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Molecular Mechanism of Insect Metamorphosis: Action of Insulin-related Peptide

Research Project

Project/Area Number 22380034
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Applied entomology
Research InstitutionKanazawa University

Principal Investigator

IWAMI Masafumi  金沢大学, 自然システム学系, 教授 (40193768)

Co-Investigator(Kenkyū-buntansha) KIYA Taketoshi  金沢大学, 自然システム学系, 特任助教 (90532309)
Project Period (FY) 2010-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥18,070,000 (Direct Cost: ¥13,900,000、Indirect Cost: ¥4,170,000)
Fiscal Year 2014: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2013: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2012: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2011: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2010: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Keywords昆虫 / 脱皮・変態 / 発生・分化 / 脳・神経 / ホルモン / 昆虫変態 / インスリン / エクジソン / カイコガ / 血糖調節 / 個体発育 / 脳 / 神経 / スクロース / マルトース / プロインスリンCペプチド / 代謝調節 / 個体発育・寿命
Outline of Final Research Achievements

Insect brain plays central role during the development by orchestrating hormone production and processing information of internal and external factors. An insulin family peptide, bombyxin, is one of the hormones regulating growth, metabolism, and reproduction, but its function still remains obscure. (1) We analyzed the full silkmoth genome and identified five novel bombyxin families with their genomic organization and chromosomal location. (2) We indicated that the dietary carbohydrates hydrolyzing activity remained high throughout the last larval period and decreased to negligible levels until the pupal period and that the activity is developmentally regulated by ecdysone.
To elucidate the brain function at the neural circuit level, we intended to visualize neural circuit underlying sex pheromone-induced innate behavior. (3) We established strains in which we could visualize neural projections and cell nucleus positions that were reliably labeled with GFP after pheromone stimulation.

Report

(6 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Annual Research Report
  • 2012 Annual Research Report
  • 2011 Annual Research Report
  • 2010 Annual Research Report
  • Research Products

    (50 results)

All 2014 2013 2012 2011 2010 Other

All Journal Article (10 results) (of which Peer Reviewed: 10 results,  Open Access: 1 results,  Acknowledgement Compliant: 1 results) Presentation (30 results) Book (1 results) Remarks (9 results)

  • [Journal Article] Establishment of tools for neurogenetic analysis of sexual behavior in the silkmoth, Bombyx mori.2014

    • Author(s)
      Taketoshi Kiya, Koudai Morishita, Keiro Uchino, Masafumi Iwami, and Hideki Sezutsu
    • Journal Title

      PLoS One

      Volume: 9 (11) Issue: 11 Pages: e113156-e113156

    • DOI

      10.1371/journal.pone.0113156

    • NAID

      120005537889

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Visualization of neural activity in insect brains using a conserved immediate early gene, Hr38.2013

    • Author(s)
      Nozomi Fujita, Takumi Nishiuchi, Makoto Sato, Masafumi Iwami, and Taketoshi Kiya
    • Journal Title

      Current Biology

      Volume: 23 Issue: 20 Pages: 2063-2070

    • DOI

      10.1016/j.cub.2013.08.051

    • NAID

      120005349182

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Identification of 20-hydroxyecdysone-inducible genes from larval brain of the silkworm, Bombyx mori, and their expression analysis.2012

    • Author(s)
      Anuradha Roy
    • Journal Title

      Zoological Science

      Volume: 29 Issue: 5 Pages: 333-339

    • DOI

      10.2108/zsj.29.333

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Hormonal regulation of the death commitment in programmed cell death of the silkworm anterior silk glands.2012

    • Author(s)
      Hiroto Matsui
    • Journal Title

      Journal of Insect Physiology

      Volume: 58 Issue: 12 Pages: 1575-1581

    • DOI

      10.1016/j.jinsphys.2012.09.012

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Glucose oxidase prevents programmed cell death of the silkworm anterior silk gland through hydrogen peroxide production2011

    • Author(s)
      Hiroto Matsui, Motonori Kakei, Masafumi Iwami, Sho Sakurai
    • Journal Title

      FEBS Journal

      Volume: Vol.278 Issue: 5 Pages: 776-785

    • DOI

      10.1111/j.1742-4658.2010.07996.x

    • NAID

      120002924883

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Identification and characterization of a novel nuclear noncoding RNA, Fben-1, which is preferentially expressed in the higher brain center of the female silkworm moth, Bombyx mori2011

    • Author(s)
      Syusaku Taguchi, Masafumi Iwami, Taketoshi Kiya
    • Journal Title

      Neuroscience Letters

      Volume: Vol.496 Issue: 3 Pages: 176-180

    • DOI

      10.1016/j.neulet.2011.04.011

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Identification of novel bombyxin genes from the genome of the silkmoth Bombyx mori and analysis of their exoression2011

    • Author(s)
      Abu F M Aslam, Taketoshi Kiya, Kazuei Mita, Masafumi Iwami
    • Journal Title

      Zoological Science

      Volume: Vol.28 Issue: 8 Pages: 609-616

    • DOI

      10.2108/zsj.28.609

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Steroidal regulation of hydrolyzing activity of the dietary carbohydrates in the silkworm, Bombyx mori2011

    • Author(s)
      Takumi Suzuki, Sho Sakurai, Masafumi Iwami
    • Journal Title

      Journal of Insect Physiology

      Volume: Vol.57 Issue: 9 Pages: 1282-1289

    • DOI

      10.1016/j.jinsphys.2011.06.003

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Identification and expression analysis of nervous wreck, which is preferentially expressed in the brain of the male silkworm moth, Bombyx2011

    • Author(s)
      Taketoshi Kiya, Masafumi Iwami
    • Journal Title

      Insect Molecular Biology

      Volume: Vol.20 Issue: 5 Pages: 667-674

    • DOI

      10.1111/j.1365-2583.2011.01096.x

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Juvenile hormone delays the initiation of rectal sac distention by disrupting ecdysteroid action in the silkworm, Bombyx mori.2010

    • Author(s)
      Takumi Suzuki, Sho Sakurai, Masafumi Iwami
    • Journal Title

      Pesticide Biochemistry and Physiology

      Volume: 97 Pages: 199-203

    • NAID

      120002029021

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Presentation] 神経活動依存的に発現する遺伝子Hr38を用いたショウジョウバエ脳においてメスに反応する神経細胞の検出2014

    • Author(s)
      木矢剛智, 佐藤優希, 岩見雅史
    • Organizer
      日本分子生物学会第38回年会
    • Place of Presentation
      パシフィコ横浜(神奈川県横浜市)
    • Year and Date
      2014-11-25 – 2014-11-27
    • Related Report
      2014 Annual Research Report
  • [Presentation] Hr38の神経活動依存的発現を利用した、カイコガの脳において性フェロモンに応答する神経回路の可視化2014

    • Author(s)
      山田裕果, 原千穂, 内野恵郎, 櫻井健志, 神崎亮平, 瀬筒秀樹, 岩見雅史, 木矢剛智
    • Organizer
      日本分子生物学会第38回年会
    • Place of Presentation
      パシフィコ横浜(神奈川県横浜市)
    • Year and Date
      2014-11-25 – 2014-11-27
    • Related Report
      2014 Annual Research Report
  • [Presentation] 初期応答遺伝子Hr38はショウジョウバエの長期記憶において重要な役割を果たす2014

    • Author(s)
      箕口昌杜, 大村知世, 長田有加, 佐藤翔馬, 坂井貴臣, 岩見雅史, 木矢剛智
    • Organizer
      日本分子生物学会第38回年会
    • Place of Presentation
      パシフィコ横浜(神奈川県横浜市)
    • Year and Date
      2014-11-25 – 2014-11-27
    • Related Report
      2014 Annual Research Report
  • [Presentation] Detection of neurons that respond to female in the brain of male Drosophila, using a neural activity marker gene, Hr38.2014

    • Author(s)
      Taketoshi Kiya, Makoto Sato, Masafumi Iwami
    • Organizer
      The 11th Japanese Drosophila Research Conference
    • Place of Presentation
      金沢歌劇座(石川県金沢市)
    • Year and Date
      2014-06-04 – 2014-06-06
    • Related Report
      2014 Annual Research Report
  • [Presentation] 新規な初期応答遺伝子Hr38を用いたショウジョウバエ脳においてメスに反応する神経細胞の検出2013

    • Author(s)
      木矢剛智, 佐藤優希, 岩見雅史
    • Organizer
      日本神経科学会大会第36回大会
    • Place of Presentation
      国立京都国際会館(京都府京都市)
    • Related Report
      2013 Annual Research Report
  • [Presentation] Identification of Hr38 as a conserved neural activity-induced gene in insect brains: its application as a marker for neural activity and implication for neural activity-dependent modification of ecdysone signaling in the brain.2013

    • Author(s)
      Nozomi Fujita, Hiroki Yamahana, Yuka Nagata, Takumi Nishiuchi, Makoto Sato, Masafumi Iwami, Taketoshi Kiya
    • Organizer
      Insect Hormones (19th Ecdysone) International Workshop
    • Place of Presentation
      University of Minnesota (Minnesota, USA)
    • Related Report
      2013 Annual Research Report
  • [Presentation] ミツバチの脳においてエクダイソン・シグナルは長期記憶の形成を促進する2013

    • Author(s)
      山端宏葵, 岩見雅史, 木矢剛智
    • Organizer
      日本動物学会第84回大会
    • Place of Presentation
      岡山大学(岡山県岡山市)
    • Related Report
      2013 Annual Research Report
  • [Presentation] 遺伝子組み換えカイコガを用いた幼若ホルモン生合成制御機構の解析2013

    • Author(s)
      田口周作, 内野恵郎, 瀬筒秀樹, 岩見雅史, 木矢剛智
    • Organizer
      日本動物学会第84回大会
    • Place of Presentation
      岡山大学(岡山県岡山市)
    • Related Report
      2013 Annual Research Report
  • [Presentation] 初期応答遺伝子Hr38を用いたショウジョウバエ脳においてメスに反応する神経回路の同定2013

    • Author(s)
      木矢剛智, 佐藤優希, 岩見雅史
    • Organizer
      日本動物学会第84回大会
    • Place of Presentation
      岡山大学(岡山県岡山市)
    • Related Report
      2013 Annual Research Report
  • [Presentation] 神経活動依存的に発現する遺伝子Hr38を用いたショウジョウバエ脳においてメスに反応する神経細胞の検出2013

    • Author(s)
      佐藤優希, 岩見雅史, 木矢剛智
    • Organizer
      日本分子生物学会第36回年会
    • Place of Presentation
      ポートピアホテル(兵庫県神戸市)
    • Related Report
      2013 Annual Research Report
  • [Presentation] ミツバチの脳においてエクジステロイド・シグナルは長期記憶の形成を促進する2013

    • Author(s)
      山端宏葵, 岩見雅史, 木矢剛智
    • Organizer
      日本分子生物学会第36回年会
    • Place of Presentation
      ポートピアホテル(兵庫県神戸市)
    • Related Report
      2013 Annual Research Report
  • [Presentation] 新規な初期応答遺伝子Hr38の活動依存的発現を利用した、カイコガの脳において性フェロモンに応答する神経回路の可視化法と機能解析法の開発2013

    • Author(s)
      原千穂, 森下広大, 田口周作, 内野恵郎, 櫻井健志, 神崎亮平, 岩見雅史, 瀬筒秀樹, 木矢剛智
    • Organizer
      日本分子生物学会第36回年会
    • Place of Presentation
      ポートピアホテル(兵庫県神戸市)
    • Related Report
      2013 Annual Research Report
  • [Presentation] 神経活動依存的に発現する遺伝子を用いた昆虫脳の神経回路可視化法の開発2011

    • Author(s)
      木矢剛智, 藤田望, 西内巧, 佐藤純, 岩見雅史
    • Organizer
      日本分子生物学会第34回年会
    • Place of Presentation
      パシフィコ横浜(神奈川県)
    • Year and Date
      2011-12-13
    • Related Report
      2011 Annual Research Report
  • [Presentation] 新規な神経活動依存的遺伝子を用いた昆虫脳の神経回路可視化法の開発2011

    • Author(s)
      木矢剛智, 藤田望, 西内巧, 佐藤純, 岩見雅史
    • Organizer
      日本動物学会第82回大会
    • Place of Presentation
      大雪クリスタルホール(北海道)
    • Year and Date
      2011-09-21
    • Related Report
      2011 Annual Research Report
  • [Presentation] 新規な神経活動依存的遺伝子を用いたカイコガ及びショウジョウバエの性フェロモン神経回路の可視化2011

    • Author(s)
      木矢剛智, 藤田望, 西内巧, 佐藤純, 岩見雅史
    • Organizer
      日本神経科学大会第34回大会
    • Place of Presentation
      パシフィコ横浜(神奈川県)
    • Year and Date
      2011-09-16
    • Related Report
      2011 Annual Research Report
  • [Presentation] エクジステロイドによる糖分解酵素活性の制御2011

    • Author(s)
      鈴木匠, 桜井勝, 岩見雅史
    • Organizer
      日本分子生物学会第11回春季シンポジウム
    • Place of Presentation
      石川県立音楽堂(石川県)
    • Year and Date
      2011-05-26
    • Related Report
      2011 Annual Research Report
  • [Presentation] 神経活動依存的に発現する遺伝子を用いた昆虫脳の神経回路可視化法の開発2011

    • Author(s)
      木矢剛智, 藤田望, 西内巧, 佐藤純, 岩見雅史
    • Organizer
      日本分子生物学会第11回春季シンポジウム
    • Place of Presentation
      石川県立音楽堂(石川県)
    • Year and Date
      2011-05-26
    • Related Report
      2011 Annual Research Report
  • [Presentation] 幼若ホルモン生合成期にアラタ体、側心体で発現量増加する新規遺伝子の同定と解析2010

    • Author(s)
      田口周作, 岩見雅史, 木矢剛智
    • Organizer
      日本分子生物学会第33回年会(日本生化学会第83回大会合同大会)
    • Place of Presentation
      神戸市
    • Year and Date
      2010-12-08
    • Related Report
      2010 Annual Research Report
  • [Presentation] 神経活動依存的に発現する遺伝子を用いた昆虫脳の神経回路可視化法の開発2010

    • Author(s)
      藤田望, 西内巧, 岩見雅史, 木矢剛智
    • Organizer
      日本分子生物学会第33回年会(日本生化学会第83回大会合同大会)
    • Place of Presentation
      神戸市
    • Year and Date
      2010-12-07
    • Related Report
      2010 Annual Research Report
  • [Presentation] 初期応答遺伝子を用いたカイコガ性フェロモン情報処理回路の探索2010

    • Author(s)
      藤田望, 西内巧, 岩見雅史, 木矢剛智
    • Organizer
      日本動物学会第81回大会
    • Place of Presentation
      東京都
    • Year and Date
      2010-09-25
    • Related Report
      2010 Annual Research Report
  • [Presentation] カイコガ幼虫における中腸グルコシダーゼ活性の制御2010

    • Author(s)
      鈴木匠, 桜弁勝, 岩見雅史
    • Organizer
      日本動物学会第81回大会
    • Place of Presentation
      東京都
    • Year and Date
      2010-09-23
    • Related Report
      2010 Annual Research Report
  • [Presentation] 幼若ホルモン生合成期にアラタ体、側心体で発現燈増加する遺伝子の同定2010

    • Author(s)
      田口周作, 岩見雅史, 木矢剛智
    • Organizer
      日本動物学会第81回大会
    • Place of Presentation
      東京都
    • Year and Date
      2010-09-23
    • Related Report
      2010 Annual Research Report
  • [Presentation] Identification of novel bombyxin genes from the genome of the silkmoth Bombyx mori.2010

    • Author(s)
      Aslam AFM, Kiya T, Higuchi T, Sakurai S, Mita S, Iwami M.
    • Organizer
      4th Annual Arthoropod Genomics Symposium
    • Place of Presentation
      Kansas City, USA
    • Year and Date
      2010-06-11
    • Related Report
      2010 Annual Research Report
  • [Presentation] カイコガの性行動時の運動パターンを生成する神経基盤の解明

    • Author(s)
      森下 広大
    • Organizer
      日本動物学会第83回大会
    • Place of Presentation
      大阪大学(大阪府)
    • Related Report
      2012 Annual Research Report
  • [Presentation] 熱遺伝学的手法によるカイコガの神経活動および行動の操作

    • Author(s)
      木矢 剛智
    • Organizer
      日本動物学会第83回大会
    • Place of Presentation
      大阪大学(大阪府)
    • Related Report
      2012 Annual Research Report
  • [Presentation] 新規な神経活動依存的遺伝子M8は昆虫で広く保存された有用な神経活動マーカーである

    • Author(s)
      木矢 剛智
    • Organizer
      日本神経科学会大会第35回大会
    • Place of Presentation
      名古屋国際会議場(愛知県)
    • Related Report
      2012 Annual Research Report
  • [Presentation] Functional analysis of a novel immediate early gene, Hr38, in the long-term courtship memory in Drosophila

    • Author(s)
      Tomoyo Ohmura
    • Organizer
      日本ショウジョウバエ研究会第10回研究集会
    • Place of Presentation
      慈恵医大(東京都)
    • Related Report
      2012 Annual Research Report
  • [Presentation] Development of activity-dependent neural tracing methods in the brain of fruit fly, Drosophila melanogaster

    • Author(s)
      Yuki Sato
    • Organizer
      日本ショウジョウバエ研究会第10回研究集会
    • Place of Presentation
      慈恵医大(東京都)
    • Related Report
      2012 Annual Research Report
  • [Presentation] Neural activity visualization in insect brains by a conserved immediate early gene, Hr38

    • Author(s)
      Taketoshi Kiya
    • Organizer
      日本ショウジョウバエ研究会第10回研究集会
    • Place of Presentation
      慈恵医大(東京都)
    • Related Report
      2012 Annual Research Report
  • [Presentation] 昆虫で広く保存された新規な初期応答遺伝子Hr38を用いた活動依存的な神経回路の可視化

    • Author(s)
      木矢 剛智
    • Organizer
      日本分子生物学会第35回年会
    • Place of Presentation
      福岡国際会議場(福岡県)
    • Related Report
      2012 Annual Research Report
  • [Book] Hemolymph proteins and functional peptides: Recent advances in insects and other arthropods (第9章担当)2012

    • Author(s)
      Muhammad Tufail
    • Publisher
      Bentham Science Publishers, Oak Park, IL, USA
    • Related Report
      2012 Annual Research Report
  • [Remarks] Masafumi IWAMI

    • URL

      http://bombyxin.umin.jp

    • Related Report
      2014 Annual Research Report
  • [Remarks] Masafumi IWAMI

    • URL

      http://biology.w3.kanazawa-u.ac.jp/index.html

    • Related Report
      2014 Annual Research Report
  • [Remarks] 研究者情報

    • URL

      http://ridb.kanazawa-u.ac.jp/public/detail.php?id=3156&page=1&org2_cd=341200

    • Related Report
      2014 Annual Research Report
  • [Remarks] Masafumi IWAMI

    • URL

      http://bombyxin.umin.jp

    • Related Report
      2013 Annual Research Report
  • [Remarks] Masafumi IWAMI

    • URL

      http://biology.w3.kanazawa-u.ac.jp/index.html

    • Related Report
      2013 Annual Research Report
  • [Remarks] 研究者情報

    • URL

      http://ridb.kanazawa-u.ac.jp/public/detail.php?id=3156&page=1&org2_cd=341200

    • Related Report
      2013 Annual Research Report
  • [Remarks] 金沢大学研究者情報

    • URL

      http://ridb.kanazawa-u.ac.jp/public/detail.php?id=3156&page=1&org2_cd=341200

    • Related Report
      2012 Annual Research Report
  • [Remarks] 岩見雅史 個人のホームページ

    • URL

      http://biology.w3.kanazawa-u.ac.jp

    • Related Report
      2012 Annual Research Report
  • [Remarks] 岩見雅史 個人のホームページ

    • URL

      http://bombyxin.umin.jp

    • Related Report
      2012 Annual Research Report

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Published: 2010-08-23   Modified: 2019-07-29  

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