| Budget Amount *help |
¥18,070,000 (Direct Cost: ¥13,900,000、Indirect Cost: ¥4,170,000)
Fiscal Year 2013: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
Fiscal Year 2012: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2011: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2010: ¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000)
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| Outline of Final Research Achievements |
RCY1 confers the hypersensitive resistance (HR) response to Cucumber mosaic virus [CMV(Y)] in A. thaliana, whereas its allelic RPP8 does the resistance to H. arabidopsidis. When RCY1, RPP8 and a series of chimeric constructs between RCY1 and RPP8 were transiently expressed in N. benthamiana, respectively, HR cell death was induced in the leaf tissue expressing chimeric construct carrying CC-NB of RPP8 and LRR of RCY1, suggesting that LRR domain of RCY1 determines the recognition of CMV(Y). Furthermore, to study on the regulatory mechanism of RCY1/RPP8 gene expression, we analyzed the contribution of each structural element of the gene. As the result, we can find that the presence of intron in RCY1/RPP8 genes is indispensable for higher level of RCY1/RPP8 protein accumulation. Those findings regarding pathogen recognition specificity and the role of intron for the resistance to pathogens will give new insight to understand the plant immune system against pathogen infection.
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