Study on the mechanism of dual resistance to virus and oomycete using their chimeric resistance genes
Project/Area Number |
22380028
|
Research Category |
Grant-in-Aid for Scientific Research (B)
|
Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Plant pathology
|
Research Institution | Tohoku University |
Principal Investigator |
TAKAHASHI Hideki 東北大学, (連合)農学研究科(研究院), 教授 (20197164)
|
Co-Investigator(Kenkyū-buntansha) |
ANDO Sugihiro 東北大学, 大学院農学研究科, 准教授 (10442831)
|
Project Period (FY) |
2010-04-01 – 2015-03-31
|
Project Status |
Completed (Fiscal Year 2014)
|
Budget Amount *help |
¥18,070,000 (Direct Cost: ¥13,900,000、Indirect Cost: ¥4,170,000)
Fiscal Year 2013: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
Fiscal Year 2012: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2011: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2010: ¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000)
|
Keywords | ウイルス抵抗性 / キュウリモザイクウイルス / 病害抵抗性遺伝子 / NB-LRRタンパク質 |
Outline of Final Research Achievements |
RCY1 confers the hypersensitive resistance (HR) response to Cucumber mosaic virus [CMV(Y)] in A. thaliana, whereas its allelic RPP8 does the resistance to H. arabidopsidis. When RCY1, RPP8 and a series of chimeric constructs between RCY1 and RPP8 were transiently expressed in N. benthamiana, respectively, HR cell death was induced in the leaf tissue expressing chimeric construct carrying CC-NB of RPP8 and LRR of RCY1, suggesting that LRR domain of RCY1 determines the recognition of CMV(Y). Furthermore, to study on the regulatory mechanism of RCY1/RPP8 gene expression, we analyzed the contribution of each structural element of the gene. As the result, we can find that the presence of intron in RCY1/RPP8 genes is indispensable for higher level of RCY1/RPP8 protein accumulation. Those findings regarding pathogen recognition specificity and the role of intron for the resistance to pathogens will give new insight to understand the plant immune system against pathogen infection.
|
Report
(5 results)
Research Products
(26 results)
-
-
[Journal Article] Transcriptional profile of tomato roots exhibiting Bacillus thuringiensis- induced resistance to Ralstonia solanacearum.2014
Author(s)
Takahashi, H., Nakaho, K., Ishihara, T., Ando, S., Wada, T., Kanayama, Y., Asano, S., Yoshida, S., Tsushima, S. and Hyakumachi, M.
-
Journal Title
Plant Cell Reports
Volume: 33
Pages: 99-110
Related Report
Peer Reviewed / Acknowledgement Compliant
-
[Journal Article] The temporal evolution and global spread of Cauliflower mosaic virus, a plant pararetrovirus.2014
Author(s)
Yasaka, R., Nguyen, H.D., Ho, S.Y.W., Duchene, S., Korkmaz, S., Katis, N., Takahashi, H., Gibbs, A.J. and Ohshima, K.
-
Journal Title
PLoS ONE
Volume: 9
Issue: 4
Pages: e95410-e95410
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
-
[Journal Article] Longer uncommon polyamines have a stronger defense gene-induction activity and a higher suppressing activity of Cucumber mosaic virus multiplication compared to that of spermine in Arabidopsis thaliana.2013
Author(s)
Sagor, G.H.M., Liu, T., Takahashi, H., Niitsu, M., Berberich, T. and Kusano, T.
-
Journal Title
Plant Cell Reports
Volume: 32
Pages: 1477-1488
Related Report
Peer Reviewed
-
[Journal Article] Ethylene-responsive AP2/ERF transcription factor MACD1 participates in phytotoxin-triggered programmed cell death2013
Author(s)
Mase, K., Ishihama, N., Mori, H., Takahashi, H., Kaminaka, H., Kodama, M. and Yoshioka, H.
-
Journal Title
Molecular Plant-Microbe Interactions
Volume: 26
Pages: 868-879
Related Report
Peer Reviewed
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-