Roles of chromatin remodeling genes in the pathogenicity of Magnaporthe oryzae

• Project/Area Number: 22380031
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Plant pathology
• Research Institution: Kobe University
• Principal Investigator: NAKYASHIKI Hitoshi 神戸大学, 大学院・農学研究科, 教授 (50252804)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥16,380,000 (Direct Cost: ¥12,600,000、Indirect Cost: ¥3,780,000); Fiscal Year 2012: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000); Fiscal Year 2011: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000); Fiscal Year 2010: ¥9,620,000 (Direct Cost: ¥7,400,000、Indirect Cost: ¥2,220,000)
• Keywords: いもち病菌 / クロマチンリモデリング / 病原性 / ヒストンメチルトランスフェラーゼ

Research Abstract

We constructed knock-out mutants of seven histone lysine methyltransferase (HMT) genes in the fungus Magnaporthe oryzae. Phenotypic analysis revealed that one of the HMT genes, MoHMT4, which catalyzes methylation of histone H3 lysine 4, had the greatest impact on pathogenicity-related morphogenesis, signal perception and virulence. ChIP-seq analysis was performed to reveal the genome-wide changes of H3K4 methylation during infection of M. oryzae.

Research Products

• [Journal Article] Appressorium-localized NADPH oxidase B is essential for aggressiveness and pathogenicity in the host-specific, toxin-producing fungus Alternaria alternata Japanese pear pathotype. (2013)
  • Author(s): Morita Y
  • Journal Title: Mol Plant Pathol Volume: 14. Issue: 4 Pages: 365-378
  • DOI: 10.1111/mpp.12013
  • Peer Reviewed
• [Journal Article] Implication of an aldehyde dehydrogenase gene and a phosphinothricin N-Acetyltransferase gene in the diversity of Pseudomonas cichorii virulence. (2012)
  • Author(s): Tanaka, M. , Wali, U.M., Nakayashiki, H., Fukuda, T. , Mizumoto, H., Ohnishi, K., Kiba, A., Hikichi, Y.
  • Journal Title: Genes Volume: 3 Pages: 62-80
  • Peer Reviewed
• [Journal Article] Cellulases belonging to glycoside hydrolase families 6 and 7 contribute to the virulence of Magnaporthe oryzae. (2012)
  • Author(s): Vu, V.B., Itoh, K., Nguyen, Q.B. and Nakayashiki, H.
  • Journal Title: Mol. Plant-Microbe Interact Volume: 25 Pages: 1135-1141
  • Peer Reviewed
• [Journal Article] Molecular cloning of the apoptosis-related calcium-binding protein AsALG-2 in Avena sativa. (2012)
  • Author(s): Hoat,T.X., Nakayashiki,H., Tosa,Y. and Mayama,S.
  • Journal Title: Mol. Plant Pathol Volume: 14 Pages: 222-9
  • Peer Reviewed
• [Journal Article] Appressorium-localized NADPH oxidase B is essential for aggressiveness and pathogenicity in the host-specific toxin-producing fungus Alternaria alternataJapanese pear pathotype. (2012)
  • Author(s): Morita, Y., Hyon, G.-S., Hosogi, N., Miyata, N., Nakayashiki, H., Muranaka, Y., Inada, N., Park, P., and Ikeda, K.
  • Journal Title: Mol. Plant Pathol. Volume: 14 Pages: 365-78
  • Peer Reviewed
• [Journal Article] Cellulases belonging to glycoside hydrolase families 6 and 7 contribute to the virulence of Magnaporthe oryzae. (2012)
  • Author(s): Van Vu Ba
  • Journal Title: Mol Plant Microbe Interact Volume: 25 Issue: 9 Pages: 1135-1141
  • DOI: 10.1094/mpmi-02-12-0043-r
  • Peer Reviewed
• [Journal Article] Molecular cloning of the apoptosis-related calcium-binding protein AsALG-2 in Avena sativa. (2012)
  • Author(s): Hoat T.X.
  • Journal Title: Mol Plant Pathol Volume: 14 Issue: 3 Pages: 222-229
  • DOI: 10.1111/j.1364-3703.2012.00844.x
  • Peer Reviewed
• [Journal Article] Novel vectors for retrotransposon-induced gene silencing in Magnaporthe oryzae. (2011)
  • Author(s): Vu, V.B., Takino, M., Murata, T., Nakayashiki, H.
  • Journal Title: J. Gen. Plant Pathol. Volume: 77 Pages: 147-151
  • NAID: 10029720990
  • Peer Reviewed
• [Journal Article] In situ localization of avenanthramide A and its biosynthetic enzyme in oat leaves infected with crown rust fungus, Puccinia coronata f. sp. avenae. Physiol. (2011)
  • Author(s): Uchihashi, K., Nakayashiki, H., Okamura, K., Ishihara, A., Tosa, Y., Park, P., and Mayama, S.
  • Journal Title: Mol. Plant Pathol. Volume: 76 Pages: 173-181
  • Peer Reviewed
• [Journal Article] Multiple translocation of the AVR-Pita effector gene among chromosomes of the rice blast fungus Magnaporthe oryzae and related species. (2011)
  • Author(s): Chuma, I., Isobe, C., Hotta, Y., Ibaragi, K., Futamata, N., Kusaba M, Yoshida K, Terauchi R, Fujita Y, Nakayashiki, H., Valent, B., Tosa, Y.
  • Journal Title: PLoS Pathog. Volume: 7
  • Peer Reviewed
• [Journal Article] The Trickster in the genome: contribution and control of transposable elements. (2011)
  • Author(s): Nakayashiki, H.
  • Journal Title: Genes Cells. Volume: 16 Pages: 827-841
  • Peer Reviewed
• [Journal Article] Simultaneous silencing of endo-β-1,4 xylanase genes reveals their roles in the virulence of Magnaporthe oryzae. (2011)
  • Author(s): Nguyen, Q.B., Itoh, K., Van Vu, B., Tosa, Y., Nakayashiki, H.
  • Journal Title: Mol. Microbiol Volume: 81 Pages: 1008-1019
  • Peer Reviewed
• [Journal Article] Novel vectors for retrotransposon-induced gene silencing in Magnaporthe oryzae (2011)
  • Author(s): Vu, et al
  • Journal Title: Journal of General Plant Pathology Volume: 77 Issue: 3 Pages: 147-151
  • DOI: 10.1007/s10327-011-0302-5
  • Peer Reviewed
• [Journal Article] Simultaneous silencing of endo-β-1,4 xylanase genes reveals their roles in the virulence of Magnaporthe oryzae (2011)
  • Author(s): Nguyen, et al
  • Journal Title: Mol Microbiol Volume: 81 Issue: 4 Pages: 1008-1019
  • DOI: 10.1111/j.1365-2958.2011.07746.x
  • Peer Reviewed
• [Journal Article] Multiple translocation of the AVR-Pita effector gene among chromosomes of the rice blast fungus Magnaporthe oryzae and related species (2011)
  • Author(s): Chuma, et al
  • Journal Title: PloS Pathogens Volume: e1002147(web) Issue: 7 Pages: 1020147-1020147
  • DOI: 10.1371/journal.ppat.1002147
  • Peer Reviewed
• [Journal Article] Novel vectors for retrotransposon-induced gene silencing in Magnaporthe oryzae (2011)
  • Author(s): Vu, et al.
  • Journal Title: Journal of General Plant Pathology Volume: (In press)
  • Peer Reviewed
• [Journal Article] Evolution of the Eleusinesubgroup of Pyricularia oryzae inferred from rearrangement at the Pwl1 locus. (2010)
  • Author(s): Tanaka, M., Hyon, G., Murata, T., Nakayashiki, H.,and Tosa, Y.
  • Journal Title: Mol. Plant-Microbe Interact. Volume: 23 Pages: 771-783
  • Peer Reviewed
• [Journal Article] Methods to study PAMP-triggered immunity using tomato and Nicotiana benthamiana. (2010)
  • Author(s): Nguyen, H.P., Chakravarthy, S., Velasquez, A.C., McLane, H.L., Zeng, L., Nakayashiki, H., Park, D.H., Collmer, A., and Martin, G.B.
  • Journal Title: Mol Plant Microbe Interact Volume: 23 Pages: 991-999
  • Peer Reviewed
• [Journal Article] Induction of chlorosis, ROs generation and cell death by a toxin isolated from Pyricularia oryzae. (2010)
  • Author(s): Tsurushima, T., Minami, Y., Miyagawa, H., Nakayashiki, H., Tosa ,Y., and Mayama, S.
  • Journal Title: Biosci Biotechnol Biochem Volume: 74 Pages: 2220-2225
  • NAID: 10027561350
  • Peer Reviewed
• [Presentation] ヒストンメチル基転移酵素群はイネ科植物いもち病菌の感染において重要な役割を果たす (2013)
  • Author(s): Pham, T.H.K. ・ Vu, V.B. ・ Nguyen, B.Q. ・ 池田健一・中屋敷均
  • Organizer: 平成 25 年度日本植物病理学会講演要旨集 p65
  • Place of Presentation: 岐阜大学
  • Year and Date: 2013-03-29
• [Presentation] ヒストンメチル基転移酵素群はイネ科植物いもち病菌の感染において重要な役割を果たす (2013)
  • Author(s): Pham Thi Minh Kieu
  • Organizer: 日本植物病理学会大会
  • Place of Presentation: 岐阜
• [Presentation] Roles of histone lysine methyltransferases in the pathogenicity of Magnaporthe oryzae. (2012)
  • Author(s): Pham, T.H.K., Nguyen, B.Q., Vu, V.B., and Nakayashiki, H.
  • Organizer: XV International Congress on Molecular Plant-Microbe Interanctions (Kyoto International Congress Center, Kyoto, July-August).
  • Place of Presentation: 京都国際会議場
  • Year and Date: 2012-07-30
• [Presentation] Hitone methyltransferase genes play roles in infection-related morphogenesis of Magnaporthe oryzae. (2012)
  • Author(s): Pham, T.H.K., Nguyen, B.Q., Vu, V.B., and Nakayashiki, H.
  • Organizer: 平成 24 年度日本植物病理学会大会
  • Place of Presentation: 福岡国際会議場
  • Year and Date: 2012-03-28
• [Presentation] Histone Methyltransferase genes play roles in infection-related morphogenesis of Magnaporthe oryzae (2012)
  • Author(s): Pham, et al
  • Organizer: 日本植物病理学会
  • Place of Presentation: (福岡県)福岡国際会議場
  • Year and Date: 2012-03-28
• [Presentation] Roles of histone lysine methyltransferase in the pathogencicity of Magnaporthe oryzae (2012)
  • Author(s): Pham Thi Minh Kieu
  • Organizer: IS-MPMI
  • Place of Presentation: 京都
• [Presentation] Histone methyltransferase genes play rles in infection-related morphogenesis of Magnaporthe oryzae (2012)
  • Author(s): Pham Thi Minh Kieu
  • Organizer: 日本植物病理学会関西部会
  • Place of Presentation: 鳥取
• [Presentation] Roles of Histone Methyltransferase genes in infection processes of Magnaporthe oryzae. (2011)
  • Author(s): Pham, TMK, Nguyen, QB., Vu VB, Nakayashiki, H.
  • Organizer: 平成 23 年度日本植物病理学会関西部会要旨集 p36
  • Place of Presentation: 香川
  • Year and Date: 2011-10-01
• [Presentation] Building Blocks 法によるイネ科植物いもち病菌細胞壁加水分解酵素群の機能解析. (2011)
  • Author(s): 伊藤賢司・Quoc Bao Nguyen・河南裕美・村部知里・土佐幸雄・中屋敷均
  • Organizer: 平成 23 年度日本植物病理学会関西部会要旨集 p36
  • Place of Presentation: 香川
  • Year and Date: 2011-10-01
• [Presentation] Roles of Histone Methyltransferase genes in infection processes of Magnaporthe oryzae (2011)
  • Author(s): Pham, et al
  • Organizer: 日本植物病理学会関西部会
  • Place of Presentation: (香川県)サンポートホール高松
  • Year and Date: 2011-10-01
• [Presentation] 細胞壁加水分解酵素群はイネ科植物いもち病菌による角皮侵入に寄与している. (2011)
  • Author(s): 伊藤賢司・Quoc Bao Nguyen・土佐幸雄・中屋敷均
  • Organizer: 平成 23 年度日本植物病理学会講演要旨集 p56
  • Place of Presentation: 東京農工大学
  • Year and Date: 2011-03-28
• [Remarks]
  • URL: http://www.ans.kobe-u.ac.jp/jyukensei/e-bio/guide/structure.html
• [Remarks] 細胞機能構造学
  • URL: http://www.ans.kobe-u.ac.jp/jyukensei/e-bio/guide/structure.html