| Budget Amount *help |
¥18,850,000 (Direct Cost: ¥14,500,000、Indirect Cost: ¥4,350,000)
Fiscal Year 2013: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2012: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2011: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2010: ¥8,320,000 (Direct Cost: ¥6,400,000、Indirect Cost: ¥1,920,000)
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| Research Abstract |
The rice transposon mPing is expected as a genetic tool to create genes showing
novel expression profiles since the mPing insertions render adjacent genes stress inducible. In this study, we first established the screening system for mutant genes harboring de novo mPing insertion into the upstream region. Then, we evaluated the effect of the mPing insertion on the expression profiles of stress-response genes. Furthermore, we investigated the methylation degree of promoter region of stress-response genes harboring the mPing insertion. It was found that mPing could confer various expression profiles to stress-response genes independent of DNA methylation.
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