Studies on molecular mechanisms for radish male sterility/fertilityrestoration system and its diversification
| Project/Area Number |
22380008
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Breeding science
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| Research Institution | Kyoto Sangyo University |
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Principal Investigator |
TERACHI Toru 京都産業大学, 総合生命科学部, 教授 (90202192)
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| Co-Investigator(Kenkyū-buntansha) |
YAMAGISHI Hiroshi 京都産業大学, 総合生命科学部, 教授 (10210345)
KUROSAKA Akira 京都産業大学, 総合生命科学部, 教授 (90186536)
TAKAHASHI Ryo 京都産業大学, 総合生命科学部, 助教 (50342811)
NAKAMURA Takahiro 九州大学, 農学研究科, 准教授 (10464398)
MIYAKE Keiko 岐阜大学, 男女共同参画推進室, 准教授 (40404058)
KOIZUKA Nobuya 玉川大学, 農学部, 教授 (30433866)
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| Project Period (FY) |
2010 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥18,850,000 (Direct Cost: ¥14,500,000、Indirect Cost: ¥4,350,000)
Fiscal Year 2012: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2011: ¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000)
Fiscal Year 2010: ¥7,280,000 (Direct Cost: ¥5,600,000、Indirect Cost: ¥1,680,000)
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| Keywords | ダイコン / 雄性不稔 / 稔性回復遺伝子 / PPR / ミトコンドリア / ハマダイコン / オグラ型 / コセナ型 / orf125 / 次世代シークエンサー |
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| Research Abstract |
In order to reveal molecular mechanisms underlying malesterility/fertility restoration in radish and the evolution of mitochondrial and nuclear genesrelevant to the phenomena, a series of experiments were conducted. Molecular studyrevealed that protein product from the gene pprB (one of the fertility restorer genes inradish) binds to mRNA of the mitochondrial male-sterility gene orf138 and inhibit itstranslation through the structural change of mRNA. Mitochondrial genomics revealedthat orf138 was created accidentally by the structural changes of mitochondrial genomesbetween normal and Ogura cytoplasm.
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Report
(4 results)
Research Products
(29 results)
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[Journal Article] Identification and characterization of the RNA binding surface of the pentatricopeptide repeat protein2012
Author(s)
Kobayashi, K., Kawabata, M., Hisano, K.,Kazama, T., Matsuoka, K., Sugita, M. and Nakamura, T
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Journal Title
Nucleic Acids Res
Volume: 40
Pages: 2712-2723
Related Report
Peer Reviewed
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