Digital gene expression QTL analysisas an efficient gene mapping tool
| Project/Area Number |
22380009
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Breeding science
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| Research Institution | Shinshu University |
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Principal Investigator |
MATSUMURA Hideo 信州大学, ヒト環境科学研究支援センター, 准教授 (40390885)
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| Project Period (FY) |
2010 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥18,460,000 (Direct Cost: ¥14,200,000、Indirect Cost: ¥4,260,000)
Fiscal Year 2012: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000)
Fiscal Year 2011: ¥6,110,000 (Direct Cost: ¥4,700,000、Indirect Cost: ¥1,410,000)
Fiscal Year 2010: ¥6,630,000 (Direct Cost: ¥5,100,000、Indirect Cost: ¥1,530,000)
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| Keywords | ゲノム育種 / 遺伝子発現解析 / DNA マーカー / ゲノム多型 / 遺伝子発現 / 次世代シークエンサー / 連関解析 / イネ / デジタル遺伝子発現解析 / 次世代DNAシークエンサー / 遺伝子発現QTL / Recombinant Inbred Line / 遺伝子型タイピング |
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| Research Abstract |
We aimed at developing a novel QTL (quantitative trait locus) mapping procedure based on genome-wide gene expression analysisdata in rice. In the present study, quantitative digital gene expression analysis method was developed, allowed to analyzing expression of thousands of gene in multiple samples, like segregated individuals. Additionally, a novel tool for exploring DNA polymorphisms was also established and it enabled to genotyping many loci at a time. These techniques were basis of digital expression QTL analysis.
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Report
(4 results)
Research Products
(18 results)
