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Evolution of odorant receptor genes in Ostrinia moths and its involvement in male response to sex pheromone.

Research Project

Project/Area Number 22380040
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Applied entomology
Research InstitutionNational Institute of Agrobiological Sciences

Principal Investigator

YASUKOCHI Yuji  独立行政法人農業生物資源研究所, 昆虫ゲノム研究ユニット, 主任研究員 (50355723)

Co-Investigator(Kenkyū-buntansha) ISHIKAWA Yukio  東京大学, 大学院・農学生命科学研究科, 教授 (60125987)
SAHARA Ken  岩手大学, 農学部, 教授 (30241368)
Project Period (FY) 2010 – 2012
Project Status Completed (Fiscal Year 2012)
Budget Amount *help
¥18,720,000 (Direct Cost: ¥14,400,000、Indirect Cost: ¥4,320,000)
Fiscal Year 2012: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2011: ¥7,150,000 (Direct Cost: ¥5,500,000、Indirect Cost: ¥1,650,000)
Fiscal Year 2010: ¥7,020,000 (Direct Cost: ¥5,400,000、Indirect Cost: ¥1,620,000)
Keywords性フェロモン / アワノメイガ / 嗅覚受容体 / 遺伝子重複 / BAC / フォスミド / 不飽和化酵素
Research Abstract

Sex pheromone plays a critical role in species-specific mating behaviors, however, little is known about what changes occur in genes responsible for the behaviors. Thus, we performed a comparative analysis of genes associated with sex pheromone response and synthesis between Ostrinia furnacalis and Ostrinia latipennis. We constructed genomic fosmid libraries of the species, and generated PCR-based screening system for them. Then, we isolated clones that contain genes encoding receptors, binding proteins and desaturase of sex pheromone, and determined genomic sequences (330kb for O. furnaclis and 265kb for O. latipennis)

Report

(4 results)
  • 2012 Annual Research Report   Final Research Report ( PDF )
  • 2011 Annual Research Report
  • 2010 Annual Research Report
  • Research Products

    (12 results)

All 2013 2012 2011 2010

All Journal Article (3 results) (of which Peer Reviewed: 3 results) Presentation (9 results)

  • [Journal Article] Sex-linked pheromone receptor genes of the European corn borer, Ostrinia nubilalis, are in tandem arrays2011

    • Author(s)
      Yasukochi Y, Miura N, Nakano R, Sahara K, Ishikawa Y
    • Journal Title

      PLoS ONE

      Volume: 6 Issue: 4 Pages: 18843-18843

    • DOI

      10.1371/journal.pone.0018843

    • Related Report
      2012 Final Research Report 2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Isolation of BAC clones containing conserved genes from libraries of three distantly related moths: A useful resource for comparative genomics of Lepidoptera2011

    • Author(s)
      Yasukochi Y, Tanaka-Okuyama M, Kamimura M, Nakano R, Naito Y, Ishikawa Y, Sahara K
    • Journal Title

      Journal of Biomedicine and Biotechnology

      Volume: 2011 Issue: 1 Pages: 165894-165894

    • DOI

      10.1155/2011/165894

    • NAID

      120003043350

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Isolation of BAC clones containing conserved genes from libraries of three distantly related moths : A useful resource for comparative genomics of Lepidoptera2011

    • Author(s)
      Yasukochi Y, Tanaka-Okuyama M, Kamimura M, Nakano R, Naito Y, Ishikawa Y, Sahara K
    • Journal Title

      Journal of Biomedicine and Biotechnology

      Volume: 2011 Pages: 165894-165894

    • NAID

      120003043350

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Presentation] アワノメイガ類の性フェロモン生合成に関与する不飽和化酵素遺伝子の種間比較2013

    • Author(s)
      藤井毅・安河内祐二・戎〓・中野亮・石川幸男
    • Organizer
      第57回日本応用動物昆虫学会大会
    • Place of Presentation
      藤沢市
    • Year and Date
      2013-03-28
    • Related Report
      2012 Final Research Report
  • [Presentation] アワノメイガとウスジロキノメイガの性フェロモン受容体遺伝子クラスターの構造解明2013

    • Author(s)
      安河内祐二・松尾隆嗣・石川幸男
    • Organizer
      日本蚕糸学会第83回大会
    • Place of Presentation
      つくば市
    • Year and Date
      2013-03-19
    • Related Report
      2012 Final Research Report
  • [Presentation] アワノメイガとウスジロキノメイガの性フェロモン受容体遺伝子クラスターの構造解明2013

    • Author(s)
      安河内祐二
    • Organizer
      日本蚕糸学会第83回大会
    • Place of Presentation
      茨城県つくば市
    • Related Report
      2012 Annual Research Report
  • [Presentation] アワノメイガ類の性フェロモン生合成に関与する不飽和化酵素遺伝子の種間比較2013

    • Author(s)
      藤井毅
    • Organizer
      第57回日本応用動物昆虫学会大会
    • Place of Presentation
      神奈川県藤沢市
    • Related Report
      2012 Annual Research Report
  • [Presentation] アワノメイガとウスジロキノメイガのフォスミドライブラリーの作製と性フェロモン関連遺伝子のゲノム塩基配列決定2012

    • Author(s)
      安河内祐二・三浦奈美・藤井毅・松尾隆嗣・石川幸男
    • Organizer
      日本蚕糸学会第82回大会
    • Place of Presentation
      九州大学
    • Year and Date
      2012-03-19
    • Related Report
      2012 Final Research Report 2011 Annual Research Report
  • [Presentation] ヨーロッパアワノメイガのオス触角特異的に発現する嗅覚受容体遺伝子の重複2010

    • Author(s)
      安河内祐二・三浦奈美・佐原健・石川幸男
    • Organizer
      第33回日本分子生物学会年会・第83回日本生化学会大会合同大会
    • Place of Presentation
      神戸市
    • Year and Date
      2010-12-07
    • Related Report
      2012 Final Research Report
  • [Presentation] ヨーロッパアワノメイガのオス触角特異的に発現する嗅覚受容体遺伝子の重複2010

    • Author(s)
      安河内祐二・三浦奈美・佐原健・石川幸男
    • Organizer
      第33回日本分子生物学会年会・第83回日本生化学会大会合同大会
    • Place of Presentation
      神戸国際展示場
    • Year and Date
      2010-12-07
    • Related Report
      2010 Annual Research Report
  • [Presentation] ヨーロッパアワノメイガの触角でオス特異的に発現する嗅覚受容体遺伝子の重複2010

    • Author(s)
      安河内祐二・三浦奈美・石川幸男・佐原健
    • Organizer
      日本蚕糸学会第80回大会
    • Place of Presentation
      上田市
    • Year and Date
      2010-04-03
    • Related Report
      2012 Final Research Report
  • [Presentation] ヨーロッパアワノメイガの触角でオス特異的に発現する嗅覚受容体遺伝子の重複2010

    • Author(s)
      安河内祐二・三浦奈美・石川幸男・佐原健
    • Organizer
      日本蚕糸学会第80回大会
    • Place of Presentation
      信州大学繊維学部
    • Year and Date
      2010-04-03
    • Related Report
      2010 Annual Research Report

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Published: 2010-08-23   Modified: 2019-07-29  

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