| Budget Amount *help |
¥17,940,000 (Direct Cost: ¥13,800,000、Indirect Cost: ¥4,140,000)
Fiscal Year 2012: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2011: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2010: ¥7,280,000 (Direct Cost: ¥5,600,000、Indirect Cost: ¥1,680,000)
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| Research Abstract |
Rhodococcus jostii RHA1 is a strong degrader of the environmental pollutant, polychlorinated biphenyls (PCBs). Induction of its degradation enzymes requires an inducing substrate and is conducted by a couple of two-component transcriptional regulators, BphS1T1 and BphS2T2. Mechanism of transcriptional regulation of BphS1T1 and BphS2T2 were examined to develop a strain whose PCB-degradation enzymes are induced by PCBs themselves. Phosphorylation of BphT (BphT1/T2) in the presence of the inducing substrate was indicated. A novel 24-bp consensus sequence was shared among the degradation enzyme gene operon promoters and had necessary and sufficient function for transcriptional induction by BphST, suggesting that the 24-bp sequence serves the binding site for the transcriptional complex including BphT.
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