| Project/Area Number |
22380045
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Plant nutrition/Soil science
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| Research Institution | Hiroshima University |
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Principal Investigator |
KUGA YUKARI 広島大学, 総合科学研究科, 教授 (30232747)
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| Co-Investigator(Kenkyū-buntansha) |
WASAKI Jun 広島大学, 大学院・生物圏科学研究科, 准教 (00374728)
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| Co-Investigator(Renkei-kenkyūsha) |
SAITO Masanori 東北大学, 農学研究科, 教授 (40355079)
YURIMOTO Hisayoshi 北海道大学, 理学研究院, 教授 (80191485)
HAYAKAWA Shinjiro 広島大学, 工学研究科, 准教授 (80222222)
NAKAMURA Hitoshi (独)農業, 食品産業技術総合研究機構・果樹研究所, 主任研究員 (80360444)
YAMANAKA Takashi (独)森林総合研究所, 森林微生物研究領域, 研究員 (00343799)
MASUNO Kazuhiko 長野県林業総合センター, 特産部, 特産部長 (50450820)
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| Project Period (FY) |
2010-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥18,200,000 (Direct Cost: ¥14,000,000、Indirect Cost: ¥4,200,000)
Fiscal Year 2013: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Fiscal Year 2012: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
Fiscal Year 2011: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
Fiscal Year 2010: ¥9,750,000 (Direct Cost: ¥7,500,000、Indirect Cost: ¥2,250,000)
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| Keywords | 菌根共生 / 物質輸送 / 同位体顕微鏡 / 微細構造 / 菌糸圏微生物 / PCR-DGGE / 白紋羽病 / 温水処理 / 分析電顕(EF-TEM) / 蛍光X線分析 |
|---|
| Research Abstract |
Objectives of this study were to establish a new methodology to analyze elemental flows between organisms at levels of cell, tissue, and soil ecosystems. At cellular and tissue levels, mycorrhizal systems were used, where localizations of stable isotope tracers were observed by an isotope microscope in resin embedded sections. For soil microbe interactions, a decline of soil born fungus causing white root rot by a hot water treatment was used as a model system. It was shown that soil microbes participated in the fungal death. Methods to analyze bacteria communities of mycosphere, and inoculation tests, proliferation and antifungal tests on fungal hyphae, with a heat treatment were established for bacterial isolates.
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