| Project/Area Number |
22380041
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Applied entomology
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| Research Institution | National Institute of Agrobiological Sciences |
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Principal Investigator |
YAMAMOTO Kimiko 独立行政法人農業生物資源研究所, 昆虫ゲノム研究ユニット, ユニット長 (40370689)
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| Co-Investigator(Kenkyū-buntansha) |
MIYAMOTO Kazuhisa 農業生物資源研究所, 昆虫微生物機能研究ユニット, 上級研究員 (10370686)
JOURAKU Akiya 農業生物資源研究所, 昆虫ゲノム研究ユニット, 任期付研究員 (60542115)
SUETUGU Yoshitaka 農業生物資源研究所, 昆虫ゲノム研究ユニット, 主任研究員 (80533471)
NODA Hiroaki 農業生物資源研究所, 昆虫科学研究領域, 特任上級研究員 (40343991)
MITA Kazuei 農業生物資源研究所, 昆虫科学研究領域, 恃任上級研究員 (30159165)
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| Project Period (FY) |
2010 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥18,980,000 (Direct Cost: ¥14,600,000、Indirect Cost: ¥4,380,000)
Fiscal Year 2012: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2011: ¥7,150,000 (Direct Cost: ¥5,500,000、Indirect Cost: ¥1,650,000)
Fiscal Year 2010: ¥8,190,000 (Direct Cost: ¥6,300,000、Indirect Cost: ¥1,890,000)
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| Keywords | 昆虫分子生物学 / コナガゲノム / Bt菌毒素抵抗性 / コナガ / cDNA / ゲノム / Bt抵抗性 |
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| Research Abstract |
EST analysis and RNAseq were performed and more than 30,000 gene candidates were acquired. KONAGAbase that provides diamondback moth comprehensive transcriptomic and draft genomic sequences with useful annotation information was developed and opened to the public. The candidates of Bt toxin resistance related genes were found with genetic analysis. The result of gene expression profiling showed that the genes associated with medicine metabolism had the low relevance with Bt toxin resistance.
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