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Molecular mechanisms underlying nitrate-responsive gene expression in higher plants

Research Project

Project/Area Number 22380043
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Plant nutrition/Soil science
Research InstitutionThe University of Tokyo

Principal Investigator

YANAGISAWA Shuichi  東京大学, 生物生産工学研究センター, 准教授 (20222359)

Project Period (FY) 2010-04-01 – 2013-03-31
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥17,550,000 (Direct Cost: ¥13,500,000、Indirect Cost: ¥4,050,000)
Fiscal Year 2012: ¥6,630,000 (Direct Cost: ¥5,100,000、Indirect Cost: ¥1,530,000)
Fiscal Year 2011: ¥6,630,000 (Direct Cost: ¥5,100,000、Indirect Cost: ¥1,530,000)
Fiscal Year 2010: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Keywords植物 / 硝酸応答 / 硝酸シグナル / 遺伝子発現 / 転写因子 / 栄養代謝調節 / 転写制御 / 転写 / シグナル伝達 / 植物栄養
Research Abstract

In plants, nitrate is not only a major nitrogen source but also a signaling molecule that modulates the expression of a wide range of genes, including the genes that encode nitrate assimilation-related enzymes. To reveal molecular mechanisms underlying nitrate-responsive gene expression, we identified NLPs as key transcription factors controlling nitrate-responsive gene expression and nitrate-inducible plant responses in this study. We showed that the NLP transcription factors are post-translationally activated by nitrate and then promote transcription of most of nitrate-inducible genes in Arabidopsis. Furthermore, we also suggested that the NLP transcription factors play similar roles in nitrate responses in an important crop, maize.

Report

(4 results)
  • 2013 Final Research Report ( PDF )
  • 2012 Annual Research Report
  • 2011 Annual Research Report
  • 2010 Annual Research Report
  • Research Products

    (43 results)

All 2014 2013 2012 2011 2010 Other

All Journal Article (13 results) (of which Peer Reviewed: 13 results,  Open Access: 1 results) Presentation (27 results) (of which Invited: 4 results) Book (1 results) Remarks (2 results)

  • [Journal Article] An NLP-binding site in the 3' flanking region of the nitrate reductase gene confers nitrate-inducible expression in Arabidopsis2013

    • Author(s)
      Konishi, M., Yanagisawa, S.
    • Journal Title

      Soil Science and Plant Nutrition

      Volume: 59 Issue: 4 Pages: 612-620

    • DOI

      10.1080/00380768.2013.809602

    • Related Report
      2013 Final Research Report 2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The evolutionary events necessary for the emergence of symbiotic nitrogen fixation in legumes may involve a loss of nitrate responsiveness of the NIN transcription factor2013

    • Author(s)
      Suzuki, W., Konishi, M., Yanagisawa, S.
    • Journal Title

      Plant Signaling & Behavior

      Volume: 8 Issue: 10 Pages: e25975-e25975

    • DOI

      10.4161/psb.25975

    • Related Report
      2013 Final Research Report 2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Arabidopsis NIN-like transcription factors play a central role in nitrate signalling2013

    • Author(s)
      Konishi, M. and Yanagisawa, S.
    • Journal Title

      Nature Communications

      Volume: 4 Issue: 1 Pages: 1617-1617

    • DOI

      10.1038/ncomms2621

    • Related Report
      2013 Final Research Report 2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Characterization of a nitrate-inducible transcriptional repressor NIGT1 provides new insights into DNA recognition by the GARP family proteins2013

    • Author(s)
      Yanagisawa, S.
    • Journal Title

      Plant Signaling & Behavior

      Volume: 8 Issue: 6 Pages: e24447-e24447

    • DOI

      10.4161/psb.24447

    • Related Report
      2013 Final Research Report 2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A nitrate-inducible GARP family gene encodes an auto-repressible transcriptional repressor in rice2013

    • Author(s)
      Sawaki, N., Tsujimoto, R., Shigyo, M., Konishi, M., Toki, S., Fujiwara, T. and Yanagisawa, S.
    • Journal Title

      Plant & Cell Physiology

      Volume: 54 Issue: 4 Pages: 506-517

    • DOI

      10.1093/pcp/pct007

    • Related Report
      2013 Final Research Report 2011 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Involvement of PpDof1 transcriptional repressor in the nutrient condition-dependent growth control of protonemal filaments in Physcomitrella patens2012

    • Author(s)
      Sugiyama T., Ishida T., Tabei N., Shigyo M., Konishi M., Yoneyama T., and Yanagisawa S.
    • Journal Title

      Journal of Experimental Botany

      Volume: 63 Issue: 8 Pages: 3185-3197

    • DOI

      10.1093/jxb/ers042

    • Related Report
      2013 Final Research Report 2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Proteomic characterization of the greening process in rice seedlings using the MS spectral intensity-based label free method2012

    • Author(s)
      Hamamoto K., Aki T., Shigyo M., Sato S., Ishida T., Yano K., Yoneyama T., and Yanagisawa S.
    • Journal Title

      Journal of Proteome Resserach

      Volume: 11 Issue: 1 Pages: 331-347

    • DOI

      10.1021/pr200852q

    • Related Report
      2013 Final Research Report 2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Roles of the transcriptional regulation mediated by the nitrate-responsive cis-element in higher plants2012

    • Author(s)
      Mineko Konishi, Shuichi Yanagisawa
    • Journal Title

      Biochemical and Biophysical Research Communications

      Volume: 411 Pages: 708-713

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The regulatory region controlling the nitrate-responsive expression of a nitrate reductase gene, NIA1, in Arabidopsis2011

    • Author(s)
      Konishi M., and Yanagisawa S.
    • Journal Title

      Plant and Cell Physiology

      Volume: 52 Issue: 5 Pages: 824-836

    • DOI

      10.1093/pcp/pcr033

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Roles of the transcriptional regulation mediated by the nitrate-responsive cis-element in higher plants2011

    • Author(s)
      Mineko Konishi, Shuichi Yanagisawa
    • Journal Title

      Biochemical and Biophysical Research Communications

      Volume: 411 Issue: 4 Pages: 708-713

    • DOI

      10.1016/j.bbrc.2011.07.008

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] The Regulatory Region Controlling the Nitrate-responsive Expression of a Nitrate Reductase Gene, NIA1, in Arabidopsis2011

    • Author(s)
      Mineko Konishi, Shuichi Yanagisawa
    • Journal Title

      Plant and Cell Physiology

      Volume: 52 Pages: 824-836

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Characterization of plant eukaryotic translation initiation factor 6 (eIF6) genes : The essential role in embryogenesis and their differential expression in Arabidopsis and rice2010

    • Author(s)
      Kato Y., Konishi M., Shigyo M., Yoneyama T., and Yanagisawa S.
    • Journal Title

      Biochemical and Biophysical Research Communications

      Volume: 397 Pages: 673-678

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Identification of the nitrate-responsive cis-element in the Arabidopsis NIR1 promoter defines the presence of multiple cis-elements for nitrogen response2010

    • Author(s)
      Konishi M., and Yanagisawa S.
    • Journal Title

      The Plant Journal

      Volume: 63 Issue: 2 Pages: 269-282

    • DOI

      10.1111/j.1365-313x.2010.04239.x

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Presentation] 植物の硝酸応答機構研究の新展開2014

    • Author(s)
      柳澤修一、小西美稲子、鈴木渉(2014)
    • Organizer
      植物生理学会2014年度年会シンポジウム「植物の三大栄養素(N-P-K)の感知と利用の新理解」
    • Place of Presentation
      富山
    • Related Report
      2013 Final Research Report
  • [Presentation] C/N栄養応答に関与する新規ユビキチンリガーゼBTタンパク質の機能解析2014

    • Author(s)
      佐々木勇樹,安田盛貴,柳澤修一,佐藤長緒,山口淳二(2014)
    • Organizer
      植物生理学会2014年度年会
    • Place of Presentation
      富山
    • Related Report
      2013 Final Research Report
  • [Presentation] 硝酸誘導型転写因子NLPによるBTタンパク質遺伝子の発現誘導2014

    • Author(s)
      吉岡希、小西美稲子、石田哲也、柳澤修一(2014)
    • Organizer
      植物生理学会2014年度年会
    • Place of Presentation
      富山
    • Related Report
      2013 Final Research Report
  • [Presentation] 高等植物の硝酸応答を担うNLP転写因子の機能ドメインの解析2014

    • Author(s)
      小西美稲子,鈴木渉,柳澤修一
    • Organizer
      植物生理学会2014年度年会
    • Place of Presentation
      富山
    • Related Report
      2013 Final Research Report
  • [Presentation] グルコース応答性遺伝子AtNuGAP1由来の核タンパク質の胚発生への関与.2014

    • Author(s)
      石田哲也,柳澤修一
    • Organizer
      植物生理学会2014年度年会
    • Place of Presentation
      富山
    • Related Report
      2013 Final Research Report
  • [Presentation] 植物の硝酸応答機構研究の新展開2014

    • Author(s)
      柳澤修一,小西美稲子,鈴木渉
    • Organizer
      第55回日本植物生理学会年会シンポジウム「植物の三大栄養素(N-P-K)の感知と利用の新理解」
    • Place of Presentation
      富山
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Presentation] C/N栄養応答に関与する新規ユビキチンリガーゼBTタンパク質の機能解析2014

    • Author(s)
      佐々木勇樹, 安田盛貴, 柳澤修一,佐藤長緒, 山口淳二
    • Organizer
      第55回日本植物生理学会年会
    • Place of Presentation
      富山
    • Related Report
      2012 Annual Research Report
  • [Presentation] 硝酸誘導型転写因子NLPによるBTタンパク質遺伝子の発現誘導2014

    • Author(s)
      吉岡希、小西美稲子、石田哲也、柳澤修一
    • Organizer
      第55回日本植物生理学会年会
    • Place of Presentation
      富山
    • Related Report
      2012 Annual Research Report
  • [Presentation] NBP転写因子による硝酸応答の制御2013

    • Author(s)
      小西美稲子, 澤修一
    • Organizer
      第54回日本植物生理学会年会
    • Place of Presentation
      岡山
    • Year and Date
      2013-03-23
    • Related Report
      2013 Final Research Report
  • [Presentation] NIN-like proteinによる硝酸誘導性遺伝子発現の制御2013

    • Author(s)
      小西美稲子,鈴木渉,柳澤修一
    • Organizer
      日本土壌肥料学会2013年度名古屋大会
    • Place of Presentation
      名古屋
    • Related Report
      2013 Final Research Report
  • [Presentation] つくばFACEサイトのイネの葉の呼吸系の高CO2応答2013

    • Author(s)
      野口航,渡辺千尋,酒井英光,長谷川利拡,柳澤修一,寺島一郎
    • Organizer
      日本植物学会第77回大会
    • Place of Presentation
      札幌
    • Related Report
      2013 Final Research Report
  • [Presentation] The central role of NLP transcription factors in nitrate-inducible gene expression2013

    • Author(s)
      Konishi M., and Yanagisawa S.
    • Organizer
      The 24th International Conference on Arabidopsis Research (ICAR)
    • Place of Presentation
      Sydney, Australia
    • Related Report
      2013 Final Research Report
  • [Presentation] Transcriptional regulation of gibberellin biosynthesis by ethylene signaling in the growth response of submerged deepwater rice2013

    • Author(s)
      Kuroha T., Keisuke Nagai K., Ayano M., Yanagisawa S., Ashikari M.
    • Organizer
      The 21st International Conference on Plant Growth Substances IPGSA2013
    • Place of Presentation
      Shanghai, China
    • Related Report
      2013 Final Research Report
  • [Presentation] Members of the nin-like protein family are transcription factors governing nitrate-inducible gene expression2013

    • Author(s)
      Yanagisawa S
    • Organizer
      Nitrogen 2013 (2nd International Symposium on the Nitrogen Nutrition of Plants)
    • Place of Presentation
      Puerto Varas, Chile
    • Related Report
      2013 Final Research Report
  • [Presentation] Members of the nin-like protein family are transcription factors governing nitrate-inducible gene expression2013

    • Author(s)
      Yanagisawa, S.
    • Organizer
      Nitrogen 2013
    • Place of Presentation
      Puerto Varas, Chile
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Presentation] 植物転写因子を用いた植物機能の強化2012

    • Author(s)
      柳澤修一
    • Organizer
      生物生産工学研究センターシンポジウム"植物機能のバイオテクノロジー"
    • Place of Presentation
      東京
    • Year and Date
      2012-11-05
    • Related Report
      2013 Final Research Report
  • [Presentation] イネ緑化過程の比較プロテオーム解析.日本プロテオーム学会2012年大会2012

    • Author(s)
      柳澤修一
    • Organizer
      日本ヒトプトテオーム機構第10回大会シンポジウム"農学プロテオーム研究の最前線"
    • Place of Presentation
      東京
    • Year and Date
      2012-07-26
    • Related Report
      2013 Final Research Report
  • [Presentation] シロイヌナズナの窒素応答2012

    • Author(s)
      柳澤修一
    • Organizer
      日本学術振興会産学協力第160委員会第2回研究会「植物栄養代謝バランスの多方面からの理解」
    • Place of Presentation
      福岡
    • Year and Date
      2012-03-02
    • Related Report
      2013 Final Research Report 2010 Annual Research Report
  • [Presentation] 硝酸によるシロイヌナズナ硝酸還元酵素遺伝子NIA1の発現誘導の分子機構2012

    • Author(s)
      小西美稲子,柳澤修一
    • Organizer
      日本土壌肥料学会2012年度鳥取大会
    • Place of Presentation
      鳥取
    • Related Report
      2013 Final Research Report
  • [Presentation] 高等植物の硝酸応答機構2011

    • Author(s)
      小西美稲子,柳澤修一
    • Organizer
      日本植物学会第75回大会シンポジウム
    • Place of Presentation
      東京
    • Year and Date
      2011-09-17
    • Related Report
      2013 Final Research Report 2010 Annual Research Report
  • [Presentation] シロイヌナズナ硝酸還元酵素遺伝子NIA1の硝酸応答2010

    • Author(s)
      小西美稲子,柳澤修一
    • Organizer
      日本土壌肥料学会2010年度北海道大会
    • Place of Presentation
      札幌
    • Related Report
      2013 Final Research Report
  • [Presentation] Metabolite profiling in primary metabolism based on capillary electrophoresis-mass spectrometry and anion exchange chromatography in plant extracts2010

    • Author(s)
      Sato S., and Yanagisawa S.
    • Organizer
      日本土壌肥料学会2010年度北海道大会
    • Place of Presentation
      札幌
    • Related Report
      2013 Final Research Report
  • [Presentation] Molecular mechanisms underlying nitrate-responsive transcription2010

    • Author(s)
      Yanagisawa S
    • Organizer
      1st International Symposium on the Nitrogen Nutrition of Plants
    • Place of Presentation
      Inuyama, Japan
    • Related Report
      2013 Final Research Report
  • [Presentation] Metabolome researches in plant metabolic regulation2010

    • Author(s)
      Mimura T., Hirai M., and Yanagisawa S.
    • Organizer
      21st International Conference on Arabidopsis Research, Concurrent section "Systems biology and metabolism"
    • Place of Presentation
      Yokohama, Japan
    • Related Report
      2013 Final Research Report
  • [Presentation] 植物転写因子を用いた植物機能の強化

    • Author(s)
      柳澤修一
    • Organizer
      生物生産工学研究センターシンポジウム "植物機能のバイオテクノロジー"
    • Place of Presentation
      東京
    • Related Report
      2011 Annual Research Report
    • Invited
  • [Presentation] イネ緑化過程の比較プロテオーム解析

    • Author(s)
      柳澤修一
    • Organizer
      日本プロテオーム学会2012年大会、日本ヒトプトテオーム機構 第10回大会シンポジウム "農学プロテオーム研究の最前線"
    • Place of Presentation
      東京
    • Related Report
      2011 Annual Research Report
    • Invited
  • [Presentation] NBP転写因子による硝酸応答の制御

    • Author(s)
      小西美稲子、柳澤修一
    • Organizer
      第54回日本植物生理学会年会
    • Place of Presentation
      岡山
    • Related Report
      2011 Annual Research Report
  • [Book] Transcription factors : improving abiotic stress tolerance in plants, in Improving Stress Resistance to Abiotic Stress (Narendra Tuteja, ed.)2012

    • Author(s)
      Ishida T. Osakabe Y., Yanagisawa S.
    • Publisher
      Wiley-Blackwell, Wiley-VCH Verlag GmbH & Co. (Germany)
    • Related Report
      2013 Final Research Report
  • [Remarks] 窒素栄養の利用を制御する植物の仕組み

    • URL

      http://www.u-tokyo.ac.jp/ja/utokyo-research/tag/nin-like-transcription-factor/

    • Related Report
      2013 Final Research Report
  • [Remarks] NIN様転写因子が植物の硝酸応答を司る

    • URL

      http://www.a.u-tokyo.ac.jp/topics/2013/20130321-1.html

    • Related Report
      2013 Final Research Report

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Published: 2010-08-23   Modified: 2019-07-29  

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