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Molecular mechanisms underlying the sorting of bacterial lipoproteins.

Research Project

Project/Area Number 22380049
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Applied microbiology
Research InstitutionMorioka College

Principal Investigator

TOKUDA Hajime  盛岡大学, 栄養学部, 教授 (40125943)

Project Period (FY) 2010-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥16,640,000 (Direct Cost: ¥12,800,000、Indirect Cost: ¥3,840,000)
Fiscal Year 2014: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Fiscal Year 2013: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Fiscal Year 2012: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2011: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Fiscal Year 2010: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
KeywordsLolシステム / リポ蛋白質 / 膜局在化 / ペリプラズム / 分子シャペロン / 細胞表層構造 / ABCトランスポーター / 阻害剤 / エンベロープストレス / Lo1システム / 膜局在 / 光化学架橋
Outline of Final Research Achievements

The Lol system catalyzing the sorting of lipoproteins from the inner to outer membranes was studied and the following five major findings were obtained. (1) LolC and LolD are functionally different to each other although their structures are similar. (2) Detailed molecular events involving lipoproteins, LolCDE, LolA, and LolB were revealed. (3) Defective lipoprotein sorting induces the Rcs stress response system. (4) The site of LolB required for membrane targeting was identified. (5) Chemicals, which inhibit the function of LolC and LolE, were found.

Report

(6 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Annual Research Report
  • 2012 Annual Research Report
  • 2011 Annual Research Report
  • 2010 Annual Research Report
  • Research Products

    (23 results)

All 2015 2014 2013 2012 2011 2010 Other

All Journal Article (13 results) (of which Peer Reviewed: 13 results) Presentation (3 results) (of which Invited: 1 results) Book (3 results) Remarks (4 results)

  • [Journal Article] Small molecule inhibitors of Gram-negative lipoprotein trafficking discovered by phenotypic screening.2015

    • Author(s)
      McLeod, S. M., Fleming, P. R., MacCormack, K., McLaughlin, R. E., Whiteaker, J. D., Narita, S., Mori, M., Tokuda, H., and Miller, A. A.
    • Journal Title

      J. Bacteriol.

      Volume: 197 Issue: 6 Pages: 1075-1082

    • DOI

      10.1128/jb.02352-14

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Brown, D. G., and McLeod, S. M. Novel antibacterial targets and compounds revealed by a high throughput cell wall reporter assay.2015

    • Author(s)
      Nayar, A. S., Dougherty, T. J., Ferguson, K. E., Granger, B. A., McWilliams, L., Stacey, C., Leach, L. J., Narita, S., Tokuda, H., Miller, A. A.
    • Journal Title

      J. Bacteriol.

      Volume: 197

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Roles of the protruding loop of factor B essential for the localization of lipoproteins (LolB) in the anchoring of bacterial triacylated proteins to the outer membrane.2014

    • Author(s)
      Hayashi, Y., Tsurumizu, R., Tsukahara, J., Takeda, K., Narita, S., Mori, M., Miki, K., and Tokuda, H.
    • Journal Title

      J. Biol. Chem.

      Volume: 289 Issue: 15 Pages: 10530-10539

    • DOI

      10.1074/jbc.m113.539270

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Functional differentiation of structurally similar membrane subunits of the ABC transporter LolCDE complex.2013

    • Author(s)
      Mizutani, M., Mukaiyama, K., Xiao, J., Mori, M., Satou, R., Narita, S., Okuda, S., and Tokuda, H.
    • Journal Title

      FEBS Lett.

      Volume: 587 Issue: 1 Pages: 23-29

    • DOI

      10.1016/j.febslet.2012.11.009

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Glycolipozyme MPIase is essential for topology inversion of SecG during preprotein translocation2012

    • Author(s)
      Moser, M., Nagamori, S., Huber, M., Tokuda, H., Nishiyama, K.
    • Journal Title

      Proc. Natl. Acad. Sci. USA

      Volume: (in press) Issue: 24 Pages: 9734-9739

    • DOI

      10.1073/pnas.1303160110

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Defective lipoprotein sorting induces lolA expression through the Rcs stress response phosphorelay system.2012

    • Author(s)
      Tao, K., Narita, S., and Tokuda, H.
    • Journal Title

      J. Bacteriol.

      Volume: (in press) Issue: 14 Pages: 3643-3650

    • DOI

      10.1128/jb.00553-12

    • Related Report
      2012 Annual Research Report 2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] MPIase is a glycolipozyme essential for membrane protein integration2012

    • Author(s)
      Nishiyama, K., Maeda,M., Yanagisawa,K., Nagase, R., Komura, H., Iwashita, T., Yamagaki, T., Kusumoto, S., Tokuda, H., Shimamoto, K.
    • Journal Title

      Nat. Commun

      Volume: 3 Issue: 1 Pages: 1-10

    • DOI

      10.1038/ncomms2267

    • NAID

      120007179832

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Multiple SecA molecules drive protein translocation across a single translocon with SecG inversion2012

    • Author(s)
      Morita, K., Tokuda, H. and Nishiyama, K.
    • Journal Title

      J. Biol. Chem

      Volume: 287巻 Issue: 1 Pages: 455-464

    • DOI

      10.1074/jbc.m111.301754

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Lipoprotein Sorting in Bacteria2011

    • Author(s)
      Okuda, S., Tokuda, H.
    • Journal Title

      Annu.Rev.Microbiol.

      Volume: 65 Pages: 239-259

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Overexpression of LolCDE allows deletion of the Escherichia coli gene encoding apolipoprotein N-acyltransferase2011

    • Author(s)
      Narita, S., Tokuda, H.
    • Journal Title

      J.Bacteriol.

      Volume: 193 Pages: 4832-4840

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Biogenesis and membrane targeting of lipoproteins2010

    • Author(s)
      Narita, S., Tokuda, H.
    • Journal Title

      Escherichia coli and Salmonella ; cellular and molecular biology

      Volume: Chapter4.3.7(online journal)

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Novel mutations of the LolCDE complex causing outer membrane localization of lipoproteins despite their inner membrane retention signals2010

    • Author(s)
      Sakamoto, C, Satou, R., Tokuda, H., Narita, S.
    • Journal Title

      Biochem.Biophys.Res.Commun.

      Volume: 401 Pages: 586-591

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A periplasmic LolA derivative with a lethal disulfide bond activates the Cpx stress response system2010

    • Author(s)
      Tao, K., Watanabe, S., Narita, S., Tokuda, H.
    • Journal Title

      J.Bacteriol.

      Volume: 192 Pages: 5657-5662

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Presentation] 生育条件の変化に応答したLpp-ペプチドグリカントランスペプチダーゼ遺伝子の発現調節2012

    • Author(s)
      青木将、福本玲、徳田元、松山伸一
    • Organizer
      日本農芸化学会
    • Place of Presentation
      京都女子大学
    • Year and Date
      2012-03-24
    • Related Report
      2011 Annual Research Report
  • [Presentation] 多彩な機能を有するリポ蛋白質の膜局在化を触媒するLol経路

    • Author(s)
      徳田 元
    • Organizer
      日本農芸化学会
    • Place of Presentation
      東北大学
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Presentation] 大腸菌外膜リポ蛋白質輸送不全によるRcsリン酸リレー系に依存したlolA転写活性化の機構

    • Author(s)
      垰 和之、徳田 元
    • Organizer
      日本農芸化学会
    • Place of Presentation
      東北大学
    • Related Report
      2012 Annual Research Report
  • [Book] Bacterial Membranes: Structural and Molecular Biology2014

    • Author(s)
      Tokuda, H., Sander, P., Lee, B. L., Okuda, S., Grau, T., Tschumi, A., Brülle, J. K., Kurokawa, K., and Nakayama, H.
    • Total Pages
      500
    • Publisher
      Caiser Academic Press
    • Related Report
      2013 Annual Research Report
  • [Book] Bacterial Lipoproteins ; Biogenesis, Trafficking and Function2012

    • Author(s)
      Tokuda, H., 他8名
    • Publisher
      Horizon Scientific Press(in press)
    • Related Report
      2011 Annual Research Report
  • [Book] Methods in Molecular Biology ; Protein Secretion2010

    • Author(s)
      Narita, S., Tokuda, H.
    • Publisher
      Sorting of bacterial lipoproteins to the outer membrane by the Lol system
    • Related Report
      2010 Annual Research Report
  • [Remarks] 栄養科学部栄養科学科徳田研究室

    • URL

      http://www.morioka-u.ac.jp/UV_ns/tokuda_lab/study.html

    • Related Report
      2014 Annual Research Report
  • [Remarks] 徳田研究室ホームページ

    • URL

      http://www.morioka-u.ac.jp/UV_ns/tokuda_lab/

    • Related Report
      2012 Annual Research Report
  • [Remarks]

    • URL

      http://www.morioka-u.ac.jp/UV_ns/tokuda_lab/

    • Related Report
      2011 Annual Research Report
  • [Remarks]

    • URL

      http://www.morioka-u.ac.jp/UV_ns/tokuda_lab/

    • Related Report
      2010 Annual Research Report

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Published: 2010-08-23   Modified: 2019-07-29  

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