen tube elongation and the degrees of crossabilty. These facts show that Skr gene located on the short arm of chromosome 5B inhibits pollen elongation in the styles of common wheat.

Research Products

• [Presentation] Chromosomal stability in amphidiploids between tetraploid wheat cv. Langdon and Aegilops uniaristata and their progenies (2013)
  • Author(s): Koba, T., K. Nagataki, S. Takumi,S. Kikuchi, K. Mishina and H. Sassa
  • Organizer: 12^<th> International Wheat GeneticsSymposium, Yokohama, Japan
• [Presentation] Re-localization of the gene for crossability on chromosome 5B of common wheat (2011)
  • Author(s): 三科興平
  • Organizer: 26^<th> Internatinal Triticeae Mapping Initiative Symposium
  • Place of Presentation: メキシコシティー
  • Year and Date: 2011-09-05
• [Presentation] Identification of wheat-barley addition lines using EST markers mapped in barley (2011)
  • Author(s): 木庭卓人
  • Organizer: 26^<th> Internatinal Ttiticeae Mapping Initiative Symposium
  • Place of Presentation: メキシコシティー
  • Year and Date: 2011-09-05
• [Presentation] Re-localization of the gene for crossability on chromosome 5B ofcommon wheat (2011)
  • Author(s): Mishina, K., H. Sassa and T. Koba
  • Organizer: 26^<th> InternationalTriticeae Mapping InitiativeSymposium, Mexico City, Mexico
• [Presentation] Identification of wheat-barley addition lines using EST markers mapped in barley (2011)
  • Author(s): Koba, T.,T. Kawaguchi, K. Mishina, S. Kikuchi and H. Sassa
  • Organizer: 26^<th> International Triticeae Mapping Initiative Symposium, Mexico City, Mexico