Molecular circulation and carbon fixation by C1-microorganism consortia

• Project/Area Number: 22380052
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Applied microbiology
• Research Institution: Kyoto University
• Principal Investigator: SAKAI Yasuyoshi 京都大学, 大学院・農学研究科, 教授 (60202082)
• Co-Investigator(Kenkyū-buntansha): YURIMOTO Hiroya 京都大学, 大学院・農学研究科, 准教授 (00283648) ; OKU Masahide 京都大学, 大学院・農学研究科, 助教 (10511230)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥18,200,000 (Direct Cost: ¥14,000,000、Indirect Cost: ¥4,200,000); Fiscal Year 2012: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2011: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2010: ¥7,800,000 (Direct Cost: ¥6,000,000、Indirect Cost: ¥1,800,000)
• Keywords: メタン / メタノール / メタン酸化菌 / 生育促進 / レドックス / オートファジー / 複合生物系 / メタノール資化性細菌 / メタン資化性細菌 / 生長促進 / グルタチオン

Research Abstract

In order to strengthen carbon fixation in global carbon cycle, we studied the useful functions of C1-microorganism consortia including methane-and methanol-utilizing microorganisms and molecular mechanism of molecular and energy circulation. We revealed distribution of C1-microorganisms and foundnovel functions necessary for proliferation at phyllosphere.

Research Products

• [Journal Article] Hyper-activation of target of rapamycin (TOR) kinase 1 decreases intracellular glutathione content in Saccharomyces cerevisiaerevealed by LC-MS/MS analysis (2013)
  • Author(s): M. Oku, Y. Ichiki, A. Shiraishi, T. Takahara, T. Maeda,and Y. Sakai
  • Journal Title: Biosci. Biotechnol. Biochem Volume: (in press)
  • Peer Reviewed
• [Journal Article] Dominant colonization and inheritance of Methylobacteriumsp. strain OR01 on perilla plants (2013)
  • Author(s): M. Mizuno, H. Yurimoto, H. Iguchi, A. Tani, and Y. Sakai
  • Journal Title: Biosci. Biotechnol. Biochem. Volume: (in press)
  • NAID: 10031190465
  • Peer Reviewed
• [Journal Article] fluorescence resonance energy transfer (FRET)-based redox sensor reveals physiological role of thioredoxin in the yeast Saccharomyces cerevisiae (2013)
  • Author(s): M. Oku, J. Hoseki, Y. Ichiki, and Y. Sakai.A
  • Journal Title: FEBS Lett. Volume: 587 Issue: 6 Pages: 793-798
  • DOI: 10.1016/j.febslet.2013.02.003
  • NAID: 120005244260
  • Peer Reviewed
• [Journal Article] Primary and secondary metabolism regulates lipolysis in appressoria of Colletotrichum orbiculare (2012)
  • Author(s): M. Asakura, K. Yoshino, A.M.. Hill, Y. Kubo, Y. Sakai, and Y. Takano.
  • Journal Title: Fungal Genet. Biol Volume: 49 Issue: 11 Pages: 967-97
  • DOI: 10.1016/j.fgb.2012.08.009
  • Peer Reviewed
• [Journal Article] 異常を回復する化合物レドックスモジュレーターの探索:Redoxfluorの創薬への利用 (2012)
  • Journal Title: 遺伝子医学MOOK Volume: 22 Pages: 158-163
• [Journal Article] Molecular characterization of the Candida boidinii MIG1and its role in regulation of methanol inducible gene expression (2012)
  • Author(s): Z. Zhai, H. Yurimoto, and Y. Sakai
  • Journal Title: Yeast Volume: 29 Issue: 7 Pages: 293-301
  • DOI: 10.1002/yea.2909
  • Peer Reviewed
• [Journal Article] Distribution of Methanotrophs in the Phyllosphere (2012)
  • Author(s): H. Iguchi, I. Sato, M. Sakakibara, H. Yurimoto, and Y. Sakai
  • Journal Title: Bioscience, Biotechnology, and Biochemistry Volume: 76 Issue: 8 Pages: 1580-1583
  • DOI: 10.1271/bbb.120281
  • NAID: 10031066412
  • ISSN: 0916-8451, 1347-6947
  • Peer Reviewed
• [Journal Article] Distribution of Pink-Pigmented Facultative Methylotrophs on Leaves of Vegetables (2012)
  • Author(s): M. Mizuno, H. Yurimoto, N. Yoshida, H. Iguchi, and Y. Sakai
  • Journal Title: Bioscience, Biotechnology, and Biochemistry Volume: 76 Issue: 3 Pages: 578-580
  • DOI: 10.1271/bbb.110737
  • NAID: 10030750921
  • ISSN: 0916-8451, 1347-6947
  • Peer Reviewed
• [Journal Article] Msn5p Is Involved in Formaldehyde Resistance but Not in Oxidative Stress Response in the Methylotrophic Yeast Candida boidinii (2012)
  • Author(s): Z. Zhai, H. Yurimoto, and Y. Sakai
  • Journal Title: Bioscience, Biotechnology, and Biochemistry Volume: 76 Issue: 2 Pages: 299-304
  • DOI: 10.1271/bbb.110679
  • NAID: 10030399766
  • ISSN: 0916-8451, 1347-6947
  • Peer Reviewed
• [Journal Article] Assessment of physiological redox state with FRET protein probes. Antioxid. (2012)
  • Author(s): M. Okuand Y. Sakai.
  • Journal Title: Redox Signal Volume: 16 Issue: 7 Pages: 698-704
  • DOI: 10.1089/ars.2011.4251
  • Peer Reviewed
• [Journal Article] レドックス異常を回復する化合物レドックスモジュレーターの探索：Redoxfluorの創薬への利用 (2012)
  • Author(s): 阪井康能、寳関 淳、奥 公秀
  • Journal Title: 遺伝子医学MOOK Volume: 22 Pages: 158-163
• [Journal Article] Stimulation of methanotrophic growth in co-cultures by cobalamin excreted by rhizobia. (2011)
  • Author(s): H. Iguchi, H. Yurimoto, and Y. Sakai
  • Journal Title: Appl. Environ. Microbiol Volume: 77 Issue: 24 Pages: 8509-8515
  • DOI: 10.1128/aem.05834-11
  • NAID: 120003692388
  • Peer Reviewed
• [Journal Article] Yeast methylotrophy and autophagy in a methanol-oscillating environment on growing Arabidopsis thaliana leaves. (2011)
  • Author(s): K. Kawaguchi, H. Yurimoto, M. Oku, and Y. Sakai
  • Journal Title: PLoS ONE Volume: 6 Issue: 9 Pages: e25257-e25257
  • DOI: 10.1371/journal.pone.0025257
  • NAID: 120003367881
  • Peer Reviewed
• [Journal Article] 細胞内の酸化還元状態を検知するタンパク質FRETプローブ, レドックスフロール 酵母の酸化ストレス応答から学び, それを利用する新戦略 (2011)
  • Author(s): 奥 公秀、阪井康能
  • Journal Title: 化学と生物 Volume: 49 Pages: 516-517
  • NAID: 10029592104
• [Journal Article] C1 微生物複合生物系による省エネ型炭素固定技術. (2011)
  • Author(s): 阪井康能、由里本博也
  • Journal Title: 環境資源工学 Volume: 58 Pages: 59-63
• [Journal Article] Inhibition of surgical trauma-enhanced peritoneal dissemination of tumor cells by human catalase derivatives in mice (2011)
  • Author(s): C. Nishizaki, M. Nishikawa, T. Yata, T. Yamada, Y. Takahashi, M. Oku, H. Yurimoto, Y. Sakai,K. Nakanishi, and Y. Takakura
  • Journal Title: Free Radic. Biol. Med Volume: 51 Issue: 3 Pages: 773-779
  • DOI: 10.1016/j.freeradbiomed.2011.05.025
  • NAID: 120003255726
  • Peer Reviewed
• [Journal Article] Yeast methylotrophy: metabolism, gene regulation, and peroxisome homeostasis (2011)
  • Author(s): H. Yurimoto, M. Oku, and Y. Sakai
  • Journal Title: Int. J. Microbiol Volume: 2011 Pages: 101298-101298
  • DOI: 10.1155/2011/101298
  • NAID: 120003405543
  • Peer Reviewed
• [Journal Article] Methylovulum miyakonense gen. nov., sp. Nov., a novel type I methanotroph from a forest soil. (2011)
  • Author(s): H. Iguchi, H. Yurimoto, and Y. Sakai
  • Journal Title: Int. J. Syst. Evol. Microbiol. Volume: 61 Issue: 4 Pages: 810-815
  • DOI: 10.1099/ijs.0.019604-0
  • Peer Reviewed
• [Journal Article] Redoxfluorを用いた細胞内酸化還元状態の可視化 (2011)
  • Author(s): 奥 公秀、阪井康能
  • Journal Title: 実験医学 Volume: 29 Pages: 945-950
• [Journal Article] C1微生物複合生物系による省エネ型炭素固定技術 (2011)
  • Author(s): 阪井康能、由里本博也
  • Journal Title: 環境資源工学 Volume: 58 Pages: 59-63
• [Journal Article] 細胞内の酸化還元状態を検知するタンパク質FRETプローブ,レドックスフロール酵毋の酸化ストレス広答から学び,それを利用する新戦略 (2011)
  • Author(s): 奥公秀、阪井康能
  • Journal Title: 化学と生物 Volume: 49 Pages: 516-517
• [Journal Article] Methylovulum miyakonense gen.nov., sp.nov., a novel type I methanotroph from a forest soil. (2011)
  • Author(s): H.Iguchi, H.Yurimoto, Y.Sakai.
  • Journal Title: Int.J.Syst.Evol.Microbiol. Volume: 61 Pages: 810-815
  • Peer Reviewed
• [Journal Article] C1微生物と植物の相互作用と炭素循環 ‐代謝生化学・分子細胞生物学からの理解‐ (2010)
  • Author(s): 由里本博也、阪井康能
  • Journal Title: 植物の生長調節 Volume: 45 Pages: 125-131
• [Journal Article] Atg8 regulates vacuolar membrane dynamics in a lipidation-independent manner in Pichia pastoris (2010)
  • Author(s): N. Tamura, M. Oku, and Y. Sakai
  • Journal Title: J. Cell Sci Volume: 123 Issue: 23 Pages: 4107-4116
  • DOI: 10.1242/jcs.070045
  • Peer Reviewed
• [Journal Article] Crystal structure of 3-hexulose- 6-phosphate synthase, a member of the orotidine 5'-monophosphate decarboxylase suprafamily (2010)
  • Author(s): I. Orita, A. Kita, H. Yurimoto, N. Kato, Y. Sakai, and K. Miki
  • Journal Title: Proteins Volume: 78 Issue: 16 Pages: 3488-3492
  • DOI: 10.1002/prot.22860
  • Peer Reviewed
• [Journal Article] Soluble and particulate methane monooxygenase gene clusters in the type I methanotrophMethylovulum miyakonense HT12. (2010)
  • Author(s): H. Iguchi, H. Yurimoto, and Y. Sakai
  • Journal Title: FEMS Microbiol. Lett. Volume: 312 Issue: 1 Pages: 71-76
  • DOI: 10.1111/j.1574-6968.2010.02101.x
  • Peer Reviewed
• [Journal Article] Peroxisomes as dynamic organelles: autophagic degradation (2010)
  • Author(s): Oku, M. and Sakai, Y
  • Journal Title: FEBS J Volume: 277 Issue: 16 Pages: 3289-3294
  • DOI: 10.1111/j.1742-4658.2010.07741.x
  • Peer Reviewed
• [Journal Article] novel fluorescent sensor protein for visualization of redox states in the cytoplasm and in peroxisomes (2010)
  • Author(s): T. Yano, M. Oku, N. Akeyama, A. Itoyama, H. Yurimoto, S. Kuge, Y. Fujiki, and Y. Sakai.A
  • Journal Title: Mol. Cell. Biol Volume: 30 Issue: 15 Pages: 3758-3766
  • DOI: 10.1128/mcb.00121-10
  • NAID: 120002182833
  • Peer Reviewed
• [Journal Article] Trm2p-dependent derepression is essential for methanol-specific gene activation in the methylotrophic yeast Candida boidinii. (2010)
  • Author(s): Y. Sasano, H. Yurimoto, M. Kuriyama, and Y. Sakai
  • Journal Title: FEMS Yeast Res Volume: 10 Pages: 535-544
  • DOI: 10.1111/j.1567-1364.2010.00640.x
  • Peer Reviewed
• [Journal Article] Trm2p-dependent derepression is essential for methanol-specific gene activation in the methylotrophic yeast Candida boidinii (2010)
  • Author(s): Y.Sasano, H.Yurimoto, M.Kuriyama, Y.Sakai.
  • Journal Title: FEMS Yeast Res. Volume: 10 Pages: 535-544
  • Peer Reviewed
• [Journal Article] A novel fluorescent sensor protein for visualization of redox states in the cytoplasm and in peroxisomes. (2010)
  • Author(s): T.Yano, M.Oku, N.Akeyama, A.Itoyama, H.Yurimoto, S.Kuge, Y.Fujiki, Y.Sakai.
  • Journal Title: Mol.Cell.Biol. Volume: 30 Pages: 3758-3766
  • NAID: 120002182833
  • Peer Reviewed
• [Journal Article] Peroxisomes as dynamic organelles: autophagic degradation. (2010)
  • Author(s): Oku, M., Sakai, Y.
  • Journal Title: FEBS J. Volume: 277 Pages: 3289-3294
  • Peer Reviewed
• [Journal Article] Soluble and particulate methane monooxygenase gene clusters in the type I methanotroph Methylovulum miyakonense HT12. (2010)
  • Author(s): H.Iguchi, H.Yurimoto, Y.Sakai.
  • Journal Title: FEMS Microbiol.Lett. Volume: 312 Pages: 71-76
  • Peer Reviewed
• [Journal Article] Crystal structure of 3-hexulose-6-phosphate synthase, a member of the orotidine 5'-monophosphate decarboxylase suprafamily. (2010)
  • Author(s): I.Orita, A.Kita, H.Yurimoto, N.Kato, Y.Sakai, K.Miki.
  • Journal Title: Proteins Volume: 78 Pages: 3488-3492
  • Peer Reviewed
• [Journal Article] Atg8 regulates vacuolar membrane dynamics in a lipidation-independent manner in Pichia pastoris. (2010)
  • Author(s): N.Tamura, M.Oku, Y.Sakai.
  • Journal Title: J.Cell Sci. Volume: 123 Pages: 4107-4116
  • Peer Reviewed
• [Journal Article] C1微生物と植物の相互作用と炭素循環 -代謝生化学・分子細胞生物学からの理解- (2010)
  • Author(s): 由里本博也、阪井康能
  • Journal Title: 植物の生長調節 Volume: 45 Pages: 125-131
• [Presentation] 生理的な細胞内レドックス状態を可視化するRedoxfluor (2013)
  • Author(s): 阪井康能
  • Organizer: 第54回日本植物生理学会年会シンポジウム「レドックス恒常性と制御」
  • Place of Presentation: 岡山大学（岡山市）
  • Invited
• [Presentation] 生理的な細胞内レドックス状態を可視化するFRETプローブとその生命・創薬科学への利用 (2012)
  • Author(s): 阪井康能
  • Organizer: レドックスライフイノベーションシンポジウム
  • Place of Presentation: 東京大学(招待講演)
  • Year and Date: 2012-03-09
• [Presentation] PpAtg18 mediates switch of vacuolar dynamics between micro-and macropexophagy (2012)
  • Author(s): Sakai, Y
  • Organizer: Gordon Research Conference on Autophagy in Stress, Development and Disease
  • Place of Presentation: Ventura, USA
• [Presentation] 細胞内ダイナミクスの分子機構と自然界での高次機能 ～C1酵母&植物病原菌共同研究から得たヒント～ (2012)
  • Author(s): 阪井康能
  • Organizer: 第２０回酵母合同シンポジウム
  • Place of Presentation: 京都大学（宇治市）
  • Invited
• [Presentation] C1微生物代謝の省エネ型炭素固定系としての利用とその問題点 (2012)
  • Author(s): 阪井康能、由里本博也
  • Organizer: 第６４回日本生物工学会大会シンポジウム「日本から発信する、エネルギー革新省エネ型炭素固定とe-バイオの融合」
  • Place of Presentation: 神戸国際会議場（神戸市）
  • Invited
• [Presentation] PpAtg18 mediates switch of vacuolar dynamics between micro- and macropexophagy (2012)
  • Author(s): Yasuyoshi Sakai
  • Organizer: Gordon conference "Autophagy in Stress, Development and Disease"
  • Place of Presentation: Ventura(米国)
• [Presentation] Methylotrophy and autophagy: from genome and molecules to yeast life style in nature (2011)
  • Author(s): Sakai, Y
  • Organizer: International Symposium on Microbial Genomic Research
  • Place of Presentation: Seoul, Korea
  • Year and Date: 2011-12-02
• [Presentation] Methylotrophy and Autophagy : From Genome & Molecules To Yeast Life Style in Nature (2011)
  • Author(s): Yasuyoshi Sakai
  • Organizer: International Symposium "Microbial Genomic Research : A Fronter for Future Green Biotechnology"
  • Place of Presentation: ソウル(韓国)(招待講演)
  • Year and Date: 2011-12-02
• [Presentation] C1微生物-植物複合系による炭素循環:その原理とバイオマスへの再資源化固定 (2011)
  • Author(s): 阪井康能
  • Organizer: 京都大学微生物科学寄附研究部門主催シンポジウム
  • Place of Presentation: 京都大学(招待講演)
  • Year and Date: 2011-06-23
• [Presentation] Regulation and physiological role of autophagy in methylotrophic yeast. (2011)
  • Author(s): Sakai, Y
  • Organizer: he 2nd Sino-Japan Autophagy Symposium.
  • Place of Presentation: Kanagawa, Japan
• [Presentation] C1-metabolism coupled gene expression and molecular ecology at phyllosphere (2011)
  • Author(s): Sakai, Y
  • Organizer: International Union of Microbilogical Societies 2011 Congress
  • Place of Presentation: Sapporo, Japan
• [Presentation] C1-metabolism coupled gene expression and molecular ecology at phyllosphere (2011)
  • Author(s): Yasuyoshi Sakai
  • Organizer: International Union of Microbiological Societies 2011 Congress
  • Place of Presentation: 札幌コンベンションセンター(招待講演)
• [Presentation] Regulation and physiological role of autophagy in the methylotrophic yeast (2011)
  • Author(s): Yasuyoshi Sakai
  • Organizer: The 2nd Sino-Japan Autophagy Symposium
  • Place of Presentation: 湘南国際村センター(神奈川)(招待講演)
• [Presentation] 生体キノン化合物から資源環境問題へのアプローチ (2010)
  • Author(s): 阪井康能
  • Organizer: 生体キノン研究会第9回講演会
  • Place of Presentation: 中央大学(東京都)(招待講演)
  • Year and Date: 2010-09-17
• [Presentation] Regulation and molecular basis for membrane dynamics of micropexophagy. (2010)
  • Author(s): Sakai, Y
  • Organizer: The 1st Sino-Japan Autophagy Symposium
  • Place of Presentation: Xi'an, China
• [Presentation] Regulation and molecular basis for membrane dynamics of micropexophagy (2010)
  • Author(s): Sakai, Y
  • Organizer: SwissYeast Meeting
  • Place of Presentation: Fribourg, Switzerland
• [Presentation] Methane-utilizing microbial consortium established from a forest soil including Methylovulum miyakonense gen. nov., sp. nov., a novel type I methanotroph (2010)
  • Author(s): Sakai, Y
  • Organizer: Gordon Research Conference on Molecula Basis of Microbial One-Carbon Metabolism
  • Place of Presentation: Lewiston, USA
• [Presentation] Recruitment of PI4-kinase from the Golgi apparatus to PAS. (2010)
  • Author(s): Sakai, Y
  • Organizer: Gordon Research Conference on Autophagy in Stress, Development and Disease
  • Place of Presentation: Lucca, Italy
• [Presentation] Recruitment of PI4-kinase from the Golgi appratus to PAS (2010)
  • Author(s): Sakai, Y.
  • Organizer: Gordon Research Conference on Autophagy in Stress, Development and Disease
  • Place of Presentation: Lucca (Italy)(招待講演)
• [Presentation] 細胞内レドックス状態を可視化するFRETプローブの開発 (2010)
  • Author(s): 阪井康能
  • Organizer: 第19回酵母合同シンポジウム
  • Place of Presentation: 東京大学(東京都)(招待講演)
• [Presentation] Methane-utilizing microbial consortium established from a forest soil including Methylovulum miyakonense gen.nov., sp.nov., a novel type I methanotroph. (2010)
  • Author(s): Sakai, Y.
  • Organizer: Gordon Research Conference on Molecular Basis of Microbioal One-Carbon Metabolism
  • Place of Presentation: Lewiston, ME (USA)
• [Presentation] Regulation and Molecular Basis for Membrane Dynamics of Microautophagy (2010)
  • Author(s): Sakai, Y.
  • Organizer: Swiss Yeast Meeting
  • Place of Presentation: Fribourg (Switzerland)(招待講演)
• [Presentation] Regulation and Molecular Basis for Membrane Dynamics of Microautophagy (2010)
  • Author(s): Sakai, Y.
  • Organizer: The 1st Sino-Japan Autophagy Symposium
  • Place of Presentation: 西安(中国)(招待講演)
• [Presentation] Carbon fixation and bioremediation by fusion of microbilal and plant functions (2010)
  • Author(s): Sakai, Y.
  • Organizer: The 2nd Workshop Argentina-Japan "Bioscience and Biotechnology for the Promotion of Agriculture and Food Prodction"
  • Place of Presentation: 東京(招待講演)
• [Presentation] メタノールを介する植物-微生物間相互作用 ～分子細胞生物学からの理解を目指して～
  • Author(s): 阪井康能
  • Organizer: 第７回 京都大学植物縦横無尽の会 ワークショップ
  • Place of Presentation: 京都大学（京都市）
  • Invited
• [Presentation] 葉上に棲息するC1微生物-植物間相互作用と日周変動
  • Author(s): 阪井康能、由里本博也
  • Organizer: 第216回生存圏シンポジウム「植物と微生物：C1化合物を介した気候変動との関わりの理解に向けて」
  • Place of Presentation: 京都大学（宇治市）
  • Invited
• [Presentation] C1酵母による異種遺伝子発現を制御発酵から考える
  • Author(s): 阪井康能、由里本博也
  • Organizer: 日本生物工学会関西支部第102回醗酵学懇話会
  • Place of Presentation: 月桂冠株式会社（京都市）
  • Invited
• [Patent(Industrial Property Rights)] メタン酸化菌に対して用いられるメタン酸化活性向上剤、及びその利用 (2010)
  • Inventor(s): 阪井康能、井口博之、由里本博也
  • Industrial Property Rights Holder: 京都大学
  • Industrial Property Number: 2010-183287
  • Filing Date: 2010-08-18